MCA_01711_1

Protein

Length

1,071 amino acids

RNA-seq: read pairs 1633, FPKM 18.8, percentile rank 39.8% (100% = highest expression)

Protein function

KEGG:	K10589	UBE3C	ubiquitin-protein ligase E3 C [EC:2.3.2.26]
EGGNOG:	0PFFK	FG08422.1	IQ and HECT domain protein
SGD closest match:	S000003109	HUL5	Probable E3 ubiquitin-protein ligase HUL5
CGD closest match:	CAL0000193884	orf19.4191.1	Ubiquitin-ubiquitin ligase

Protein alignments

	%id	Aln length	E-value
MIA_00220_1	43.99%	1114	0.0	MIA_00220_1
A0A167E2Z4_9ASCO	36.46%	1108	4e-171	Ubiquitin-ubiquitin ligase HUL5 OS=Sugiyamaella lignohabitans GN=HUL5 PE=4 SV=1
UniRef50_A0A167E2Z4	36.46%	1108	1e-167	Ubiquitin-ubiquitin ligase HUL5 n=1 Tax=Sugiyamaella lignohabitans TaxID=796027 RepID=A0A167E2Z4_9ASCO
A0A0J9X6J0_GEOCN	43.00%	721	1e-166	Similar to Saccharomyces cerevisiae YGL141W HUL5 Multiubiquitin chain assembly factor (E4) OS=Geotrichum candidum GN=BN980_GECA03s01308g PE=4 SV=1
A0A060T6Q4_BLAAD	43.93%	708	1e-162	ARAD1B17292p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1B17292g PE=4 SV=1
A0A1E3PD44_9ASCO	43.85%	618	6e-143	HECT-domain-containing protein (Fragment) OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_9595 PE=4 SV=1
Q6CGC9_YARLI	36.07%	671	7e-108	YALI0A20372p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_A20372g PE=4 SV=1
A0A1D8PL41_CANAL	34.19%	737	1e-104	Ubiquitin-ubiquitin ligase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=orf19.4191.1 PE=4 SV=1
HUL5_YEAST	45.66%	438	2e-95	Probable E3 ubiquitin-protein ligase HUL5 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=HUL5 PE=1 SV=1
A0A1E4T9N4_9ASCO	49.42%	172	5e-43	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_29700 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.4071
Predicted cleavage: 26

Protein family membership

None predicted.

Domains and repeats

Domain
693 - 1,071
HECT domain (IPR000569)

1 100 200 300 400 500 600 700 800 900 1000 1071

Detailed signature matches

IPR000569

HECT domain
1. PF00632 (HECT)
  
  764 - 1,071
  
  Pfam
  PF00632 (HECT)
2. cd00078 (HECTc)
  
  697 - 1,069
  
  CDD
  cd00078 (HECTc)
3. SSF56204 (Hect, E3 ...)
  
  693 - 1,065
  
  SUPERFAMILY
  SSF56204 (Hect, E3 ligase catalytic domain)
4. SM00119 (hect_3)
  
  712 - 1,071
  
  SMART
  SM00119 (hect_3)
5. PS50237 (HECT)
  
  714 - 1,071
  
  PROSITE profiles
  PS50237 (HECT)

no IPR

Unintegrated signatures

CYTOPLASMIC_D... (C...)

333 - 424

PHOBIUS
CYTOPLASMIC_DOMAIN (Cytoplasmic domain)
Coil

1,070 - 1,071

COILS
Coil (Coil)
G3DSA:3.30.21...

855 - 919

GENE3D
G3DSA:3.30.2160.10 (G3DSA:3.30.2160.10)
G3DSA:3.30.24...

955 - 1,071

GENE3D
G3DSA:3.30.2410.10 (G3DSA:3.30.2410.10)
G3DSA:3.90.17...

657 - 854

GENE3D
G3DSA:3.90.1750.10 (G3DSA:3.90.1750.10)
NON_CYTOPLASM... (N...)

445 - 1,071

PHOBIUS
NON_CYTOPLASMIC_DOMAIN (Non cytoplasmic domain)

1 - 314

PHOBIUS
NON_CYTOPLASMIC_DOMAIN (Non cytoplasmic domain)
TRANSMEMBRANE (Tran...)

425 - 444

PHOBIUS
TRANSMEMBRANE (Transmembrane region)

315 - 332

PHOBIUS
TRANSMEMBRANE (Transmembrane region)
mobidb-lite (disord...)

610 - 638

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

catalytic cleft cd...

