MIA_01774_1

Protein

Length

1,339 amino acids

Protein function

EGGNOG:	0PFXN	IRE1	protein kinase and ribonuclease Ire1
SGD closest match:	S000001121	IRE1	Serine/threonine-protein kinase/endoribonuclease IRE1
CGD closest match:	CAL0000181249	IRE1	Bifunctional endoribonuclease/protein kinase

Protein alignments

	%id	Aln length	E-value
MCA_03350_1	51.913%	1281	0.0	MCA_03350_1
A0A0J9XES8_GEOCN	59.590%	537	0.0	Similar to Saccharomyces cerevisiae YHR079C IRE1 Serine-threonine kinase and endoribonuclease OS=Geotrichum candidum GN=BN980_GECA12s02573g PE=4 SV=1
UniRef50_A0A0J9XES8	59.590%	537	0.0	Similar to Saccharomyces cerevisiae YHR079C IRE1 Serine-threonine kinase and endoribonuclease n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9XES8_GEOCN
A0A1E3PDA1_9ASCO	58.101%	537	2.15e-180	Uncharacterized protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_84511 PE=4 SV=1
A0A167FU59_9ASCO	51.407%	533	4.57e-151	Bifunctional endoribonuclease/protein kinase IRE1 OS=Sugiyamaella lignohabitans GN=IRE1 PE=4 SV=1
W0TYQ2_YARLI	50.187%	536	2.02e-144	YALI0A14839p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_A14839g PE=4 SV=1
A0A1D8PE76_CANAL	47.114%	537	3.08e-132	Bifunctional endoribonuclease/protein kinase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=IRE1 PE=4 SV=1
A0A060T7Y2_BLAAD	46.780%	528	1.13e-125	ARAD1C31724p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C31724g PE=4 SV=1
A0A1E4TCS0_9ASCO	44.757%	534	8.16e-126	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_140799 PE=4 SV=1
IRE1_YEAST	44.612%	529	4.13e-123	Serine/threonine-protein kinase/endoribonuclease IRE1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=IRE1 PE=1 SV=2

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.5648
Predicted cleavage: 124

Protein family membership

None predicted.

Domains and repeats

Domain
117 - 258
Quinoprotein alcohol dehydrogenase-like superfamily (IPR011047)
291 - 351
Quinoprotein alcohol dehydrogenase-like superfamily (IPR011047)
808 - 1,209
Protein kinase-like domain (IPR011009)
Protein kinase domain (IPR000719)
1,267 - 1,321
PUB domain (IPR018997)
Domain
1,205 - 1,336
KEN domain (IPR010513)
Repeat
118 - 149
Pyrrolo-quinoline quinone beta-propeller repeat (IPR018391)
213 - 244
Pyrrolo-quinoline quinone beta-propeller repeat (IPR018391)

1 200 400 600 800 1000 1200 1339

Detailed signature matches

IPR011047

Quinoprotein alcohol dehydrogenase-like superfamily
1. SSF50998 (Quinoprot...)
  
  117 - 258
  
  SUPERFAMILY
  SSF50998 (Quinoprotein alcohol dehydrogenase-like)
  
  291 - 351
  
  SUPERFAMILY
  SSF50998 (Quinoprotein alcohol dehydrogenase-like)
IPR011009

Protein kinase-like domain
1. SSF56112 (Protein k...)
  
  1,096 - 1,209
  
  SUPERFAMILY
  SSF56112 (Protein kinase-like (PK-like))
  
  925 - 967
  
  SUPERFAMILY
  SSF56112 (Protein kinase-like (PK-like))
  
  811 - 891
  
  SUPERFAMILY
  SSF56112 (Protein kinase-like (PK-like))
  
  1,000 - 1,042
  
  SUPERFAMILY
  SSF56112 (Protein kinase-like (PK-like))
IPR000719

Protein kinase domain
1. PS50011 (PROTEIN_KI...)
  
