MIA_00541_1

Protein

Length

476 amino acids

Protein function

EGGNOG:	0PIDE	TUB2	Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain
SGD closest match:	S000001857	TUB2	Tubulin beta chain
CGD closest match:	CAL0000176403	TUB2	Tubulin beta chain

Protein alignments

	%id	Aln length	E-value
MCA_04907_1	59.674%	429	0.0	MCA_04907_1
TBB_YEAST	60.230%	435	5.10e-180	Tubulin beta chain OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TUB2 PE=1 SV=2
A0A0J9XKX6_GEOCN	60.373%	429	3.42e-179	Tubulin beta chain OS=Geotrichum candidum GN=BN980_GECA32s02265g PE=3 SV=1
A0A1E3PFH7_9ASCO	57.931%	435	9.73e-178	Tubulin beta chain OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_47457 PE=3 SV=1
A0A060T354_BLAAD	57.110%	436	1.08e-176	Tubulin beta chain OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1A06952g PE=3 SV=1
UniRef50_Q8SS99	57.373%	434	8.54e-171	Tubulin beta chain n=119 Tax=Eukaryota TaxID=2759 RepID=TBB_ENCCU
Q6C7H8_YARLI	57.011%	435	2.90e-173	Tubulin beta chain OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E00726g PE=3 SV=1
A0A1D8PC97_CANAL	57.343%	429	2.27e-172	Tubulin beta chain OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=TUB2 PE=3 SV=1
A0A1E4TEH0_9ASCO	57.798%	436	3.58e-170	Tubulin beta chain (Fragment) OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_60471 PE=3 SV=1
A0A161HF60_9ASCO	57.471%	348	2.13e-135	Tubulin beta chain OS=Sugiyamaella lignohabitans GN=TUB2 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0121

Protein family membership

Tubulin (IPR000217)
- Beta tubulin (IPR002453)

Domains and repeats

Domain
1 - 265
Tubulin/FtsZ, GTPase domain (IPR003008)
Domain
246 - 430
Tubulin/FtsZ, C-terminal (IPR008280)
Domain
249 - 385
Tubulin/FtsZ, 2-layer sandwich domain (IPR018316)

1 50 100 150 200 250 300 350 400 450 476

Detailed signature matches

IPR000217

Tubulin
1. PR01161 (TUBULIN)
  
  108 - 132
  
  PRINTS
  PR01161 (TUBULIN)
  
  372 - 400
  
  PRINTS
  PR01161 (TUBULIN)
  
  95 - 106
  
  PRINTS
  PR01161 (TUBULIN)
  
  10 - 30
  
  PRINTS
  PR01161 (TUBULIN)
  
  134 - 152
  
  PRINTS
  PR01161 (TUBULIN)
  
  153 - 174
  
  PRINTS
  PR01161 (TUBULIN)
  
  178 - 191
  
  PRINTS
  PR01161 (TUBULIN)
  
  192 - 212
  
  PRINTS
  PR01161 (TUBULIN)
  
  53 - 72
  
  PRINTS
  PR01161 (TUBULIN)
IPR002453

Beta tubulin
1. PR01163 (BETATUBULIN)
  
  379 - 390
  
  PRINTS
  PR01163 (BETATUBULIN)
  
  110 - 128
  
  PRINTS
  PR01163 (BETATUBULIN)
  
  349 - 372
  
  PRINTS
  PR01163 (BETATUBULIN)
  
  232 - 241
  
  PRINTS
  PR01163 (BETATUBULIN)
  
  90 - 101
  
  PRINTS
  PR01163 (BETATUBULIN)
  
  414 - 432
  
  PRINTS
  PR01163 (BETATUBULIN)
  
  42 - 59
  
  PRINTS
  PR01163 (BETATUBULIN)
  
  214 - 226
  
  PRINTS
  PR01163 (BETATUBULIN)
  
  154 - 166
  
  PRINTS
  PR01163 (BETATUBULIN)
  
  331 - 345
  
  PRINTS
  PR01163 (BETATUBULIN)
  
  247 - 260
  
  PRINTS
  PR01163 (BETATUBULIN)
  
  267 - 283
  
  PRINTS
  PR01163 (BETATUBULIN)
  
  313 - 331
  
  PRINTS
  PR01163 (BETATUBULIN)
IPR003008

Tubulin/FtsZ, GTPase domain
1. PF00091 (Tubulin)
  
  3 - 214
  
  Pfam
  PF00091 (Tubulin)
2. SM00864 (Tubulin_4)
  
  48 - 246
  
  SMART
  SM00864 (Tubulin_4)
3. G3DSA:3.40.50...
  
