MIA_00954_1

Protein

Length

3,624 amino acids

Protein function

EGGNOG:	0PHS9	PGUG_02920	Ubiquitin-protein ligase
SGD closest match:	S000002865	TOM1	E3 ubiquitin-protein ligase TOM1
CGD closest match:	CAL0000176024	TOM1	E3 ubiquitin-protein ligase

Protein alignments

	%id	Aln length
MCA_00580_1	50.568%	1847	MCA_00580_1
A0A0J9XGS3_GEOCN	35.130%	2115	Similar to Saccharomyces cerevisiae YDR457W TOM1 E3 ubiquitin ligase of the hect-domain class OS=Geotrichum candidum GN=BN980_GECA16s00373g PE=4 SV=1
UniRef50_A0A0J9XGS3	35.130%	2115	Similar to Saccharomyces cerevisiae YDR457W TOM1 E3 ubiquitin ligase of the hect-domain class n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9XGS3_GEOCN
A0A170QY39_9ASCO	52.344%	1173	E3 ubiquitin-protein ligase TOM1 OS=Sugiyamaella lignohabitans GN=TOM1 PE=4 SV=1
A0A060SYY9_BLAAD	50.511%	1174	ARAD1C01144p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C01144g PE=4 SV=1
A0A1E3PGK5_9ASCO	49.306%	1152	Uncharacterized protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_52972 PE=4 SV=1
Q6CFL1_YARLI	47.354%	1134	YALI0B05940p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_B05940g PE=4 SV=2
TOM1_YEAST	42.764%	1071	E3 ubiquitin-protein ligase TOM1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TOM1 PE=1 SV=1
A0A1D8PGT3_CANAL	58.162%	631	E3 ubiquitin-protein ligase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=TOM1 PE=4 SV=1
A0A1E4TJX7_9ASCO	55.643%	638	Uncharacterized protein (Fragment) OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_14432 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.1714
Predicted cleavage: 21

Protein family membership

None predicted.

Domains and repeats

Domain
90 - 416
E3 ubiquitin ligase, domain of unknown function DUF908 (IPR010309)
485 - 713
E3 ubiquitin ligase, domain of unknown function DUF913 (IPR010314)
2,532 - 2,629
Domain of unknown function DUF4414 (IPR025527)
Domain
118 - 2,069
Armadillo-type fold (IPR016024)
Armadillo-like helical (IPR011989)
2,417 - 3,224
Armadillo-like helical (IPR011989)
3,252 - 3,624
HECT domain (IPR000569)

1 500 1000 1500 2000 2500 3000 3624

Detailed signature matches

IPR010309

E3 ubiquitin ligase, domain of unknown function DUF908
1. PF06012 (DUF908)
  
  90 - 416
  
  Pfam
  PF06012 (DUF908)
IPR016024

Armadillo-type fold
1. SSF48371 (ARM repeat)
  
  593 - 790
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
  
  447 - 520
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
  
  323 - 400
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
IPR011989

Armadillo-like helical
1. G3DSA:1.25.10.10
  
  2,417 - 3,224
  
  GENE3D
  G3DSA:1.25.10.10 (G3DSA:1.25.10.10)
  
  118 - 2,069
  
  GENE3D
  G3DSA:1.25.10.10 (G3DSA:1.25.10.10)
IPR010314

E3 ubiquitin ligase, domain of unknown function DUF913
1. PF06025 (DUF913)
  
  485 - 713
  
  Pfam
  PF06025 (DUF913)
IPR025527

Domain of unknown function DUF4414
1. PF14377 (DUF4414)
  
  2,532 - 2,629
  
  Pfam
  PF14377 (DUF4414)
IPR000569

HECT domain
1. PF00632 (HECT)
  
  3,319 - 3,623
  
  Pfam
  PF00632 (HECT)
2. cd00078 (HECTc)
  
  3,267 - 3,622
  
  CDD
  cd00078 (HECTc)
3. SSF56204 (Hect, E3 ...)
  
