MIA_00115_1

Protein

Length

1,300 amino acids

Protein function

EGGNOG:	0QE93	FG10511.1	nucleotide exchange factor
SGD closest match:	S000000039	CDC24	Cell division control protein 24
CGD closest match:	CAL0000190069	CDC24	Rho family guanine nucleotide exchange factor

Protein alignments

	%id	Aln length	E-value
MCA_04659_1	51.292%	774	0.0	MCA_04659_1
A0A0J9XJZ2_GEOCN	60.580%	586	0.0	Similar to Saccharomyces cerevisiae YAL041W CDC24 Guanine nucleotide exchange factor (GEF or GDP-release factor) for Cdc42p OS=Geotrichum candidum GN=BN980_GECA24s00758g PE=4 SV=1
UniRef50_A0A0J9XJZ2	60.580%	586	0.0	Similar to Saccharomyces cerevisiae YAL041W CDC24 Guanine nucleotide exchange factor (GEF or GDP-release factor) for Cdc42p n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9XJZ2_GEOCN
A0A167FRH5_9ASCO	60.177%	452	0.0	Rho family guanine nucleotide exchange factor CDC24 OS=Sugiyamaella lignohabitans GN=CDC24 PE=4 SV=1
A0A060T2M6_BLAAD	53.744%	454	2.31e-164	ARAD1A02662p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1A02662g PE=4 SV=1
Q6CBW7_YARLI	44.068%	590	1.18e-153	YALI0C14828p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_C14828g PE=4 SV=1
A0A1D8PNB5_CANAL	34.884%	602	7.37e-97	Rho family guanine nucleotide exchange factor OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=CDC24 PE=4 SV=1
A0A1E3PDB6_9ASCO	48.160%	326	1.14e-85	Uncharacterized protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_84393 PE=4 SV=1
CDC24_YEAST	35.225%	511	1.33e-85	Cell division control protein 24 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=CDC24 PE=1 SV=2
A0A1E4THH4_9ASCO	35.520%	442	7.72e-80	Uncharacterized protein (Fragment) OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_14730 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.6343
Predicted cleavage: 78

Protein family membership

None predicted.

Domains and repeats

Domain
278 - 364
Cdc24/Scd1, N-terminal (IPR010481)
567 - 739
PH domain-like (IPR011993)
Rho guanine nucleotide exchange factor Cdc24/Scd1, PH domain (IPR033511)
Domain
380 - 587
Dbl homology (DH) domain (IPR000219)
1,181 - 1,258
PB1 domain (IPR000270)

1 200 400 600 800 1000 1200 1300

Detailed signature matches

IPR010481

Cdc24/Scd1, N-terminal
1. PF06395 (CDC24)
  
  278 - 364
  
  Pfam
  PF06395 (CDC24)
IPR000219

Dbl homology (DH) domain
1. SSF48065 (DBL homol...)
  
  380 - 587
  
  SUPERFAMILY
  SSF48065 (DBL homology domain (DH-domain))
2. PF00621 (RhoGEF)
  
  396 - 564
  
  Pfam
  PF00621 (RhoGEF)
3. PS50010 (DH_2)
  
  392 - 566
  
  PROSITE profiles
  PS50010 (DH_2)
4. SM00325 (RhoGEF_3)
  
  396 - 565
  
  SMART
  SM00325 (RhoGEF_3)
IPR011993

PH domain-like
1. G3DSA:2.30.29.30
  
  586 - 739
  
  GENE3D
  G3DSA:2.30.29.30 (G3DSA:2.30.29.30)
2. SSF50729 (PH domain...)
  
