MIA_00220_1

Protein

Length

1,065 amino acids

Protein function

EGGNOG:	0PFFK	FG08422.1	IQ and HECT domain protein
SGD closest match:	S000003109	HUL5	Probable E3 ubiquitin-protein ligase HUL5
CGD closest match:	CAL0000193884	orf19.4191.1	Ubiquitin-ubiquitin ligase

Protein alignments

	%id	Aln length	E-value
MCA_01711_1	44.165%	1114	0.0	MCA_01711_1
A0A167E2Z4_9ASCO	37.163%	1114	0.0	Ubiquitin-ubiquitin ligase HUL5 OS=Sugiyamaella lignohabitans GN=HUL5 PE=4 SV=1
UniRef50_A0A167E2Z4	37.163%	1114	0.0	Ubiquitin-ubiquitin ligase HUL5 n=1 Tax=Sugiyamaella lignohabitans TaxID=796027 RepID=A0A167E2Z4_9ASCO
A0A0J9X6J0_GEOCN	40.661%	878	0.0	Similar to Saccharomyces cerevisiae YGL141W HUL5 Multiubiquitin chain assembly factor (E4) OS=Geotrichum candidum GN=BN980_GECA03s01308g PE=4 SV=1
A0A060T6Q4_BLAAD	41.851%	724	1.45e-170	ARAD1B17292p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1B17292g PE=4 SV=1
A0A1E3PD44_9ASCO	48.264%	605	6.67e-175	HECT-domain-containing protein (Fragment) OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_9595 PE=4 SV=1
A0A1D8PL41_CANAL	36.212%	718	1.75e-124	Ubiquitin-ubiquitin ligase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=orf19.4191.1 PE=4 SV=1
Q6CGC9_YARLI	37.887%	710	9.89e-118	YALI0A20372p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_A20372g PE=4 SV=1
HUL5_YEAST	38.699%	584	1.19e-104	Probable E3 ubiquitin-protein ligase HUL5 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HUL5 PE=1 SV=1
A0A1E4T9N4_9ASCO	51.163%	172	4.53e-55	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_29700 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.3016
Predicted cleavage: 23

Protein family membership

None predicted.

Domains and repeats

Domain
684 - 1,065
HECT domain (IPR000569)

1 100 200 300 400 500 600 700 800 900 1000 1065

Detailed signature matches

IPR000569

HECT domain
1. PF00632 (HECT)
  
  783 - 1,065
  
  Pfam
  PF00632 (HECT)
2. cd00078 (HECTc)
  
  691 - 1,063
  
  CDD
  cd00078 (HECTc)
3. SSF56204 (Hect, E3 ...)
  
  684 - 1,057
  
  SUPERFAMILY
  SSF56204 (Hect, E3 ligase catalytic domain)
4. SM00119 (hect_3)
  
  705 - 1,065
  
  SMART
  SM00119 (hect_3)
5. PS50237 (HECT)
  
  707 - 1,065
  
  PROSITE profiles
  PS50237 (HECT)

no IPR

Unintegrated signatures

Coil

1,064 - 1,065

COILS
Coil (Coil)
G3DSA:3.30.24...

952 - 1,065

GENE3D
G3DSA:3.30.2410.10 (G3DSA:3.30.2410.10)
G3DSA:3.90.17...

650 - 866

GENE3D
G3DSA:3.90.1750.10 (G3DSA:3.90.1750.10)
mobidb-lite (disord...)

590 - 629

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

catalytic cleft cd...

693R

catalytic cleft
693R, 722E, 736E, 816D, 996F, 1,027L, 1,028P, 1,031S, 1,032T, 1,033C, 1,034I, 1,035N, 1,055S, 1,062F
CDD
cd00078 (HECTc)

722E

catalytic cleft
693R, 722E, 736E, 816D, 996F, 1,027L, 1,028P, 1,031S, 1,032T, 1,033C, 1,034I, 1,035N, 1,055S, 1,062F
CDD
cd00078 (HECTc)

736E

catalytic cleft
693R, 722E, 736E, 816D, 996F, 1,027L, 1,028P, 1,031S, 1,032T, 1,033C, 1,034I, 1,035N, 1,055S, 1,062F
CDD
cd00078 (HECTc)

816D

catalytic cleft
693R, 722E, 736E, 816D, 996F, 1,027L, 1,028P, 1,031S, 1,032T, 1,033C, 1,034I, 1,035N, 1,055S, 1,062F
CDD
cd00078 (HECTc)

