Protein

MCA_05534_1

Length
734 amino acids

Gene name: DUS3

Description: tRNA-dihydrouridine(47) synthase [NAD(P)(+)]

Browser: contigD:1572388-1574593-

RNA-seq: read pairs 1195, FPKM 20.1, percentile rank 41.5% (100% = highest expression)

Protein function

Annotation:DUS3tRNA-dihydrouridine(47) synthase [NAD(P)(+)]
KEGG:K05544DUS3 tRNA-dihydrouridine synthase 3 [EC:1.3.1.89]
EGGNOG:0PFC8DUS3Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs. Specifically modifies U47 in cytoplasmic tRNAs (By similarity)
SGD closest match:S000004393DUS3tRNA-dihydrouridine(47) synthase [NAD(P)(+)]
CGD closest match:CAL0000188545DUS3tRNA-dihydrouridine(47) synthase [NAD(P)(+)]

Protein alignments

%idAln lengthE-value
MIA_00637_156.44%7760.0MIA_00637_1
A0A167D7J2_9ASCO51.61%7130.0tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Sugiyamaella lignohabitans GN=DUS3 PE=3 SV=1
A0A0J9X925_GEOCN50.28%7240.0tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Geotrichum candidum GN=BN980_GECA05s05444g PE=3 SV=1
UniRef50_A0A0J9X92550.28%7240.0tRNA-dihydrouridine(47) synthase [NAD(P)(+)] n=2 Tax=Saccharomycetales TaxID=4892 RepID=A0A0J9X925_GEOCN
A0A060T1M5_BLAAD51.41%7080.0tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1A01452g PE=3 SV=1
A0A1E3PJ64_9ASCO51.02%6880.0tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_25930 PE=3 SV=1
DUS3_YARLI48.82%7230.0tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=DUS3 PE=3 SV=1
DUS3_CANAL47.90%7140.0tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=DUS3 PE=3 SV=2
DUS3_YEAST43.61%7200.0tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=DUS3 PE=1 SV=2
A0A1E4T9R7_9ASCO43.40%7120.0tRNA-dihydrouridine(47) synthase [NAD(P)(+)] OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_33044 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0421

Protein family membership

Domains and repeats

  1. Domain
1 100 200 300 400 500 600 734

Detailed signature matches

  1. Family IPR001269
    tRNA-dihydrouridine synthase
    1. PF01207 (Dus)
  2. Domain IPR000571
    Zinc finger, CCCH-type
    1. G3DSA:4.10.10...
  3. Domain IPR013785
    Aldolase-type TIM barrel
    1. G3DSA:3.20.20.70
  4. Domain IPR035587
    DUS-like, FMN-binding domain
    1. cd02801 (DUS_like_FMN)
  5. SITE IPR018517
    tRNA-dihydrouridine synthase, conserved site
    1. PS01136 (UPF0034)
Unintegrated signatures no IPR
Unintegrated signatures
  1. SSF51395 (FMN-linke...)
  2. mobidb-lite (disord...)

Residue annotation

  1. FMN binding site c...
  2. active site cd02801
  3. catalytic residues...
  4. substrate binding ...

Protein sequence

>MCA_05534_1
MSDKNKAESQSVDAIEPASKRVKLESSTTDSQYPDRIKGITPIKPEFLIAKVNYSGDGSDIKTEKTVDYDDDAEGEGLEA
RSGGITDGKQRRQKKEKNRGQNKNRQMIQQKERVKFCDTLNTQIIDYFNDVAMKTLKQQENAEGSEDKPVVLDLKCTYGS
KCRFEHDVENYLKLKQPDMDAKCPVWDAIGYCPSGLKCRWLSSHWTSDQIVESESTKLPGSLVINTEKLDNCLKNDLTVE
YNRVSSEILNQLQRKKYEFPLSDAYIKYLDRRVSDNRQKHSAGGPDSGSAPNENFSHPETEVKTEKEDSRAAYVEAPFLP
SEKRKLNLERKYIVSPLTTVGNLPYRRLMKTLGADVTYGEMALALPIVQGHKSEWALARCHSSEVGGFGVQITAAKHWQA
IKAAEALSKLSSARVTNAQTKDSVINEINLNSGCPIDLVYRSGAGSALMDNASRMIRILEGMNAVSGEIPVTVKIRTGTK
DNKPTAESLCKRLINESNVAAITIHGRSRQQRYTKLADWDYIGQVAKFVKEEREKRFEEEGSGRYVKPWIIGNGDVYTWE
DWYRAVEGDTEAYDNEEERKLRSVDSVMVARGALIKPWIFEEVEARQHLDKSSTERLEILKQYAEFGLEHWGSDEYGVNL
TRRYLCEFISFTHRYVPVGILSHLPPKLNDRPPAWKGRDEMETLMGSSDYKDWIKITELFLGKAGEGFEFTPKHKSNSYN
TNGSINAEDSINGK

GO term prediction

Biological Process

GO:0008033 tRNA processing
GO:0055114 oxidation-reduction process

Molecular Function

GO:0003824 catalytic activity
GO:0017150 tRNA dihydrouridine synthase activity
GO:0046872 metal ion binding
GO:0050660 flavin adenine dinucleotide binding

Cellular Component

None predicted.