MIA_01779_1

Protein

Length

590 amino acids

Protein function

EGGNOG:	0PUDF		AICARFT_IMPCHas
SGD closest match:	S000004727	ADE17	Bifunctional purine biosynthesis protein ADE17
CGD closest match:	CAL0000191899	ADE17	Bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase

Protein alignments

	%id	Aln length
MCA_02853_1	88.47%	607	MCA_02853_1
A0A0J9XD15_GEOCN	87.10%	589	Similar to Saccharomyces cerevisiae YMR120C ADE17 Enzyme of 'de novo' purine biosynthesis OS=Geotrichum candidum GN=BN980_GECA10s01088g PE=3 SV=1
A0A161HHE3_9ASCO	86.42%	589	Bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase ADE17 OS=Sugiyamaella lignohabitans GN=ADE17 PE=3 SV=1
A0A1E3PDR0_9ASCO	86.32%	585	AICARFT/IMPCHase bienzyme OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_53218 PE=3 SV=1
A0A060T558_BLAAD	84.92%	590	ARAD1B05016p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1B05016g PE=3 SV=1
Q6C7Y2_YARLI	84.92%	590	YALI0D24409p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_D24409g PE=3 SV=1
UniRef50_B9WLL1	84.10%	585	Bifunctional purine biosynthesis protein, [includes phosphoribosylaminoimidazolecarboxamide formyltransferase (Ec 2.1.2.3) IMP cyclohydrolase (Ec 3.5.4.10)], putative n=18 Tax=Opisthokonta TaxID=33154 RepID=B9WLL1_CANDC
Q5A6R2_CANAL	83.76%	585	Bifunctional phosphoribosylaminoimidazolecarboxamide formyltransferase/IMP cyclohydrolase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=ADE17 PE=3 SV=1
PUR92_YEAST	80.57%	592	Bifunctional purine biosynthesis protein ADE17 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ADE17 PE=1 SV=2
A0A1E4TFR5_9ASCO	78.96%	385	Uncharacterized protein (Fragment) OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_31464 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0429

Protein family membership

AICARFT/IMPCHase bienzyme (IPR002695)

Domains and repeats

Domain
3 - 195
Methylglyoxal synthase-like domain (IPR011607)
225 - 370
AICAR transformylase domain (IPR024051)
371 - 505
AICAR transformylase domain (IPR024051)
506 - 580
AICAR transformylase domain (IPR024051)
Domain
201 - 590
Cytidine deaminase-like (IPR016193)

1 100 200 300 400 500 590

Detailed signature matches

IPR002695

AICARFT/IMPCHase bienzyme
1. PF01808 (AICARFT_IM...)
  
  136 - 458
  
  Pfam
  PF01808 (AICARFT_IMPCHas)
2. TIGR00355
  
  6 - 535
  
  TIGRFAMs
  TIGR00355 (TIGR00355)
3. MF_00139 (PurH)
  
  5 - 590
  
  HAMAP
  MF_00139 (PurH)
4. SM00798 (aicarft_im...)
  
  136 - 459
  
  SMART
  SM00798 (aicarft_impchas)
5. PIRSF000414 (PurH)
  
  2 - 590
  
  PIRSF
  PIRSF000414 (PurH)
IPR011607

Methylglyoxal synthase-like domain
1. PF02142 (MGS)
  
  17 - 131
  
  Pfam
  PF02142 (MGS)
2. G3DSA:3.40.50...
  
  3 - 193
  
  GENE3D
  G3DSA:3.40.50.1380 (G3DSA:3.40.50.1380)
3. SSF52335 (Methylgly...)
  
  5 - 195
  
  SUPERFAMILY
  SSF52335 (Methylglyoxal synthase-like)
4. SM00851 (MGS_2a)
  
  17 - 131
  
  SMART
  SM00851 (MGS_2a)
IPR016193

Cytidine deaminase-like
1. SSF53927 (Cytidine ...)
  
  201 - 590
  
  SUPERFAMILY
  SSF53927 (Cytidine deaminase-like)
IPR024051

AICAR transformylase domain
1. G3DSA:3.40.14...
  
  371 - 505
  
  GENE3D
  G3DSA:3.40.140.20 (G3DSA:3.40.140.20)
  
  506 - 580
  
  GENE3D
  G3DSA:3.40.140.20 (G3DSA:3.40.140.20)
  
  225 - 370
  
  GENE3D
  G3DSA:3.40.140.20 (G3DSA:3.40.140.20)

no IPR

Unintegrated signatures

cd01421 (IMPCH)

6 - 191

CDD
cd01421 (IMPCH)

Residue annotation

purine monophospha...