  808 - 1,202
  
  PROSITE profiles
  PS50011 (PROTEIN_KINASE_DOM)
2. SM00220 (serkin_6)
  
  808 - 1,202
  
  SMART
  SM00220 (serkin_6)
3. PF00069 (Pkinase)
  
  1,095 - 1,202
  
  Pfam
  PF00069 (Pkinase)
  
  811 - 1,042
  
  Pfam
  PF00069 (Pkinase)
IPR010513

KEN domain
1. PS51392 (KEN)
  
  1,205 - 1,336
  
  PROSITE profiles
  PS51392 (KEN)
2. PF06479 (Ribonuc_2-5A)
  
  1,208 - 1,334
  
  Pfam
  PF06479 (Ribonuc_2-5A)
IPR018997

PUB domain
1. SM00580 (PGNneu)
  
  1,267 - 1,321
  
  SMART
  SM00580 (PGNneu)
IPR018391

Pyrrolo-quinoline quinone beta-propeller repeat
1. SM00564 (ire1_9)
  
  213 - 244
  
  SMART
  SM00564 (ire1_9)
  
  118 - 149
  
  SMART
  SM00564 (ire1_9)
IPR008271

Serine/threonine-protein kinase, active site
1. PS00108 (PROTEIN_KI...)
  
  955 - 967
  
  PROSITE patterns
  PS00108 (PROTEIN_KINASE_ST)

no IPR

Unintegrated signatures

CYTOPLASMIC_D... (C...)

1 - 562

PHOBIUS
CYTOPLASMIC_DOMAIN (Cytoplasmic domain)
Coil

1,339 - 1,339

COILS
Coil (Coil)
G3DSA:1.10.51...

946 - 1,211

GENE3D
G3DSA:1.10.510.10 (G3DSA:1.10.510.10)
G3DSA:2.140.1...

44 - 759

GENE3D
G3DSA:2.140.10.10 (G3DSA:2.140.10.10)
G3DSA:3.30.20...

798 - 945

GENE3D
G3DSA:3.30.200.20 (G3DSA:3.30.200.20)
NON_CYTOPLASM... (N...)

583 - 1,339

PHOBIUS
NON_CYTOPLASMIC_DOMAIN (Non cytoplasmic domain)
TMhelix

563 - 585

TMHMM
TMhelix (TMhelix)
TRANSMEMBRANE (Tran...)

563 - 582

PHOBIUS
TRANSMEMBRANE (Transmembrane region)
cd10422 (RNase_Ire1)

1,206 - 1,334

CDD
cd10422 (RNase_Ire1)
mobidb-lite (disord...)

782 - 801

MobiDB Lite
mobidb-lite (disorder_prediction)

969 - 993

MobiDB Lite
mobidb-lite (disorder_prediction)

634 - 757

MobiDB Lite
mobidb-lite (disorder_prediction)

897 - 927

MobiDB Lite
mobidb-lite (disorder_prediction)

506 - 544

MobiDB Lite
mobidb-lite (disorder_prediction)

1,062 - 1,090

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

ligand binding sit...

1,206S

ligand binding site
1,206S, 1,207K, 1,210D, 1,214K, 1,280S, 1,298P, 1,329V, 1,332P, 1,333F
CDD
cd10422 (RNase_Ire1)

1,207K

ligand binding site
1,206S, 1,207K, 1,210D, 1,214K, 1,280S, 1,298P, 1,329V, 1,332P, 1,333F
CDD
cd10422 (RNase_Ire1)

1,210D

ligand binding site
1,206S, 1,207K, 1,210D, 1,214K, 1,280S, 1,298P, 1,329V, 1,332P, 1,333F
CDD
cd10422 (RNase_Ire1)

1,214K

ligand binding site
1,206S, 1,207K, 1,210D, 1,214K, 1,280S, 1,298P, 1,329V, 1,332P, 1,333F
CDD
cd10422 (RNase_Ire1)

1,280S

ligand binding site
1,206S, 1,207K, 1,210D, 1,214K, 1,280S, 1,298P, 1,329V, 1,332P, 1,333F
CDD
cd10422 (RNase_Ire1)

1,298P

ligand binding site
1,206S, 1,207K, 1,210D, 1,214K, 1,280S, 1,298P, 1,329V, 1,332P, 1,333F
CDD
cd10422 (RNase_Ire1)

1,329V

ligand binding site
1,206S, 1,207K, 1,210D, 1,214K, 1,280S, 1,298P, 1,329V, 1,332P, 1,333F
CDD
cd10422 (RNase_Ire1)

1,332P

ligand binding site
1,206S, 1,207K, 1,210D, 1,214K, 1,280S, 1,298P, 1,329V, 1,332P, 1,333F
CDD
cd10422 (RNase_Ire1)

1,333F

ligand binding site
1,206S, 1,207K, 1,210D, 1,214K, 1,280S, 1,298P, 1,329V, 1,332P, 1,333F
CDD
cd10422 (RNase_Ire1)
kinase interface c...

