MIA_00264_1

Protein

Length

354 amino acids

Protein function

EGGNOG:	0PHRR	FG10857.1	asparaginase
CGD closest match:	CAL0000188326	FGR10	Fgr10p

Protein alignments

	%id	Aln length	E-value
MCA_01031_1	42.899%	345	9.27e-78	MCA_01031_1
A0A0J9XKG0_GEOCN	41.840%	337	2.97e-49	Uncharacterized protein OS=Geotrichum candidum GN=BN980_GECA23s00362g PE=4 SV=1
UniRef50_A0A0J9XKG0	41.840%	337	6.07e-46	Uncharacterized protein n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9XKG0_GEOCN
A0A060T446_BLAAD	30.747%	348	6.75e-28	ARAD1C38764p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C38764g PE=4 SV=1
A0A1E4TIV9_9ASCO	30.573%	314	3.51e-19	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_252 PE=4 SV=1
Q6BZU9_YARLI	31.310%	313	7.61e-18	YALI0F30723p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_F30723g PE=4 SV=1
A0A167F5M9_9ASCO	33.908%	174	1.24e-16	L-asparaginase (Predicted) OS=Sugiyamaella lignohabitans GN=AWJ20_2472 PE=4 SV=1
A0A1D8PM71_CANAL	24.688%	320	4.22e-08	Fgr10p OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=FGR10 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0845

Protein family membership

Peptidase T2, asparaginase 2 (IPR000246)

Domains and repeats

Domain
7 - 328
Nucleophile aminohydrolases, N-terminal (IPR029055)

1 50 100 150 200 250 300 354

Detailed signature matches

IPR000246

Peptidase T2, asparaginase 2
1. PF01112 (Asparagina...)
  
  10 - 265
  
  Pfam
  PF01112 (Asparaginase_2)
IPR029055

Nucleophile aminohydrolases, N-terminal
1. SSF56235 (N-termina...)
  
  7 - 328
  
  SUPERFAMILY
  SSF56235 (N-terminal nucleophile aminohydrolases (Ntn hydrolases))

no IPR

Unintegrated signatures

Coil

39 - 59

COILS
Coil (Coil)
G3DSA:3.60.20.30

181 - 341

GENE3D
G3DSA:3.60.20.30 (G3DSA:3.60.20.30)
cd04514 (Taspase1_like)

9 - 342

CDD
cd04514 (Taspase1_like)

Residue annotation

active site cd04514

14V

active site
14V, 15G, 65T, 66N, 181T, 182V
CDD
cd04514 (Taspase1_like)

15G

active site
14V, 15G, 65T, 66N, 181T, 182V
CDD
cd04514 (Taspase1_like)

65T

active site
14V, 15G, 65T, 66N, 181T, 182V
CDD
cd04514 (Taspase1_like)

66N

active site
14V, 15G, 65T, 66N, 181T, 182V
CDD
cd04514 (Taspase1_like)

181T

active site
14V, 15G, 65T, 66N, 181T, 182V
CDD
cd04514 (Taspase1_like)

182V

active site
14V, 15G, 65T, 66N, 181T, 182V
CDD
cd04514 (Taspase1_like)
dimer interface cd...

75E

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

86M

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

90G

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

91K

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

92A

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

97A

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

127R

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

128V

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

129R

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

130P

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

131A

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

132L

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

133L

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

134V

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

137G

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

141Y

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

202V

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

203A

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

204L

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

205K

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

207A

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

208G

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

209R

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

210V

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

215V

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

216V

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

221Y

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

240E

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

243M

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

244S

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

246Q

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

249R

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)

250V

dimer interface
75E, 86M, 90G, 91K, 92A, 97A, 127R, 128V, 129R, 130P, 131A, 132L, 133L, 134V, 137G, 141Y, 202V, 203A, 204L, 205K, 207A, 208G, 209R, 210V, 215V, 216V, 221Y, 240E, 243M, 244S, 246Q, 249R, 250V
CDD
cd04514 (Taspase1_like)
catalytic nucleoph...

181T

catalytic nucleophile
181T
CDD
cd04514 (Taspase1_like)

Protein sequence

>MIA_00264_1
MQNHQPVAFIALHVGAGNHGPASEKKLKKLCKLVCSSGLEQLKAEAEALDVAVKSCAQLEDSPYTNAGLGAQLNEEGRVE
CDACIMHSNGKAAAVGAVTQAHNAVEVCGEMLRDLMDPTRVKDPFDRVRPALLVGAGANKYAKQHGCLLVANETDLVTDQ
SLREYLQLRQYLQTAEGVQDTVGVICGDSLGNVVTCASSGGVALKVAGRVGPAGVVGAGAYARKGPGKVTAAACTSGTGE
DIMSIQVARVVCDRLCAAGPDGVDATWMEENLIRPEAVETGVFQRGPRLYFGILTAYVSGPGAVEIGFAHTTEAMIIGYA
VAGGRGPVAEVSRNDNLSSGLVYGGYGYKTEDAK

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0016787 hydrolase activity

Cellular Component

None predicted.

Filter view on

Entry type

Status

Per-residue features

MIA_00264_1

Protein function

Protein alignments

Mitochondrial localization by mitoprotII

Protein family membership

Domains and repeats

Detailed signature matches

Residue annotation

Protein sequence

GO term prediction

Biological Process

Molecular Function

Cellular Component