Protein

MIA_00637_1

Length
740 amino acids

Browser: contig01:1838656-1840879+

Protein function

EGGNOG:0PFC8DUS3Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Specifically modifies U47 in cytoplasmic tRNAs (By similarity)
SGD closest match:S000004393DUS3tRNA-dihydrouridine(47) synthase [NAD(P)(+)]
CGD closest match:CAL0000188545DUS3tRNA-dihydrouridine(47) synthase [NAD(P)(+)]

Protein alignments

%idAln lengthE-value
MCA_05534_172.210%4390.0MCA_05534_1
A0A0J9X925_GEOCN68.193%4150.0tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Geotrichum candidum GN=BN980_GECA05s05444g PE=3 SV=1
UniRef50_A0A0J9X92568.193%4150.0tRNA-dihydrouridine(47) synthase [NAD(P)(+)] n=2 Tax=Saccharomycetales TaxID=4892 RepID=A0A0J9X925_GEOCN
A0A060T1M5_BLAAD68.622%3920.0tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1A01452g PE=3 SV=1
A0A1E3PJ64_9ASCO65.163%3990.0tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_25930 PE=3 SV=1
A0A167D7J2_9ASCO64.563%4120.0tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Sugiyamaella lignohabitans GN=DUS3 PE=3 SV=1
DUS3_CANAL61.461%3971.17e-169tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=DUS3 PE=3 SV=2
DUS3_YARLI63.077%3901.71e-168tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=DUS3 PE=3 SV=1
A0A1E4T9R7_9ASCO61.692%4021.60e-167tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_33044 PE=3 SV=1
DUS3_YEAST55.779%3983.48e-149tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=DUS3 PE=1 SV=2

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.1792

Protein family membership

Domains and repeats

  1. Domain
1 100 200 300 400 500 600 740

Detailed signature matches

  1. Family IPR001269
    tRNA-dihydrouridine synthase
    1. PF01207 (Dus)
  2. Domain IPR000571
    Zinc finger, CCCH-type
    1. SSF90229 (CCCH zinc...)
    2. G3DSA:4.10.10...
    3. PS50103 (ZF_C3H1)
  3. Domain IPR013785
    Aldolase-type TIM barrel
    1. G3DSA:3.20.20.70
  4. Domain IPR035587
    DUS-like, FMN-binding domain
    1. cd02801 (DUS_like_FMN)
  5. SITE IPR018517
    tRNA-dihydrouridine synthase, conserved site
    1. PS01136 (UPF0034)
Unintegrated signatures no IPR
Unintegrated signatures
  1. Coil
  2. SSF51395 (FMN-linke...)
  3. mobidb-lite (disord...)

Residue annotation

  1. FMN binding site c...
  2. active site cd02801
  3. catalytic residues...
  4. substrate binding ...

Protein sequence

>MIA_00637_1
MTEDTPLDTSAAKRQKTESSAIPERINGVTPIKPEYLIIRETRASAPSVTPASLDDDAEGAGVSQRAESSGQAGDIAKNR
RERKAKRKETRGQNKGRRMVQSKDTVKFCDGINAQLVKYFGERDPNAPEDEDPQVQCAFGDSCRFEHNVATYLKSKTEDL
PGVCPVYAAIGYCPSGLRCRWLHSHYIVREGRAMGELKVDKAKYDKAVKEDYEVNRVPAQTLNSLQRKKFEFPLSNQYIK
YLDERIALNRKIFSKIVPPEAEEVKGQKEESKEPKDEAKEESKEPKEEAKEESKEEPKEESTKTESSEPPSDPTAEPVTT
SSTESAVKTAVEDKRANYIEPPFKPAEKRRINMDRKYIVSPLTTVGNLPYRRLMKTLGADVTYGEMALALPIVMGQKSEW
ALARAHSSEIGKFGVQIAAAKHWQAIKAAEALSTLTAPGALQEVNLNCGCPIDLVYRSGAGSALMDNPSRLVRILEGMNA
VSGDIPVTVKIRMGTKDERPTAIGLVPRLVKEGQVAAITIHGRSREQRYTKLADWGYIGEVAATVRQLREESADEMGGRY
VKPWIIGNGDVYSWEDWYNAVECEDETKRVDSVMVARGALIKPWIFEEIEAKQHLDKSVSQRLDLLKTYAEFGLEHWGSD
EYGVNMTRRYMCEFLSFTYRYVPVGLLAHLPPRINDRPPPWQGRDEMETMLGSSDYRDWIKLTEMYLGPAGEGFDFTPKH
RSNAYSNNNTAVQSEDAING

GO term prediction

Biological Process

GO:0008033 tRNA processing
GO:0055114 oxidation-reduction process

Molecular Function

GO:0003824 catalytic activity
GO:0017150 tRNA dihydrouridine synthase activity
GO:0046872 metal ion binding
GO:0050660 flavin adenine dinucleotide binding

Cellular Component

None predicted.