1,208R

kinase interface
1,208R, 1,306L, 1,310P, 1,313L, 1,314M, 1,318E
CDD
cd10422 (RNase_Ire1)

1,306L

kinase interface
1,208R, 1,306L, 1,310P, 1,313L, 1,314M, 1,318E
CDD
cd10422 (RNase_Ire1)

1,310P

kinase interface
1,208R, 1,306L, 1,310P, 1,313L, 1,314M, 1,318E
CDD
cd10422 (RNase_Ire1)

1,313L

kinase interface
1,208R, 1,306L, 1,310P, 1,313L, 1,314M, 1,318E
CDD
cd10422 (RNase_Ire1)

1,314M

kinase interface
1,208R, 1,306L, 1,310P, 1,313L, 1,314M, 1,318E
CDD
cd10422 (RNase_Ire1)

1,318E

kinase interface
1,208R, 1,306L, 1,310P, 1,313L, 1,314M, 1,318E
CDD
cd10422 (RNase_Ire1)
homodimer interfac...

1,210D

homodimer interface
1,210D, 1,214K, 1,217D, 1,218R, 1,277R, 1,280S, 1,281H, 1,284N, 1,297Y, 1,298P, 1,299E, 1,328D, 1,329V, 1,332P
CDD
cd10422 (RNase_Ire1)

1,214K

homodimer interface
1,210D, 1,214K, 1,217D, 1,218R, 1,277R, 1,280S, 1,281H, 1,284N, 1,297Y, 1,298P, 1,299E, 1,328D, 1,329V, 1,332P
CDD
cd10422 (RNase_Ire1)

1,217D

homodimer interface
1,210D, 1,214K, 1,217D, 1,218R, 1,277R, 1,280S, 1,281H, 1,284N, 1,297Y, 1,298P, 1,299E, 1,328D, 1,329V, 1,332P
CDD
cd10422 (RNase_Ire1)

1,218R

homodimer interface
1,210D, 1,214K, 1,217D, 1,218R, 1,277R, 1,280S, 1,281H, 1,284N, 1,297Y, 1,298P, 1,299E, 1,328D, 1,329V, 1,332P
CDD
cd10422 (RNase_Ire1)

1,277R

homodimer interface
1,210D, 1,214K, 1,217D, 1,218R, 1,277R, 1,280S, 1,281H, 1,284N, 1,297Y, 1,298P, 1,299E, 1,328D, 1,329V, 1,332P
CDD
cd10422 (RNase_Ire1)

1,280S

homodimer interface
1,210D, 1,214K, 1,217D, 1,218R, 1,277R, 1,280S, 1,281H, 1,284N, 1,297Y, 1,298P, 1,299E, 1,328D, 1,329V, 1,332P
CDD
cd10422 (RNase_Ire1)

1,281H

homodimer interface
1,210D, 1,214K, 1,217D, 1,218R, 1,277R, 1,280S, 1,281H, 1,284N, 1,297Y, 1,298P, 1,299E, 1,328D, 1,329V, 1,332P
CDD
cd10422 (RNase_Ire1)

1,284N

homodimer interface
1,210D, 1,214K, 1,217D, 1,218R, 1,277R, 1,280S, 1,281H, 1,284N, 1,297Y, 1,298P, 1,299E, 1,328D, 1,329V, 1,332P
CDD
cd10422 (RNase_Ire1)

1,297Y

homodimer interface
1,210D, 1,214K, 1,217D, 1,218R, 1,277R, 1,280S, 1,281H, 1,284N, 1,297Y, 1,298P, 1,299E, 1,328D, 1,329V, 1,332P
CDD
cd10422 (RNase_Ire1)

1,298P

homodimer interface
1,210D, 1,214K, 1,217D, 1,218R, 1,277R, 1,280S, 1,281H, 1,284N, 1,297Y, 1,298P, 1,299E, 1,328D, 1,329V, 1,332P
CDD
cd10422 (RNase_Ire1)

1,299E

homodimer interface
1,210D, 1,214K, 1,217D, 1,218R, 1,277R, 1,280S, 1,281H, 1,284N, 1,297Y, 1,298P, 1,299E, 1,328D, 1,329V, 1,332P
CDD
cd10422 (RNase_Ire1)