  3,252 - 3,617
  
  SUPERFAMILY
  SSF56204 (Hect, E3 ligase catalytic domain)
4. SM00119 (hect_3)
  
  3,286 - 3,624
  
  SMART
  SM00119 (hect_3)
5. PS50237 (HECT)
  
  3,288 - 3,624
  
  PROSITE profiles
  PS50237 (HECT)

no IPR

Unintegrated signatures

CYTOPLASMIC_D... (C...)

3,169 - 3,624

PHOBIUS
CYTOPLASMIC_DOMAIN (Cytoplasmic domain)
Coil

1,653 - 1,673

COILS
Coil (Coil)

2,408 - 2,479

COILS
Coil (Coil)
G3DSA:3.30.24...

3,505 - 3,624

GENE3D
G3DSA:3.30.2410.10 (G3DSA:3.30.2410.10)
G3DSA:3.90.17...

3,242 - 3,423

GENE3D
G3DSA:3.90.1750.10 (G3DSA:3.90.1750.10)
NON_CYTOPLASM... (N...)

1 - 3,149

PHOBIUS
NON_CYTOPLASMIC_DOMAIN (Non cytoplasmic domain)
TRANSMEMBRANE (Tran...)

3,150 - 3,168

PHOBIUS
TRANSMEMBRANE (Transmembrane region)
mobidb-lite (disord...)

2,182 - 2,238

MobiDB Lite
mobidb-lite (disorder_prediction)

2,923 - 2,992

MobiDB Lite
mobidb-lite (disorder_prediction)

2,429 - 2,513

MobiDB Lite
mobidb-lite (disorder_prediction)

206 - 262

MobiDB Lite
mobidb-lite (disorder_prediction)

2,087 - 2,131

MobiDB Lite
mobidb-lite (disorder_prediction)

1,682 - 1,729

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

catalytic cleft cd...

3,273R

catalytic cleft
3,273R, 3,304E, 3,315E, 3,376D, 3,549F, 3,585L, 3,586P, 3,589H, 3,590T, 3,591C, 3,592F, 3,593N, 3,613A, 3,621F
CDD
cd00078 (HECTc)

3,304E

catalytic cleft
3,273R, 3,304E, 3,315E, 3,376D, 3,549F, 3,585L, 3,586P, 3,589H, 3,590T, 3,591C, 3,592F, 3,593N, 3,613A, 3,621F
CDD
cd00078 (HECTc)

3,315E

catalytic cleft
3,273R, 3,304E, 3,315E, 3,376D, 3,549F, 3,585L, 3,586P, 3,589H, 3,590T, 3,591C, 3,592F, 3,593N, 3,613A, 3,621F
CDD
cd00078 (HECTc)

3,376D

catalytic cleft
3,273R, 3,304E, 3,315E, 3,376D, 3,549F, 3,585L, 3,586P, 3,589H, 3,590T, 3,591C, 3,592F, 3,593N, 3,613A, 3,621F
CDD
cd00078 (HECTc)

3,549F

catalytic cleft
3,273R, 3,304E, 3,315E, 3,376D, 3,549F, 3,585L, 3,586P, 3,589H, 3,590T, 3,591C, 3,592F, 3,593N, 3,613A, 3,621F
CDD
cd00078 (HECTc)

3,585L

catalytic cleft
3,273R, 3,304E, 3,315E, 3,376D, 3,549F, 3,585L, 3,586P, 3,589H, 3,590T, 3,591C, 3,592F, 3,593N, 3,613A, 3,621F
CDD
cd00078 (HECTc)

3,586P

catalytic cleft
3,273R, 3,304E, 3,315E, 3,376D, 3,549F, 3,585L, 3,586P, 3,589H, 3,590T, 3,591C, 3,592F, 3,593N, 3,613A, 3,621F
CDD
cd00078 (HECTc)

