MIA_00294_1

Protein

Length

2,253 amino acids

Protein function

EGGNOG:	0PFDZ	FG09638.1	Bifunctional pyrimidine biosynthesis protein (PyrABCN)
SGD closest match:	S000003666	URA2	Protein URA2
CGD closest match:	CAL0000196578	URA2	Bifunctional carbamoylphosphate synthetase/aspartate transcarbamylase

Protein alignments

	%id	Aln length
MCA_01241_1	83.333%	2268	MCA_01241_1
A0A0J9X615_GEOCN	77.704%	2247	Similar to Saccharomyces cerevisiae YJL130C URA2 Bifunctional carbamoylphosphate synthetase (CPSase)-aspartate transcarbamylase (ATCase) OS=Geotrichum candidum GN=BN980_GECA04s01220g PE=3 SV=1
A0A167ETX0_9ASCO	74.074%	2241	Bifunctional carbamoylphosphate synthetase/aspartate transcarbamylase OS=Sugiyamaella lignohabitans GN=URA2 PE=3 SV=1
A0A1E3PEK1_9ASCO	73.003%	2241	Carbamoyl-phosphate synth OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_83947 PE=3 SV=1
A0A060T6W0_BLAAD	71.733%	2250	ARAD1B15246p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1B15246g PE=3 SV=1
Q6C6H5_YARLI	71.825%	2236	YALI0E09493p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E09493g PE=3 SV=1
A0A1E4TB48_9ASCO	69.720%	2249	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_32390 PE=3 SV=1
UniRef50_Q75BK8	68.930%	2253	ACR263Cp n=46 Tax=Fungi TaxID=4751 RepID=Q75BK8_ASHGO
A0A1D8PTD1_CANAL	69.118%	2257	Bifunctional carbamoylphosphate synthetase/aspartate transcarbamylase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=URA2 PE=3 SV=1
PYR1_YEAST	68.115%	2255	Protein URA2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=URA2 PE=1 SV=5

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.1429

Protein family membership

Carbamoyl-phosphate synthase, small subunit (IPR006274)

Carbamoyl-phosphate synthase, large subunit (IPR006275)

Aspartate/ornithine carbamoyltransferase (IPR006130)
- Aspartate carbamoyltransferase (IPR002082)

Domains and repeats

Domain
13 - 168
Carbamoyl-phosphate synthase small subunit, N-terminal domain (IPR002474)
184 - 401
Class I glutamine amidotransferase-like (IPR029062)
Glutamine amidotransferase (IPR017926)
Carbamoyl-phosphate synthase small subunit, GATase1 domain (IPR035686)
809 - 963
Carbamoyl-phosphate synthetase, large subunit oligomerisation domain (IPR005480)
1,064 - 1,153
ATP-grasp fold, subdomain 1 (IPR013815)
1,335 - 1,490
Methylglyoxal synthase-like domain (IPR011607)
1,496 - 1,769
Metal-dependent hydrolase (IPR032466)
1,948 - 2,089
Aspartate/ornithine carbamoyltransferase, carbamoyl-P binding (IPR006132)
2,096 - 2,245
Aspartate/ornithine carbamoyltransferase, Asp/Orn-binding domain (IPR006131)
Domain
424 - 547
Pre-ATP-grasp domain (IPR016185)
965 - 1,083
Pre-ATP-grasp domain (IPR016185)
Domain
438 - 452
Carbamoyl-phosphate synthase large subunit, CPSase domain (IPR005483)
467 - 477
Carbamoyl-phosphate synthase large subunit, CPSase domain (IPR005483)
545 - 748
ATP-grasp fold (IPR011761)
Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domain (IPR005479)
Carbamoyl-phosphate synthase large subunit, CPSase domain (IPR005483)
793 - 811
Carbamoyl-phosphate synthase large subunit, CPSase domain (IPR005483)
1,083 - 1,281
ATP-grasp fold (IPR011761)
Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domain (IPR005479)

1 500 1000 1500 2000 2253

Detailed signature matches

IPR006274

Carbamoyl-phosphate synthase, small subunit
1. MF_01209 (CPSase_S_...)
  
