MCA_02738_1

Protein

Length

374 amino acids

Browser: contigB:2221930-2223125+

RNA-seq: read pairs 858, FPKM 28.3, percentile rank 51.0% (100% = highest expression)

Protein function

EGGNOG:	0PQMD	FG01991.1	hydrolase, carbon-nitrogen family

Protein alignments

	%id	Aln length	E-value
MIA_01066_1	44.27%	384	8e-100	MIA_01066_1
A0A0J9X2Q3_GEOCN	37.10%	372	3e-67	Similar to Saccharomyces cerevisiae YLR351C NIT3 Nit protein nitrilase superfamily member OS=Geotrichum candidum GN=BN980_GECA01s04828g PE=4 SV=1
UniRef50_A0A0J9X2Q3	37.10%	372	7e-64	Similar to Saccharomyces cerevisiae YLR351C NIT3 Nit protein nitrilase superfamily member n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9X2Q3_GEOCN

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0137

Protein family membership

None predicted.

Domains and repeats

Domain
1 - 339
Carbon-nitrogen hydrolase (IPR003010)

1 50 100 150 200 250 300 374

Detailed signature matches

IPR003010

Carbon-nitrogen hydrolase
1. G3DSA:3.60.11...
  
  1 - 319
  
  GENE3D
  G3DSA:3.60.110.10 (G3DSA:3.60.110.10)
2. PS50263 (CN_HYDROLASE)
  
  1 - 339
  
  PROSITE profiles
  PS50263 (CN_HYDROLASE)
3. PF00795 (CN_hydrolase)
  
  2 - 231
  
  Pfam
  PF00795 (CN_hydrolase)
4. SSF56317 (Carbon-ni...)
  
  1 - 143
  
  SUPERFAMILY
  SSF56317 (Carbon-nitrogen hydrolase)
  
  180 - 301
  
  SUPERFAMILY
  SSF56317 (Carbon-nitrogen hydrolase)

no IPR

Unintegrated signatures

cd07197 (nitrilase)

3 - 301

CDD
cd07197 (nitrilase)

Residue annotation

catalytic triad cd...

50E

catalytic triad
50E, 133K, 202C
CDD
cd07197 (nitrilase)

133K

catalytic triad
50E, 133K, 202C
CDD
cd07197 (nitrilase)

202C

catalytic triad
50E, 133K, 202C
CDD
cd07197 (nitrilase)
active site cd07197

50E

active site
50E, 133K, 137W, 141R, 202C, 203W, 205V, 206I, 227F
CDD
cd07197 (nitrilase)

133K

active site
50E, 133K, 137W, 141R, 202C, 203W, 205V, 206I, 227F
CDD
cd07197 (nitrilase)

137W

active site
50E, 133K, 137W, 141R, 202C, 203W, 205V, 206I, 227F
CDD
cd07197 (nitrilase)

141R

active site
50E, 133K, 137W, 141R, 202C, 203W, 205V, 206I, 227F
CDD
cd07197 (nitrilase)

202C

active site
50E, 133K, 137W, 141R, 202C, 203W, 205V, 206I, 227F
CDD
cd07197 (nitrilase)

203W

active site
50E, 133K, 137W, 141R, 202C, 203W, 205V, 206I, 227F
CDD
cd07197 (nitrilase)

205V

active site
50E, 133K, 137W, 141R, 202C, 203W, 205V, 206I, 227F
CDD
cd07197 (nitrilase)

206I

active site
50E, 133K, 137W, 141R, 202C, 203W, 205V, 206I, 227F
CDD
cd07197 (nitrilase)

227F

active site
50E, 133K, 137W, 141R, 202C, 203W, 205V, 206I, 227F
CDD
cd07197 (nitrilase)
dimer interface cd...