996F

catalytic cleft
693R, 722E, 736E, 816D, 996F, 1,027L, 1,028P, 1,031S, 1,032T, 1,033C, 1,034I, 1,035N, 1,055S, 1,062F
CDD
cd00078 (HECTc)

1,027L

catalytic cleft
693R, 722E, 736E, 816D, 996F, 1,027L, 1,028P, 1,031S, 1,032T, 1,033C, 1,034I, 1,035N, 1,055S, 1,062F
CDD
cd00078 (HECTc)

1,028P

catalytic cleft
693R, 722E, 736E, 816D, 996F, 1,027L, 1,028P, 1,031S, 1,032T, 1,033C, 1,034I, 1,035N, 1,055S, 1,062F
CDD
cd00078 (HECTc)

1,031S

catalytic cleft
693R, 722E, 736E, 816D, 996F, 1,027L, 1,028P, 1,031S, 1,032T, 1,033C, 1,034I, 1,035N, 1,055S, 1,062F
CDD
cd00078 (HECTc)

1,032T

catalytic cleft
693R, 722E, 736E, 816D, 996F, 1,027L, 1,028P, 1,031S, 1,032T, 1,033C, 1,034I, 1,035N, 1,055S, 1,062F
CDD
cd00078 (HECTc)

1,033C

catalytic cleft
693R, 722E, 736E, 816D, 996F, 1,027L, 1,028P, 1,031S, 1,032T, 1,033C, 1,034I, 1,035N, 1,055S, 1,062F
CDD
cd00078 (HECTc)

1,034I

catalytic cleft
693R, 722E, 736E, 816D, 996F, 1,027L, 1,028P, 1,031S, 1,032T, 1,033C, 1,034I, 1,035N, 1,055S, 1,062F
CDD
cd00078 (HECTc)

1,035N

catalytic cleft
693R, 722E, 736E, 816D, 996F, 1,027L, 1,028P, 1,031S, 1,032T, 1,033C, 1,034I, 1,035N, 1,055S, 1,062F
CDD
cd00078 (HECTc)

1,055S

catalytic cleft
693R, 722E, 736E, 816D, 996F, 1,027L, 1,028P, 1,031S, 1,032T, 1,033C, 1,034I, 1,035N, 1,055S, 1,062F
CDD
cd00078 (HECTc)

1,062F

catalytic cleft
693R, 722E, 736E, 816D, 996F, 1,027L, 1,028P, 1,031S, 1,032T, 1,033C, 1,034I, 1,035N, 1,055S, 1,062F
CDD
cd00078 (HECTc)
E2 interaction sit...

848L

E2 interaction site
848L, 851N, 852L, 854K, 855L, 858Y, 865L, 867L, 871I, 872N, 874E, 880K, 885L, 902Y, 906V
CDD
cd00078 (HECTc)

851N

E2 interaction site
848L, 851N, 852L, 854K, 855L, 858Y, 865L, 867L, 871I, 872N, 874E, 880K, 885L, 902Y, 906V
CDD
cd00078 (HECTc)

852L

E2 interaction site
848L, 851N, 852L, 854K, 855L, 858Y, 865L, 867L, 871I, 872N, 874E, 880K, 885L, 902Y, 906V
CDD
cd00078 (HECTc)

854K

E2 interaction site
848L, 851N, 852L, 854K, 855L, 858Y, 865L, 867L, 871I, 872N, 874E, 880K, 885L, 902Y, 906V
CDD
cd00078 (HECTc)

855L

E2 interaction site
848L, 851N, 852L, 854K, 855L, 858Y, 865L, 867L, 871I, 872N, 874E, 880K, 885L, 902Y, 906V
CDD
cd00078 (HECTc)

858Y

E2 interaction site
848L, 851N, 852L, 854K, 855L, 858Y, 865L, 867L, 871I, 872N, 874E, 880K, 885L, 902Y, 906V
CDD
cd00078 (HECTc)

865L

E2 interaction site
848L, 851N, 852L, 854K, 855L, 858Y, 865L, 867L, 871I, 872N, 874E, 880K, 885L, 902Y, 906V
CDD
cd00078 (HECTc)

867L

E2 interaction site
848L, 851N, 852L, 854K, 855L, 858Y, 865L, 867L, 871I, 872N, 874E, 880K, 885L, 902Y, 906V
CDD
cd00078 (HECTc)