13Y

purine monophosphate binding site
13Y, 15K, 35S, 38T, 67K, 68T, 103N, 126D
CDD
cd01421 (IMPCH)

15K

purine monophosphate binding site
13Y, 15K, 35S, 38T, 67K, 68T, 103N, 126D
CDD
cd01421 (IMPCH)

35S

purine monophosphate binding site
13Y, 15K, 35S, 38T, 67K, 68T, 103N, 126D
CDD
cd01421 (IMPCH)

38T

purine monophosphate binding site
13Y, 15K, 35S, 38T, 67K, 68T, 103N, 126D
CDD
cd01421 (IMPCH)

67K

purine monophosphate binding site
13Y, 15K, 35S, 38T, 67K, 68T, 103N, 126D
CDD
cd01421 (IMPCH)

68T

purine monophosphate binding site
13Y, 15K, 35S, 38T, 67K, 68T, 103N, 126D
CDD
cd01421 (IMPCH)

103N

purine monophosphate binding site
13Y, 15K, 35S, 38T, 67K, 68T, 103N, 126D
CDD
cd01421 (IMPCH)

126D

purine monophosphate binding site
13Y, 15K, 35S, 38T, 67K, 68T, 103N, 126D
CDD
cd01421 (IMPCH)
dimer interface cd...

58A

dimer interface
58A, 61M, 62L, 65R, 66V, 69L, 70H, 71P, 74H, 75G, 78L, 125I, 126D, 127I, 130V, 134R, 135A, 138K, 170N, 173A, 174L, 175K, 183Y, 184D, 185A, 187I, 188A, 191F
CDD
cd01421 (IMPCH)

61M

dimer interface
58A, 61M, 62L, 65R, 66V, 69L, 70H, 71P, 74H, 75G, 78L, 125I, 126D, 127I, 130V, 134R, 135A, 138K, 170N, 173A, 174L, 175K, 183Y, 184D, 185A, 187I, 188A, 191F
CDD
cd01421 (IMPCH)

62L

dimer interface
58A, 61M, 62L, 65R, 66V, 69L, 70H, 71P, 74H, 75G, 78L, 125I, 126D, 127I, 130V, 134R, 135A, 138K, 170N, 173A, 174L, 175K, 183Y, 184D, 185A, 187I, 188A, 191F
CDD
cd01421 (IMPCH)

65R

dimer interface
58A, 61M, 62L, 65R, 66V, 69L, 70H, 71P, 74H, 75G, 78L, 125I, 126D, 127I, 130V, 134R, 135A, 138K, 170N, 173A, 174L, 175K, 183Y, 184D, 185A, 187I, 188A, 191F
CDD
cd01421 (IMPCH)

66V

dimer interface
58A, 61M, 62L, 65R, 66V, 69L, 70H, 71P, 74H, 75G, 78L, 125I, 126D, 127I, 130V, 134R, 135A, 138K, 170N, 173A, 174L, 175K, 183Y, 184D, 185A, 187I, 188A, 191F
CDD
cd01421 (IMPCH)

69L

dimer interface
58A, 61M, 62L, 65R, 66V, 69L, 70H, 71P, 74H, 75G, 78L, 125I, 126D, 127I, 130V, 134R, 135A, 138K, 170N, 173A, 174L, 175K, 183Y, 184D, 185A, 187I, 188A, 191F
CDD
cd01421 (IMPCH)

70H

dimer interface
58A, 61M, 62L, 65R, 66V, 69L, 70H, 71P, 74H, 75G, 78L, 125I, 126D, 127I, 130V, 134R, 135A, 138K, 170N, 173A, 174L, 175K, 183Y, 184D, 185A, 187I, 188A, 191F
CDD
cd01421 (IMPCH)

71P

dimer interface
58A, 61M, 62L, 65R, 66V, 69L, 70H, 71P, 74H, 75G, 78L, 125I, 126D, 127I, 130V, 134R, 135A, 138K, 170N, 173A, 174L, 175K, 183Y, 184D, 185A, 187I, 188A, 191F
CDD
cd01421 (IMPCH)