3,589H

catalytic cleft
3,273R, 3,304E, 3,315E, 3,376D, 3,549F, 3,585L, 3,586P, 3,589H, 3,590T, 3,591C, 3,592F, 3,593N, 3,613A, 3,621F
CDD
cd00078 (HECTc)

3,590T

catalytic cleft
3,273R, 3,304E, 3,315E, 3,376D, 3,549F, 3,585L, 3,586P, 3,589H, 3,590T, 3,591C, 3,592F, 3,593N, 3,613A, 3,621F
CDD
cd00078 (HECTc)

3,591C

catalytic cleft
3,273R, 3,304E, 3,315E, 3,376D, 3,549F, 3,585L, 3,586P, 3,589H, 3,590T, 3,591C, 3,592F, 3,593N, 3,613A, 3,621F
CDD
cd00078 (HECTc)

3,592F

catalytic cleft
3,273R, 3,304E, 3,315E, 3,376D, 3,549F, 3,585L, 3,586P, 3,589H, 3,590T, 3,591C, 3,592F, 3,593N, 3,613A, 3,621F
CDD
cd00078 (HECTc)

3,593N

catalytic cleft
3,273R, 3,304E, 3,315E, 3,376D, 3,549F, 3,585L, 3,586P, 3,589H, 3,590T, 3,591C, 3,592F, 3,593N, 3,613A, 3,621F
CDD
cd00078 (HECTc)

3,613A

catalytic cleft
3,273R, 3,304E, 3,315E, 3,376D, 3,549F, 3,585L, 3,586P, 3,589H, 3,590T, 3,591C, 3,592F, 3,593N, 3,613A, 3,621F
CDD
cd00078 (HECTc)

3,621F

catalytic cleft
3,273R, 3,304E, 3,315E, 3,376D, 3,549F, 3,585L, 3,586P, 3,589H, 3,590T, 3,591C, 3,592F, 3,593N, 3,613A, 3,621F
CDD
cd00078 (HECTc)
E2 interaction sit...

3,404Y

E2 interaction site
3,404Y, 3,407S, 3,408L, 3,410W, 3,411M, 3,414N, 3,420I, 3,422E, 3,426I, 3,427E, 3,429D, 3,434T, 3,439L, 3,456Y, 3,460V
CDD
cd00078 (HECTc)

3,407S

E2 interaction site
3,404Y, 3,407S, 3,408L, 3,410W, 3,411M, 3,414N, 3,420I, 3,422E, 3,426I, 3,427E, 3,429D, 3,434T, 3,439L, 3,456Y, 3,460V
CDD
cd00078 (HECTc)

3,408L

E2 interaction site
3,404Y, 3,407S, 3,408L, 3,410W, 3,411M, 3,414N, 3,420I, 3,422E, 3,426I, 3,427E, 3,429D, 3,434T, 3,439L, 3,456Y, 3,460V
CDD
cd00078 (HECTc)

3,410W

E2 interaction site
3,404Y, 3,407S, 3,408L, 3,410W, 3,411M, 3,414N, 3,420I, 3,422E, 3,426I, 3,427E, 3,429D, 3,434T, 3,439L, 3,456Y, 3,460V
CDD
cd00078 (HECTc)

3,411M

E2 interaction site
3,404Y, 3,407S, 3,408L, 3,410W, 3,411M, 3,414N, 3,420I, 3,422E, 3,426I, 3,427E, 3,429D, 3,434T, 3,439L, 3,456Y, 3,460V
CDD
cd00078 (HECTc)

3,414N

E2 interaction site
3,404Y, 3,407S, 3,408L, 3,410W, 3,411M, 3,414N, 3,420I, 3,422E, 3,426I, 3,427E, 3,429D, 3,434T, 3,439L, 3,456Y, 3,460V
CDD
cd00078 (HECTc)