871I

E2 interaction site
848L, 851N, 852L, 854K, 855L, 858Y, 865L, 867L, 871I, 872N, 874E, 880K, 885L, 902Y, 906V
CDD
cd00078 (HECTc)

872N

E2 interaction site
848L, 851N, 852L, 854K, 855L, 858Y, 865L, 867L, 871I, 872N, 874E, 880K, 885L, 902Y, 906V
CDD
cd00078 (HECTc)

874E

E2 interaction site
848L, 851N, 852L, 854K, 855L, 858Y, 865L, 867L, 871I, 872N, 874E, 880K, 885L, 902Y, 906V
CDD
cd00078 (HECTc)

880K

E2 interaction site
848L, 851N, 852L, 854K, 855L, 858Y, 865L, 867L, 871I, 872N, 874E, 880K, 885L, 902Y, 906V
CDD
cd00078 (HECTc)

885L

E2 interaction site
848L, 851N, 852L, 854K, 855L, 858Y, 865L, 867L, 871I, 872N, 874E, 880K, 885L, 902Y, 906V
CDD
cd00078 (HECTc)

902Y

E2 interaction site
848L, 851N, 852L, 854K, 855L, 858Y, 865L, 867L, 871I, 872N, 874E, 880K, 885L, 902Y, 906V
CDD
cd00078 (HECTc)

906V

E2 interaction site
848L, 851N, 852L, 854K, 855L, 858Y, 865L, 867L, 871I, 872N, 874E, 880K, 885L, 902Y, 906V
CDD
cd00078 (HECTc)

Protein sequence

>MIA_00220_1
MYGMNFTGQHRRARHINLGGRNATASSKDSVVRRAQFDRERRERARKEETAAIRIQSFYRARKEVESVRREIETQWNSSF
LNPSELVMSPPQLTDCLLRFNFFFKYTCTGSPTNEQQVKAILQILRTNIGQFQQKQIDAHLVVTLIDTLSTVLKPKTKHN
QQTIESAPKPIDADILYALELLQDFSSTGLLPTCGAYIKSITANHITLTASSKYYEQNTEAITLVRDSLIKIISHQIEGH
SHAIDFFASFLSTPGLHVFFTPSQFLPYSSALKSFITKYSALDPSERPSITQQDMVWRLVNFYQFFGSLADQSLFYQTLN
IFISPLNIQIEPPSKTTTAKRLKKLSENPIFMLSTNEFVIEKLNQLTSRSFFTSIMDTLLGSSDFNLPLFSSIVTSLTSL
YPIKKQQIVMFITLSPLAVIQQLWETIKSTPLCKTILEKSPEKSVLARILLDEKPLPPDQVNGAQTISLLFLFLDLYSYW
LIVANDAEFHGTSTTHGLPKQNIKDLAQFLRNICFALLWNAAEISADQKLNSLLNVAPLSVTNSCVLVMRQIYIRDSRRQ
FLPNDFWLMINNFQIEHFISSVVQEEEQLRRENEARSMEVDDDVDDSDSDDDDDVEQESYRQSRISDSNPRSLGGIVPRL
EILRQAPFFIPFDTRLKIFQEFIENLRAEEAALNPPTLNFFGLEARQTGVIRRDHLLEDAYTAFNKLGSDFRQQIAVRFS
NEYGVEAGVGAGITKEFLTIISKTAFNPSHDNEYNIETKEGLFVASGEHLLFPNPILGVSRKYTNLDPEEKLDANRYMVF
LGKIIGKCLYENILVDVEFAPFFLQKWAAAGMGYRNSFDDLYSFDPELYENLIKLYNYPGNVEDLMLDFSINQEVGHGQK
SVVDLKPNGSNIPVTNANRLEYIHAVANYKLNIVLHQQTSLFLQGLSVILPPRWLSMFNAREIQMLISGRPAAINIDDLR
KNSVLINFDENDVTIKYFWEVIKELSDEDKSRFIKFVTSVPKAPLLGFEALNPHFAIRNAGPDEQRLPTSSTCINLIKLP
AYKSKKILKEKILNSIHADAGFELS

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0004842 ubiquitin-protein transferase activity

Cellular Component

None predicted.

Filter view on

Entry type

Status

Per-residue features

MIA_00220_1

Protein function

Protein alignments

Mitochondrial localization by mitoprotII

Protein family membership

Domains and repeats

Detailed signature matches

Residue annotation

Protein sequence

GO term prediction

Biological Process

Molecular Function

Cellular Component