74H

dimer interface
58A, 61M, 62L, 65R, 66V, 69L, 70H, 71P, 74H, 75G, 78L, 125I, 126D, 127I, 130V, 134R, 135A, 138K, 170N, 173A, 174L, 175K, 183Y, 184D, 185A, 187I, 188A, 191F
CDD
cd01421 (IMPCH)

75G

dimer interface
58A, 61M, 62L, 65R, 66V, 69L, 70H, 71P, 74H, 75G, 78L, 125I, 126D, 127I, 130V, 134R, 135A, 138K, 170N, 173A, 174L, 175K, 183Y, 184D, 185A, 187I, 188A, 191F
CDD
cd01421 (IMPCH)

78L

dimer interface
58A, 61M, 62L, 65R, 66V, 69L, 70H, 71P, 74H, 75G, 78L, 125I, 126D, 127I, 130V, 134R, 135A, 138K, 170N, 173A, 174L, 175K, 183Y, 184D, 185A, 187I, 188A, 191F
CDD
cd01421 (IMPCH)

125I

dimer interface
58A, 61M, 62L, 65R, 66V, 69L, 70H, 71P, 74H, 75G, 78L, 125I, 126D, 127I, 130V, 134R, 135A, 138K, 170N, 173A, 174L, 175K, 183Y, 184D, 185A, 187I, 188A, 191F
CDD
cd01421 (IMPCH)

126D

dimer interface
58A, 61M, 62L, 65R, 66V, 69L, 70H, 71P, 74H, 75G, 78L, 125I, 126D, 127I, 130V, 134R, 135A, 138K, 170N, 173A, 174L, 175K, 183Y, 184D, 185A, 187I, 188A, 191F
CDD
cd01421 (IMPCH)

127I

dimer interface
58A, 61M, 62L, 65R, 66V, 69L, 70H, 71P, 74H, 75G, 78L, 125I, 126D, 127I, 130V, 134R, 135A, 138K, 170N, 173A, 174L, 175K, 183Y, 184D, 185A, 187I, 188A, 191F
CDD
cd01421 (IMPCH)

130V

dimer interface
58A, 61M, 62L, 65R, 66V, 69L, 70H, 71P, 74H, 75G, 78L, 125I, 126D, 127I, 130V, 134R, 135A, 138K, 170N, 173A, 174L, 175K, 183Y, 184D, 185A, 187I, 188A, 191F
CDD
cd01421 (IMPCH)

134R

dimer interface
58A, 61M, 62L, 65R, 66V, 69L, 70H, 71P, 74H, 75G, 78L, 125I, 126D, 127I, 130V, 134R, 135A, 138K, 170N, 173A, 174L, 175K, 183Y, 184D, 185A, 187I, 188A, 191F
CDD
cd01421 (IMPCH)

135A

dimer interface
58A, 61M, 62L, 65R, 66V, 69L, 70H, 71P, 74H, 75G, 78L, 125I, 126D, 127I, 130V, 134R, 135A, 138K, 170N, 173A, 174L, 175K, 183Y, 184D, 185A, 187I, 188A, 191F
CDD
cd01421 (IMPCH)

138K

dimer interface
58A, 61M, 62L, 65R, 66V, 69L, 70H, 71P, 74H, 75G, 78L, 125I, 126D, 127I, 130V, 134R, 135A, 138K, 170N, 173A, 174L, 175K, 183Y, 184D, 185A, 187I, 188A, 191F
CDD
cd01421 (IMPCH)

170N

dimer interface
58A, 61M, 62L, 65R, 66V, 69L, 70H, 71P, 74H, 75G, 78L, 125I, 126D, 127I, 130V, 134R, 135A, 138K, 170N, 173A, 174L, 175K, 183Y, 184D, 185A, 187I, 188A, 191F
CDD
cd01421 (IMPCH)

173A

dimer interface
58A, 61M, 62L, 65R, 66V, 69L, 70H, 71P, 74H, 75G, 78L, 125I, 126D, 127I, 130V, 134R, 135A, 138K, 170N, 173A, 174L, 175K, 183Y, 184D, 185A, 187I, 188A, 191F
CDD
cd01421 (IMPCH)

174L

dimer interface
58A, 61M, 62L, 65R, 66V, 69L, 70H, 71P, 74H, 75G, 78L, 125I, 126D, 127I, 130V, 134R, 135A, 138K, 170N, 173A, 174L, 175K, 183Y, 184D, 185A, 187I, 188A, 191F
CDD
cd01421 (IMPCH)

