MCA_00580_1

Protein

Length

3,647 amino acids

Gene name: TOM1

Description: E3 ubiquitin-protein ligase TOM1

Browser: contigA:1782843-1793947-

RNA-seq: read pairs 6904, FPKM 23.4, percentile rank 45.7% (100% = highest expression)

Protein function

Annotation:		TOM1	E3 ubiquitin-protein ligase TOM1
KEGG:	K10592	HUWE1	E3 ubiquitin-protein ligase HUWE1 [EC:2.3.2.26]
EGGNOG:	0PHS9	PGUG_02920	Ubiquitin-protein ligase
SGD closest match:	S000002865	TOM1	E3 ubiquitin-protein ligase TOM1
CGD closest match:	CAL0000176024	TOM1	E3 ubiquitin-protein ligase

Protein alignments

	%id	Aln length
MIA_00954_1	49.84%	2149	MIA_00954_1
A0A0J9XGS3_GEOCN	35.55%	2197	Similar to Saccharomyces cerevisiae YDR457W TOM1 E3 ubiquitin ligase of the hect-domain class OS=Geotrichum candidum GN=BN980_GECA16s00373g PE=4 SV=1
UniRef50_A0A0J9XGS3	35.55%	2197	Similar to Saccharomyces cerevisiae YDR457W TOM1 E3 ubiquitin ligase of the hect-domain class n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9XGS3_GEOCN
A0A170QY39_9ASCO	50.29%	1195	E3 ubiquitin-protein ligase TOM1 OS=Sugiyamaella lignohabitans GN=TOM1 PE=4 SV=1
A0A1E3PGK5_9ASCO	46.69%	1148	Uncharacterized protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_52972 PE=4 SV=1
Q6CFL1_YARLI	47.00%	1134	YALI0B05940p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_B05940g PE=4 SV=2
A0A060SYY9_BLAAD	29.62%	2157	ARAD1C01144p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C01144g PE=4 SV=1
TOM1_YEAST	46.51%	903	E3 ubiquitin-protein ligase TOM1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TOM1 PE=1 SV=1
A0A1D8PGT3_CANAL	66.07%	504	E3 ubiquitin-protein ligase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=TOM1 PE=4 SV=1
A0A1E4TJX7_9ASCO	54.27%	656	Uncharacterized protein (Fragment) OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_14432 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.3731
Predicted cleavage: 21

Protein family membership

None predicted.

Domains and repeats

Domain
14 - 1,970
Armadillo-type fold (IPR016024)
Armadillo-like helical (IPR011989)
2,442 - 2,951
Armadillo-type fold (IPR016024)
Armadillo-like helical (IPR011989)
3,285 - 3,647
HECT domain (IPR000569)
Domain
90 - 440
E3 ubiquitin ligase, domain of unknown function DUF908 (IPR010309)
504 - 744
E3 ubiquitin ligase, domain of unknown function DUF913 (IPR010314)
2,560 - 2,658
Domain of unknown function DUF4414 (IPR025527)

1 500 1000 1500 2000 2500 3000 3647

Detailed signature matches

IPR016024

Armadillo-type fold
1. SSF48371 (ARM repeat)
  
  82 - 145
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
  
  565 - 740
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
  
  350 - 520
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
  
  2,849 - 2,886
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
  
  2,722 - 2,799
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
  
  2,926 - 2,947
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
  
  2,469 - 2,533
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
IPR011989

Armadillo-like helical
1. G3DSA:1.25.10.10
  
  14 - 1,970
  
  GENE3D
  G3DSA:1.25.10.10 (G3DSA:1.25.10.10)
  
  2,442 - 2,951
  
  GENE3D
  G3DSA:1.25.10.10 (G3DSA:1.25.10.10)
IPR010309

E3 ubiquitin ligase, domain of unknown function DUF908
1. PF06012 (DUF908)
  
