MCA_01031_1

Protein

Length

344 amino acids

RNA-seq: read pairs 247, FPKM 8.8, percentile rank 24.0% (100% = highest expression)

Protein function

KEGG:	K08657	TASP1	taspase, threonine aspartase, 1 [EC:3.4.25.-]
EGGNOG:	0PHRR	FG10857.1	asparaginase
CGD closest match:	CAL0000188326	FGR10	Fgr10p

Protein alignments

	%id	Aln length	E-value
MIA_00264_1	45.77%	343	2e-80	MIA_00264_1
A0A0J9XKG0_GEOCN	37.83%	341	5e-56	Uncharacterized protein OS=Geotrichum candidum GN=BN980_GECA23s00362g PE=4 SV=1
UniRef50_A0A0J9XKG0	37.83%	341	1e-52	Uncharacterized protein n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9XKG0_GEOCN
A0A060T446_BLAAD	34.03%	335	4e-35	ARAD1C38764p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C38764g PE=4 SV=1
Q6BZU9_YARLI	31.19%	311	1e-31	YALI0F30723p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_F30723g PE=4 SV=1
A0A167F5M9_9ASCO	31.08%	370	1e-31	L-asparaginase (Predicted) OS=Sugiyamaella lignohabitans GN=AWJ20_2472 PE=4 SV=1
A0A1E3PF46_9ASCO	29.70%	266	2e-19	N-terminal nucleophile aminohydrolase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_47904 PE=4 SV=1
A0A1D8PM71_CANAL	26.47%	340	2e-16	Fgr10p OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=FGR10 PE=4 SV=1
A0A1E4TIV9_9ASCO	26.28%	312	4e-12	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_252 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0068

Protein family membership

Peptidase T2, asparaginase 2 (IPR000246)

Domains and repeats

Domain
6 - 323
Nucleophile aminohydrolases, N-terminal (IPR029055)

1 50 100 150 200 250 300 344

Detailed signature matches

IPR000246

Peptidase T2, asparaginase 2
1. PF01112 (Asparagina...)
  
  7 - 319
  
  Pfam
  PF01112 (Asparaginase_2)
IPR029055

Nucleophile aminohydrolases, N-terminal
1. SSF56235 (N-termina...)
  
  6 - 323
  
  SUPERFAMILY
  SSF56235 (N-terminal nucleophile aminohydrolases (Ntn hydrolases))

no IPR

Unintegrated signatures

G3DSA:3.60.20.30

182 - 333

GENE3D
G3DSA:3.60.20.30 (G3DSA:3.60.20.30)
cd04514 (Taspase1_like)

7 - 337

CDD
cd04514 (Taspase1_like)

Residue annotation

active site cd04514

13V

active site
13V, 14G, 64T, 65N, 182T, 183V
CDD
cd04514 (Taspase1_like)

14G

active site
13V, 14G, 64T, 65N, 182T, 183V
CDD
cd04514 (Taspase1_like)

64T

active site
13V, 14G, 64T, 65N, 182T, 183V
CDD
cd04514 (Taspase1_like)

65N

active site
13V, 14G, 64T, 65N, 182T, 183V
CDD
cd04514 (Taspase1_like)

182T

active site
13V, 14G, 64T, 65N, 182T, 183V
CDD
cd04514 (Taspase1_like)

183V

active site
13V, 14G, 64T, 65N, 182T, 183V
CDD
cd04514 (Taspase1_like)
dimer interface cd...

74D

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

85I

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

90I

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

91G

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

96A

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

125R

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

126I

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

127K

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

128P

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

129S

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

130F

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

131L

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

132V

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

135G

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

139F

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

203V

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

204A

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

205L

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

206K

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

208P

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

209G

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

210R

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

211V

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

216L

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

217I

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

222E

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

224I

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

240E

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

243M

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

244M

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

246Q

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

249N

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)

250K

dimer interface
74D, 85I, 90I, 91G, 96A, 125R, 126I, 127K, 128P, 129S, 130F, 131L, 132V, 135G, 139F, 203V, 204A, 205L, 206K, 208P, 209G, 210R, 211V, 216L, 217I, 222E, 224I, 240E, 243M, 244M, 246Q, 249N, 250K
CDD
cd04514 (Taspase1_like)
catalytic nucleoph...

182T

catalytic nucleophile
182T
CDD
cd04514 (Taspase1_like)

Protein sequence

>MCA_01031_1
MDNSHIPFIAVHVGAGNHSGKLEKRLAQLCKAVCNEGIEKLALNGGALECAIEACKKLECNPNTNAGFGSPLNDQGKVEC
DSSIIISPGIGASVGAISKAYNPIEVAGNLLKNLINKDKKDPFGRIKPSFLVGEGADDFAKESNCQLIDDDKYDLIAHKS
ITDYIQWKSYIKSASSLPIQDTVGVICGDKTGKIVVCASSGGVALKHPGRVGPAALIGSGIELISQNQVTVASCTSGSGE
DIMMIQAANKICNQLMASGSLDKDWIELHLIEKYAQFTQQSPLYMGALHVISTPGAIEIGYWHTTESMIMAYSTGSKHHA
HCHISKQEKPFQLVMGGWRDISIY

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0016787 hydrolase activity

Cellular Component