  13 - 399
  
  HAMAP
  MF_01209 (CPSase_S_chain)
2. TIGR01368
  
  17 - 395
  
  TIGRFAMs
  TIGR01368 (TIGR01368)
IPR006275

Carbamoyl-phosphate synthase, large subunit
1. TIGR01369
  
  423 - 1,472
  
  TIGRFAMs
  TIGR01369 (TIGR01369)
IPR006130

Aspartate/ornithine carbamoyltransferase
1. PR00100 (AOTCASE)
  
  2,202 - 2,211
  
  PRINTS
  PR00100 (AOTCASE)
  
  2,212 - 2,235
  
  PRINTS
  PR00100 (AOTCASE)
  
  1,993 - 2,012
  
  PRINTS
  PR00100 (AOTCASE)
  
  2,076 - 2,087
  
  PRINTS
  PR00100 (AOTCASE)
2. G3DSA:3.40.50...
  
  1,935 - 2,088
  
  GENE3D
  G3DSA:3.40.50.1370 (G3DSA:3.40.50.1370)
  
  2,089 - 2,250
  
  GENE3D
  G3DSA:3.40.50.1370 (G3DSA:3.40.50.1370)
3. PS00097 (CARBAMOYLT...)
  
  1,993 - 2,000
  
  PROSITE patterns
  PS00097 (CARBAMOYLTRANSFERASE)
4. SSF53671 (Aspartate...)
  
  1,945 - 2,248
  
  SUPERFAMILY
  SSF53671 (Aspartate/ornithine carbamoyltransferase)
IPR002082

Aspartate carbamoyltransferase
1. TIGR00670
  
  1,948 - 2,247
  
  TIGRFAMs
  TIGR00670 (TIGR00670)
2. PR00101 (ATCASE)
  
  2,021 - 2,030
  
  PRINTS
  PR00101 (ATCASE)
  
  2,075 - 2,092
  
  PRINTS
  PR00101 (ATCASE)
  
  2,206 - 2,211
  
  PRINTS
  PR00101 (ATCASE)
  
  2,167 - 2,176
  
  PRINTS
  PR00101 (ATCASE)
  
  1,983 - 2,005
  
  PRINTS
  PR00101 (ATCASE)
  
  2,228 - 2,242
  
  PRINTS
  PR00101 (ATCASE)
3. MF_00001 (Asp_carb_tr)
  
  1,947 - 2,249
  
  HAMAP
  MF_00001 (Asp_carb_tr)
IPR002474

Carbamoyl-phosphate synthase small subunit, N-terminal domain
1. SSF52021 (Carbamoyl...)
  
  17 - 161
  
  SUPERFAMILY
  SSF52021 (Carbamoyl phosphate synthetase, small subunit N-terminal domain)
2. G3DSA:3.50.30.20
  
  13 - 168
  
  GENE3D
  G3DSA:3.50.30.20 (G3DSA:3.50.30.20)
3. SM01097 (CPSase_sm_...)
  
  19 - 155
  
  SMART
  SM01097 (CPSase_sm_chain_2)
4. PF00988 (CPSase_sm_...)
  
  18 - 154
  
  Pfam
  PF00988 (CPSase_sm_chain)
IPR029062

Class I glutamine amidotransferase-like
1. G3DSA:3.40.50...
  
  184 - 400
  
  GENE3D
  G3DSA:3.40.50.880 (G3DSA:3.40.50.880)
2. SSF52317 (Class I g...)
  
  210 - 398
  
  SUPERFAMILY
  SSF52317 (Class I glutamine amidotransferase-like)
IPR017926

Glutamine amidotransferase
1. PF00117 (GATase)
  
  216 - 393
  
  Pfam
  PF00117 (GATase)
2. PS51273 (GATASE_TYPE_1)
  
  213 - 401
  
  PROSITE profiles
  PS51273 (GATASE_TYPE_1)
IPR035686

Carbamoyl-phosphate synthase small subunit, GATase1 domain
1. cd01744 (GATase1_CP...)
  