  1 - 265
  
  GENE3D
  G3DSA:3.40.50.1440 (G3DSA:3.40.50.1440)
4. SSF52490 (Tubulin n...)
  
  1 - 245
  
  SUPERFAMILY
  SSF52490 (Tubulin nucleotide-binding domain-like)
IPR008280

Tubulin/FtsZ, C-terminal
1. SSF55307 (Tubulin C...)
  
  246 - 430
  
  SUPERFAMILY
  SSF55307 (Tubulin C-terminal domain-like)
IPR018316

Tubulin/FtsZ, 2-layer sandwich domain
1. PF03953 (Tubulin_C)
  
  263 - 384
  
  Pfam
  PF03953 (Tubulin_C)
2. SM00865 (Tubulin_C_4)
  
  249 - 385
  
  SMART
  SM00865 (Tubulin_C_4)
3. G3DSA:3.30.13...
  
  266 - 374
  
  GENE3D
  G3DSA:3.30.1330.20 (G3DSA:3.30.1330.20)
IPR013838

Beta tubulin, autoregulation binding site
1. PS00228 (TUBULIN_B_...)
  
  1 - 4
  
  PROSITE patterns
  PS00228 (TUBULIN_B_AUTOREG)
IPR017975

Tubulin, conserved site
1. PS00227 (TUBULIN)
  
  142 - 148
  
  PROSITE patterns
  PS00227 (TUBULIN)

no IPR

Unintegrated signatures

Coil

410 - 430

COILS
Coil (Coil)
G3DSA:1.10.28...

375 - 431

GENE3D
G3DSA:1.10.287.600 (G3DSA:1.10.287.600)
cd02187 (beta_tubulin)

2 - 428

CDD
cd02187 (beta_tubulin)

Residue annotation

beta/alpha domain ...

2R

beta/alpha domain interface
2R, 133T, 247H, 248H, 249N, 253R, 258N, 260V, 261P, 262F, 324S, 325I, 326R, 329E, 349D, 350N, 351T, 352Q, 353T
CDD
cd02187 (beta_tubulin)

133T

beta/alpha domain interface
2R, 133T, 247H, 248H, 249N, 253R, 258N, 260V, 261P, 262F, 324S, 325I, 326R, 329E, 349D, 350N, 351T, 352Q, 353T
CDD
cd02187 (beta_tubulin)

247H

beta/alpha domain interface
2R, 133T, 247H, 248H, 249N, 253R, 258N, 260V, 261P, 262F, 324S, 325I, 326R, 329E, 349D, 350N, 351T, 352Q, 353T
CDD
cd02187 (beta_tubulin)

248H

beta/alpha domain interface
2R, 133T, 247H, 248H, 249N, 253R, 258N, 260V, 261P, 262F, 324S, 325I, 326R, 329E, 349D, 350N, 351T, 352Q, 353T
CDD
cd02187 (beta_tubulin)

249N

beta/alpha domain interface
2R, 133T, 247H, 248H, 249N, 253R, 258N, 260V, 261P, 262F, 324S, 325I, 326R, 329E, 349D, 350N, 351T, 352Q, 353T
CDD
cd02187 (beta_tubulin)

253R

beta/alpha domain interface
2R, 133T, 247H, 248H, 249N, 253R, 258N, 260V, 261P, 262F, 324S, 325I, 326R, 329E, 349D, 350N, 351T, 352Q, 353T
CDD
cd02187 (beta_tubulin)

258N

beta/alpha domain interface
2R, 133T, 247H, 248H, 249N, 253R, 258N, 260V, 261P, 262F, 324S, 325I, 326R, 329E, 349D, 350N, 351T, 352Q, 353T
CDD
cd02187 (beta_tubulin)

260V

beta/alpha domain interface
2R, 133T, 247H, 248H, 249N, 253R, 258N, 260V, 261P, 262F, 324S, 325I, 326R, 329E, 349D, 350N, 351T, 352Q, 353T
CDD
cd02187 (beta_tubulin)

