MIA_00555_1

Protein

Length

1,814 amino acids

Protein function

EGGNOG:	0PGIG	UFD4	Ubiquitin-protein ligase
SGD closest match:	S000001493	UFD4	Ubiquitin fusion degradation protein 4
CGD closest match:	CAL0000196972	CAALFM_CR01170WA	Putative ubiquitin-protein ligase

Protein alignments

	%id	Aln length	E-value
A0A0J9XEP0_GEOCN	56.698%	642	0.0	Similar to saccharomyces cerevisiae YKL010C UFD4 Ubiquitin-protein ligase (E3) that interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome OS=Geotrichum candidum GN=BN980_GECA12s03145g PE=4 SV=1
UniRef50_A0A0J9XEP0	56.698%	642	0.0	Similar to saccharomyces cerevisiae YKL010C UFD4 Ubiquitin-protein ligase (E3) that interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9XEP0_GEOCN
MCA_01892_1	55.469%	640	0.0	MCA_01892_1
A0A167FFR1_9ASCO	48.239%	653	0.0	Putative ubiquitin-protein ligase UFD4 OS=Sugiyamaella lignohabitans GN=UFD4 PE=4 SV=1
A0A060T950_BLAAD	49.768%	647	0.0	ARAD1C35288p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C35288g PE=4 SV=1
A0A1E3PJY7_9ASCO	44.790%	643	9.36e-156	Uncharacterized protein (Fragment) OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_5354 PE=4 SV=1
Q6C6X9_YARLI	44.601%	639	2.70e-149	YALI0E05401p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E05401g PE=4 SV=1
Q5A892_CANAL	42.879%	660	5.31e-147	Putative ubiquitin-protein ligase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=CAALFM_CR01170WA PE=4 SV=1
UFD4_YEAST	38.318%	642	3.16e-118	Ubiquitin fusion degradation protein 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=UFD4 PE=1 SV=1
A0A1E4THX7_9ASCO	39.070%	645	2.46e-124	Uncharacterized protein (Fragment) OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_18258 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0194

Protein family membership

None predicted.

Domains and repeats

Domain
9 - 773
Armadillo-type fold (IPR016024)
Armadillo-like helical (IPR011989)
801 - 824
Armadillo-type fold (IPR016024)
1,408 - 1,814
HECT domain (IPR000569)

1 200 400 600 800 1000 1200 1400 1600 1814

Detailed signature matches

IPR016024

Armadillo-type fold
1. SSF48371 (ARM repeat)
  
  649 - 766
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
  
  801 - 824
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
  
  575 - 614
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
  
  206 - 545
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
IPR011989

Armadillo-like helical
1. G3DSA:1.25.10.10
  
  9 - 773
  
  GENE3D
  G3DSA:1.25.10.10 (G3DSA:1.25.10.10)
IPR000569

HECT domain
1. PF00632 (HECT)
  
  1,462 - 1,813
  
  Pfam
  PF00632 (HECT)
2. cd00078 (HECTc)
  
  1,413 - 1,809
  
  CDD
  cd00078 (HECTc)
3. SSF56204 (Hect, E3 ...)
  
  1,408 - 1,807
  
  SUPERFAMILY
  SSF56204 (Hect, E3 ligase catalytic domain)
4. SM00119 (hect_3)
  
  1,424 - 1,814
  
  SMART
  SM00119 (hect_3)
5. PS50237 (HECT)
  
  1,453 - 1,814
  
  PROSITE profiles
  PS50237 (HECT)

no IPR

Unintegrated signatures

Coil

1,075 - 1,105

COILS
Coil (Coil)

745 - 783

COILS
Coil (Coil)

1,580 - 1,600

COILS
Coil (Coil)

806 - 841

COILS
Coil (Coil)
G3DSA:3.30.21...

1,578 - 1,654

GENE3D
G3DSA:3.30.2160.10 (G3DSA:3.30.2160.10)
G3DSA:3.30.24...

1,692 - 1,814

GENE3D
G3DSA:3.30.2410.10 (G3DSA:3.30.2410.10)
G3DSA:3.30.70...

1,077 - 1,281

GENE3D
G3DSA:3.30.70.330 (G3DSA:3.30.70.330)
G3DSA:3.90.17...