  214 - 392
  
  CDD
  cd01744 (GATase1_CPSase)
IPR016185

Pre-ATP-grasp domain
1. SSF52440 (PreATP-gr...)
  
  965 - 1,083
  
  SUPERFAMILY
  SSF52440 (PreATP-grasp domain)
  
  424 - 547
  
  SUPERFAMILY
  SSF52440 (PreATP-grasp domain)
IPR011761

ATP-grasp fold
1. PS50975 (ATP_GRASP)
  
  1,087 - 1,278
  
  PROSITE profiles
  PS50975 (ATP_GRASP)
  
  550 - 742
  
  PROSITE profiles
  PS50975 (ATP_GRASP)
IPR005479

Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domain
1. PF02786 (CPSase_L_D2)
  
  545 - 748
  
  Pfam
  PF02786 (CPSase_L_D2)
  
  1,083 - 1,281
  
  Pfam
  PF02786 (CPSase_L_D2)
2. PS00867 (CPSASE_2)
  
  711 - 718
  
  PROSITE patterns
  PS00867 (CPSASE_2)
  
  1,247 - 1,254
  
  PROSITE patterns
  PS00867 (CPSASE_2)
3. PS00866 (CPSASE_1)
  
  1,118 - 1,132
  
  PROSITE patterns
  PS00866 (CPSASE_1)
  
  581 - 595
  
  PROSITE patterns
  PS00866 (CPSASE_1)
IPR005483

Carbamoyl-phosphate synthase large subunit, CPSase domain
1. PR00098 (CPSASE)
  
  654 - 671
  
  PRINTS
  PR00098 (CPSASE)
  
  619 - 638
  
  PRINTS
  PR00098 (CPSASE)
  
  793 - 811
  
  PRINTS
  PR00098 (CPSASE)
  
  711 - 740
  
  PRINTS
  PR00098 (CPSASE)
  
  438 - 452
  
  PRINTS
  PR00098 (CPSASE)
  
  467 - 477
  
  PRINTS
  PR00098 (CPSASE)
  
  585 - 597
  
  PRINTS
  PR00098 (CPSASE)
IPR005480

Carbamoyl-phosphate synthetase, large subunit oligomerisation domain
1. SSF48108 (Carbamoyl...)
  
  809 - 963
  
  SUPERFAMILY
  SSF48108 (Carbamoyl phosphate synthetase, large subunit connection domain)
2. PF02787 (CPSase_L_D3)
  
  836 - 956
  
  Pfam
  PF02787 (CPSase_L_D3)
3. SM01096 (CPSase_L_D3_2)
  
  833 - 956
  
  SMART
  SM01096 (CPSase_L_D3_2)
4. G3DSA:1.10.10...
  
  815 - 947
  
  GENE3D
  G3DSA:1.10.1030.10 (G3DSA:1.10.1030.10)
IPR013815

ATP-grasp fold, subdomain 1
1. G3DSA:3.30.14...
  
  1,064 - 1,153
  
  GENE3D
  G3DSA:3.30.1490.20 (G3DSA:3.30.1490.20)
IPR011607

Methylglyoxal synthase-like domain
1. PF02142 (MGS)
  
  1,363 - 1,460
  
  Pfam
  PF02142 (MGS)
2. G3DSA:3.40.50...
  
  1,335 - 1,473
  
  GENE3D
  G3DSA:3.40.50.1380 (G3DSA:3.40.50.1380)
3. SSF52335 (Methylgly...)
  
  1,348 - 1,490
  
  SUPERFAMILY
  SSF52335 (Methylglyoxal synthase-like)
4. SM00851 (MGS_2a)
  
  1,363 - 1,460
  
  SMART
  SM00851 (MGS_2a)
IPR032466

Metal-dependent hydrolase
1. SSF51556 (Metallo-d...)
  
