MIA_01651_1

Protein

Length

480 amino acids

Protein function

EGGNOG:	0PJTX	FG09240.1	2-oxoisovalerate dehydrogenase
SGD closest match:	S000000980	PDA1	Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial
CGD closest match:	CAL0000181135	PDA1	Pyruvate dehydrogenase E1 component subunit alpha

Protein alignments

	%id	Aln length	E-value
MCA_00309_1	76.499%	417	0.0	MCA_00309_1
A0A0J9X4Z5_GEOCN	68.900%	418	0.0	Uncharacterized protein OS=Geotrichum candidum GN=BN980_GECA02s07358g PE=4 SV=1
A0A161HMV3_9ASCO	65.403%	422	0.0	Pyruvate dehydrogenase (Acetyl-transferring) subunit E1 alpha OS=Sugiyamaella lignohabitans GN=PDA1 PE=4 SV=1
Q6C9S7_YARLI	61.905%	420	0.0	YALI0D08690p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_D08690g PE=4 SV=1
A0A060T960_BLAAD	58.513%	417	0.0	ARAD1D09064p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D09064g PE=4 SV=1
A0A1E4TAP4_9ASCO	58.824%	425	2.69e-176	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_128782 PE=4 SV=1
UniRef50_I1RY12	56.867%	415	1.94e-164	Uncharacterized protein n=42 Tax=saccharomyceta TaxID=716545 RepID=I1RY12_GIBZE
A0A1E3PJ57_9ASCO	51.923%	416	2.82e-147	Putative 2-oxoisovalerate dehydrogenase alpha subunit OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_74860 PE=4 SV=1
Q5A0Z9_CANAL	26.000%	300	4.65e-27	Pyruvate dehydrogenase E1 component subunit alpha OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=PDA1 PE=4 SV=1
ODPA_YEAST	25.501%	349	2.24e-25	Pyruvate dehydrogenase E1 component subunit alpha, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PDA1 PE=1 SV=2

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.9841
Predicted cleavage: 41

Protein family membership

None predicted.

Domains and repeats

Domain
89 - 466
Thiamin diphosphate-binding fold (IPR029061)
Domain
120 - 431
Dehydrogenase, E1 component (IPR001017)

1 50 100 150 200 250 300 350 400 450 480

Detailed signature matches

IPR029061

Thiamin diphosphate-binding fold
1. SSF52518 (Thiamin d...)
  
  89 - 466
  
  SUPERFAMILY
  SSF52518 (Thiamin diphosphate-binding fold (THDP-binding))
IPR001017

Dehydrogenase, E1 component
1. PF00676 (E1_dh)
  
  120 - 431
  
  Pfam
  PF00676 (E1_dh)

no IPR

Unintegrated signatures

G3DSA:3.40.50...

59 - 472

GENE3D
G3DSA:3.40.50.970 (G3DSA:3.40.50.970)
cd02000 (TPP_E1_PDC...)

118 - 421

CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)
mobidb-lite (disord...)

44 - 63

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

tetramer interface...

142S

tetramer interface
142S, 193A, 220S, 221P, 266G, 268A, 269S, 270E, 271G, 274H, 278N, 282T, 297A, 298I, 299S, 300T, 314R, 319G, 367D, 371A
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

193A

tetramer interface
142S, 193A, 220S, 221P, 266G, 268A, 269S, 270E, 271G, 274H, 278N, 282T, 297A, 298I, 299S, 300T, 314R, 319G, 367D, 371A
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

220S

tetramer interface
142S, 193A, 220S, 221P, 266G, 268A, 269S, 270E, 271G, 274H, 278N, 282T, 297A, 298I, 299S, 300T, 314R, 319G, 367D, 371A
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

221P

tetramer interface
142S, 193A, 220S, 221P, 266G, 268A, 269S, 270E, 271G, 274H, 278N, 282T, 297A, 298I, 299S, 300T, 314R, 319G, 367D, 371A
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

266G

tetramer interface
142S, 193A, 220S, 221P, 266G, 268A, 269S, 270E, 271G, 274H, 278N, 282T, 297A, 298I, 299S, 300T, 314R, 319G, 367D, 371A
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

268A

tetramer interface
142S, 193A, 220S, 221P, 266G, 268A, 269S, 270E, 271G, 274H, 278N, 282T, 297A, 298I, 299S, 300T, 314R, 319G, 367D, 371A
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

269S

tetramer interface
142S, 193A, 220S, 221P, 266G, 268A, 269S, 270E, 271G, 274H, 278N, 282T, 297A, 298I, 299S, 300T, 314R, 319G, 367D, 371A
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

270E

tetramer interface
142S, 193A, 220S, 221P, 266G, 268A, 269S, 270E, 271G, 274H, 278N, 282T, 297A, 298I, 299S, 300T, 314R, 319G, 367D, 371A
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