134K

dimer interface
134K, 135N, 136I, 137W, 140E, 147A, 148T, 149D, 203W, 205V, 206I, 207F, 208S, 209E, 212K, 213Q, 261H, 262A, 264V, 265T, 266R, 268F, 269E, 289G, 290L
CDD
cd07197 (nitrilase)

135N

dimer interface
134K, 135N, 136I, 137W, 140E, 147A, 148T, 149D, 203W, 205V, 206I, 207F, 208S, 209E, 212K, 213Q, 261H, 262A, 264V, 265T, 266R, 268F, 269E, 289G, 290L
CDD
cd07197 (nitrilase)

136I

dimer interface
134K, 135N, 136I, 137W, 140E, 147A, 148T, 149D, 203W, 205V, 206I, 207F, 208S, 209E, 212K, 213Q, 261H, 262A, 264V, 265T, 266R, 268F, 269E, 289G, 290L
CDD
cd07197 (nitrilase)

137W

dimer interface
134K, 135N, 136I, 137W, 140E, 147A, 148T, 149D, 203W, 205V, 206I, 207F, 208S, 209E, 212K, 213Q, 261H, 262A, 264V, 265T, 266R, 268F, 269E, 289G, 290L
CDD
cd07197 (nitrilase)

140E

dimer interface
134K, 135N, 136I, 137W, 140E, 147A, 148T, 149D, 203W, 205V, 206I, 207F, 208S, 209E, 212K, 213Q, 261H, 262A, 264V, 265T, 266R, 268F, 269E, 289G, 290L
CDD
cd07197 (nitrilase)

147A

dimer interface
134K, 135N, 136I, 137W, 140E, 147A, 148T, 149D, 203W, 205V, 206I, 207F, 208S, 209E, 212K, 213Q, 261H, 262A, 264V, 265T, 266R, 268F, 269E, 289G, 290L
CDD
cd07197 (nitrilase)

148T

dimer interface
134K, 135N, 136I, 137W, 140E, 147A, 148T, 149D, 203W, 205V, 206I, 207F, 208S, 209E, 212K, 213Q, 261H, 262A, 264V, 265T, 266R, 268F, 269E, 289G, 290L
CDD
cd07197 (nitrilase)

149D

dimer interface
134K, 135N, 136I, 137W, 140E, 147A, 148T, 149D, 203W, 205V, 206I, 207F, 208S, 209E, 212K, 213Q, 261H, 262A, 264V, 265T, 266R, 268F, 269E, 289G, 290L
CDD
cd07197 (nitrilase)

203W

dimer interface
134K, 135N, 136I, 137W, 140E, 147A, 148T, 149D, 203W, 205V, 206I, 207F, 208S, 209E, 212K, 213Q, 261H, 262A, 264V, 265T, 266R, 268F, 269E, 289G, 290L
CDD
cd07197 (nitrilase)

205V

dimer interface
134K, 135N, 136I, 137W, 140E, 147A, 148T, 149D, 203W, 205V, 206I, 207F, 208S, 209E, 212K, 213Q, 261H, 262A, 264V, 265T, 266R, 268F, 269E, 289G, 290L
CDD
cd07197 (nitrilase)

206I

dimer interface
134K, 135N, 136I, 137W, 140E, 147A, 148T, 149D, 203W, 205V, 206I, 207F, 208S, 209E, 212K, 213Q, 261H, 262A, 264V, 265T, 266R, 268F, 269E, 289G, 290L
CDD
cd07197 (nitrilase)

207F

dimer interface
134K, 135N, 136I, 137W, 140E, 147A, 148T, 149D, 203W, 205V, 206I, 207F, 208S, 209E, 212K, 213Q, 261H, 262A, 264V, 265T, 266R, 268F, 269E, 289G, 290L
CDD
cd07197 (nitrilase)

208S

dimer interface
134K, 135N, 136I, 137W, 140E, 147A, 148T, 149D, 203W, 205V, 206I, 207F, 208S, 209E, 212K, 213Q, 261H, 262A, 264V, 265T, 266R, 268F, 269E, 289G, 290L
CDD
cd07197 (nitrilase)

209E

dimer interface
134K, 135N, 136I, 137W, 140E, 147A, 148T, 149D, 203W, 205V, 206I, 207F, 208S, 209E, 212K, 213Q, 261H, 262A, 264V, 265T, 266R, 268F, 269E, 289G, 290L
CDD
cd07197 (nitrilase)

212K

dimer interface
134K, 135N, 136I, 137W, 140E, 147A, 148T, 149D, 203W, 205V, 206I, 207F, 208S, 209E, 212K, 213Q, 261H, 262A, 264V, 265T, 266R, 268F, 269E, 289G, 290L
CDD
cd07197 (nitrilase)