  1,496 - 1,769
  
  SUPERFAMILY
  SSF51556 (Metallo-dependent hydrolases)
IPR006132

Aspartate/ornithine carbamoyltransferase, carbamoyl-P binding
1. PF02729 (OTCace_N)
  
  1,948 - 2,089
  
  Pfam
  PF02729 (OTCace_N)
IPR006131

Aspartate/ornithine carbamoyltransferase, Asp/Orn-binding domain
1. PF00185 (OTCace)
  
  2,096 - 2,245
  
  Pfam
  PF00185 (OTCace)

no IPR

Unintegrated signatures

G3DSA:3.20.20...

1,507 - 1,604

GENE3D
G3DSA:3.20.20.140 (G3DSA:3.20.20.140)

1,605 - 1,799

GENE3D
G3DSA:3.20.20.140 (G3DSA:3.20.20.140)
G3DSA:3.30.47...

1,154 - 1,334

GENE3D
G3DSA:3.30.470.20 (G3DSA:3.30.470.20)

537 - 814

GENE3D
G3DSA:3.30.470.20 (G3DSA:3.30.470.20)
G3DSA:3.40.50.20

948 - 1,063

GENE3D
G3DSA:3.40.50.20 (G3DSA:3.40.50.20)

423 - 534

GENE3D
G3DSA:3.40.50.20 (G3DSA:3.40.50.20)
PR00099 (CPSGATASE)

327 - 338

PRINTS
PR00099 (CPSGATASE)

302 - 319

PRINTS
PR00099 (CPSGATASE)

214 - 228

PRINTS
PR00099 (CPSGATASE)

250 - 264

PRINTS
PR00099 (CPSGATASE)

285 - 301

PRINTS
PR00099 (CPSGATASE)
SSF56059 (Glutathio...)

545 - 814

SUPERFAMILY
SSF56059 (Glutathione synthetase ATP-binding domain-like)

1,082 - 1,341

SUPERFAMILY
SSF56059 (Glutathione synthetase ATP-binding domain-like)
cd01423 (MGS_CPS_I_III)

1,349 - 1,470

CDD
cd01423 (MGS_CPS_I_III)

Residue annotation

catalytic site cd0...

290C

catalytic site
290C, 374H, 376E
CDD
cd01744 (GATase1_CPSase)

374H

catalytic site
290C, 374H, 376E
CDD
cd01744 (GATase1_CPSase)

376E

catalytic site
290C, 374H, 376E
CDD
cd01744 (GATase1_CPSase)
subunit interface ...

315N

subunit interface
315N, 317P, 324G, 326C, 353A, 379P, 380G, 382R, 383D
CDD
cd01744 (GATase1_CPSase)

317P

subunit interface
315N, 317P, 324G, 326C, 353A, 379P, 380G, 382R, 383D
CDD
cd01744 (GATase1_CPSase)

324G

subunit interface
315N, 317P, 324G, 326C, 353A, 379P, 380G, 382R, 383D
CDD
cd01744 (GATase1_CPSase)

326C

subunit interface
315N, 317P, 324G, 326C, 353A, 379P, 380G, 382R, 383D
CDD
cd01744 (GATase1_CPSase)

353A

subunit interface
315N, 317P, 324G, 326C, 353A, 379P, 380G, 382R, 383D
CDD
cd01744 (GATase1_CPSase)

379P

subunit interface
315N, 317P, 324G, 326C, 353A, 379P, 380G, 382R, 383D
CDD
cd01744 (GATase1_CPSase)

380G

subunit interface
315N, 317P, 324G, 326C, 353A, 379P, 380G, 382R, 383D
CDD
cd01744 (GATase1_CPSase)

382R

subunit interface
315N, 317P, 324G, 326C, 353A, 379P, 380G, 382R, 383D
CDD
cd01744 (GATase1_CPSase)

383D

subunit interface
315N, 317P, 324G, 326C, 353A, 379P, 380G, 382R, 383D
CDD
cd01744 (GATase1_CPSase)
probable substrate...

