MIA_01066_1

Protein

Length

339 amino acids

Protein function

EGGNOG:	0PQMD	FG01991.1	hydrolase, carbon-nitrogen family
SGD closest match:	S000004343	NIT3	Probable hydrolase NIT3

Protein alignments

	%id	Aln length	E-value
MCA_02738_1	44.271%	384	2.07e-101	MCA_02738_1
A0A0J9X2Q3_GEOCN	45.294%	340	1.16e-79	Similar to Saccharomyces cerevisiae YLR351C NIT3 Nit protein nitrilase superfamily member OS=Geotrichum candidum GN=BN980_GECA01s04828g PE=4 SV=1
UniRef50_A0A0J9X2Q3	45.294%	340	2.37e-76	Similar to Saccharomyces cerevisiae YLR351C NIT3 Nit protein nitrilase superfamily member n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9X2Q3_GEOCN
NIT3_YEAST	25.000%	196	2.08e-07	Probable hydrolase NIT3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NIT3 PE=1 SV=1
A0A1E3PG54_9ASCO	31.183%	93	6.54e-06	Nit protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_47585 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0463

Protein family membership

None predicted.

Domains and repeats

Domain
1 - 339
Carbon-nitrogen hydrolase (IPR003010)

1 50 100 150 200 250 300 339

Detailed signature matches

IPR003010

Carbon-nitrogen hydrolase
1. G3DSA:3.60.11...
  
  1 - 339
  
  GENE3D
  G3DSA:3.60.110.10 (G3DSA:3.60.110.10)
2. PS50263 (CN_HYDROLASE)
  
  1 - 306
  
  PROSITE profiles
  PS50263 (CN_HYDROLASE)
3. PF00795 (CN_hydrolase)
  
  2 - 189
  
  Pfam
  PF00795 (CN_hydrolase)
4. SSF56317 (Carbon-ni...)
  
  1 - 258
  
  SUPERFAMILY
  SSF56317 (Carbon-nitrogen hydrolase)

no IPR

Unintegrated signatures

cd07197 (nitrilase)

3 - 253

CDD
cd07197 (nitrilase)

Residue annotation

catalytic triad cd...

49E

catalytic triad
49E, 128K, 161C
CDD
cd07197 (nitrilase)

128K

catalytic triad
49E, 128K, 161C
CDD
cd07197 (nitrilase)

161C

catalytic triad
49E, 128K, 161C
CDD
cd07197 (nitrilase)
active site cd07197

49E

active site
49E, 128K, 132W, 135E, 161C, 162W, 164A, 165I, 186F
CDD
cd07197 (nitrilase)

128K

active site
49E, 128K, 132W, 135E, 161C, 162W, 164A, 165I, 186F
CDD
cd07197 (nitrilase)

132W

active site
49E, 128K, 132W, 135E, 161C, 162W, 164A, 165I, 186F
CDD
cd07197 (nitrilase)

135E

active site
49E, 128K, 132W, 135E, 161C, 162W, 164A, 165I, 186F
CDD
cd07197 (nitrilase)

161C

active site
49E, 128K, 132W, 135E, 161C, 162W, 164A, 165I, 186F
CDD
cd07197 (nitrilase)

162W

active site
49E, 128K, 132W, 135E, 161C, 162W, 164A, 165I, 186F
CDD
cd07197 (nitrilase)

164A

active site
49E, 128K, 132W, 135E, 161C, 162W, 164A, 165I, 186F
CDD
cd07197 (nitrilase)

165I

active site
49E, 128K, 132W, 135E, 161C, 162W, 164A, 165I, 186F
CDD
cd07197 (nitrilase)

186F

active site
49E, 128K, 132W, 135E, 161C, 162W, 164A, 165I, 186F
CDD
cd07197 (nitrilase)
dimer interface cd...