  90 - 440
  
  Pfam
  PF06012 (DUF908)
IPR010314

E3 ubiquitin ligase, domain of unknown function DUF913
1. PF06025 (DUF913)
  
  504 - 744
  
  Pfam
  PF06025 (DUF913)
IPR025527

Domain of unknown function DUF4414
1. PF14377 (DUF4414)
  
  2,560 - 2,658
  
  Pfam
  PF14377 (DUF4414)
IPR000569

HECT domain
1. PF00632 (HECT)
  
  3,342 - 3,646
  
  Pfam
  PF00632 (HECT)
2. cd00078 (HECTc)
  
  3,290 - 3,645
  
  CDD
  cd00078 (HECTc)
3. SSF56204 (Hect, E3 ...)
  
  3,285 - 3,640
  
  SUPERFAMILY
  SSF56204 (Hect, E3 ligase catalytic domain)
4. SM00119 (hect_3)
  
  3,309 - 3,647
  
  SMART
  SM00119 (hect_3)
5. PS50237 (HECT)
  
  3,311 - 3,647
  
  PROSITE profiles
  PS50237 (HECT)

no IPR

Unintegrated signatures

Coil

1,668 - 1,705

COILS
Coil (Coil)

2,947 - 2,974

COILS
Coil (Coil)

2,437 - 2,509

COILS
Coil (Coil)

3,646 - 3,647

COILS
Coil (Coil)
G3DSA:3.30.24...

3,528 - 3,647

GENE3D
G3DSA:3.30.2410.10 (G3DSA:3.30.2410.10)
G3DSA:3.90.17...

3,265 - 3,446

GENE3D
G3DSA:3.90.1750.10 (G3DSA:3.90.1750.10)
mobidb-lite (disord...)

2,465 - 2,544

MobiDB Lite
mobidb-lite (disorder_prediction)

2,615 - 2,640

MobiDB Lite
mobidb-lite (disorder_prediction)

211 - 287

MobiDB Lite
mobidb-lite (disorder_prediction)

2,164 - 2,264

MobiDB Lite
mobidb-lite (disorder_prediction)

1,689 - 1,762

MobiDB Lite
mobidb-lite (disorder_prediction)

2,953 - 3,009

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

catalytic cleft cd...

3,296R

catalytic cleft
3,296R, 3,327E, 3,338E, 3,399D, 3,572F, 3,608L, 3,609P, 3,612H, 3,613T, 3,614C, 3,615F, 3,616N, 3,636A, 3,644F
CDD
cd00078 (HECTc)

3,327E

catalytic cleft
3,296R, 3,327E, 3,338E, 3,399D, 3,572F, 3,608L, 3,609P, 3,612H, 3,613T, 3,614C, 3,615F, 3,616N, 3,636A, 3,644F
CDD
cd00078 (HECTc)

3,338E

catalytic cleft
3,296R, 3,327E, 3,338E, 3,399D, 3,572F, 3,608L, 3,609P, 3,612H, 3,613T, 3,614C, 3,615F, 3,616N, 3,636A, 3,644F
CDD
cd00078 (HECTc)

3,399D

catalytic cleft
3,296R, 3,327E, 3,338E, 3,399D, 3,572F, 3,608L, 3,609P, 3,612H, 3,613T, 3,614C, 3,615F, 3,616N, 3,636A, 3,644F
CDD
cd00078 (HECTc)

3,572F

catalytic cleft
3,296R, 3,327E, 3,338E, 3,399D, 3,572F, 3,608L, 3,609P, 3,612H, 3,613T, 3,614C, 3,615F, 3,616N, 3,636A, 3,644F
CDD
cd00078 (HECTc)

3,608L

catalytic cleft
3,296R, 3,327E, 3,338E, 3,399D, 3,572F, 3,608L, 3,609P, 3,612H, 3,613T, 3,614C, 3,615F, 3,616N, 3,636A, 3,644F
CDD
cd00078 (HECTc)