1,354S

probable substrate binding site
1,354S, 1,361K, 1,381T, 1,383G, 1,384T, 1,427N, 1,428L, 1,430S
CDD
cd01423 (MGS_CPS_I_III)

1,361K

probable substrate binding site
1,354S, 1,361K, 1,381T, 1,383G, 1,384T, 1,427N, 1,428L, 1,430S
CDD
cd01423 (MGS_CPS_I_III)

1,381T

probable substrate binding site
1,354S, 1,361K, 1,381T, 1,383G, 1,384T, 1,427N, 1,428L, 1,430S
CDD
cd01423 (MGS_CPS_I_III)

1,383G

probable substrate binding site
1,354S, 1,361K, 1,381T, 1,383G, 1,384T, 1,427N, 1,428L, 1,430S
CDD
cd01423 (MGS_CPS_I_III)

1,384T

probable substrate binding site
1,354S, 1,361K, 1,381T, 1,383G, 1,384T, 1,427N, 1,428L, 1,430S
CDD
cd01423 (MGS_CPS_I_III)

1,427N

probable substrate binding site
1,354S, 1,361K, 1,381T, 1,383G, 1,384T, 1,427N, 1,428L, 1,430S
CDD
cd01423 (MGS_CPS_I_III)

1,428L

probable substrate binding site
1,354S, 1,361K, 1,381T, 1,383G, 1,384T, 1,427N, 1,428L, 1,430S
CDD
cd01423 (MGS_CPS_I_III)

1,430S

probable substrate binding site
1,354S, 1,361K, 1,381T, 1,383G, 1,384T, 1,427N, 1,428L, 1,430S
CDD
cd01423 (MGS_CPS_I_III)

