MCA_01892_1

Protein

Length

1,728 amino acids

Gene name: UFD4

Description: Ubiquitin fusion degradation protein 4

Browser: contigA:5790282-5795540+

RNA-seq: read pairs 1779, FPKM 12.7, percentile rank 30.4% (100% = highest expression)

Protein function

Annotation:		UFD4	Ubiquitin fusion degradation protein 4
KEGG:	K10590	TRIP12	E3 ubiquitin-protein ligase TRIP12 [EC:2.3.2.26]
EGGNOG:	0PGIG	UFD4	Ubiquitin-protein ligase
SGD closest match:	S000001493	UFD4	Ubiquitin fusion degradation protein 4
CGD closest match:	CAL0000196972	CAALFM_CR01170WA	Putative ubiquitin-protein ligase

Protein alignments

	%id	Aln length	E-value
MIA_00555_1	56.54%	635	0.0	MIA_00555_1
A0A0J9XEP0_GEOCN	57.93%	599	0.0	Similar to saccharomyces cerevisiae YKL010C UFD4 Ubiquitin-protein ligase (E3) that interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome OS=Geotrichum candidum GN=BN980_GECA12s03145g PE=4 SV=1
UniRef50_A0A0J9XEP0	57.93%	599	0.0	Similar to saccharomyces cerevisiae YKL010C UFD4 Ubiquitin-protein ligase (E3) that interacts with Rpt4p and Rpt6p, two subunits of the 19S particle of the 26S proteasome n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9XEP0_GEOCN
A0A060T950_BLAAD	41.04%	926	0.0	ARAD1C35288p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C35288g PE=4 SV=1
A0A167FFR1_9ASCO	50.87%	633	0.0	Putative ubiquitin-protein ligase UFD4 OS=Sugiyamaella lignohabitans GN=UFD4 PE=4 SV=1
A0A1E3PJY7_9ASCO	45.10%	612	3e-163	Uncharacterized protein (Fragment) OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_5354 PE=4 SV=1
Q6C6X9_YARLI	44.73%	617	1e-154	YALI0E05401p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E05401g PE=4 SV=1
Q5A892_CANAL	41.09%	640	8e-148	Putative ubiquitin-protein ligase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=CAALFM_CR01170WA PE=4 SV=1
A0A1E4THX7_9ASCO	42.35%	614	4e-152	Uncharacterized protein (Fragment) OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_18258 PE=4 SV=1
UFD4_YEAST	39.45%	621	2e-124	Ubiquitin fusion degradation protein 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=UFD4 PE=1 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0177

Protein family membership

None predicted.

Domains and repeats

Domain
10 - 730
Armadillo-type fold (IPR016024)
Armadillo-like helical (IPR011989)
797 - 971
Armadillo-type fold (IPR016024)
1,338 - 1,728
HECT domain (IPR000569)

1 200 400 600 800 1000 1200 1400 1600 1728

Detailed signature matches

IPR016024

Armadillo-type fold
1. SSF48371 (ARM repeat)
  
  797 - 971
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
  
  185 - 205
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
  
  615 - 724
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
  
  263 - 533
  
  SUPERFAMILY
  SSF48371 (ARM repeat)
IPR011989

Armadillo-like helical
1. G3DSA:1.25.10.10
  
  10 - 730
  
  GENE3D
  G3DSA:1.25.10.10 (G3DSA:1.25.10.10)
IPR000569

HECT domain
1. PF00632 (HECT)
  
  1,394 - 1,725
  
  Pfam
  PF00632 (HECT)
2. cd00078 (HECTc)
  
  1,345 - 1,723
  
  CDD
  cd00078 (HECTc)
3. SSF56204 (Hect, E3 ...)
  
  1,338 - 1,721
  
  SUPERFAMILY
  SSF56204 (Hect, E3 ligase catalytic domain)
4. SM00119 (hect_3)
  
  1,356 - 1,728
  
  SMART
  SM00119 (hect_3)
5. PS50237 (HECT)
  
  1,385 - 1,728
  
  PROSITE profiles
  PS50237 (HECT)

no IPR

Unintegrated signatures

Coil

717 - 762

COILS
Coil (Coil)
G3DSA:3.30.21...