261P

beta/alpha domain interface
2R, 133T, 247H, 248H, 249N, 253R, 258N, 260V, 261P, 262F, 324S, 325I, 326R, 329E, 349D, 350N, 351T, 352Q, 353T
CDD
cd02187 (beta_tubulin)

262F

beta/alpha domain interface
2R, 133T, 247H, 248H, 249N, 253R, 258N, 260V, 261P, 262F, 324S, 325I, 326R, 329E, 349D, 350N, 351T, 352Q, 353T
CDD
cd02187 (beta_tubulin)

324S

beta/alpha domain interface
2R, 133T, 247H, 248H, 249N, 253R, 258N, 260V, 261P, 262F, 324S, 325I, 326R, 329E, 349D, 350N, 351T, 352Q, 353T
CDD
cd02187 (beta_tubulin)

325I

beta/alpha domain interface
2R, 133T, 247H, 248H, 249N, 253R, 258N, 260V, 261P, 262F, 324S, 325I, 326R, 329E, 349D, 350N, 351T, 352Q, 353T
CDD
cd02187 (beta_tubulin)

326R

beta/alpha domain interface
2R, 133T, 247H, 248H, 249N, 253R, 258N, 260V, 261P, 262F, 324S, 325I, 326R, 329E, 349D, 350N, 351T, 352Q, 353T
CDD
cd02187 (beta_tubulin)

329E

beta/alpha domain interface
2R, 133T, 247H, 248H, 249N, 253R, 258N, 260V, 261P, 262F, 324S, 325I, 326R, 329E, 349D, 350N, 351T, 352Q, 353T
CDD
cd02187 (beta_tubulin)

349D

beta/alpha domain interface
2R, 133T, 247H, 248H, 249N, 253R, 258N, 260V, 261P, 262F, 324S, 325I, 326R, 329E, 349D, 350N, 351T, 352Q, 353T
CDD
cd02187 (beta_tubulin)

350N

beta/alpha domain interface
2R, 133T, 247H, 248H, 249N, 253R, 258N, 260V, 261P, 262F, 324S, 325I, 326R, 329E, 349D, 350N, 351T, 352Q, 353T
CDD
cd02187 (beta_tubulin)

351T

beta/alpha domain interface
2R, 133T, 247H, 248H, 249N, 253R, 258N, 260V, 261P, 262F, 324S, 325I, 326R, 329E, 349D, 350N, 351T, 352Q, 353T
CDD
cd02187 (beta_tubulin)

352Q

beta/alpha domain interface
2R, 133T, 247H, 248H, 249N, 253R, 258N, 260V, 261P, 262F, 324S, 325I, 326R, 329E, 349D, 350N, 351T, 352Q, 353T
CDD
cd02187 (beta_tubulin)

353T

beta/alpha domain interface
2R, 133T, 247H, 248H, 249N, 253R, 258N, 260V, 261P, 262F, 324S, 325I, 326R, 329E, 349D, 350N, 351T, 352Q, 353T
CDD
cd02187 (beta_tubulin)
nucleotide binding...

10G

nucleotide binding site
10G, 11Q, 12C, 15Q, 140S, 142G, 143G, 144G, 145T, 146G, 183E, 206N, 224H, 228N
CDD
cd02187 (beta_tubulin)

11Q

nucleotide binding site
10G, 11Q, 12C, 15Q, 140S, 142G, 143G, 144G, 145T, 146G, 183E, 206N, 224H, 228N
CDD
cd02187 (beta_tubulin)

12C

nucleotide binding site
10G, 11Q, 12C, 15Q, 140S, 142G, 143G, 144G, 145T, 146G, 183E, 206N, 224H, 228N
CDD
cd02187 (beta_tubulin)

15Q

nucleotide binding site
10G, 11Q, 12C, 15Q, 140S, 142G, 143G, 144G, 145T, 146G, 183E, 206N, 224H, 228N
CDD
cd02187 (beta_tubulin)

140S

nucleotide binding site
10G, 11Q, 12C, 15Q, 140S, 142G, 143G, 144G, 145T, 146G, 183E, 206N, 224H, 228N
CDD
cd02187 (beta_tubulin)

142G

nucleotide binding site
10G, 11Q, 12C, 15Q, 140S, 142G, 143G, 144G, 145T, 146G, 183E, 206N, 224H, 228N
CDD
cd02187 (beta_tubulin)