1,377 - 1,577

GENE3D
G3DSA:3.90.1750.10 (G3DSA:3.90.1750.10)
mobidb-lite (disord...)

1,081 - 1,177

MobiDB Lite
mobidb-lite (disorder_prediction)

772 - 849

MobiDB Lite
mobidb-lite (disorder_prediction)

81 - 106

MobiDB Lite
mobidb-lite (disorder_prediction)

119 - 259

MobiDB Lite
mobidb-lite (disorder_prediction)

1,385 - 1,405

MobiDB Lite
mobidb-lite (disorder_prediction)

1 - 69

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

catalytic cleft cd...

1,419R

catalytic cleft
1,419R, 1,447D, 1,457E, 1,538D, 1,739F, 1,775L, 1,776P, 1,779M, 1,780T, 1,781C, 1,782V, 1,783N, 1,803A
CDD
cd00078 (HECTc)

1,447D

catalytic cleft
1,419R, 1,447D, 1,457E, 1,538D, 1,739F, 1,775L, 1,776P, 1,779M, 1,780T, 1,781C, 1,782V, 1,783N, 1,803A
CDD
cd00078 (HECTc)

1,457E

catalytic cleft
1,419R, 1,447D, 1,457E, 1,538D, 1,739F, 1,775L, 1,776P, 1,779M, 1,780T, 1,781C, 1,782V, 1,783N, 1,803A
CDD
cd00078 (HECTc)

1,538D

catalytic cleft
1,419R, 1,447D, 1,457E, 1,538D, 1,739F, 1,775L, 1,776P, 1,779M, 1,780T, 1,781C, 1,782V, 1,783N, 1,803A
CDD
cd00078 (HECTc)

1,739F

catalytic cleft
1,419R, 1,447D, 1,457E, 1,538D, 1,739F, 1,775L, 1,776P, 1,779M, 1,780T, 1,781C, 1,782V, 1,783N, 1,803A
CDD
cd00078 (HECTc)

1,775L

catalytic cleft
1,419R, 1,447D, 1,457E, 1,538D, 1,739F, 1,775L, 1,776P, 1,779M, 1,780T, 1,781C, 1,782V, 1,783N, 1,803A
CDD
cd00078 (HECTc)

1,776P

catalytic cleft
1,419R, 1,447D, 1,457E, 1,538D, 1,739F, 1,775L, 1,776P, 1,779M, 1,780T, 1,781C, 1,782V, 1,783N, 1,803A
CDD
cd00078 (HECTc)

1,779M

catalytic cleft
1,419R, 1,447D, 1,457E, 1,538D, 1,739F, 1,775L, 1,776P, 1,779M, 1,780T, 1,781C, 1,782V, 1,783N, 1,803A
CDD
cd00078 (HECTc)

1,780T

catalytic cleft
1,419R, 1,447D, 1,457E, 1,538D, 1,739F, 1,775L, 1,776P, 1,779M, 1,780T, 1,781C, 1,782V, 1,783N, 1,803A
CDD
cd00078 (HECTc)

1,781C

catalytic cleft
1,419R, 1,447D, 1,457E, 1,538D, 1,739F, 1,775L, 1,776P, 1,779M, 1,780T, 1,781C, 1,782V, 1,783N, 1,803A
CDD
cd00078 (HECTc)

1,782V

catalytic cleft
1,419R, 1,447D, 1,457E, 1,538D, 1,739F, 1,775L, 1,776P, 1,779M, 1,780T, 1,781C, 1,782V, 1,783N, 1,803A
CDD
cd00078 (HECTc)

1,783N

catalytic cleft
1,419R, 1,447D, 1,457E, 1,538D, 1,739F, 1,775L, 1,776P, 1,779M, 1,780T, 1,781C, 1,782V, 1,783N, 1,803A
CDD
cd00078 (HECTc)

1,803A

catalytic cleft
1,419R, 1,447D, 1,457E, 1,538D, 1,739F, 1,775L, 1,776P, 1,779M, 1,780T, 1,781C, 1,782V, 1,783N, 1,803A
CDD
cd00078 (HECTc)
E2 interaction sit...