  585 - 711
  
  SUPERFAMILY
  SSF50729 (PH domain-like)
IPR033511

Rho guanine nucleotide exchange factor Cdc24/Scd1, PH domain
1. cd13246 (PH_Scd1)
  
  567 - 711
  
  CDD
  cd13246 (PH_Scd1)
IPR000270

PB1 domain
1. PS51745 (PB1)
  
  1,181 - 1,258
  
  PROSITE profiles
  PS51745 (PB1)
2. PF00564 (PB1)
  
  1,182 - 1,250
  
  Pfam
  PF00564 (PB1)
3. SM00666 (PB1_new)
  
  1,181 - 1,258
  
  SMART
  SM00666 (PB1_new)
IPR001331

Guanine-nucleotide dissociation stimulator, CDC24, conserved site
1. PS00741 (DH_1)
  
  514 - 539
  
  PROSITE patterns
  PS00741 (DH_1)

no IPR

Unintegrated signatures

G3DSA:1.20.90...

390 - 585

GENE3D
G3DSA:1.20.900.10 (G3DSA:1.20.900.10)
G3DSA:3.10.20.90

1,170 - 1,255

GENE3D
G3DSA:3.10.20.90 (G3DSA:3.10.20.90)
PF15411 (PH_10)

593 - 707

Pfam
PF15411 (PH_10)
SSF54277 (CAD & PB1...)

1,173 - 1,250

SUPERFAMILY
SSF54277 (CAD & PB1 domains)
cd00160 (RhoGEF)

393 - 564

CDD
cd00160 (RhoGEF)
cd05992 (PB1)

1,182 - 1,251

CDD
cd05992 (PB1)
mobidb-lite (disord...)

903 - 970

MobiDB Lite
mobidb-lite (disorder_prediction)

99 - 137

MobiDB Lite
mobidb-lite (disorder_prediction)

1,130 - 1,178

MobiDB Lite
mobidb-lite (disorder_prediction)

147 - 166

MobiDB Lite
mobidb-lite (disorder_prediction)

1,260 - 1,279

MobiDB Lite
mobidb-lite (disorder_prediction)

1,049 - 1,092

MobiDB Lite
mobidb-lite (disorder_prediction)

1 - 55

MobiDB Lite
mobidb-lite (disorder_prediction)

792 - 825

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

GTPase interaction...

399E

GTPase interaction site
399E, 403T, 495T, 515P, 516S, 519I, 520K, 522I, 523Q, 526C, 527K, 530L, 531L, 534Q, 560T, 564N
CDD
cd00160 (RhoGEF)

403T

GTPase interaction site
399E, 403T, 495T, 515P, 516S, 519I, 520K, 522I, 523Q, 526C, 527K, 530L, 531L, 534Q, 560T, 564N
CDD
cd00160 (RhoGEF)

495T

GTPase interaction site
399E, 403T, 495T, 515P, 516S, 519I, 520K, 522I, 523Q, 526C, 527K, 530L, 531L, 534Q, 560T, 564N
CDD
cd00160 (RhoGEF)

515P

GTPase interaction site
399E, 403T, 495T, 515P, 516S, 519I, 520K, 522I, 523Q, 526C, 527K, 530L, 531L, 534Q, 560T, 564N
CDD
cd00160 (RhoGEF)

516S

GTPase interaction site
399E, 403T, 495T, 515P, 516S, 519I, 520K, 522I, 523Q, 526C, 527K, 530L, 531L, 534Q, 560T, 564N
CDD
cd00160 (RhoGEF)

519I

GTPase interaction site
399E, 403T, 495T, 515P, 516S, 519I, 520K, 522I, 523Q, 526C, 527K, 530L, 531L, 534Q, 560T, 564N
CDD
cd00160 (RhoGEF)

520K

GTPase interaction site
399E, 403T, 495T, 515P, 516S, 519I, 520K, 522I, 523Q, 526C, 527K, 530L, 531L, 534Q, 560T, 564N
CDD
cd00160 (RhoGEF)

522I

GTPase interaction site
399E, 403T, 495T, 515P, 516S, 519I, 520K, 522I, 523Q, 526C, 527K, 530L, 531L, 534Q, 560T, 564N
CDD
cd00160 (RhoGEF)

523Q

GTPase interaction site
399E, 403T, 495T, 515P, 516S, 519I, 520K, 522I, 523Q, 526C, 527K, 530L, 531L, 534Q, 560T, 564N
CDD
cd00160 (RhoGEF)