3,420I

E2 interaction site
3,404Y, 3,407S, 3,408L, 3,410W, 3,411M, 3,414N, 3,420I, 3,422E, 3,426I, 3,427E, 3,429D, 3,434T, 3,439L, 3,456Y, 3,460V
CDD
cd00078 (HECTc)

3,422E

E2 interaction site
3,404Y, 3,407S, 3,408L, 3,410W, 3,411M, 3,414N, 3,420I, 3,422E, 3,426I, 3,427E, 3,429D, 3,434T, 3,439L, 3,456Y, 3,460V
CDD
cd00078 (HECTc)

3,426I

E2 interaction site
3,404Y, 3,407S, 3,408L, 3,410W, 3,411M, 3,414N, 3,420I, 3,422E, 3,426I, 3,427E, 3,429D, 3,434T, 3,439L, 3,456Y, 3,460V
CDD
cd00078 (HECTc)

3,427E

E2 interaction site
3,404Y, 3,407S, 3,408L, 3,410W, 3,411M, 3,414N, 3,420I, 3,422E, 3,426I, 3,427E, 3,429D, 3,434T, 3,439L, 3,456Y, 3,460V
CDD
cd00078 (HECTc)

3,429D

E2 interaction site
3,404Y, 3,407S, 3,408L, 3,410W, 3,411M, 3,414N, 3,420I, 3,422E, 3,426I, 3,427E, 3,429D, 3,434T, 3,439L, 3,456Y, 3,460V
CDD
cd00078 (HECTc)

3,434T

E2 interaction site
3,404Y, 3,407S, 3,408L, 3,410W, 3,411M, 3,414N, 3,420I, 3,422E, 3,426I, 3,427E, 3,429D, 3,434T, 3,439L, 3,456Y, 3,460V
CDD
cd00078 (HECTc)

3,439L

E2 interaction site
3,404Y, 3,407S, 3,408L, 3,410W, 3,411M, 3,414N, 3,420I, 3,422E, 3,426I, 3,427E, 3,429D, 3,434T, 3,439L, 3,456Y, 3,460V
CDD
cd00078 (HECTc)

3,456Y

E2 interaction site
3,404Y, 3,407S, 3,408L, 3,410W, 3,411M, 3,414N, 3,420I, 3,422E, 3,426I, 3,427E, 3,429D, 3,434T, 3,439L, 3,456Y, 3,460V
CDD
cd00078 (HECTc)

3,460V

E2 interaction site
3,404Y, 3,407S, 3,408L, 3,410W, 3,411M, 3,414N, 3,420I, 3,422E, 3,426I, 3,427E, 3,429D, 3,434T, 3,439L, 3,456Y, 3,460V
CDD
cd00078 (HECTc)