1,573T

E2 interaction site
1,573T, 1,576S, 1,577L, 1,579M, 1,580L, 1,583Y, 1,608A, 1,613L, 1,614A, 1,616D, 1,622R, 1,627L, 1,644Y, 1,648V
CDD
cd00078 (HECTc)

1,576S

E2 interaction site
1,573T, 1,576S, 1,577L, 1,579M, 1,580L, 1,583Y, 1,608A, 1,613L, 1,614A, 1,616D, 1,622R, 1,627L, 1,644Y, 1,648V
CDD
cd00078 (HECTc)

1,577L

E2 interaction site
1,573T, 1,576S, 1,577L, 1,579M, 1,580L, 1,583Y, 1,608A, 1,613L, 1,614A, 1,616D, 1,622R, 1,627L, 1,644Y, 1,648V
CDD
cd00078 (HECTc)

1,579M

E2 interaction site
1,573T, 1,576S, 1,577L, 1,579M, 1,580L, 1,583Y, 1,608A, 1,613L, 1,614A, 1,616D, 1,622R, 1,627L, 1,644Y, 1,648V
CDD
cd00078 (HECTc)

1,580L

E2 interaction site
1,573T, 1,576S, 1,577L, 1,579M, 1,580L, 1,583Y, 1,608A, 1,613L, 1,614A, 1,616D, 1,622R, 1,627L, 1,644Y, 1,648V
CDD
cd00078 (HECTc)

1,583Y

E2 interaction site
1,573T, 1,576S, 1,577L, 1,579M, 1,580L, 1,583Y, 1,608A, 1,613L, 1,614A, 1,616D, 1,622R, 1,627L, 1,644Y, 1,648V
CDD
cd00078 (HECTc)

1,608A

E2 interaction site
1,573T, 1,576S, 1,577L, 1,579M, 1,580L, 1,583Y, 1,608A, 1,613L, 1,614A, 1,616D, 1,622R, 1,627L, 1,644Y, 1,648V
CDD
cd00078 (HECTc)

1,613L

E2 interaction site
1,573T, 1,576S, 1,577L, 1,579M, 1,580L, 1,583Y, 1,608A, 1,613L, 1,614A, 1,616D, 1,622R, 1,627L, 1,644Y, 1,648V
CDD
cd00078 (HECTc)

1,614A

E2 interaction site
1,573T, 1,576S, 1,577L, 1,579M, 1,580L, 1,583Y, 1,608A, 1,613L, 1,614A, 1,616D, 1,622R, 1,627L, 1,644Y, 1,648V
CDD
cd00078 (HECTc)

1,616D

E2 interaction site
1,573T, 1,576S, 1,577L, 1,579M, 1,580L, 1,583Y, 1,608A, 1,613L, 1,614A, 1,616D, 1,622R, 1,627L, 1,644Y, 1,648V
CDD
cd00078 (HECTc)

1,622R

E2 interaction site
1,573T, 1,576S, 1,577L, 1,579M, 1,580L, 1,583Y, 1,608A, 1,613L, 1,614A, 1,616D, 1,622R, 1,627L, 1,644Y, 1,648V
CDD
cd00078 (HECTc)

1,627L

E2 interaction site
1,573T, 1,576S, 1,577L, 1,579M, 1,580L, 1,583Y, 1,608A, 1,613L, 1,614A, 1,616D, 1,622R, 1,627L, 1,644Y, 1,648V
CDD
cd00078 (HECTc)

1,644Y

E2 interaction site
1,573T, 1,576S, 1,577L, 1,579M, 1,580L, 1,583Y, 1,608A, 1,613L, 1,614A, 1,616D, 1,622R, 1,627L, 1,644Y, 1,648V
CDD
cd00078 (HECTc)

1,648V

E2 interaction site
1,573T, 1,576S, 1,577L, 1,579M, 1,580L, 1,583Y, 1,608A, 1,613L, 1,614A, 1,616D, 1,622R, 1,627L, 1,644Y, 1,648V
CDD
cd00078 (HECTc)