271G

tetramer interface
142S, 193A, 220S, 221P, 266G, 268A, 269S, 270E, 271G, 274H, 278N, 282T, 297A, 298I, 299S, 300T, 314R, 319G, 367D, 371A
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

274H

tetramer interface
142S, 193A, 220S, 221P, 266G, 268A, 269S, 270E, 271G, 274H, 278N, 282T, 297A, 298I, 299S, 300T, 314R, 319G, 367D, 371A
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

278N

tetramer interface
142S, 193A, 220S, 221P, 266G, 268A, 269S, 270E, 271G, 274H, 278N, 282T, 297A, 298I, 299S, 300T, 314R, 319G, 367D, 371A
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

282T

tetramer interface
142S, 193A, 220S, 221P, 266G, 268A, 269S, 270E, 271G, 274H, 278N, 282T, 297A, 298I, 299S, 300T, 314R, 319G, 367D, 371A
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

297A

tetramer interface
142S, 193A, 220S, 221P, 266G, 268A, 269S, 270E, 271G, 274H, 278N, 282T, 297A, 298I, 299S, 300T, 314R, 319G, 367D, 371A
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

298I

tetramer interface
142S, 193A, 220S, 221P, 266G, 268A, 269S, 270E, 271G, 274H, 278N, 282T, 297A, 298I, 299S, 300T, 314R, 319G, 367D, 371A
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

299S

tetramer interface
142S, 193A, 220S, 221P, 266G, 268A, 269S, 270E, 271G, 274H, 278N, 282T, 297A, 298I, 299S, 300T, 314R, 319G, 367D, 371A
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

300T

tetramer interface
142S, 193A, 220S, 221P, 266G, 268A, 269S, 270E, 271G, 274H, 278N, 282T, 297A, 298I, 299S, 300T, 314R, 319G, 367D, 371A
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

314R

tetramer interface
142S, 193A, 220S, 221P, 266G, 268A, 269S, 270E, 271G, 274H, 278N, 282T, 297A, 298I, 299S, 300T, 314R, 319G, 367D, 371A
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

319G

tetramer interface
142S, 193A, 220S, 221P, 266G, 268A, 269S, 270E, 271G, 274H, 278N, 282T, 297A, 298I, 299S, 300T, 314R, 319G, 367D, 371A
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

367D

tetramer interface
142S, 193A, 220S, 221P, 266G, 268A, 269S, 270E, 271G, 274H, 278N, 282T, 297A, 298I, 299S, 300T, 314R, 319G, 367D, 371A
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

371A

tetramer interface
142S, 193A, 220S, 221P, 266G, 268A, 269S, 270E, 271G, 274H, 278N, 282T, 297A, 298I, 299S, 300T, 314R, 319G, 367D, 371A
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)
TPP-binding site c...

171Y

TPP-binding site
171Y, 172R, 220S, 222L, 264G, 265D, 266G, 267A, 294N, 296Y, 363H
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

172R

TPP-binding site
171Y, 172R, 220S, 222L, 264G, 265D, 266G, 267A, 294N, 296Y, 363H
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

220S

TPP-binding site
171Y, 172R, 220S, 222L, 264G, 265D, 266G, 267A, 294N, 296Y, 363H
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

222L

TPP-binding site
171Y, 172R, 220S, 222L, 264G, 265D, 266G, 267A, 294N, 296Y, 363H
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

264G

TPP-binding site
171Y, 172R, 220S, 222L, 264G, 265D, 266G, 267A, 294N, 296Y, 363H
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

265D

TPP-binding site
171Y, 172R, 220S, 222L, 264G, 265D, 266G, 267A, 294N, 296Y, 363H
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

266G

TPP-binding site
171Y, 172R, 220S, 222L, 264G, 265D, 266G, 267A, 294N, 296Y, 363H
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

267A

TPP-binding site
171Y, 172R, 220S, 222L, 264G, 265D, 266G, 267A, 294N, 296Y, 363H
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

294N

TPP-binding site
171Y, 172R, 220S, 222L, 264G, 265D, 266G, 267A, 294N, 296Y, 363H
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

296Y

TPP-binding site
171Y, 172R, 220S, 222L, 264G, 265D, 266G, 267A, 294N, 296Y, 363H
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

363H

TPP-binding site
171Y, 172R, 220S, 222L, 264G, 265D, 266G, 267A, 294N, 296Y, 363H
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)
heterodimer interf...