526C

GTPase interaction site
399E, 403T, 495T, 515P, 516S, 519I, 520K, 522I, 523Q, 526C, 527K, 530L, 531L, 534Q, 560T, 564N
CDD
cd00160 (RhoGEF)

527K

GTPase interaction site
399E, 403T, 495T, 515P, 516S, 519I, 520K, 522I, 523Q, 526C, 527K, 530L, 531L, 534Q, 560T, 564N
CDD
cd00160 (RhoGEF)

530L

GTPase interaction site
399E, 403T, 495T, 515P, 516S, 519I, 520K, 522I, 523Q, 526C, 527K, 530L, 531L, 534Q, 560T, 564N
CDD
cd00160 (RhoGEF)

531L

GTPase interaction site
399E, 403T, 495T, 515P, 516S, 519I, 520K, 522I, 523Q, 526C, 527K, 530L, 531L, 534Q, 560T, 564N
CDD
cd00160 (RhoGEF)

534Q

GTPase interaction site
399E, 403T, 495T, 515P, 516S, 519I, 520K, 522I, 523Q, 526C, 527K, 530L, 531L, 534Q, 560T, 564N
CDD
cd00160 (RhoGEF)

560T

GTPase interaction site
399E, 403T, 495T, 515P, 516S, 519I, 520K, 522I, 523Q, 526C, 527K, 530L, 531L, 534Q, 560T, 564N
CDD
cd00160 (RhoGEF)

564N

GTPase interaction site
399E, 403T, 495T, 515P, 516S, 519I, 520K, 522I, 523Q, 526C, 527K, 530L, 531L, 534Q, 560T, 564N
CDD
cd00160 (RhoGEF)
PB1 interaction cd...

1,183T

PB1 interaction
1,183T, 1,185K, 1,191D, 1,192T, 1,193F, 1,209R
CDD
cd05992 (PB1)

1,185K

PB1 interaction
1,183T, 1,185K, 1,191D, 1,192T, 1,193F, 1,209R
CDD
cd05992 (PB1)

1,191D

PB1 interaction
1,183T, 1,185K, 1,191D, 1,192T, 1,193F, 1,209R
CDD
cd05992 (PB1)

1,192T

PB1 interaction
1,183T, 1,185K, 1,191D, 1,192T, 1,193F, 1,209R
CDD
cd05992 (PB1)

1,193F

PB1 interaction
1,183T, 1,185K, 1,191D, 1,192T, 1,193F, 1,209R
CDD
cd05992 (PB1)

1,209R

PB1 interaction
1,183T, 1,185K, 1,191D, 1,192T, 1,193F, 1,209R
CDD
cd05992 (PB1)
PB1 interaction su...

1,183T

PB1 interaction surface
1,183T, 1,185K, 1,192T, 1,193F, 1,194L, 1,209R, 1,213K
CDD
cd05992 (PB1)

1,185K

PB1 interaction surface
1,183T, 1,185K, 1,192T, 1,193F, 1,194L, 1,209R, 1,213K
CDD
cd05992 (PB1)

1,192T

PB1 interaction surface
1,183T, 1,185K, 1,192T, 1,193F, 1,194L, 1,209R, 1,213K
CDD
cd05992 (PB1)

1,193F

PB1 interaction surface
1,183T, 1,185K, 1,192T, 1,193F, 1,194L, 1,209R, 1,213K
CDD
cd05992 (PB1)

1,194L

PB1 interaction surface
1,183T, 1,185K, 1,192T, 1,193F, 1,194L, 1,209R, 1,213K
CDD
cd05992 (PB1)

1,209R

PB1 interaction surface
1,183T, 1,185K, 1,192T, 1,193F, 1,194L, 1,209R, 1,213K
CDD
cd05992 (PB1)

1,213K

PB1 interaction surface
1,183T, 1,185K, 1,192T, 1,193F, 1,194L, 1,209R, 1,213K
CDD
cd05992 (PB1)
PB1 interaction su...