213Q

dimer interface
134K, 135N, 136I, 137W, 140E, 147A, 148T, 149D, 203W, 205V, 206I, 207F, 208S, 209E, 212K, 213Q, 261H, 262A, 264V, 265T, 266R, 268F, 269E, 289G, 290L
CDD
cd07197 (nitrilase)

261H

dimer interface
134K, 135N, 136I, 137W, 140E, 147A, 148T, 149D, 203W, 205V, 206I, 207F, 208S, 209E, 212K, 213Q, 261H, 262A, 264V, 265T, 266R, 268F, 269E, 289G, 290L
CDD
cd07197 (nitrilase)

262A

dimer interface
134K, 135N, 136I, 137W, 140E, 147A, 148T, 149D, 203W, 205V, 206I, 207F, 208S, 209E, 212K, 213Q, 261H, 262A, 264V, 265T, 266R, 268F, 269E, 289G, 290L
CDD
cd07197 (nitrilase)

264V

dimer interface
134K, 135N, 136I, 137W, 140E, 147A, 148T, 149D, 203W, 205V, 206I, 207F, 208S, 209E, 212K, 213Q, 261H, 262A, 264V, 265T, 266R, 268F, 269E, 289G, 290L
CDD
cd07197 (nitrilase)

265T

dimer interface
134K, 135N, 136I, 137W, 140E, 147A, 148T, 149D, 203W, 205V, 206I, 207F, 208S, 209E, 212K, 213Q, 261H, 262A, 264V, 265T, 266R, 268F, 269E, 289G, 290L
CDD
cd07197 (nitrilase)

266R

dimer interface
134K, 135N, 136I, 137W, 140E, 147A, 148T, 149D, 203W, 205V, 206I, 207F, 208S, 209E, 212K, 213Q, 261H, 262A, 264V, 265T, 266R, 268F, 269E, 289G, 290L
CDD
cd07197 (nitrilase)

268F

dimer interface
134K, 135N, 136I, 137W, 140E, 147A, 148T, 149D, 203W, 205V, 206I, 207F, 208S, 209E, 212K, 213Q, 261H, 262A, 264V, 265T, 266R, 268F, 269E, 289G, 290L
CDD
cd07197 (nitrilase)

269E

dimer interface
134K, 135N, 136I, 137W, 140E, 147A, 148T, 149D, 203W, 205V, 206I, 207F, 208S, 209E, 212K, 213Q, 261H, 262A, 264V, 265T, 266R, 268F, 269E, 289G, 290L
CDD
cd07197 (nitrilase)

289G

dimer interface
134K, 135N, 136I, 137W, 140E, 147A, 148T, 149D, 203W, 205V, 206I, 207F, 208S, 209E, 212K, 213Q, 261H, 262A, 264V, 265T, 266R, 268F, 269E, 289G, 290L
CDD
cd07197 (nitrilase)

290L

dimer interface
134K, 135N, 136I, 137W, 140E, 147A, 148T, 149D, 203W, 205V, 206I, 207F, 208S, 209E, 212K, 213Q, 261H, 262A, 264V, 265T, 266R, 268F, 269E, 289G, 290L
CDD
cd07197 (nitrilase)

Protein sequence

>MCA_02738_1
MKIALIQQELEFENAKKNIQLALKLTREAVKQCYNDETGSHDLDLVILPEFLLGSPHQEDPAHFVDFHDPFTSKESVVFQ
FQQLAKELKVCIVPGSIPRPMKSDESKIENKEQYLNTSYFIDREGKILGLYNKKNIWISERPKFSVATDEHIEQLKATGG
HADSQGVSAPLEDLSKYDGNKDGKQYHRVFETEFGKTAILICWDVIFSEAFKQLAQQDVDLIIVPSFWTGDDNLLPGQEK
DSKDSNVLTAPADSEVVLLKHALVTRAFESEALVAYANVGGKPSEGYIGLSQVAMPLWGNIPGTVYDVKNTEQDSLELEK
LPEPSTVLDVKEPSPNLIQIVQVDQWKEKLELSEKIYQIRSDMKRSNWHYPTCY

GO term prediction

Biological Process

GO:0006807 nitrogen compound metabolic process

Molecular Function

GO:0016810 hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds

Cellular Component