Protein

MIA_00818_1

Length
944 amino acids

Browser: contig01:2315155-2317990+

Protein function

EGGNOG:0PHW1NAM2Leucyl-tRNA synthetase
SGD closest match:S000004374NAM2Leucine--tRNA ligase, mitochondrial
CGD closest match:CAL0000195709NAM2Leucine--tRNA ligase

Protein alignments

%idAln lengthE-value
A0A0J9XFB9_GEOCN64.437%8970.0Similar to Saccharomyces cerevisiae YLR382C NAM2 Mitochondrial leucyl-tRNA synthetase OS=Geotrichum candidum GN=BN980_GECA13s02617g PE=3 SV=1
UniRef50_A0A0J9XFB964.437%8970.0Similar to Saccharomyces cerevisiae YLR382C NAM2 Mitochondrial leucyl-tRNA synthetase n=4 Tax=saccharomyceta TaxID=716545 RepID=A0A0J9XFB9_GEOCN
MCA_06373_162.665%9080.0MCA_06373_1
A0A167BZV4_9ASCO56.908%9120.0Leucine--tRNA ligase NAM2 OS=Sugiyamaella lignohabitans GN=NAM2 PE=3 SV=1
A0A060T578_BLAAD58.237%8620.0ARAD1C09834p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C09834g PE=3 SV=1
Q6C363_YARLI52.674%8600.0YALI0F02299p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_F02299g PE=3 SV=1
SYLM_YEAST50.640%8590.0Leucine--tRNA ligase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=NAM2 PE=1 SV=1
A0A1D8PQ56_CANAL52.133%8440.0Leucine--tRNA ligase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=NAM2 PE=3 SV=1
A0A1E4TL37_9ASCO44.371%9060.0Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_22053 PE=3 SV=1
A0A1E3PFH9_9ASCO52.152%6040.0YLR382Cp-like protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_84018 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.9902
Predicted cleavage: 16

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
  3. Domain
1 100 200 300 400 500 600 700 800 944

Detailed signature matches

  1. Family IPR002302
    Leucine-tRNA ligase
    1. TIGR00396
    2. PR00985 (TRNASYNTHLEU)
    3. MF_00049_B (Leu_tRN...)
  2. Domain IPR014729
    Rossmann-like alpha/beta/alpha sandwich fold
    1. G3DSA:3.40.50...
  3. Domain IPR015413
    Methionyl/Leucyl tRNA synthetase
    1. PF09334 (tRNA-synt_1g)
  4. Domain IPR009008
    Valyl/Leucyl/Isoleucyl-tRNA synthetase, editing domain
    1. SSF50677 (ValRS/Ile...)
  5. Domain IPR025709
    Leucyl-tRNA synthetase, editing domain
    1. PF13603 (tRNA-synt_1_2)
  6. Domain IPR002300
    Aminoacyl-tRNA synthetase, class Ia
    1. PF00133 (tRNA-synt_1)
  7. Domain IPR009080
    Aminoacyl-tRNA synthetase, class Ia, anticodon-binding
    1. G3DSA:1.10.73...
    2. SSF47323 (Anticodon...)
  8. Domain IPR013155
    Methionyl/Valyl/Leucyl/Isoleucyl-tRNA synthetase, anticodon-binding
    1. PF08264 (Anticodon_1)
  9. SITE IPR001412
    Aminoacyl-tRNA synthetase, class I, conserved site
    1. PS00178 (AA_TRNA_LI...)
Unintegrated signatures no IPR
Unintegrated signatures
  1. G3DSA:3.90.74...
  2. SSF52374 (Nucleotid...)
  3. cd00812 (LeuRS_core)

Residue annotation

  1. active site cd00812
  2. HIGH motif cd00812
  3. KMSKS motif cd00812

Protein sequence

>MIA_00818_1
MLATAHRGVVRQIRTQSALVTVASSSSSKFFKPLPSAHKFHSAGFLYHREKLSTTELDEKWLNIWKKNPSRLDTPTFVDS
KTGQPLDREKYYALSMFPYPSGMLHIGHLRVYTISDVLARYRRMNGYNVIHAMGWDAFGLPAENAAIERGIDPETWTLSN
IQKMKDQMNIMFADFDWEREIVTCSPEYYKHTQQLFLKLYKAGLAYRKEAVVNWDPIEKTVLANEQVDDEGRSWRSGAIV
EKKYLEQWFLGITKYASELLDDLSLLKEWPERVKTMQKNWIGKSQGATITFPLSTNESLTAFTTRPDTLYAVQYVALSLN
HPIVKSIAQTDKDLSDFLTNAASLPEDTKEGYKLKDIYAANPLNPSKHNLPVFVAPYVLDSYGHGSVMGCPGHDTRDYEF
WKKNMPGFSVIETVTPPSDYTPEANSPPIYTGKDGQLNEVAGPYSGMNAREGGKKIIQDLEPTNLAKFDIKWRLRDWLIS
RQRFWGAPIPIIYCDSCGTVPVPDEELPVLLPKGLNGPLSTSPEFVHTHCPSCGGEAKRDTDTMDTFMDSSWYFFRYTDP
HNTKEIFDYKKASELMPVDIYVGGIEHAILHLLYSRFISKFLAKNGNWSGGDLNGEPVRRLVTQGMVHGKTTVCPETGKF
LKPHEIDYENNDPNKPLVKTSQKPALVSYEKMSKSKYNGADPGECIKTHGADATRAHILFQAPVSDVLNWDESQIVGVKR
WLSKVSTFTTSLADAITESANKTQNASKFLNDLKDQAHAVKFDKLSKNEQKMWIEIQKLIKSITDSFHDTLSLNTVISDY
MKLTNSIQSTFGAGSFDNKSLPIALYALEKLLKVMAPVTPVNASDNWQYLQQALNPDSKNSFSVFAEAWPQEEAIPGADL
GRFSIIINGKRRFVLSASKSLAGKEKELLDLVYKSPESKEWLEGKSITKTTVSPKGFAVTIVTN

GO term prediction

Biological Process

GO:0006418 tRNA aminoacylation for protein translation
GO:0006429 leucyl-tRNA aminoacylation

Molecular Function

GO:0000166 nucleotide binding
GO:0002161 aminoacyl-tRNA editing activity
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004823 leucine-tRNA ligase activity
GO:0005524 ATP binding

Cellular Component

None predicted.