143G

nucleotide binding site
10G, 11Q, 12C, 15Q, 140S, 142G, 143G, 144G, 145T, 146G, 183E, 206N, 224H, 228N
CDD
cd02187 (beta_tubulin)

144G

nucleotide binding site
10G, 11Q, 12C, 15Q, 140S, 142G, 143G, 144G, 145T, 146G, 183E, 206N, 224H, 228N
CDD
cd02187 (beta_tubulin)

145T

nucleotide binding site
10G, 11Q, 12C, 15Q, 140S, 142G, 143G, 144G, 145T, 146G, 183E, 206N, 224H, 228N
CDD
cd02187 (beta_tubulin)

146G

nucleotide binding site
10G, 11Q, 12C, 15Q, 140S, 142G, 143G, 144G, 145T, 146G, 183E, 206N, 224H, 228N
CDD
cd02187 (beta_tubulin)

183E

nucleotide binding site
10G, 11Q, 12C, 15Q, 140S, 142G, 143G, 144G, 145T, 146G, 183E, 206N, 224H, 228N
CDD
cd02187 (beta_tubulin)

206N

nucleotide binding site
10G, 11Q, 12C, 15Q, 140S, 142G, 143G, 144G, 145T, 146G, 183E, 206N, 224H, 228N
CDD
cd02187 (beta_tubulin)

224H

nucleotide binding site
10G, 11Q, 12C, 15Q, 140S, 142G, 143G, 144G, 145T, 146G, 183E, 206N, 224H, 228N
CDD
cd02187 (beta_tubulin)

228N

nucleotide binding site
10G, 11Q, 12C, 15Q, 140S, 142G, 143G, 144G, 145T, 146G, 183E, 206N, 224H, 228N
CDD
cd02187 (beta_tubulin)
Taxol binding site...

22E

Taxol binding site
22E, 23T, 26A, 27E, 217L, 226D, 229T, 230I, 236S, 272F, 274S, 275L, 276V, 360S, 361Q, 362T
CDD
cd02187 (beta_tubulin)

23T

Taxol binding site
22E, 23T, 26A, 27E, 217L, 226D, 229T, 230I, 236S, 272F, 274S, 275L, 276V, 360S, 361Q, 362T
CDD
cd02187 (beta_tubulin)

26A

Taxol binding site
22E, 23T, 26A, 27E, 217L, 226D, 229T, 230I, 236S, 272F, 274S, 275L, 276V, 360S, 361Q, 362T
CDD
cd02187 (beta_tubulin)

27E

Taxol binding site
22E, 23T, 26A, 27E, 217L, 226D, 229T, 230I, 236S, 272F, 274S, 275L, 276V, 360S, 361Q, 362T
CDD
cd02187 (beta_tubulin)

217L

Taxol binding site
22E, 23T, 26A, 27E, 217L, 226D, 229T, 230I, 236S, 272F, 274S, 275L, 276V, 360S, 361Q, 362T
CDD
cd02187 (beta_tubulin)

226D

Taxol binding site
22E, 23T, 26A, 27E, 217L, 226D, 229T, 230I, 236S, 272F, 274S, 275L, 276V, 360S, 361Q, 362T
CDD
cd02187 (beta_tubulin)

229T

Taxol binding site
22E, 23T, 26A, 27E, 217L, 226D, 229T, 230I, 236S, 272F, 274S, 275L, 276V, 360S, 361Q, 362T
CDD
cd02187 (beta_tubulin)

230I

Taxol binding site
22E, 23T, 26A, 27E, 217L, 226D, 229T, 230I, 236S, 272F, 274S, 275L, 276V, 360S, 361Q, 362T
CDD
cd02187 (beta_tubulin)

236S

Taxol binding site
22E, 23T, 26A, 27E, 217L, 226D, 229T, 230I, 236S, 272F, 274S, 275L, 276V, 360S, 361Q, 362T
CDD
cd02187 (beta_tubulin)

272F

Taxol binding site
22E, 23T, 26A, 27E, 217L, 226D, 229T, 230I, 236S, 272F, 274S, 275L, 276V, 360S, 361Q, 362T
CDD
cd02187 (beta_tubulin)

274S

Taxol binding site
22E, 23T, 26A, 27E, 217L, 226D, 229T, 230I, 236S, 272F, 274S, 275L, 276V, 360S, 361Q, 362T
CDD
cd02187 (beta_tubulin)