3,609P

catalytic cleft
3,296R, 3,327E, 3,338E, 3,399D, 3,572F, 3,608L, 3,609P, 3,612H, 3,613T, 3,614C, 3,615F, 3,616N, 3,636A, 3,644F
CDD
cd00078 (HECTc)

3,612H

catalytic cleft
3,296R, 3,327E, 3,338E, 3,399D, 3,572F, 3,608L, 3,609P, 3,612H, 3,613T, 3,614C, 3,615F, 3,616N, 3,636A, 3,644F
CDD
cd00078 (HECTc)

3,613T

catalytic cleft
3,296R, 3,327E, 3,338E, 3,399D, 3,572F, 3,608L, 3,609P, 3,612H, 3,613T, 3,614C, 3,615F, 3,616N, 3,636A, 3,644F
CDD
cd00078 (HECTc)

3,614C

catalytic cleft
3,296R, 3,327E, 3,338E, 3,399D, 3,572F, 3,608L, 3,609P, 3,612H, 3,613T, 3,614C, 3,615F, 3,616N, 3,636A, 3,644F
CDD
cd00078 (HECTc)

3,615F

catalytic cleft
3,296R, 3,327E, 3,338E, 3,399D, 3,572F, 3,608L, 3,609P, 3,612H, 3,613T, 3,614C, 3,615F, 3,616N, 3,636A, 3,644F
CDD
cd00078 (HECTc)

3,616N

catalytic cleft
3,296R, 3,327E, 3,338E, 3,399D, 3,572F, 3,608L, 3,609P, 3,612H, 3,613T, 3,614C, 3,615F, 3,616N, 3,636A, 3,644F
CDD
cd00078 (HECTc)

3,636A

catalytic cleft
3,296R, 3,327E, 3,338E, 3,399D, 3,572F, 3,608L, 3,609P, 3,612H, 3,613T, 3,614C, 3,615F, 3,616N, 3,636A, 3,644F
CDD
cd00078 (HECTc)

3,644F

catalytic cleft
3,296R, 3,327E, 3,338E, 3,399D, 3,572F, 3,608L, 3,609P, 3,612H, 3,613T, 3,614C, 3,615F, 3,616N, 3,636A, 3,644F
CDD
cd00078 (HECTc)
E2 interaction sit...

3,427Y

E2 interaction site
3,427Y, 3,430S, 3,431L, 3,433W, 3,434I, 3,437N, 3,443I, 3,445E, 3,449I, 3,450D, 3,452D, 3,457R, 3,462L, 3,479Y, 3,483V
CDD
cd00078 (HECTc)

3,430S

E2 interaction site
3,427Y, 3,430S, 3,431L, 3,433W, 3,434I, 3,437N, 3,443I, 3,445E, 3,449I, 3,450D, 3,452D, 3,457R, 3,462L, 3,479Y, 3,483V
CDD
cd00078 (HECTc)

3,431L

E2 interaction site
3,427Y, 3,430S, 3,431L, 3,433W, 3,434I, 3,437N, 3,443I, 3,445E, 3,449I, 3,450D, 3,452D, 3,457R, 3,462L, 3,479Y, 3,483V
CDD
cd00078 (HECTc)

3,433W

E2 interaction site
3,427Y, 3,430S, 3,431L, 3,433W, 3,434I, 3,437N, 3,443I, 3,445E, 3,449I, 3,450D, 3,452D, 3,457R, 3,462L, 3,479Y, 3,483V
CDD
cd00078 (HECTc)

3,434I

E2 interaction site
3,427Y, 3,430S, 3,431L, 3,433W, 3,434I, 3,437N, 3,443I, 3,445E, 3,449I, 3,450D, 3,452D, 3,457R, 3,462L, 3,479Y, 3,483V
CDD
cd00078 (HECTc)

3,437N

E2 interaction site
3,427Y, 3,430S, 3,431L, 3,433W, 3,434I, 3,437N, 3,443I, 3,445E, 3,449I, 3,450D, 3,452D, 3,457R, 3,462L, 3,479Y, 3,483V
CDD
cd00078 (HECTc)