1,328D

homodimer interface
1,210D, 1,214K, 1,217D, 1,218R, 1,277R, 1,280S, 1,281H, 1,284N, 1,297Y, 1,298P, 1,299E, 1,328D, 1,329V, 1,332P
CDD
cd10422 (RNase_Ire1)

1,329V

homodimer interface
1,210D, 1,214K, 1,217D, 1,218R, 1,277R, 1,280S, 1,281H, 1,284N, 1,297Y, 1,298P, 1,299E, 1,328D, 1,329V, 1,332P
CDD
cd10422 (RNase_Ire1)

1,332P

homodimer interface
1,210D, 1,214K, 1,217D, 1,218R, 1,277R, 1,280S, 1,281H, 1,284N, 1,297Y, 1,298P, 1,299E, 1,328D, 1,329V, 1,332P
CDD
cd10422 (RNase_Ire1)

Protein sequence

>MIA_01774_1
MSRIKWIRRRHPVLPEAGACPSGPAARGSLFFTMLLRYITLFLLLTTTLARPPDAGRQHQQRMNGYQTTMSVNNNRHKTP
PNNLPASILHHSAISQNALAAVSSGGVSSLISPRSIADFEVSDFILVCSIDGSLHARDRQTGLEIWSIPGERPLAQVSSG
SHSSCNNGECPERDLIWIVEPLGEGNLFYFTPELGLQQLPISIKDLVLQSPFALHGDDKIYTGSRHTTLFSIDSATGKVL
KVYGSGKSDLGNAECKLKNPLSHIFRKENTEDTDYFGDEEYDDEDDEEEDYEFLQFSADKGSFLIGRTDYMLEIHGKNET
IWNVTYSTWGPNSVDYDLVAQHYRSPDNIYVTPIYNSSILALSTDGRKKPAQWVGQISTVAISVFDVFRPTKHNPNNQED
QDSLIILPQPLHYKNMQIDSKILSSIYVQKTADGQWFAMSGNNYPSLVHSAPLAPWCSENFNSYGQDITSAIIGVHSLNT
KGQQQQQKQQNDDGHQDILAIDGLETEPPLKKRKRTPHSDILKSSPMALPAPFPPYQQDHPGTTPPVAESKLFNSIVRIM
ENFFAFVIFFLVLAVATKIGWFPQLLHIFEVIKATNDSLKSQRTQVTLDDEEEEEMEEKDYSLREKVKFVGISEVSTGDE
ESGAEEASKDLIGVTPASEEVTNAHEGDKTPTVTTPIVKHVEIVEPDRSWREGSDLAEQPDSSLSATGGGIAGGPQSSPG
AGTHKRRKRGSRGGRNNKNKKKNLQSQEAEEGSSDVFDLTPTASSVAISEMAATASSAVLSSPLVSSTPSTPSSPSKNVL
PIQPSQLSVSENVLGYGSHGTVVLKGEFENREVAVKRMLLEFYDVASHEVSLLQESDDHPNVIRYYCKQQSERFLYIALE
LCPGTLEDMIEKRFMSTPRIEYQTPEDERGRGGDGDASLQEQQQQQMSELASIMTPVQIMYQIASGVQHLHSLKIVHRDL
KPQNILVAPPRLKRGGNGGSGSSTAGSGSVGGGLNKGAIGGPVRMLISDFGLCKRLEGDQSSFRTTTAQAAGTSGWRAPE
LLIDDVEGIIYGGNSNTFNIGGSSKGKDGSEGNESALSSSQHNRSSSEPIVIDTLSNRRATRAIDIFSLGCVFYYVLSKG
QHPFGDKILREANIVQNSYSLDYLDSKIAARCKGESYDEEDDEREDDDFEDAVEARDLISRMISRNPKARPDANMVLQHP
LFWSLSKRLDFLLKVSDRFEGESRDPPSALLLEIERDAGRVVGRDWHARLGPEFVENLGKYRKYHGDRVSDLLRAMRNKS
HHFNDLPAGLRRRMGPYPEGYVRFFLRRFPFLLMAVYEMVKRNLAEEDVFKPFFTGVVV

GO term prediction

Biological Process

GO:0006397 mRNA processing
GO:0006468 protein phosphorylation

Molecular Function

GO:0004540 ribonuclease activity
GO:0004672 protein kinase activity
GO:0005524 ATP binding

Cellular Component