1,497 - 1,568

GENE3D
G3DSA:3.30.2160.10 (G3DSA:3.30.2160.10)
G3DSA:3.30.24...

1,604 - 1,728

GENE3D
G3DSA:3.30.2410.10 (G3DSA:3.30.2410.10)
G3DSA:3.90.17...

1,322 - 1,496

GENE3D
G3DSA:3.90.1750.10 (G3DSA:3.90.1750.10)
mobidb-lite (disord...)

1,063 - 1,116

MobiDB Lite
mobidb-lite (disorder_prediction)

1 - 233

MobiDB Lite
mobidb-lite (disorder_prediction)

726 - 838

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

catalytic cleft cd...

1,351R

catalytic cleft
1,351R, 1,379D, 1,389E, 1,464D, 1,653F, 1,689L, 1,690P, 1,693M, 1,694T, 1,695C, 1,696V, 1,697N, 1,717A
CDD
cd00078 (HECTc)

1,379D

catalytic cleft
1,351R, 1,379D, 1,389E, 1,464D, 1,653F, 1,689L, 1,690P, 1,693M, 1,694T, 1,695C, 1,696V, 1,697N, 1,717A
CDD
cd00078 (HECTc)

1,389E

catalytic cleft
1,351R, 1,379D, 1,389E, 1,464D, 1,653F, 1,689L, 1,690P, 1,693M, 1,694T, 1,695C, 1,696V, 1,697N, 1,717A
CDD
cd00078 (HECTc)

1,464D

catalytic cleft
1,351R, 1,379D, 1,389E, 1,464D, 1,653F, 1,689L, 1,690P, 1,693M, 1,694T, 1,695C, 1,696V, 1,697N, 1,717A
CDD
cd00078 (HECTc)

1,653F

catalytic cleft
1,351R, 1,379D, 1,389E, 1,464D, 1,653F, 1,689L, 1,690P, 1,693M, 1,694T, 1,695C, 1,696V, 1,697N, 1,717A
CDD
cd00078 (HECTc)

1,689L

catalytic cleft
1,351R, 1,379D, 1,389E, 1,464D, 1,653F, 1,689L, 1,690P, 1,693M, 1,694T, 1,695C, 1,696V, 1,697N, 1,717A
CDD
cd00078 (HECTc)

1,690P

catalytic cleft
1,351R, 1,379D, 1,389E, 1,464D, 1,653F, 1,689L, 1,690P, 1,693M, 1,694T, 1,695C, 1,696V, 1,697N, 1,717A
CDD
cd00078 (HECTc)

1,693M

catalytic cleft
1,351R, 1,379D, 1,389E, 1,464D, 1,653F, 1,689L, 1,690P, 1,693M, 1,694T, 1,695C, 1,696V, 1,697N, 1,717A
CDD
cd00078 (HECTc)

1,694T

catalytic cleft
1,351R, 1,379D, 1,389E, 1,464D, 1,653F, 1,689L, 1,690P, 1,693M, 1,694T, 1,695C, 1,696V, 1,697N, 1,717A
CDD
cd00078 (HECTc)

1,695C

catalytic cleft
1,351R, 1,379D, 1,389E, 1,464D, 1,653F, 1,689L, 1,690P, 1,693M, 1,694T, 1,695C, 1,696V, 1,697N, 1,717A
CDD
cd00078 (HECTc)

1,696V

catalytic cleft
1,351R, 1,379D, 1,389E, 1,464D, 1,653F, 1,689L, 1,690P, 1,693M, 1,694T, 1,695C, 1,696V, 1,697N, 1,717A
CDD
cd00078 (HECTc)

1,697N

catalytic cleft
1,351R, 1,379D, 1,389E, 1,464D, 1,653F, 1,689L, 1,690P, 1,693M, 1,694T, 1,695C, 1,696V, 1,697N, 1,717A
CDD
cd00078 (HECTc)

1,717A

catalytic cleft
1,351R, 1,379D, 1,389E, 1,464D, 1,653F, 1,689L, 1,690P, 1,693M, 1,694T, 1,695C, 1,696V, 1,697N, 1,717A
CDD
cd00078 (HECTc)
E2 interaction sit...

