MIA_01007_1

Protein

Length

874 amino acids

Protein function

EGGNOG:	0PFTQ	GPH1	Phosphorylase is an important allosteric enzyme in carbohydrate metabolism. Enzymes from different sources differ in their regulatory mechanisms and in their natural substrates. However, all known phosphorylases share catalytic and structural properties (By similarity)
SGD closest match:	S000006364	GPH1	Glycogen phosphorylase
CGD closest match:	CAL0000189831	GPH1	Alpha-1,4 glucan phosphorylase

Protein alignments

	%id	Aln length
A0A0J9X5Z1_GEOCN	74.46%	873	Alpha-1,4 glucan phosphorylase OS=Geotrichum candidum GN=BN980_GECA03s04388g PE=3 SV=1
MCA_02090_1	77.97%	808	MCA_02090_1
Q6C2Y0_YARLI	69.85%	859	Alpha-1,4 glucan phosphorylase OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_F04169g PE=3 SV=1
A0A1E3PQD2_9ASCO	68.76%	861	Alpha-1,4 glucan phosphorylase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_40802 PE=3 SV=1
A0A167DDG6_9ASCO	69.26%	849	Alpha-1,4 glucan phosphorylase OS=Sugiyamaella lignohabitans GN=GPH1 PE=3 SV=1
A0A060T314_BLAAD	67.25%	858	Alpha-1,4 glucan phosphorylase OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C29238g PE=3 SV=1
UniRef50_Q0U454	64.78%	866	Alpha-1,4 glucan phosphorylase n=1 Tax=Phaeosphaeria nodorum (strain SN15 / ATCC MYA-4574 / FGSC 10173) TaxID=321614 RepID=Q0U454_PHANO
PHSG_YEAST	61.43%	892	Glycogen phosphorylase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GPH1 PE=1 SV=4
A0A1D8PQQ3_CANAL	60.16%	891	Alpha-1,4 glucan phosphorylase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=GPH1 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0922

Protein family membership

Glycosyl transferase, family 35 (IPR000811)
- Glycogen/starch/alpha-glucan phosphorylase (IPR011833)

Domains and repeats

None predicted.

Detailed signature matches

IPR000811

Glycosyl transferase, family 35
1. PIRSF000460 (Glucan...)
  
  49 - 874
  
  PIRSF
  PIRSF000460 (Glucan_phosphorylase_GlgP)
2. PF00343 (Phosphorylase)
  
  153 - 867
  
  Pfam
  PF00343 (Phosphorylase)
IPR011833

Glycogen/starch/alpha-glucan phosphorylase
1. TIGR02093
  
  64 - 867
  
  TIGRFAMs
  TIGR02093 (TIGR02093)
2. cd04300 (GT1_Glycog...)
  
  68 - 867
  
  CDD
  cd04300 (GT1_Glycogen_Phosphorylase)
IPR035090

Phosphorylase pyridoxal-phosphate attachment site
1. PS00102 (PHOSPHORYLASE)
  
  714 - 726
  
  PROSITE patterns
  PS00102 (PHOSPHORYLASE)

no IPR

Unintegrated signatures

G3DSA:3.40.50...

535 - 852

GENE3D
G3DSA:3.40.50.2000 (G3DSA:3.40.50.2000)

54 - 534

GENE3D
G3DSA:3.40.50.2000 (G3DSA:3.40.50.2000)
SSF53756 (UDP-Glyco...)

30 - 870

SUPERFAMILY
SSF53756 (UDP-Glycosyltransferase/glycogen phosphorylase)

Residue annotation

homodimer interfac...

68Q

homodimer interface
68Q, 70E, 72V, 73R, 75V, 76E, 77T, 100R, 101D, 102R, 233R, 327N, 330F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

70E

homodimer interface
68Q, 70E, 72V, 73R, 75V, 76E, 77T, 100R, 101D, 102R, 233R, 327N, 330F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

72V

homodimer interface
68Q, 70E, 72V, 73R, 75V, 76E, 77T, 100R, 101D, 102R, 233R, 327N, 330F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

73R

homodimer interface
68Q, 70E, 72V, 73R, 75V, 76E, 77T, 100R, 101D, 102R, 233R, 327N, 330F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

75V

homodimer interface
68Q, 70E, 72V, 73R, 75V, 76E, 77T, 100R, 101D, 102R, 233R, 327N, 330F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

76E

homodimer interface
68Q, 70E, 72V, 73R, 75V, 76E, 77T, 100R, 101D, 102R, 233R, 327N, 330F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