Protein sequence

>MIA_00294_1
MLFQNSLSSLPSNLFKFSLKDGTLIQGYSFGADKSAAGELVFQTGMVGYPESITDPSYQGQILVITFPLVGNYGVPSRTE
IDPIVKSLPKYFESSKIHVAGLIVASYTEEYSHYLATSSLGTWLKEEGIPAIYAVDTRSLTKYIRTRGSTLCKILSSKQN
TPSPQFPADFNTIEWSDPNTRNLVSEVSHKQPTLYAPPTDRPLLKGPDGKVIRILAIDVGMKYNQIRCFVNRGVELKVVP
WDYDFSSEEYDGLFISNGPGNPQTVAQVSERLQPLLDQNSKSLKPIFGICLGHQLLAIASGASTHKLKFGNRGHNIPATN
LISGRCYITSQNHGYAVDVETLTSGWKELFVNANDGSNEGIYHESLPFFSVQFHPESTPGPRDTEFLFDTFLQTVVDFKT
TKTLKPVTFPGGTRAENDLKHPRVNVQKVLVLGSGGLSIGQAGEFDYSGSQAIKALKEEGIYTILINPNIATIQTSKGLA
DKVYFLPVTPEFVRKVIKYEHPDAIYVTFGGQTALNVGIQLKDEFESLGVKVLGTPIDTIITTEDRELFARSMEEIGEKV
AKSQTASSIPEALDAVKGIGFPVIVRAAYALGGLGSGFAENEAQLIELCNKAFAASPQVLVERSMKGWKEIEYEVVRDAF
DNCITVCNMENFDPLGIHTGDSIVVAPSQTLSDEDYNMLRTTAVNVIRHLGVVGECNIQYALNPFSKEYCIIEVNARLSR
SSALASKATGYPLAFIAAKLGLNIRLDEIKNKVTKVTCSCFEPSLDYVVVKIPRWDLKKFTRVSKELSSSMKSVGEVMSI
GRTFEEAIQKAIRSTDYHNLGFNSTDALMSIDIDTELQTPSDQRLFAIANALDSGYSVDKVWSMTKIDKWFLNKLNGLVQ
FAKSLSNYKVHQIPLNLIREAKQLGFEDRQIAKFVGSNEITVRRRRVEAGITPFVKQIDTVAAEFPAFTNYLYLTYNADS
HDIEFNDHGFMVLGSGVYRIGSSVEFDWCAVRTVRTLREQGAKTIMINYNPETVSTDYDEADRLYFEVINLERILDIYDL
EASSGVVVSMGGQTSNNIALTLHRQNVKILGTSPEMIDSAENRYKFSRMLDTIGVDQPAWKELTSFEEAETFADKVGYPV
LVRPSYVLSGAAMNTVYSKDDLKSYLQQAVEVSRDYPVVITKYIENAKEIEMDAVAKDGKLLMHVVSEHVENAGVHSGDA
TLIVPPQDLDPETVRRIVVATAKIGNALNVTGPFNIQFIAKDNEIKVIECNVRASRSFPFVSKVLGVDLIEMATKAIAGY
DVSPYPPVEIPEDYVAIKVPQFSFSRLSGADPILGVEMASTGEVATFGHNKYEAYLKSLIATGFTLPKKNILLSVGSYKE
KMELLPSVKKLHQLGYSLFATSGTADFIQEHGIPVQYLEDLDEAEGEKSEYSLTQHLANHLIDLYINLPSNNRFRRPASY
VSKGYRTRRMAVDYAIPLVTNVKNAKLLIEAIARNSSLEISSVDSQSSHRTVSLPGLISISTFIPGLTDASTGAEDASKV
LKTALASGFTMNRIMPISSDGLLTDATELKIAQDNVKNNAATDYNFSVAATDSNFGELSQITGTVGSLVVPYHYISNNSN
TVTTVSEHFTHWPSYKPIVTDAKAASIASILLLASLHNRPIHITNVTNKQDINLIGLSKEKGLRVTCDVSIYSLFFAAED
FPEVKSNLLPSKADIAALWENLKVIDIFSIGGLPSRLALELGSKDVSGAGIADALPLLFSAVNDGRLTIDDIVLRLYTNP
KRIFELHDQPETVVEIDLDRVSEALKHSQTFSPLANKPLRGFVERVTFNGRVVALGGKLTSTEFTGRDYSSVLLPHVAAL
AAGLNTVSGVSALSSGNSSSITESSGAPLAPIITTPGSPRAKRVSFSTADLKSPLYSRAELASSVIKEDDSVIDTPSVEA
AEQFVAIREPTPPSAIREALRNSPFLRRHVLSVQQFSRSDLHRLFATAQEMRLAVERVGVLDVLRGRVLTTLFFEPSTRT
SCSFNAAMQRLGGRVVAVATESSSAKKGETLQDTIRTMACYSDAIVLRHPEESSAAIAGKYSPIPVINGGNGSVEHPTQA
FLDLFTIREELGTVNGINVTFVGDLKYGRPVHSLCKLLQHYNVKVTLVSPNELSLPESLKANLRSTGHLATESELLTDSI
IASSDVIYCTRVQKERFANVADFERLKDTYIIDNAVLAKAKKHMCILHPLPRNNEISEEVDFDNRAAYFRQMRYGLFVRM
ALLAMVIGATDSA

GO term prediction

Biological Process

GO:0006207 'de novo' pyrimidine nucleobase biosynthetic process
GO:0006520 cellular amino acid metabolic process
GO:0006541 glutamine metabolic process
GO:0006807 nitrogen compound metabolic process

Molecular Function

GO:0003824 catalytic activity
GO:0004070 aspartate carbamoyltransferase activity
GO:0004088 carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity
GO:0005524 ATP binding
GO:0016597 amino acid binding
GO:0016743 carboxyl- or carbamoyltransferase activity
GO:0046872 metal ion binding

Cellular Component

None predicted.

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Entry type

Status

Per-residue features