Protein sequence

>MIA_00954_1
MQIKKSANIARRLAEMPPDLKNLVNSLLKMPENELPEHIDAFCSGWNRPRGDLCHWIPVLNHMDTILEKLVKKYGLDQEE
ISPKLFSPEDERLVTSILNFSTFLLDKSSNRGVYNSEAYVNNLTLSISPEVTSAALKLSGRLAERNVHSRGSRNILQHFD
ILRLLKFAVCVTSRTGDSSSSADDTILSYLNNTDVPSGFSLQYYNSDSQSTTKSSNTSKSLQPTPVEPTHSHGSTHHKTN
KISSSSSLKSKPQASRAPINREGLTEYTISTKDAVERPLDLVIADAFKVVPKNYWLDVVIKAQIAKSTANTEEGARLRSL
MIQMQCNALTVISTAIVNVNENIYESKIMNDHPNLIKQLAEILVPENPFDIHVRNAAIDSFCALCCQPAAVPEITINLSS
NVSYGTLMHVMRNILKDTRSGKKLDDEYYFFTDGMISLSTQLMMTMQPNAAASTTGGGMVPLFWELVKEEKAGPRLRSNA
LDGLMRIISEYIPGYLNTFLQQENGGQILMKILEETVESALDPSQSGSPPTLCAVDYSVNYHTIQWLKVILHFIVSITSQ
ARTAERIQQVLDSSFLTFAPKIISNPKAFGFRIISLTLGIIGSLLENESSTFGIMTENQSIDKILAEIPNLLEFSSGLHG
PVAKFLSSIAHNETGLSLIKEKDIFNEYFKLISKNLGEKDSLKHLGICLDQICTEHSSLRPIVIREAIKLIDIIIGKLNT
HDSHLQFFYELDTTSEGLEEDIPLAKRYFFLTSTIYFIEHLLKNHFSRVEFIKQRGVERILDIFESPALSFDFAFNGPAT
SLNKTVKQLFDLDIDTSYIIDALLSKMASIVDKAEAKAKLLKSLTGNEMEEDFIDFIKSLGPVENILQCFLFTVFTNYGT
GYRISDVLKAIAGEKNEKKNETDSSSFDYKDLIHRIGALHLHITWESARVRNFISLEVSDATMSLPYDPVLNSYFRSYQD
SSEYKKLKEAEAKLPEDYVRSEKYRIIKASRFVLSSSSTLINESLGTISFFLSSILPSPASDKSAYKLLDTITKSYCDQF
SLYKERQSYGAISAWFACFALRSLKDVLMANNSSNGTTFKLLLGSLIFFKQNGGIMLLSDIAQSLFNEPLISDTNSGYSM
AFIQIILHFSMMINWKPGHLVDTERAWGTSDFDFPNYFNPHLFFLEVSLTIFDCVSKIWESDSLEDKPYFITIPIILLLS
NLLHTANETYPMEGHRAAPHYISWREAPPSETMYKTIMENFCIDEADNEHISEFMAAALNHVSDVAMEFDLNADELFAKL
REAKFDNGDGILTVPRKKDGSLLMYPSDLKEMCEKIQKNAVDKVLGIIRVHPKTVYAACNFIDQIITSSNRSKPNKSSPP
QVKTSAIKELILMIISIDPSSDEDEKPLANFCHLLGILLFDTNVLFACLPDLLELIDNFVNLVETPDAVKRDWFPYVLLI
LETIFLVKEIPDAVDKIQYLPRSVAGMTDVIPKTDALPKEIYKRVFNILITVDTYKDELTVVSISRLLVFFTRDHECASL
LVQSPVFPKLLQQMKRFSTPPKEDEVENKLLVPHNPRFFKSMKEHFRITLLFILRNAVETPEIVKNIMVTDIKNGIMRHR
SQLELFFQSKIMYAARSPQIFLEVLAENCVITDLNNLQKTQFITLQEVVDTAYTKLEKSYDELSVTLDKAIAENESLGIT
TSFSDSKTSNETNDLESDVSMGEAEHFITPKPTSETTSDDKALKPTSSNIPGISSTSAFKSLTLENPSGVINILLHELFS
IGRENLFTVSVKTPEALQAWIKDHPKPEKSKENKKSSDNPYFHYACFLLQAIAELVSSYSVCKLEFINFSKKPLNASSTS
LNSTTSGSTIAPGPVPFKPRHTALNFFLQELIPIGVLESSSCVPYYEWSTISSFANLCILTLISATDEKYSESVDETQGE
PTLQFVRKFTFDTLAKAFRDASQSANDTLLWRYSKIYALADLCNRMFSSGPDRNIVSSNFSPHTVPIEDGLAICKVAFDK
DLQTVMTNSLAEIDLNYPHCRKPLKLLVKCLNKLARLVIDVFDDGSAPEKPEEEDLFASFGDISSDSEVEDEMPAIFRNS