77T

homodimer interface
68Q, 70E, 72V, 73R, 75V, 76E, 77T, 100R, 101D, 102R, 233R, 327N, 330F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

100R

homodimer interface
68Q, 70E, 72V, 73R, 75V, 76E, 77T, 100R, 101D, 102R, 233R, 327N, 330F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

101D

homodimer interface
68Q, 70E, 72V, 73R, 75V, 76E, 77T, 100R, 101D, 102R, 233R, 327N, 330F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

102R

homodimer interface
68Q, 70E, 72V, 73R, 75V, 76E, 77T, 100R, 101D, 102R, 233R, 327N, 330F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

233R

homodimer interface
68Q, 70E, 72V, 73R, 75V, 76E, 77T, 100R, 101D, 102R, 233R, 327N, 330F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

327N

homodimer interface
68Q, 70E, 72V, 73R, 75V, 76E, 77T, 100R, 101D, 102R, 233R, 327N, 330F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

330F

homodimer interface
68Q, 70E, 72V, 73R, 75V, 76E, 77T, 100R, 101D, 102R, 233R, 327N, 330F
CDD
cd04300 (GT1_Glycogen_Phosphorylase)
active site pocket...

128E

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

130L

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

174G

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

175G

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

176L

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

325Y

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

328D

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

330F

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

337R

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

376D

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

378H

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

414H

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

415T

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

419E

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

522N

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

529W

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

607K

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

608R

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

610H

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

611E

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

612Y

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

613K

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

650K

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

655Y

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

657L

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

691N

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

692V

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

714E

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

716S

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

717G

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

718T

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

719S

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

722K

active site pocket
128E, 130L, 174G, 175G, 176L, 325Y, 328D, 330F, 337R, 376D, 378H, 414H, 415T, 419E, 522N, 529W, 607K, 608R, 610H, 611E, 612Y, 613K, 650K, 655Y, 657L, 691N, 692V, 714E, 716S, 717G, 718T, 719S, 722K
CDD
cd04300 (GT1_Glycogen_Phosphorylase)

Protein sequence

>MIA_01007_1
MSFFNKKYADPLYDDDEPSYPLPRRFKRTYTGFTPKELKTIDSTVPAEFRNTWKKFAVGEFKSKQQFQSEFVRHVETTLA
RSLYNCDELAAYQAASQAIRDRLVIAWNKTQQKHTTVDPKRIYYLSLEFLMGRALDNALLNLEVKEAAGDGIKDFGFRLE
DLIEQEKDAALGNGGLGRLAACFIDSLTTLNYPGWGYGLRYNYGIFKQKIVDGYQVEMPDYWLTFNNPWEIPRTEIAIDI
MFYGYVRKTRTDDGKEKKIWEGGQVVQAVAYDLPVPGYATENVNNLRLWSSEPSREFDFAKFNEGSYNEALREQQEAETI
SAVLYPNDNFPSGKELRLKQQYFWVAASLSDIVRRFKKTQRPWVEFPSQVAIQLNDTHPTLAVPELQRILVDLEGLEWDE
AWNIVTSTFSYTNHTVLSEALEKWSVGLFGNLLPRHLDIIYDINYRFLQFVGAKFPNEADLRRDVSIFEEKDDQKVRMAY
LAIIGSHKVNGVARLHSDLIKETIFSQFVRVYGPEKFINVTNGVTPRRWLHQANPALSAFIAEKLGGYEYLNKLDQLKGL
LKYKDDPEFQKKWMDIKLANKARLAALVKEQSGVELDLNALFDIQVKRIHEYKRQQMNIFSVIWRYLKLKTLSPAELAKV
QPRVSIFGGKAAPGYYLAKTVIKLINSVSQVVNNDATIDGKLKVVFIEDYNVSKAEIIIPASDISEHISTAGTEASGTSN
MKFVLNGGLIIGTVDGANIEITEQVGEDNIFLFGHLAKDVEGLRYTHRSGGSVLDPQLAQVFAAIDSGIFGDPTIYGDLS
TAIVGYGGDQYLVSDDFASYLEAHDKIDETFADKKVWATKSITAVANMGFFSSDRAIEDYARDIWDIKPVPLNE

GO term prediction

Biological Process

GO:0005975 carbohydrate metabolic process

Molecular Function

GO:0004645 phosphorylase activity
GO:0008184 glycogen phosphorylase activity
GO:0030170 pyridoxal phosphate binding

Cellular Component