1,494L

E2 interaction site
1,494L, 1,497S, 1,498M, 1,500F, 1,501L, 1,520D, 1,527L, 1,529L, 1,536Q, 1,541L, 1,558Y, 1,562V
CDD
cd00078 (HECTc)

1,497S

E2 interaction site
1,494L, 1,497S, 1,498M, 1,500F, 1,501L, 1,520D, 1,527L, 1,529L, 1,536Q, 1,541L, 1,558Y, 1,562V
CDD
cd00078 (HECTc)

1,498M

E2 interaction site
1,494L, 1,497S, 1,498M, 1,500F, 1,501L, 1,520D, 1,527L, 1,529L, 1,536Q, 1,541L, 1,558Y, 1,562V
CDD
cd00078 (HECTc)

1,500F

E2 interaction site
1,494L, 1,497S, 1,498M, 1,500F, 1,501L, 1,520D, 1,527L, 1,529L, 1,536Q, 1,541L, 1,558Y, 1,562V
CDD
cd00078 (HECTc)

1,501L

E2 interaction site
1,494L, 1,497S, 1,498M, 1,500F, 1,501L, 1,520D, 1,527L, 1,529L, 1,536Q, 1,541L, 1,558Y, 1,562V
CDD
cd00078 (HECTc)

1,520D

E2 interaction site
1,494L, 1,497S, 1,498M, 1,500F, 1,501L, 1,520D, 1,527L, 1,529L, 1,536Q, 1,541L, 1,558Y, 1,562V
CDD
cd00078 (HECTc)

1,527L

E2 interaction site
1,494L, 1,497S, 1,498M, 1,500F, 1,501L, 1,520D, 1,527L, 1,529L, 1,536Q, 1,541L, 1,558Y, 1,562V
CDD
cd00078 (HECTc)

1,529L

E2 interaction site
1,494L, 1,497S, 1,498M, 1,500F, 1,501L, 1,520D, 1,527L, 1,529L, 1,536Q, 1,541L, 1,558Y, 1,562V
CDD
cd00078 (HECTc)

1,536Q

E2 interaction site
1,494L, 1,497S, 1,498M, 1,500F, 1,501L, 1,520D, 1,527L, 1,529L, 1,536Q, 1,541L, 1,558Y, 1,562V
CDD
cd00078 (HECTc)

1,541L

E2 interaction site
1,494L, 1,497S, 1,498M, 1,500F, 1,501L, 1,520D, 1,527L, 1,529L, 1,536Q, 1,541L, 1,558Y, 1,562V
CDD
cd00078 (HECTc)

1,558Y

E2 interaction site
1,494L, 1,497S, 1,498M, 1,500F, 1,501L, 1,520D, 1,527L, 1,529L, 1,536Q, 1,541L, 1,558Y, 1,562V
CDD
cd00078 (HECTc)

1,562V

E2 interaction site
1,494L, 1,497S, 1,498M, 1,500F, 1,501L, 1,520D, 1,527L, 1,529L, 1,536Q, 1,541L, 1,558Y, 1,562V
CDD
cd00078 (HECTc)