Protein sequence

>MIA_00555_1
MKNNVVDSSEPTAESSSSDKPVPATRQDSDVIMTGVDDTSDQASQTSSISSNFVNIAGPSSHHTAHEDASIINRPLFSIV
KNSNNNSNASSSTNTPRNIPSDLDGDVALDDDDIYLSSASLQTGLPLEPHPTRADPDIAIEQEPSLLNPDDEDESDDRDL
EDANELVQAINQTNAGFGERLSGDHDDDIDHDLYVNDDDDEDDDEDDDEEDDDEDEHSEAEDDDDDYDEDDDDDDDEDDD
DDDEDDDEEEDNDEENAIRSANEQILQSLRGAFGIPDVVSSIVDRIDHCIEAIKDREDPSNVMIGLQELADVLLMTNEDV
LNDYLPTTKVLDALVDIMVDELYENSVEIIIMTCRCLLNLLDANPMSLNRVSYARPVKALCEKLFEIQYIDTAELALTAL
RKISRSQTHAILENGGLTACLSYLDFFSSSTQRIATETVANTLSSVPLRYFDSVKDAVFLIENILSNNDPEISKNIADSI
CGIIESFNSNSEYINQLMTPKISMKLLSYIDPSVNSVLTLKPRIIKAIATLIRVSTKFTVLAIENKIGSHLYQVLTKHAP
YFDDDETEPINGAYEKNTTAIIQALIYADKEILSNTLQVFSNVYPQVSITAEQEIISPYQYSFSSEIEKERMEKRINDLL
SVSNIVRFSKITLSLLIDTFSSTVDIKIRRSVLQTIIKIVSSLPSGILQQVVANVELSLLLSSILSQRDHDSLTLGALEI
ATILMQKLPDIYIVSFFRGGIIGEIENLIKAEEIKQKNKKDLIEKEKEELAKKLENNNSTNSSSSTTNEEGINSQKDKGN
KEENDGENDEDEMEEEDNNNDEEEREAREEAEAEEDEDEDEESGSDESLGSLVNVVSFYSDLSPLVLSQAKKLVEVYNEK
IKSRNFEDEMKKGLNKLTYLSEKFLAHEDLHANFQEFAKVITNVSEFELLCSHILRSLLTILTTGDNVQTTRKLFLETFL
GGNSTKPFEILLERLNESLSRFERFEVLAADSYSHMRNSISSSLSRQLRISLTPLIKKKETDSEDTSSSFKRAKPIMISI
QATASLKAIDDFYKTKVQAENTIFGSSMKIFSQILALTEDKDDEVKKEEEEAKEEEDEKKKEQEEPKQDDTTENSDKDTV
MEDSEPQKEENSNSDEDEDSSKNNTLQGPSTTAARSVATSSTSRSDALSSLSNGTNPAPWHLEFYIDGVPISHDATVFGA
IFKNLLAKEMEKKEKGEPYTDNPSWKIWGKPHNIWFKKKKGGFSDGKEKPIEAEDLDPILKMPASFGSNESVSIVIRLLN
TLFKMNNSVLDLFTNSGRSTNACTVSSYPKNHISPLSMAKFSNSKLTAKLNRQLEEPLIVSSGMMPSWAVDACRLYPFLF
PFETRLLFLQSTSFGYSRLMNRLQQSQDSSESRGSGGPFGSDGQQHVSSLLRQKIRISRSHMLQSAMKVMTLYGSSPYSL
EVEFFDDVGTGLGPTLEFYATVSKQFTRRKLSIWRDDDGSSVKDSGKNSYVFSKQGLFPLPRDPTWLKTSSSGKKIISTF
KSLGTFVARALLDSRIIDLNFNSLFFRLAAMTEEQLLSAADYIGTVYLVDQHTGKSLDMLQAYVNERKRLTENNASEQDI
KDIRVDGASVEDLALDFRLPGRNNLELIPNGADMEVTIFNIDEYIKRVLDMTLGSGIVEQIKAFKAGFSVVFPYSALSTF
VPDELVVMCGQGEADWSYETLCATAKADHGYNMDSRVVKDLFEVMSKFTVEQRKAFLQFVTGSPNLPIGGFKALRPAFTI
VCRESEPPLTPDEYLPTVMTCVNYFKLPNYSSKEILRERIMTAIKEGLGSFLLS

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0004842 ubiquitin-protein transferase activity
GO:0005488 binding

Cellular Component

None predicted.

Filter view on

Entry type

Status

Per-residue features

MIA_00555_1

Protein function

Protein alignments

Mitochondrial localization by mitoprotII

Protein family membership

Domains and repeats

Detailed signature matches

Residue annotation

Protein sequence

GO term prediction

Biological Process

Molecular Function

Cellular Component