MIA_01792_1

Protein

Length

397 amino acids

Browser: contig02:1692046-1693359+

Protein function

EGGNOG:	0PHE0	PGUG_02088	transcription initiation factor
SGD closest match:	S000003237	TFG2	Transcription initiation factor IIF subunit beta
CGD closest match:	CAL0000197597	orf19.2111	Transcription factor IIF subunit

Protein alignments

	%id	Aln length	E-value
MCA_02813_1	60.900%	289	1.54e-110	MCA_02813_1
A0A167C0J4_9ASCO	50.852%	352	6.69e-108	Transcription factor IIF subunit TFG2 OS=Sugiyamaella lignohabitans GN=TFG2 PE=4 SV=1
UniRef50_A0A167C0J4	50.852%	352	1.84e-104	Transcription factor IIF subunit TFG2 n=1 Tax=Sugiyamaella lignohabitans TaxID=796027 RepID=A0A167C0J4_9ASCO
A0A0J9X9M5_GEOCN	60.976%	287	9.22e-107	Similar to Saccharomyces cerevisiae YGR005C TFG2 TFIIF (Transcription Factor II) middle subunit OS=Geotrichum candidum GN=BN980_GECA05s02837g PE=4 SV=1
A0A060T2B2_BLAAD	55.282%	284	2.46e-103	ARAD1A06930p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1A06930g PE=4 SV=1
A0A1E3PPV0_9ASCO	50.149%	335	3.46e-96	Uncharacterized protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_45422 PE=4 SV=1
T2FB_YEAST	39.612%	361	2.08e-77	Transcription initiation factor IIF subunit beta OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TFG2 PE=1 SV=2
Q6C1J3_YARLI	48.252%	286	3.16e-77	YALI0F15785p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_F15785g PE=4 SV=1
A0A1E4TAM5_9ASCO	44.444%	288	5.61e-70	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_3446 PE=4 SV=1
A0A1D8PG13_CANAL	42.715%	302	1.56e-59	Transcription factor IIF subunit OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=orf19.2111 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.1068

Protein family membership

Transcription initiation factor IIF, beta subunit (IPR003196)

Domains and repeats

Domain
53 - 153
Transcription Factor IIF, Rap30/Rap74, interaction (IPR011039)
277 - 344
Winged helix-turn-helix DNA-binding domain (IPR011991)

1 50 100 150 200 250 300 350 397

Detailed signature matches

IPR003196

Transcription initiation factor IIF, beta subunit
1. PF02270 (TFIIF_beta)
  
  57 - 342
  
  Pfam
  PF02270 (TFIIF_beta)
IPR011039

Transcription Factor IIF, Rap30/Rap74, interaction
1. SSF50916 (Rap30/74 ...)
  
  53 - 153
  
  SUPERFAMILY
  SSF50916 (Rap30/74 interaction domains)
IPR011991

Winged helix-turn-helix DNA-binding domain
1. G3DSA:1.10.10.10
  
  277 - 344
  
  GENE3D
  G3DSA:1.10.10.10 (G3DSA:1.10.10.10)
2. SSF46785 ("Winged h...)
  
  278 - 343
  
  SUPERFAMILY
  SSF46785 ("Winged helix" DNA-binding domain)

no IPR

Unintegrated signatures

cd07980 (TFIIF_beta)

60 - 210

CDD
cd07980 (TFIIF_beta)
mobidb-lite (disord...)

148 - 176

MobiDB Lite
mobidb-lite (disorder_prediction)

1 - 57

MobiDB Lite
mobidb-lite (disorder_prediction)

347 - 397

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

heterodimer interf...

62K

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

64D

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

67V

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

68W

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

69L

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

70V

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

71R

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

72L

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

73P

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

74K

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

77M

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

80W

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

83H

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

90N

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

91L

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

92G

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

93K

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

94V

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

95M

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

96I

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

97H

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

98N

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

106Q

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

136D

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

137N

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

139N

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

140T

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

141F

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

142V

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

143F

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

144T

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

145E

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

146K

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

147D

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

196T

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

198L

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

199V

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

205E

heterodimer interface
62K, 64D, 67V, 68W, 69L, 70V, 71R, 72L, 73P, 74K, 77M, 80W, 83H, 90N, 91L, 92G, 93K, 94V, 95M, 96I, 97H, 98N, 106Q, 136D, 137N, 139N, 140T, 141F, 142V, 143F, 144T, 145E, 146K, 147D, 196T, 198L, 199V, 205E
CDD
cd07980 (TFIIF_beta)

Protein sequence

>MIA_01792_1
MAPNVAIKQEQKDEMLERSNSSGSSLVNGNRTSINNNGNNLDDNEEDDLEDDEDSLDLDTSKADTKVWLVRLPKFLMEKW
RDHEKLHGQNLGKVMIHNNKSNPSEQKWDVKLLLNDTPETQDIPREYDIMLAEQRDNKNTFVFTEKDLPKYDKKKPQDAT
SVNARQQQQQHSRRGGRFQFNKDRYVPYVKTIPKRTALVGKVYHECNVIPSLTDPNYNKVISSRRRLQEEEPRPKVTLLS
DSLEVSSGAFSQTLRGQASQFMRAQKKDSKTSSEGKATRIPRNELLDLLFKLFESYEYWSLKGLKERTKQPEVYLKEVLD
DIAILIKKGPYSMKYSLKPEYKKNGLGGNGIADGNGKVNGETDVEDGSAGGNPSNEGNDGDDDEEEDDDDIEMETVV

GO term prediction

Biological Process

GO:0006366 transcription from RNA polymerase II promoter
GO:0006367 transcription initiation from RNA polymerase II promoter

Molecular Function

GO:0003824 catalytic activity

Cellular Component

GO:0005674 transcription factor TFIIF complex

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Entry type

Status

Per-residue features