129K

dimer interface
129K, 130N, 131L, 132W, 141K, 142G, 143R, 162W, 164A, 165I, 166W, 167P, 168E, 171K, 172Q, 218S, 219A, 221T, 222T, 223R, 225F, 226E, 246G, 247L
CDD
cd07197 (nitrilase)

130N

dimer interface
129K, 130N, 131L, 132W, 141K, 142G, 143R, 162W, 164A, 165I, 166W, 167P, 168E, 171K, 172Q, 218S, 219A, 221T, 222T, 223R, 225F, 226E, 246G, 247L
CDD
cd07197 (nitrilase)

131L

dimer interface
129K, 130N, 131L, 132W, 141K, 142G, 143R, 162W, 164A, 165I, 166W, 167P, 168E, 171K, 172Q, 218S, 219A, 221T, 222T, 223R, 225F, 226E, 246G, 247L
CDD
cd07197 (nitrilase)

132W

dimer interface
129K, 130N, 131L, 132W, 141K, 142G, 143R, 162W, 164A, 165I, 166W, 167P, 168E, 171K, 172Q, 218S, 219A, 221T, 222T, 223R, 225F, 226E, 246G, 247L
CDD
cd07197 (nitrilase)

141K

dimer interface
129K, 130N, 131L, 132W, 141K, 142G, 143R, 162W, 164A, 165I, 166W, 167P, 168E, 171K, 172Q, 218S, 219A, 221T, 222T, 223R, 225F, 226E, 246G, 247L
CDD
cd07197 (nitrilase)

142G

dimer interface
129K, 130N, 131L, 132W, 141K, 142G, 143R, 162W, 164A, 165I, 166W, 167P, 168E, 171K, 172Q, 218S, 219A, 221T, 222T, 223R, 225F, 226E, 246G, 247L
CDD
cd07197 (nitrilase)

143R

dimer interface
129K, 130N, 131L, 132W, 141K, 142G, 143R, 162W, 164A, 165I, 166W, 167P, 168E, 171K, 172Q, 218S, 219A, 221T, 222T, 223R, 225F, 226E, 246G, 247L
CDD
cd07197 (nitrilase)

162W

dimer interface
129K, 130N, 131L, 132W, 141K, 142G, 143R, 162W, 164A, 165I, 166W, 167P, 168E, 171K, 172Q, 218S, 219A, 221T, 222T, 223R, 225F, 226E, 246G, 247L
CDD
cd07197 (nitrilase)

164A

dimer interface
129K, 130N, 131L, 132W, 141K, 142G, 143R, 162W, 164A, 165I, 166W, 167P, 168E, 171K, 172Q, 218S, 219A, 221T, 222T, 223R, 225F, 226E, 246G, 247L
CDD
cd07197 (nitrilase)

165I

dimer interface
129K, 130N, 131L, 132W, 141K, 142G, 143R, 162W, 164A, 165I, 166W, 167P, 168E, 171K, 172Q, 218S, 219A, 221T, 222T, 223R, 225F, 226E, 246G, 247L
CDD
cd07197 (nitrilase)

166W

dimer interface
129K, 130N, 131L, 132W, 141K, 142G, 143R, 162W, 164A, 165I, 166W, 167P, 168E, 171K, 172Q, 218S, 219A, 221T, 222T, 223R, 225F, 226E, 246G, 247L
CDD
cd07197 (nitrilase)

167P

dimer interface
129K, 130N, 131L, 132W, 141K, 142G, 143R, 162W, 164A, 165I, 166W, 167P, 168E, 171K, 172Q, 218S, 219A, 221T, 222T, 223R, 225F, 226E, 246G, 247L
CDD
cd07197 (nitrilase)

168E

dimer interface
129K, 130N, 131L, 132W, 141K, 142G, 143R, 162W, 164A, 165I, 166W, 167P, 168E, 171K, 172Q, 218S, 219A, 221T, 222T, 223R, 225F, 226E, 246G, 247L
CDD
cd07197 (nitrilase)

171K

dimer interface
129K, 130N, 131L, 132W, 141K, 142G, 143R, 162W, 164A, 165I, 166W, 167P, 168E, 171K, 172Q, 218S, 219A, 221T, 222T, 223R, 225F, 226E, 246G, 247L
CDD
cd07197 (nitrilase)