1,185K

PB1 interaction surface
1,185K, 1,213K
CDD
cd05992 (PB1)

1,213K

PB1 interaction surface
1,185K, 1,213K
CDD
cd05992 (PB1)
PB1 interaction su...

1,231D

PB1 interaction surface
1,231D, 1,244D
CDD
cd05992 (PB1)

1,244D

PB1 interaction surface
1,231D, 1,244D
CDD
cd05992 (PB1)
PB1 interaction si...

1,231D

PB1 interaction site
1,231D, 1,244D
CDD
cd05992 (PB1)

1,244D

PB1 interaction site
1,231D, 1,244D
CDD
cd05992 (PB1)

Protein sequence

>MIA_00115_1
MTYSGLKQSISNSSPSRSSNNNTLSNSTPSITPISSNSQYSNPASHYHQQTSSSLFHTHSHTLGHLRSHSSSARSRSNSV
SAANLFHFGHHLFSTDSSTTTSSSSSSFSSSSTPSSSPSSQAPSPSPSPGPSLSLSISHPFSFSHSATIQTPTAPIPANV
PGNKKASESSSLFQIAAQIKDRLEDVPGIPPFLEMCIGNLPPQHLIQAQLQNTSSSTPDRNSAADLSLANVSTNSSISTA
VPQQSSAASASSSTTIQHTNSSLSLSSSAVIPVGMKIDPVTHLWQFFRLGSSLCALFNALQPKEPLEVAITNDVKRCKRS
VYDFLQGCKSELDYSDEDIFTISNVFSDNTTDLLKVIRTVKLVLDTLESRNLITPAKNKKSSISSLRPPRDKRDKVVEEI
LLTERKFVQDLELLHEYQCKLQDLGVLSADTIHNLFPSLNVLIDFQRRFLIGIEYHAHLPPEEQRFGSVFLNFQEGFEVY
EGFALHYKKACDIATSESAKLQPLKDILDPHYELPSFLIKPIQRICKYPLLLEQLVKYTPKDYPNYQEQVDALDAIRRVT
ASVNETKRRVENKEVVRDLSERLVDWKGHNVDDFGNLIHDGVFPVLKAGTEREYHLYLFENIILCCKEAPPSKKSMALST
KKNKVKRSSVLILKGRIYIAYITNVSAFVSNGYLLHIAWGRDDATDTGFFDIRFKHDEQLELWRSTIESLVAHYNDESNA
ADLIPVAPPHQQTLMHTQGYGALMLPSNGYLPTNSLQQYQQQQHQKQIQQQHLLHQQQQIFNAQSRQNSFVQSFGNSDVN
GTSYDDDDEDDMYITNNQGNSSNRQYDEDTLAALHQGLNTLSYPIDQAQQNTPTTNRSSSHSVQHLTNDLANTHVSSEGN
LRQAARQDSTASLSALHPAHHFQNMRSRASSIPPSQLGGNNANLANGTSKEVPSVLDSPETMLTPSLSPRAVKTGPPMNG
RGNGTAGGVSGGHLSIDGGISMGAGPSAAACLRHSVHDNNDYLLRGGVDRTSVSTVSSPFGPTGERLSMGTAAELGLAAN
SRTRAGSVSSVGLMAGGNGNGSSSSSSSSSNSSISSNFDQPSPLNGPSAHKNSISSIASMASTSSTMSTASSSSTSTMFL
PSLPAVSTTVPTAGGGLHGGVQNKTSTAATSDSARNSVSGSNGLSSNEANGVAQSPSKPQKITVKLHYLDDTFLLIMPAA
VTYAELLERVERKVRLCGNQTPSPLRIKYKDEDDDFVTMRSDEDIQMAMELKMCGVPGEGGGSDVDGASRNSTGDVEGSS
VSIIPGKLGSSRQDLIIWAA

GO term prediction

Biological Process

GO:0035023 regulation of Rho protein signal transduction
GO:0035556 intracellular signal transduction

Molecular Function

GO:0005085 guanyl-nucleotide exchange factor activity
GO:0005089 Rho guanyl-nucleotide exchange factor activity
GO:0005515 protein binding

Cellular Component