3,443I

E2 interaction site
3,427Y, 3,430S, 3,431L, 3,433W, 3,434I, 3,437N, 3,443I, 3,445E, 3,449I, 3,450D, 3,452D, 3,457R, 3,462L, 3,479Y, 3,483V
CDD
cd00078 (HECTc)

3,445E

E2 interaction site
3,427Y, 3,430S, 3,431L, 3,433W, 3,434I, 3,437N, 3,443I, 3,445E, 3,449I, 3,450D, 3,452D, 3,457R, 3,462L, 3,479Y, 3,483V
CDD
cd00078 (HECTc)

3,449I

E2 interaction site
3,427Y, 3,430S, 3,431L, 3,433W, 3,434I, 3,437N, 3,443I, 3,445E, 3,449I, 3,450D, 3,452D, 3,457R, 3,462L, 3,479Y, 3,483V
CDD
cd00078 (HECTc)

3,450D

E2 interaction site
3,427Y, 3,430S, 3,431L, 3,433W, 3,434I, 3,437N, 3,443I, 3,445E, 3,449I, 3,450D, 3,452D, 3,457R, 3,462L, 3,479Y, 3,483V
CDD
cd00078 (HECTc)

3,452D

E2 interaction site
3,427Y, 3,430S, 3,431L, 3,433W, 3,434I, 3,437N, 3,443I, 3,445E, 3,449I, 3,450D, 3,452D, 3,457R, 3,462L, 3,479Y, 3,483V
CDD
cd00078 (HECTc)

3,457R

E2 interaction site
3,427Y, 3,430S, 3,431L, 3,433W, 3,434I, 3,437N, 3,443I, 3,445E, 3,449I, 3,450D, 3,452D, 3,457R, 3,462L, 3,479Y, 3,483V
CDD
cd00078 (HECTc)

3,462L

E2 interaction site
3,427Y, 3,430S, 3,431L, 3,433W, 3,434I, 3,437N, 3,443I, 3,445E, 3,449I, 3,450D, 3,452D, 3,457R, 3,462L, 3,479Y, 3,483V
CDD
cd00078 (HECTc)

3,479Y

E2 interaction site
3,427Y, 3,430S, 3,431L, 3,433W, 3,434I, 3,437N, 3,443I, 3,445E, 3,449I, 3,450D, 3,452D, 3,457R, 3,462L, 3,479Y, 3,483V
CDD
cd00078 (HECTc)

3,483V

E2 interaction site
3,427Y, 3,430S, 3,431L, 3,433W, 3,434I, 3,437N, 3,443I, 3,445E, 3,449I, 3,450D, 3,452D, 3,457R, 3,462L, 3,479Y, 3,483V
CDD
cd00078 (HECTc)

