MCA_02813_1

Protein

Length

379 amino acids

Gene name: TFG2

Description: Transcription initiation factor IIF subunit beta

Browser: contigB:2443909-2445129-

RNA-seq: read pairs 1850, FPKM 60.2, percentile rank 69.4% (100% = highest expression)

Protein function

Annotation:		TFG2	Transcription initiation factor IIF subunit beta
KEGG:	K03139	TFIIF2	transcription initiation factor TFIIF subunit beta [EC:3.6.4.12]
EGGNOG:	0PHE0	PGUG_02088	transcription initiation factor
SGD closest match:	S000003237	TFG2	Transcription initiation factor IIF subunit beta
CGD closest match:	CAL0000197597	orf19.2111	Transcription factor IIF subunit

Protein alignments

	%id	Aln length	E-value
MIA_01792_1	61.38%	290	9e-111	MIA_01792_1
A0A060T2B2_BLAAD	48.79%	289	1e-83	ARAD1A06930p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1A06930g PE=4 SV=1
A0A0J9X9M5_GEOCN	52.43%	288	1e-80	Similar to Saccharomyces cerevisiae YGR005C TFG2 TFIIF (Transcription Factor II) middle subunit OS=Geotrichum candidum GN=BN980_GECA05s02837g PE=4 SV=1
UniRef50_A0A0J9X9M5	52.43%	288	2e-77	Similar to Saccharomyces cerevisiae YGR005C TFG2 TFIIF (Transcription Factor II) middle subunit n=2 Tax=Saccharomycetales TaxID=4892 RepID=A0A0J9X9M5_GEOCN
A0A167C0J4_9ASCO	45.43%	317	1e-80	Transcription factor IIF subunit TFG2 OS=Sugiyamaella lignohabitans GN=TFG2 PE=4 SV=1
A0A1E3PPV0_9ASCO	45.10%	337	3e-79	Uncharacterized protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_45422 PE=4 SV=1
Q6C1J3_YARLI	42.51%	334	8e-71	YALI0F15785p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_F15785g PE=4 SV=1
T2FB_YEAST	40.07%	307	1e-66	Transcription initiation factor IIF subunit beta OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TFG2 PE=1 SV=2
A0A1E4TAM5_9ASCO	39.86%	291	7e-57	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_3446 PE=4 SV=1
A0A1D8PG13_CANAL	38.03%	305	1e-53	Transcription factor IIF subunit OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=orf19.2111 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.1324

Protein family membership

Transcription initiation factor IIF, beta subunit (IPR003196)

Domains and repeats

Domain
44 - 147
Transcription Factor IIF, Rap30/Rap74, interaction (IPR011039)
273 - 339
Winged helix-turn-helix DNA-binding domain (IPR011991)

1 50 100 150 200 250 300 350 379

Detailed signature matches

IPR003196

Transcription initiation factor IIF, beta subunit
1. PF02270 (TFIIF_beta)
  
  49 - 337
  
  Pfam
  PF02270 (TFIIF_beta)
IPR011039

Transcription Factor IIF, Rap30/Rap74, interaction
1. SSF50916 (Rap30/74 ...)
  
  44 - 147
  
  SUPERFAMILY
  SSF50916 (Rap30/74 interaction domains)
IPR011991

Winged helix-turn-helix DNA-binding domain
1. G3DSA:1.10.10.10
  
  273 - 339
  
  GENE3D
  G3DSA:1.10.10.10 (G3DSA:1.10.10.10)
2. SSF46785 ("Winged h...)
  
  274 - 336
  
  SUPERFAMILY
  SSF46785 ("Winged helix" DNA-binding domain)

no IPR

Unintegrated signatures

Coil

144 - 168

COILS
Coil (Coil)
cd07980 (TFIIF_beta)

47 - 203

CDD
cd07980 (TFIIF_beta)
mobidb-lite (disord...)

336 - 379

MobiDB Lite
mobidb-lite (disorder_prediction)

137 - 169

MobiDB Lite
mobidb-lite (disorder_prediction)

1 - 48

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

heterodimer interf...

47S

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

48L

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

54K

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

56G

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

59V

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

60W

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

61L

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

62V

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

63R

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

64L

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

65P

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

66K

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

69M

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

72W

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

75P

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

82E

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

83L

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

84G

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

85H

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

86V

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

87R

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

88I

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

89Y

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

90S

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

103K

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

130D

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

131S

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

132S

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

133T

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

134Y

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

135V

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

136F

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

137T

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

138E

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

139K

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

140D

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

189T

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

191L

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

192V

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

198E

heterodimer interface
47S, 48L, 54K, 56G, 59V, 60W, 61L, 62V, 63R, 64L, 65P, 66K, 69M, 72W, 75P, 82E, 83L, 84G, 85H, 86V, 87R, 88I, 89Y, 90S, 103K, 130D, 131S, 132S, 133T, 134Y, 135V, 136F, 137T, 138E, 139K, 140D, 189T, 191L, 192V, 198E
CDD
cd07980 (TFIIF_beta)

Protein sequence

>MCA_02813_1
MSSKPVIIKKEQQDVKTEPSFSNASGSSQDVKMEVDEDEEEDVFEDSLDLDTSKAGNKVWLVRLPKFLMEKWHNPDNLHG
QELGHVRIYSDDSNLPSDQQKVKVKLILNNSPENSDIPHEYDVSLQKHADSSTYVFTEKDMPKYDKKRAQQEAAKKEQQR
LQQQQQRKGKFQFNKDRFVPYVKTIPKHTALVGKVFNECNVQPSLADPNYFKVLRERRKIQEDEPRPKVTFLEDTTAVAN
EALRGGSASMFMKAQKKEGKASGYSGSAADGKATRLEKEQLLDILFKLFESHPYWTLKGLKERTHQPEQYLKQVLDEIAI
LNKRGPYALTYQLKPENSINNKEGDNATGDDAGDGSDANKSPEDEDDDDDENIELENVA

GO term prediction

Biological Process

GO:0006366 transcription from RNA polymerase II promoter
GO:0006367 transcription initiation from RNA polymerase II promoter

Molecular Function

GO:0003824 catalytic activity

Cellular Component

GO:0005674 transcription factor TFIIF complex

Filter view on

Entry type

Status

Per-residue features