Protein sequence

>MCA_01892_1
MKNFSQDHSEPGSSQNSSNERNEAHIDNTNINDDNDVLMTGTDEDSKSSKSSNANTNDPSNSKTYSKYNLLSILNEPSPS
SPSYSSYNSLREQCDSQTHMGSGSSSSNQANEDNDDDLYATPSSSSRRSENANYADVGSSSRNRNVEPSDEEMFDTIDNP
HEMSDTEYYPSNSRSYDDDDGDDDDDDDDDEDDNDDDDDEEEDDDEDEDDDDNDEENVVENMDEEDDETQDFRSDRDLLF
RTLRGAFGFPESALSSFGMSDQLSSSIEAITQQDDPSTVLTGLQTLAEVLLMSNEDFLNQQLQVQKVLDALVSIMSNPLY
EDNIQMIIMACRCLSNLLDANPSSLDRLSYSKVVKALCEKLFEIQYIDVAELALTALRKISRCQTNAILENGGLTACLSY
LDFFSLSTQRIATEIVANTLRSIPTKYFDSVKDAIPLIENLLGNTDKEIMKNISTAICGIVENFKFSAEKIEALITPTVS
KKLFSYLDPNIATEAMITPTVIKAVGVIVKVSSKLTKTAIESHLISNLYQILTKHAPTFDENDNEVFLPEKNNSTIIQSL
IYTPKDILLNSLTVILNIFPSVGADSEEQKANPYRVNYSSESEETNAKERGEYLLKLSSISTISAISMSLLIDVFSSTVD
IDIRRMVLQSLIKITHFLPPDVLYKSVQNVELSSLLSSIISQEDHPTLVLGSLDIACNLISKLPSFYAVSFFRGGIISEI
ETLLEKEKEKERKKEEEEKAKKALEEDKSQQIAEESEEAEKTTTESGIETPTLLTRVNSSSTSASSNRNLERSNTDDDED
EELDDDDDEDDEEEEEEEDDDDDDYDENEEMERRNISISSQFQTPSFYTDLSPLIVQEATCILSIYESQLETENFSSEIK
EKFNQLIALEEKIRNRDNLAIQFNQLFELIPGVSQFELLRSHILRSLLTVLTTGTESDILNAQKTFMQCAIKDNFQSFET
LINHLNDSLSRFERFEVLSSETSVVNSTPESIRRQFARQVKINLVPLTPKPPKKQKSIVISTQAIASLKAIDDFYKQKVQ
TESDHFSASTGTFTQILDSTVPENTTATSSSSNLPTSGNSSTTSVNSTSNENTNNAGPSNSGSSHASDTESIEQSRKNVK
IEDISVRNSHSNDFNWHLDFYLDGKPIPTETTIYGAIYKSLAAKEEEKKLKGEHYRTDITQLVWSRAHVINFKKKPGSWV
PFKDNSLEAESIDPSVEVPKSFGSNASLSIVVRLLNTLFRMNSNLTDFFDPNTIKTVNPVALTSFSSSKLTAKLNRQLEE
PLIVVSGIVPQWTVDACRLYPFLFPFETRHLFLQSTSFGHSRLMNKWQNEINERNRRSMDRIPRQKVRISRDHMFYNGIK
LMELYGSSPYALEVEFFDDVGTGLGPTLEFYTTFSKDFTQKSLNMWRNDDGDSESKYVFSKNGLFPKPHDEKYFSTKQGK
HLLSLFRSLGIFVARSLIDSRIIDLNFNPLFFRISQFGASHQNIGMLSLVDKQLENSMKFLERFVEARDSQGSSGEVKVD
NSSIDDLSLDFTLPGQSDIELVPGGSNINVNIDNVDEYIKLVLDMTLGAGIAKQIASFKEGISTIMPYSALHSFTPEELV
VLCGQEEADWTYETLCTSIKADHGYTLDSPVVQYLLEVMAEFTKEERKAFLQFSTGSRNLPIGGFPSLRPPFTVVCRDSD
PPYTPNDYLPTVMTCVNYFKLPNYSSKEILRERLLTAMNEGSGCFLLS

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0004842 ubiquitin-protein transferase activity
GO:0005488 binding

Cellular Component

None predicted.

Filter view on

Entry type

Status

Per-residue features

MCA_01892_1

Protein function

Protein alignments

Mitochondrial localization by mitoprotII

Protein family membership

Domains and repeats

Detailed signature matches

Residue annotation

Protein sequence

GO term prediction

Biological Process

Molecular Function

Cellular Component