Protein sequence

>MCA_00580_1
MQIKISANISRRLTEMPADLKQLVNDMTTMPEDELPTPLNEFCQGYSRPRGDLCHWIPLLNRLDLILERTVKKYNLENDE
ISPIPFSKEDERLITSILDFITYLLDKSSNRGIFNSEKYVNNLVYSISPEITRSALKLLVRLAERNLQAKTARNSLSYAD
TVKLLQFATCVTARYGDQTSSNDKTFLDFYNNANPPSAVSLQYYSVGKPNDKSNKSFTTPKAQHLASASTPGVSDTPTRA
PQKPSSSSSSSSTPTSRKVRKATSTSSLKSRHNYSATPSKTNSGSSNSVGLTEYTFSVKDAISRPLVDVVKDAFKALPKN
VWLEALIKAMIAQSTANTKEGARLRYLLIQMQCSALTVIAYTVSNQTIFESKIMTEYPHLLKQLIELIVPENNFPIKIKG
CAVDTFCALSFQPSALPDLTVLLSSNVSYGTLVHIMRDILKQIRSGKKFSEDDYYFIDGMISIALQLGASMQPNSMASTA
NGIIPLFWEFASEKATDPRISSNFMDALMRFFTEVFDPNMFLQDEKESQRLIDMLEYATGLALDPATAGERPKLCSVDYT
ISYHIIQWIKVILHFILAITGSARTNERIPKVLDSSFLTLTPKIISNPKIFGFRVISLTLSIIGNLLKNDVSTFGIMTEN
KTMNEILACLPNLLEFSSGLHGSVAKFLSSIAHNDAGLKIIKEEGIFNQYFDIVSKNFGDKENLRHLGVCLDQICTEHAT
LRPVVIKETIKLIEIVMKQLSEYNFPTQFFYELDSASTESEEENLLAKRYFFLNSAVSFVDSLLFNHISRVEIIKQNGVD
QVISLLESPALSYDFAFCSTATSAFKSIKQLFDLDINQVEVMDTILIRISNVVQTVEDKIAKLPTLDTDIIMTDEYMDFI
KSLGPLQNYLQCFLFTVFTNYGTGYSISRVLKALTDLRLEDNLTEEEKERALKYKDLLPRLGSVQRRIMWEAERIKNHIS
KEILEATNPLPYDPVLNSYFHRYQDSEDFKKLKELEEKLPEDVSTSKSYRIIKASRFILGSSNSAITETFSLISSFIPRA
FPHSISDKMAYKLLNTICEVYYNHFTLFEKNKEFPKITASFVESALKSLETFFSTGPSRIVTYSQGPVIFFKQLGGIQLL
AKIAKDLFNNPESTNEESPESRSLAQILKLFNSLATWKLITRSDSSRSWGQLNPALPNYFIFSYFFLEVRITIYNTIIEL
WDSDELLERSSPVRKGFLTLVSNIFMVLNEDYPQSQRKRPLSISWEEAPPSETMREIIIKNFDLDESEVELVDSALLDNK
NSISAVASALDLEESEIKKYINADTNYKQAETLTVPESADGKPLMYPEDFKKMRIHVLENCLDKIFKIVGKDSDCVFTAC
NFLLYIIPSTYQSAKSKHDLPLLKDSVNQLLLEIVSIDATDKEQTTKLSSYCHLLGILLFDNAVLSSSLTNIIDFVEMFV
GMLEIPEAPKCEWFPYVLLLLETIFAIKEVPDAMENAPQIKKLMNPTTSIPSTEPIAAEVYDRVFNILTSIDDFHDEMVA
LAASRLLVFFTRKHERAMELLHSSNFVKILQLVKRFSTQKDQPVPNKKLQLPKDYTKNITEQFRMALFIILRNTVETPEI
VKSIMKRSINQRFSSRAGNEEIFNFMRNNQALAARSPDLFLEVLGEICIITNPAHISNPSKSTLVSLKSTADSQIEALKT
TNQRLAEKRDKIKDLESTEKADEDTEISSATPLIPTNDGNDSDVSMEDVASFPESHPETNVSVQTPAKQTDSSTTQTPGT
SFKSIVYQNPSGVINILLQELMSIDNLFTVPIKTEEALTNWIKENPKPEKSKDVKKESENPSFYYACFLLQAIAELVASY
NVCKLEFINFSKRSLFGGSTTSLASTSSLSTITPSQNQFKPRHTAINFFLQELMPLGVLESSNAIPYFEWSQVSSLAFLC
VMNLLSATDEKISTSIDETQSEPTLVFVRKFIIEAIAKAFKEASHSTNDTISWRYSKITAYADLCHRLFSNNSSRVSTGS
HFPLHSVNVADGAAIAKVAYEKGFANVLTNSLSEIDLNFPLCRKPIKLLVKCLGKLARLTMEIPDNGVIEKVEDEDFEFS