215I

heterodimer interface
215I, 217P, 219S, 224T, 227P, 228H, 231G, 232A, 234Y, 235A, 238V, 271G, 272D, 278N, 279M, 282T
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

217P

heterodimer interface
215I, 217P, 219S, 224T, 227P, 228H, 231G, 232A, 234Y, 235A, 238V, 271G, 272D, 278N, 279M, 282T
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

219S

heterodimer interface
215I, 217P, 219S, 224T, 227P, 228H, 231G, 232A, 234Y, 235A, 238V, 271G, 272D, 278N, 279M, 282T
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

224T

heterodimer interface
215I, 217P, 219S, 224T, 227P, 228H, 231G, 232A, 234Y, 235A, 238V, 271G, 272D, 278N, 279M, 282T
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

227P

heterodimer interface
215I, 217P, 219S, 224T, 227P, 228H, 231G, 232A, 234Y, 235A, 238V, 271G, 272D, 278N, 279M, 282T
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

228H

heterodimer interface
215I, 217P, 219S, 224T, 227P, 228H, 231G, 232A, 234Y, 235A, 238V, 271G, 272D, 278N, 279M, 282T
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

231G

heterodimer interface
215I, 217P, 219S, 224T, 227P, 228H, 231G, 232A, 234Y, 235A, 238V, 271G, 272D, 278N, 279M, 282T
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

232A

heterodimer interface
215I, 217P, 219S, 224T, 227P, 228H, 231G, 232A, 234Y, 235A, 238V, 271G, 272D, 278N, 279M, 282T
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

234Y

heterodimer interface
215I, 217P, 219S, 224T, 227P, 228H, 231G, 232A, 234Y, 235A, 238V, 271G, 272D, 278N, 279M, 282T
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

235A

heterodimer interface
215I, 217P, 219S, 224T, 227P, 228H, 231G, 232A, 234Y, 235A, 238V, 271G, 272D, 278N, 279M, 282T
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

238V

heterodimer interface
215I, 217P, 219S, 224T, 227P, 228H, 231G, 232A, 234Y, 235A, 238V, 271G, 272D, 278N, 279M, 282T
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

271G

heterodimer interface
215I, 217P, 219S, 224T, 227P, 228H, 231G, 232A, 234Y, 235A, 238V, 271G, 272D, 278N, 279M, 282T
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

272D

heterodimer interface
215I, 217P, 219S, 224T, 227P, 228H, 231G, 232A, 234Y, 235A, 238V, 271G, 272D, 278N, 279M, 282T
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

278N

heterodimer interface
215I, 217P, 219S, 224T, 227P, 228H, 231G, 232A, 234Y, 235A, 238V, 271G, 272D, 278N, 279M, 282T
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

279M

heterodimer interface
215I, 217P, 219S, 224T, 227P, 228H, 231G, 232A, 234Y, 235A, 238V, 271G, 272D, 278N, 279M, 282T
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

282T

heterodimer interface
215I, 217P, 219S, 224T, 227P, 228H, 231G, 232A, 234Y, 235A, 238V, 271G, 272D, 278N, 279M, 282T
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)
phosphorylation lo...

358Y

phosphorylation loop region
358Y, 359R, 360I, 361S, 362H, 363H, 364S, 366S, 367D, 368D, 369S, 370F, 371A, 372Y, 373R, 374S, 375K, 376R, 384K, 385D, 387P
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

359R

phosphorylation loop region
358Y, 359R, 360I, 361S, 362H, 363H, 364S, 366S, 367D, 368D, 369S, 370F, 371A, 372Y, 373R, 374S, 375K, 376R, 384K, 385D, 387P
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

360I

phosphorylation loop region
358Y, 359R, 360I, 361S, 362H, 363H, 364S, 366S, 367D, 368D, 369S, 370F, 371A, 372Y, 373R, 374S, 375K, 376R, 384K, 385D, 387P
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

361S

phosphorylation loop region
358Y, 359R, 360I, 361S, 362H, 363H, 364S, 366S, 367D, 368D, 369S, 370F, 371A, 372Y, 373R, 374S, 375K, 376R, 384K, 385D, 387P
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

362H

phosphorylation loop region
358Y, 359R, 360I, 361S, 362H, 363H, 364S, 366S, 367D, 368D, 369S, 370F, 371A, 372Y, 373R, 374S, 375K, 376R, 384K, 385D, 387P
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

363H

phosphorylation loop region
358Y, 359R, 360I, 361S, 362H, 363H, 364S, 366S, 367D, 368D, 369S, 370F, 371A, 372Y, 373R, 374S, 375K, 376R, 384K, 385D, 387P
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

364S

phosphorylation loop region
358Y, 359R, 360I, 361S, 362H, 363H, 364S, 366S, 367D, 368D, 369S, 370F, 371A, 372Y, 373R, 374S, 375K, 376R, 384K, 385D, 387P
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

366S

phosphorylation loop region
358Y, 359R, 360I, 361S, 362H, 363H, 364S, 366S, 367D, 368D, 369S, 370F, 371A, 372Y, 373R, 374S, 375K, 376R, 384K, 385D, 387P
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