ALSMYEAGDDLSDDGSDASGVHDDDDEMFDEDMDDSDDDLGSGADEHENIDDEEIIVDDGDRRVELLVGHNHNHNHNHDN
YNDDDDVLVLNELTMNDANSDAHIADSNERTPFVSNNSTNNNTTNHTGRGRRAESEEAPGSGYDNDSGWESFEEGDESDD
EIERLETLIQQRDFTGLDDNDGSWVEEGPMDDVDLDDIFDSHDRPIPFSGESDSQFNQHFRMLRQRNGLNFLFGGNSAGF
LNSRHPQNDISFHIPIFGNLDSLRAEADIFSNNDNNKNLEILAKRTIFRWGEAANLFWFFDKTSIVYRYLGHFINSVGDT
SIKKYADLNSLKTKIKEKEEKITLAYREKAKHEQEVKEAKEREERERKEREEREELERIQREERERAEREERERQLTSST
DDKMETSSVDMKDASEEDDEAEGSSNQTGETSGERYMMEIGGHEVDISGLGIDHTFLEAIPADMREEVLTQHLRGMQESA
SEGDTRVGELVSTFLNALPSNVRRELMNEEATEPPVALSNASVDTFDRATFFASLDPEFRRTILIEQDDETLDSLPPELA
AEGRMFRTQGFSRPEVPANFPLSENPNTQNIFRHLFSDQNSHFSNRILSTNIFNSSRPSIDEKKAKKKTISAASQNLIDK
QGVAALLRTLYLPQTHFFDTLNELLFNLCTENKANRLDIINTVLHILLDASIDKATLDRGFLQTSNRARYAQTTSKPGTP
TGTGTPGRVPIGYNVLAQEITPSTVAKQALEVLEFISHKGGPVRYFFVSEHDVPLTRRPNRKGKGRLKDRELAKEHKYPI
NILITLLGRPAIYENPITLEQLTVVLVEITKVVPILLRTVEDDKKATDSGKKESVEPALASIINDNVESSSTTGQEISTE
HKSPQKSSKSEGTESKEANTTKPHRVHEPPYIPEKNYKLISSTLVLKECSSRTFEQMLTVMKNLSSINGVKKLFAKELVN
LAVSFGPRIIESLGYLNKAVEETKPGDEVPGPILAPFSSGSSDQARLLRALAALDYLLDHSKEKEKNSSNMDIDLADDDY
INTVYKNMNFGPLWAALSESLTLIQKNSSLLYVATALLPLIESLMVICKHSAVKKISVRESNATGTNTQFTPSSKIDISN
APLETLFFIFTDQHRKILNQLVRANTKLMNGSFSILVKNPKSLEFDNKRRYFTKRLYDNSSIFSSPLNLNVQRDTAFLDS
YRTLHFRSAEEIKHARLVIKFKGEEGVDAGGLTREWYQVMSRQMFNPDYALFQPVASDRTTFHPNRTSSINDNHLYYFKF
IGRIIGKAICDNKLLDCHFSRAMYKRILGKHVSLKDMENLDPDYYKSLVWMLENDITDIITETFSIEADDYGETNIIDLK
PDGRNIPVTEENKAEYVRLVCEYRLIESVSEQLNHFLEGFHDIIPKDIVSIFDEQELELLISGLPDIDIDDWRNNTEYQN
YSPSSPQIKWFWRAVRSMDPEERAKLLQFVTGTSKVPLNGFKELVGMHGVSKFSIHRAFGSKDRLPSSHTCFNQIDLPMY
DSYENLREALLKAITEGSDSFEFA

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0004842 ubiquitin-protein transferase activity
GO:0005488 binding

Cellular Component

None predicted.

Filter view on

Entry type

Status

Per-residue features

MIA_00954_1

Protein function

Protein alignments

Mitochondrial localization by mitoprotII

Protein family membership

Domains and repeats

Detailed signature matches

Residue annotation

Protein sequence

GO term prediction

Biological Process

Molecular Function

Cellular Component