700R

catalytic cleft
700R, 729E, 743E, 823D, 1,002F, 1,033L, 1,034P, 1,037S, 1,038T, 1,039C, 1,040I, 1,041N, 1,061S, 1,068F
CDD
cd00078 (HECTc)

729E

catalytic cleft
700R, 729E, 743E, 823D, 1,002F, 1,033L, 1,034P, 1,037S, 1,038T, 1,039C, 1,040I, 1,041N, 1,061S, 1,068F
CDD
cd00078 (HECTc)

743E

catalytic cleft
700R, 729E, 743E, 823D, 1,002F, 1,033L, 1,034P, 1,037S, 1,038T, 1,039C, 1,040I, 1,041N, 1,061S, 1,068F
CDD
cd00078 (HECTc)

823D

catalytic cleft
700R, 729E, 743E, 823D, 1,002F, 1,033L, 1,034P, 1,037S, 1,038T, 1,039C, 1,040I, 1,041N, 1,061S, 1,068F
CDD
cd00078 (HECTc)

1,002F

catalytic cleft
700R, 729E, 743E, 823D, 1,002F, 1,033L, 1,034P, 1,037S, 1,038T, 1,039C, 1,040I, 1,041N, 1,061S, 1,068F
CDD
cd00078 (HECTc)

1,033L

catalytic cleft
700R, 729E, 743E, 823D, 1,002F, 1,033L, 1,034P, 1,037S, 1,038T, 1,039C, 1,040I, 1,041N, 1,061S, 1,068F
CDD
cd00078 (HECTc)

1,034P

catalytic cleft
700R, 729E, 743E, 823D, 1,002F, 1,033L, 1,034P, 1,037S, 1,038T, 1,039C, 1,040I, 1,041N, 1,061S, 1,068F
CDD
cd00078 (HECTc)

1,037S

catalytic cleft
700R, 729E, 743E, 823D, 1,002F, 1,033L, 1,034P, 1,037S, 1,038T, 1,039C, 1,040I, 1,041N, 1,061S, 1,068F
CDD
cd00078 (HECTc)

1,038T

catalytic cleft
700R, 729E, 743E, 823D, 1,002F, 1,033L, 1,034P, 1,037S, 1,038T, 1,039C, 1,040I, 1,041N, 1,061S, 1,068F
CDD
cd00078 (HECTc)

1,039C

catalytic cleft
700R, 729E, 743E, 823D, 1,002F, 1,033L, 1,034P, 1,037S, 1,038T, 1,039C, 1,040I, 1,041N, 1,061S, 1,068F
CDD
cd00078 (HECTc)

1,040I

catalytic cleft
700R, 729E, 743E, 823D, 1,002F, 1,033L, 1,034P, 1,037S, 1,038T, 1,039C, 1,040I, 1,041N, 1,061S, 1,068F
CDD
cd00078 (HECTc)

1,041N

catalytic cleft
700R, 729E, 743E, 823D, 1,002F, 1,033L, 1,034P, 1,037S, 1,038T, 1,039C, 1,040I, 1,041N, 1,061S, 1,068F
CDD
cd00078 (HECTc)

1,061S

catalytic cleft
700R, 729E, 743E, 823D, 1,002F, 1,033L, 1,034P, 1,037S, 1,038T, 1,039C, 1,040I, 1,041N, 1,061S, 1,068F
CDD
cd00078 (HECTc)

1,068F

catalytic cleft
700R, 729E, 743E, 823D, 1,002F, 1,033L, 1,034P, 1,037S, 1,038T, 1,039C, 1,040I, 1,041N, 1,061S, 1,068F
CDD
cd00078 (HECTc)
E2 interaction sit...

853I

E2 interaction site
853I, 856N, 857I, 859K, 860L, 863Y, 871F, 873L, 877I, 878D, 880N, 886Q, 891L, 908Y, 912V
CDD
cd00078 (HECTc)

856N

E2 interaction site
853I, 856N, 857I, 859K, 860L, 863Y, 871F, 873L, 877I, 878D, 880N, 886Q, 891L, 908Y, 912V
CDD
cd00078 (HECTc)

857I

E2 interaction site
853I, 856N, 857I, 859K, 860L, 863Y, 871F, 873L, 877I, 878D, 880N, 886Q, 891L, 908Y, 912V
CDD
cd00078 (HECTc)

859K

E2 interaction site
853I, 856N, 857I, 859K, 860L, 863Y, 871F, 873L, 877I, 878D, 880N, 886Q, 891L, 908Y, 912V
CDD
cd00078 (HECTc)

860L

E2 interaction site
853I, 856N, 857I, 859K, 860L, 863Y, 871F, 873L, 877I, 878D, 880N, 886Q, 891L, 908Y, 912V
CDD
cd00078 (HECTc)

863Y

E2 interaction site
853I, 856N, 857I, 859K, 860L, 863Y, 871F, 873L, 877I, 878D, 880N, 886Q, 891L, 908Y, 912V
CDD
cd00078 (HECTc)

871F

E2 interaction site
853I, 856N, 857I, 859K, 860L, 863Y, 871F, 873L, 877I, 878D, 880N, 886Q, 891L, 908Y, 912V
CDD
cd00078 (HECTc)