367D

phosphorylation loop region
358Y, 359R, 360I, 361S, 362H, 363H, 364S, 366S, 367D, 368D, 369S, 370F, 371A, 372Y, 373R, 374S, 375K, 376R, 384K, 385D, 387P
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

368D

phosphorylation loop region
358Y, 359R, 360I, 361S, 362H, 363H, 364S, 366S, 367D, 368D, 369S, 370F, 371A, 372Y, 373R, 374S, 375K, 376R, 384K, 385D, 387P
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

369S

phosphorylation loop region
358Y, 359R, 360I, 361S, 362H, 363H, 364S, 366S, 367D, 368D, 369S, 370F, 371A, 372Y, 373R, 374S, 375K, 376R, 384K, 385D, 387P
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

370F

phosphorylation loop region
358Y, 359R, 360I, 361S, 362H, 363H, 364S, 366S, 367D, 368D, 369S, 370F, 371A, 372Y, 373R, 374S, 375K, 376R, 384K, 385D, 387P
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

371A

phosphorylation loop region
358Y, 359R, 360I, 361S, 362H, 363H, 364S, 366S, 367D, 368D, 369S, 370F, 371A, 372Y, 373R, 374S, 375K, 376R, 384K, 385D, 387P
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

372Y

phosphorylation loop region
358Y, 359R, 360I, 361S, 362H, 363H, 364S, 366S, 367D, 368D, 369S, 370F, 371A, 372Y, 373R, 374S, 375K, 376R, 384K, 385D, 387P
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

373R

phosphorylation loop region
358Y, 359R, 360I, 361S, 362H, 363H, 364S, 366S, 367D, 368D, 369S, 370F, 371A, 372Y, 373R, 374S, 375K, 376R, 384K, 385D, 387P
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

374S

phosphorylation loop region
358Y, 359R, 360I, 361S, 362H, 363H, 364S, 366S, 367D, 368D, 369S, 370F, 371A, 372Y, 373R, 374S, 375K, 376R, 384K, 385D, 387P
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

375K

phosphorylation loop region
358Y, 359R, 360I, 361S, 362H, 363H, 364S, 366S, 367D, 368D, 369S, 370F, 371A, 372Y, 373R, 374S, 375K, 376R, 384K, 385D, 387P
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

376R

phosphorylation loop region
358Y, 359R, 360I, 361S, 362H, 363H, 364S, 366S, 367D, 368D, 369S, 370F, 371A, 372Y, 373R, 374S, 375K, 376R, 384K, 385D, 387P
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

384K

phosphorylation loop region
358Y, 359R, 360I, 361S, 362H, 363H, 364S, 366S, 367D, 368D, 369S, 370F, 371A, 372Y, 373R, 374S, 375K, 376R, 384K, 385D, 387P
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

385D

phosphorylation loop region
358Y, 359R, 360I, 361S, 362H, 363H, 364S, 366S, 367D, 368D, 369S, 370F, 371A, 372Y, 373R, 374S, 375K, 376R, 384K, 385D, 387P
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

387P

phosphorylation loop region
358Y, 359R, 360I, 361S, 362H, 363H, 364S, 366S, 367D, 368D, 369S, 370F, 371A, 372Y, 373R, 374S, 375K, 376R, 384K, 385D, 387P
CDD
cd02000 (TPP_E1_PDC_ADC_BCADC)

Protein sequence

>MIA_01651_1
MKTVFNTSFNLPRALLNPSRTKIQRLSIAQRYIHSSLSKKSASILSESHKTPDDSSSPRINKGLDFPGISKTKFVKNLTF
NDPRLLQPMEIYRLIDVDGTQFDKSYEVDVDQDLAIRMYREMHIIGILDNIMYEAQRQGRLSFYMVNAGEEASAIGSASA
LKPNDVIFSQYREAGAILHRGLTVKEFMSQLYANKNDHGHGRSMPVHYMSKTLRIQPISSPLATQIPHAAGAAYALKVLG
EKSGESPKSDNEIDDGACVICYFGDGAASEGDFHAALNMAATLNCPVIFFCRNNGYAISTPTKEQYRGDGIASRGIGYGI
ETIRVDGNDLLAVRRVTQQAREIATRSKKPVLIEAMSYRISHHSTSDDSFAYRSKREVEDWKRKDNPVVRFRKWLEHKDW
WNEDKEAKLKTELKKSILKEFSAGEKTLKPQIRNIFDDMYDTLPETLIEQRRELGEILDTYPDHFDLSHFEGGRQGLGDK

GO term prediction

Biological Process

GO:0008152 metabolic process

Molecular Function

GO:0016624 oxidoreductase activity, acting on the aldehyde or oxo group of donors, disulfide as acceptor

Cellular Component