175K

dimer interface
58A, 61M, 62L, 65R, 66V, 69L, 70H, 71P, 74H, 75G, 78L, 125I, 126D, 127I, 130V, 134R, 135A, 138K, 170N, 173A, 174L, 175K, 183Y, 184D, 185A, 187I, 188A, 191F
CDD
cd01421 (IMPCH)

183Y

dimer interface
58A, 61M, 62L, 65R, 66V, 69L, 70H, 71P, 74H, 75G, 78L, 125I, 126D, 127I, 130V, 134R, 135A, 138K, 170N, 173A, 174L, 175K, 183Y, 184D, 185A, 187I, 188A, 191F
CDD
cd01421 (IMPCH)

184D

dimer interface
58A, 61M, 62L, 65R, 66V, 69L, 70H, 71P, 74H, 75G, 78L, 125I, 126D, 127I, 130V, 134R, 135A, 138K, 170N, 173A, 174L, 175K, 183Y, 184D, 185A, 187I, 188A, 191F
CDD
cd01421 (IMPCH)

185A

dimer interface
58A, 61M, 62L, 65R, 66V, 69L, 70H, 71P, 74H, 75G, 78L, 125I, 126D, 127I, 130V, 134R, 135A, 138K, 170N, 173A, 174L, 175K, 183Y, 184D, 185A, 187I, 188A, 191F
CDD
cd01421 (IMPCH)

187I

dimer interface
58A, 61M, 62L, 65R, 66V, 69L, 70H, 71P, 74H, 75G, 78L, 125I, 126D, 127I, 130V, 134R, 135A, 138K, 170N, 173A, 174L, 175K, 183Y, 184D, 185A, 187I, 188A, 191F
CDD
cd01421 (IMPCH)

188A

dimer interface
58A, 61M, 62L, 65R, 66V, 69L, 70H, 71P, 74H, 75G, 78L, 125I, 126D, 127I, 130V, 134R, 135A, 138K, 170N, 173A, 174L, 175K, 183Y, 184D, 185A, 187I, 188A, 191F
CDD
cd01421 (IMPCH)

191F

dimer interface
58A, 61M, 62L, 65R, 66V, 69L, 70H, 71P, 74H, 75G, 78L, 125I, 126D, 127I, 130V, 134R, 135A, 138K, 170N, 173A, 174L, 175K, 183Y, 184D, 185A, 187I, 188A, 191F
CDD
cd01421 (IMPCH)
putative catalytic...

67K

putative catalytic residues
67K, 126D
CDD
cd01421 (IMPCH)

126D

putative catalytic residues
67K, 126D
CDD
cd01421 (IMPCH)

Protein sequence

>MIA_01779_1
MPGQQKTAILSVYDKTGLLDLAKGLVDNGVRILASGGTAKMVRGAGFPVEDVSSITHAPEMLGGRVKTLHPAVHGGILAR
NIESDEKDLEAQNIDKVDFVVCNLYPFKETVSKVNVTIDEAVEEIDIGGVTLLRAAAKNHSRVTILSDPSDYHIFIQELS
QGGVSAETRNRLALKAFEHTAEYDATIADFFRKQYSEDITYLPLRYGANPHQKPAQAFVTKGELPFKVLSGSPGYINLLD
ALNSWPLVKELSAALSLPAAASFKHVSPAGAAVGLPLSDVEKKLYFVSDIENLSPLANAYARARGADRMSSFGDWIALSN
IVDLPTAQIISKEVSDGVIAPGYEPAALEILKKKKGGKYCVLQIDPNYNPDLIETRQVYGVSLQQKRNDAIINASTFKEI
VSDKKDLPEQAIIDLTVATIALKYTQSNSVCYAKNGMVIGLGAGQQSRIHCTRLAGDKADNWWLRQHPRVLAFKWAPGTK
RPDKSNAIDLYVSNQIPTEGPEKVEYESKFAEIPTPLTAEERKEWISKNTGVALASDAFFPFPDNVYRAVRSGASYISAP
FGSVQDKAVLAAANSFDLVYVENNIRLFHH

GO term prediction

Biological Process

GO:0006164 purine nucleotide biosynthetic process

Molecular Function

GO:0003824 catalytic activity
GO:0003937 IMP cyclohydrolase activity
GO:0004643 phosphoribosylaminoimidazolecarboxamide formyltransferase activity

Cellular Component

None predicted.

Filter view on

Entry type

Status

Per-residue features