873L

E2 interaction site
853I, 856N, 857I, 859K, 860L, 863Y, 871F, 873L, 877I, 878D, 880N, 886Q, 891L, 908Y, 912V
CDD
cd00078 (HECTc)

877I

E2 interaction site
853I, 856N, 857I, 859K, 860L, 863Y, 871F, 873L, 877I, 878D, 880N, 886Q, 891L, 908Y, 912V
CDD
cd00078 (HECTc)

878D

E2 interaction site
853I, 856N, 857I, 859K, 860L, 863Y, 871F, 873L, 877I, 878D, 880N, 886Q, 891L, 908Y, 912V
CDD
cd00078 (HECTc)

880N

E2 interaction site
853I, 856N, 857I, 859K, 860L, 863Y, 871F, 873L, 877I, 878D, 880N, 886Q, 891L, 908Y, 912V
CDD
cd00078 (HECTc)

886Q

E2 interaction site
853I, 856N, 857I, 859K, 860L, 863Y, 871F, 873L, 877I, 878D, 880N, 886Q, 891L, 908Y, 912V
CDD
cd00078 (HECTc)

891L

E2 interaction site
853I, 856N, 857I, 859K, 860L, 863Y, 871F, 873L, 877I, 878D, 880N, 886Q, 891L, 908Y, 912V
CDD
cd00078 (HECTc)

908Y

E2 interaction site
853I, 856N, 857I, 859K, 860L, 863Y, 871F, 873L, 877I, 878D, 880N, 886Q, 891L, 908Y, 912V
CDD
cd00078 (HECTc)

912V

E2 interaction site
853I, 856N, 857I, 859K, 860L, 863Y, 871F, 873L, 877I, 878D, 880N, 886Q, 891L, 908Y, 912V
CDD
cd00078 (HECTc)

Protein sequence

>MCA_01711_1
MMGMNFTGQYKKARHINLGGRNARNATTANREALLRKAQADRERRERAVVEERSAIRIQSFFRGRSEAYKHRLQFGSHWA
PVMYNDDSVDAFLKVFNEFLYFFPKTYKSLDNSLQQIQQFESFLKKTDNSGTPMLSKIPQETLHRYLTRLTHFNDLAQIK
DLVSVKPDDKNNYFEILLSMLEIFYTVLPYVSFNVQPVLSKVLKYSILNHYIRESPTAVDRLRDISLKILKNQITSIPFD
SLTDGFIGIFITPNLHFFFTPQDFFDILPSELKLASTRPPSFYRNRNSFPDHEIVWAVSNFVHFFKNVYIKNQPFLFHST
LSILLGLSSFSLDSKKSSNSTNTSPDINEQQDEKLLENIASLFSNPFIQEQFDYITDRDFVSNLLELFIKPFADSNSITI
TNELVVFSQTITSLLSLKPSRKVQTLFTITLSNYPVISMLWLALQQSELYNTVLNQNLRIPELITIIDKNDNVLDVLFLF
MNLCQFWLIVATDSEFHGSVKNNGINGLERDQVQNLAQFLKKLCFILIWHGASADKSDFKFALISKNTAIYDILTGVMKS
IYARDSRRQFLPNDFWLMTNAFEMDHFVKSVAQEQRELFEWEEEEKLERLMREKNRDDDDDDDDEEEKKPKPEPTTPVSK
LVFAPRLELLREAPFFIPFDTRLKVFQEFITQMRNSDERLNTHFFDSFGRILKPIGKIRRDHLLEDAFKAFNALDSDFKQ
EVTVVFLNEHGAELGVGPGVTKEFLIQVSQEGFKPSEDGEYHIDTKRGLFSATKDHLLYPNPLLGISSKYSNLTKEERDD
GLTYMMFLGKIIGKCLFENILVDVEFAPFFLQKWTLPNFKNSFNDLYSLDPEIYDNIIKLCNYPGNVETDFMLDFTIDQN
VGHGKQATIELIPGGSKIPVTNANRLEYAHLVANYKLNAALYQQTKMFYEGLSMIIPPRWLNMFNARELQMLIAGRPSVF
DVDDLRANTVLLGFKESDETIQNFWEVLHELTDEQKSQFLKFVTSAPKAPLLGFRALTPNFAIRNAGDDRNRLPTASTCI
NLLKLSDMKSKELLKQKLLESIANGIGFELS

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0004842 ubiquitin-protein transferase activity

Cellular Component

None predicted.

Filter view on

Entry type

Status

Per-residue features

MCA_01711_1

Protein function

Protein alignments

Mitochondrial localization by mitoprotII

Protein family membership

Domains and repeats

Detailed signature matches

Residue annotation

Protein sequence

GO term prediction

Biological Process

Molecular Function

Cellular Component