172Q

dimer interface
129K, 130N, 131L, 132W, 141K, 142G, 143R, 162W, 164A, 165I, 166W, 167P, 168E, 171K, 172Q, 218S, 219A, 221T, 222T, 223R, 225F, 226E, 246G, 247L
CDD
cd07197 (nitrilase)

218S

dimer interface
129K, 130N, 131L, 132W, 141K, 142G, 143R, 162W, 164A, 165I, 166W, 167P, 168E, 171K, 172Q, 218S, 219A, 221T, 222T, 223R, 225F, 226E, 246G, 247L
CDD
cd07197 (nitrilase)

219A

dimer interface
129K, 130N, 131L, 132W, 141K, 142G, 143R, 162W, 164A, 165I, 166W, 167P, 168E, 171K, 172Q, 218S, 219A, 221T, 222T, 223R, 225F, 226E, 246G, 247L
CDD
cd07197 (nitrilase)

221T

dimer interface
129K, 130N, 131L, 132W, 141K, 142G, 143R, 162W, 164A, 165I, 166W, 167P, 168E, 171K, 172Q, 218S, 219A, 221T, 222T, 223R, 225F, 226E, 246G, 247L
CDD
cd07197 (nitrilase)

222T

dimer interface
129K, 130N, 131L, 132W, 141K, 142G, 143R, 162W, 164A, 165I, 166W, 167P, 168E, 171K, 172Q, 218S, 219A, 221T, 222T, 223R, 225F, 226E, 246G, 247L
CDD
cd07197 (nitrilase)

223R

dimer interface
129K, 130N, 131L, 132W, 141K, 142G, 143R, 162W, 164A, 165I, 166W, 167P, 168E, 171K, 172Q, 218S, 219A, 221T, 222T, 223R, 225F, 226E, 246G, 247L
CDD
cd07197 (nitrilase)

225F

dimer interface
129K, 130N, 131L, 132W, 141K, 142G, 143R, 162W, 164A, 165I, 166W, 167P, 168E, 171K, 172Q, 218S, 219A, 221T, 222T, 223R, 225F, 226E, 246G, 247L
CDD
cd07197 (nitrilase)

226E

dimer interface
129K, 130N, 131L, 132W, 141K, 142G, 143R, 162W, 164A, 165I, 166W, 167P, 168E, 171K, 172Q, 218S, 219A, 221T, 222T, 223R, 225F, 226E, 246G, 247L
CDD
cd07197 (nitrilase)

246G

dimer interface
129K, 130N, 131L, 132W, 141K, 142G, 143R, 162W, 164A, 165I, 166W, 167P, 168E, 171K, 172Q, 218S, 219A, 221T, 222T, 223R, 225F, 226E, 246G, 247L
CDD
cd07197 (nitrilase)

247L

dimer interface
129K, 130N, 131L, 132W, 141K, 142G, 143R, 162W, 164A, 165I, 166W, 167P, 168E, 171K, 172Q, 218S, 219A, 221T, 222T, 223R, 225F, 226E, 246G, 247L
CDD
cd07197 (nitrilase)

Protein sequence

>MIA_01066_1
MKLALIQQDIEDASPQKNVEKALHLIRTAVSQELAKSPAAPVDLVVLPEFLQGNPNQPHPPHFVDTEQVVLARFCDLARE
LAVCIVPGTISHYEPDVQQDQDPTNKTILLNTAYFIDRAGNVAGEYHKKNLWHSERPKYTKGREPHTVFDTEFGKTGMLI
CWDAIWPEAFKQLAAQKVDLIVIPSFWTGADNLVPEDEDTAGINATVPADSESVFLRSAMTTRAFESNALVVYTNVGGLP
SRTFIGLSQVALPMWGVLPATRVYDVVGLDALTTEQVAALEPGAHVTRVEESQPGVLETSQDAKDRIAIVEVNKVAEKLA
HAEQVYKIRADAESEDWHY

GO term prediction

Biological Process

GO:0006807 nitrogen compound metabolic process

Molecular Function

GO:0016810 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

Cellular Component