275L

Taxol binding site
22E, 23T, 26A, 27E, 217L, 226D, 229T, 230I, 236S, 272F, 274S, 275L, 276V, 360S, 361Q, 362T
CDD
cd02187 (beta_tubulin)

276V

Taxol binding site
22E, 23T, 26A, 27E, 217L, 226D, 229T, 230I, 236S, 272F, 274S, 275L, 276V, 360S, 361Q, 362T
CDD
cd02187 (beta_tubulin)

360S

Taxol binding site
22E, 23T, 26A, 27E, 217L, 226D, 229T, 230I, 236S, 272F, 274S, 275L, 276V, 360S, 361Q, 362T
CDD
cd02187 (beta_tubulin)

361Q

Taxol binding site
22E, 23T, 26A, 27E, 217L, 226D, 229T, 230I, 236S, 272F, 274S, 275L, 276V, 360S, 361Q, 362T
CDD
cd02187 (beta_tubulin)

362T

Taxol binding site
22E, 23T, 26A, 27E, 217L, 226D, 229T, 230I, 236S, 272F, 274S, 275L, 276V, 360S, 361Q, 362T
CDD
cd02187 (beta_tubulin)
alpha/beta domain ...

72P

alpha/beta domain interface
72P, 73G, 100G, 101N, 102N, 105K, 181V, 183E, 224H
CDD
cd02187 (beta_tubulin)

73G

alpha/beta domain interface
72P, 73G, 100G, 101N, 102N, 105K, 181V, 183E, 224H
CDD
cd02187 (beta_tubulin)

100G

alpha/beta domain interface
72P, 73G, 100G, 101N, 102N, 105K, 181V, 183E, 224H
CDD
cd02187 (beta_tubulin)

101N

alpha/beta domain interface
72P, 73G, 100G, 101N, 102N, 105K, 181V, 183E, 224H
CDD
cd02187 (beta_tubulin)

102N

alpha/beta domain interface
72P, 73G, 100G, 101N, 102N, 105K, 181V, 183E, 224H
CDD
cd02187 (beta_tubulin)

105K

alpha/beta domain interface
72P, 73G, 100G, 101N, 102N, 105K, 181V, 183E, 224H
CDD
cd02187 (beta_tubulin)

181V

alpha/beta domain interface
72P, 73G, 100G, 101N, 102N, 105K, 181V, 183E, 224H
CDD
cd02187 (beta_tubulin)

183E

alpha/beta domain interface
72P, 73G, 100G, 101N, 102N, 105K, 181V, 183E, 224H
CDD
cd02187 (beta_tubulin)

224H

alpha/beta domain interface
72P, 73G, 100G, 101N, 102N, 105K, 181V, 183E, 224H
CDD
cd02187 (beta_tubulin)

Protein sequence

>MIA_00541_1
MREIIHLSVGQCGNQVGNAFWETICAEHGISTDGSYTGSTPDSQLKRTNVYFNELSRHKRYVPRSIIVDLEPGPIDALRS
GPLGLLFKPDNYIFSQASAGNNWAKGFYTEGADLAEEIMDAIRREVEQCDLLTGFQLSHSLGGGTGSGLGALIVSKLKEE
YPDRMMATFSILPSPRVSNTVVEPYNATLATNQLIEHASEVFCFDNEAIFDICKKTLLSSQPSHKDLNTIIAQAASGITT
SLRFPGHHNTDLRKLAVNLVPFPRLHFLTVAFASLVSASAASFRTMSAPELINQLFAPSSVLIAADPQRGKYLTIAASFR
GKLSIRDIEEHMKIARNKNRSQFVQWIPDNTQTSLCTVPSQTHNVSATFIANNTCIAEVFSRIHAQFTPMFRRKAFLHNY
THEGMEEAEFSEAEQNIIDLLNQYKQYQDTDPQDDLNMISSANCNEGLEFNDESTFYSFNERAPHRDHNSTTINVG

GO term prediction

Biological Process

GO:0007017 microtubule-based process

Molecular Function

GO:0003924 GTPase activity
GO:0005200 structural constituent of cytoskeleton
GO:0005525 GTP binding

Cellular Component

GO:0005874 microtubule

Filter view on

Entry type

Status

Per-residue features