DSGAETPAIIRNSALSMFDANDDLSDEVEDFDVSEGDHDSVMEEDEELEELDYMDDGAQSDVGSSSAMDDQLDHEEGDMI
SEGSEMLASDEDSNESDLDEGDYEEVEIIVEHSHSEDDGDDEQSEDDSIDQDDEDDANRMHIDLDDNASFQGSHPPTNEN
EPFVAGSSDSDWESFEDDEDVDDDELERVETMLQQQDLDGLHDHGSGWSDRERELHEQVFSLDDLTFQRDISQDFDDDDD
YDDYLNPSYSNDRVRRPPTVMNPLLANNPDYFGISGSSHPFNSQISRSRNLIHQAFGVIGSGFDSLFNDHDIESFKIRAS
RTLPRWNEASFLFRVFGNNDVMYRFIPYFIASVSDYSKKICDEIDQLQAQKKEIEEEISQIQKEIDKREREKKEREEKER
KEKEEAERKKKEEEEAAKKRAEEAEQTKTAPVEDIPDSESVEMVDQDEPSSSSNAPAPTIPDEYNVRLNGHDVNISALGI
DPTFLEAIPEDMLEEVVTQRFRELQELSGQGDEEAEASVSRFVSTLPAAVRADLLNEESDDRDDDDEGHTGPAELDNASF
FASLDPDLRRDVLLEQDEETLLTLPPDLVAEARTVRHRNYTRPNVALSPNPFGADTISRVLNLPPSLLSYDNIMSVATNS
SLPILSPTTTKEPPKKKKVSAAYLHIIDKQGIAALLRTLYIPQPSPQESLLQLLYNLCTENKANRLDIINSLLHILADAS
VDKATLDRGFLHVSNRARYTVTPSKSQSLLDTPGPVTPGYNVLSQEVIPSIVAKQALEVLQFISHKSGPVKYFFVSEHDI
PLTRKLSKKGKGKDKEVSKENKYPINLLISLLGKPSIYESGSTLEQLTAVLVEITKVVPVLLKSIHDDKKKKNLEDLDKE
NEESKDESKKEEDSSKETAESSDPATTSTEIDTKEDSSKAKSDTQTKPYTAPYIPDKKYMLISSTLVLKECNSRTFEQML
TVMRNLSAIEGVKHLFARELVSKAVSFGPRIIGNLEKLLKALKELKPDDELPVSVLAPFSSGSSDQARLLRALAALDYLY
APTKEKPYDDKKKSEEKTEEEDHLNKVYSDMNFGPLWSALSDVLSAIQDNASLGYVSTALLPLIESLMVVCKHSAVKQIS
LRENNSTGGAHPFVPSTKIDISKASLENLFFAFTDQHRKILNQLVRANPKLMNGSFSILVKNPKSLEFDNKRRYFTGKLY
GNEMPHTAPLNVNVRREQTFLDSYRILYYKSPEEIKRSRLSIKFKGEEGIDAGGLTREWYQVMSRQMFNPDYALFQPVAA
DRTTFHPNRTSGVNEDHLSYFKFVGRIIGKAIYDNKLLDCHFSRAMYKKILGKPVSLKDMENLDLDYYKSLQWILENDIT
DIITETFSIDTDDYGERKVIDLKPDGHNIPVTEENKAEYVRLVCEYRLIESVRSQLEHFLEGFHEIIPKDLVSIFDEQEL
ELLISGLPDIDIDDWRNNTQYQNYSPSSIQIKWFWRAVRAMDAEERAKLLQFVTGTSKVPLNGFKELVGMDGVSKFSIHR
AYGSNDRLPSSHTCFNQIDLPAYDSYEMLREALLKAITEGGESFELA

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0004842 ubiquitin-protein transferase activity
GO:0005488 binding

Cellular Component

None predicted.

Filter view on

Entry type

Status

Per-residue features

MCA_00580_1

Protein function

Protein alignments

Mitochondrial localization by mitoprotII

Protein family membership

Domains and repeats

Detailed signature matches

Residue annotation

Protein sequence

GO term prediction

Biological Process

Molecular Function

Cellular Component