MIA_00086_1

Protein

Length

1,831 amino acids

Protein function

EGGNOG:	0PHUI	FG10913.1	carboxylase
SGD closest match:	S000000412	DUR1,2	Urea amidolyase
CGD closest match:	CAL0000184290	DUR1,2	Bifunctional urea carboxylase/allophanate hydrolase

Protein alignments

	%id	Aln length	E-value
MCA_03947_1	82.952%	1836	0.0	MCA_03947_1
A0A161HFE0_9ASCO	67.376%	1833	0.0	Bifunctional urea carboxylase/allophanate hydrolase OS=Sugiyamaella lignohabitans GN=DUR1,2 PE=4 SV=1
A0A1E3PSD7_9ASCO	66.576%	1843	0.0	Urea amidolyase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_48988 PE=4 SV=1
Q6C6Q4_YARLI	65.439%	1846	0.0	YALI0E07271p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E07271g PE=4 SV=1
A0A060TC69_BLAAD	64.834%	1837	0.0	ARAD1D39732p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D39732g PE=4 SV=1
A0A1E4T9N7_9ASCO	61.920%	1833	0.0	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_146769 PE=4 SV=1
A0A1D8PDC6_CANAL	61.801%	1843	0.0	Bifunctional urea carboxylase/allophanate hydrolase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=DUR1,2 PE=4 SV=1
UniRef50_A0A1E4SN07	61.372%	1851	0.0	Urea carboxylase n=1 Tax=Candida tanzawaensis NRRL Y-17324 TaxID=984487 RepID=A0A1E4SN07_9ASCO
DUR1_YEAST	60.087%	1849	0.0	Urea amidolyase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=DUR1,2 PE=1 SV=2
A0A0J9X8S2_GEOCN	42.032%	433	9.89e-109	Similar to Saccharomyces cerevisiae YBR208C DUR1,2 Urea amidolyase OS=Geotrichum candidum GN=BN980_GECA05s04498g PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0367

Protein family membership

Urea carboxylase (IPR014084)

Domains and repeats

Domain
6 - 603
Amidase signature domain (IPR023631)
Allophanate hydrolase (IPR014085)
735 - 818
ATP-grasp fold, subdomain 1 (IPR013815)
1,057 - 1,392
Carboxyltransferase domain, subdomain A and B (IPR003778)
1,433 - 1,663
Carboxyltransferase domain, subdomain C and D (IPR003833)
Domain
588 - 724
Pre-ATP-grasp domain (IPR016185)
Biotin carboxylase-like, N-terminal domain (IPR005481)
727 - 929
ATP-grasp fold (IPR011761)
Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domain (IPR005479)
939 - 1,052
Rudiment single hybrid motif (IPR011054)
Biotin carboxylase, C-terminal (IPR005482)
1,250 - 1,376
Cyclophilin-like domain (IPR029000)
1,521 - 1,681
Cyclophilin-like domain (IPR029000)
1,749 - 1,830
Single hybrid motif (IPR011053)
Biotin/lipoyl attachment (IPR000089)
Domain
612 - 1,053
Biotin carboxylation domain (IPR011764)
1,417 - 1,515
Arginine repressor C-terminal-like domain (IPR024946)

1 200 400 600 800 1000 1200 1400 1600 1831

Detailed signature matches

IPR014084

Urea carboxylase
1. TIGR02712
  
  613 - 1,830
  
  TIGRFAMs
  TIGR02712 (TIGR02712)
IPR023631

Amidase signature domain
1. SSF75304 (Amidase s...)
  
  18 - 441
  
  SUPERFAMILY
  SSF75304 (Amidase signature (AS) enzymes)
2. G3DSA:3.90.13...
  
  6 - 454
  
  GENE3D
  G3DSA:3.90.1300.10 (G3DSA:3.90.1300.10)
3. PF01425 (Amidase)
  
  43 - 432
  
  Pfam
  PF01425 (Amidase)
IPR014085

Allophanate hydrolase
1. TIGR02713
  
  40 - 603
  
  TIGRFAMs
  TIGR02713 (TIGR02713)
IPR016185

Pre-ATP-grasp domain
1. SSF52440 (PreATP-gr...)
  
  588 - 724
  
  SUPERFAMILY
  SSF52440 (PreATP-grasp domain)
IPR011764

Biotin carboxylation domain
1. PS50979 (BC)
  
  612 - 1,053
  
  PROSITE profiles
  PS50979 (BC)
IPR005481

Biotin carboxylase-like, N-terminal domain
1. PF00289 (Biotin_carb_N)
  
  613 - 721
  
  Pfam
  PF00289 (Biotin_carb_N)
IPR011761

ATP-grasp fold
1. PS50975 (ATP_GRASP)
  
  731 - 928
  
  PROSITE profiles
  PS50975 (ATP_GRASP)
IPR005479

Carbamoyl-phosphate synthetase large subunit-like, ATP-binding domain
1. PF02786 (CPSase_L_D2)
  
  727 - 929
  
  Pfam
  PF02786 (CPSase_L_D2)
2. PS00867 (CPSASE_2)
  
  897 - 904
  
  PROSITE patterns
  PS00867 (CPSASE_2)
3. PS00866 (CPSASE_1)
  
  763 - 777
  
  PROSITE patterns
  PS00866 (CPSASE_1)
IPR013815

ATP-grasp fold, subdomain 1
1. G3DSA:3.30.14...
  
  735 - 818
  
  GENE3D
  G3DSA:3.30.1490.20 (G3DSA:3.30.1490.20)
IPR011054

Rudiment single hybrid motif
1. SSF51246 (Rudiment ...)
  
  939 - 1,052
  
  SUPERFAMILY
  SSF51246 (Rudiment single hybrid motif)
IPR005482

Biotin carboxylase, C-terminal
1. SM00878 (Biotin_car...)
  
  945 - 1,049
  
  SMART
  SM00878 (Biotin_carb_C_2)
2. PF02785 (Biotin_carb_C)
  
  945 - 1,049
  
  Pfam
  PF02785 (Biotin_carb_C)
IPR003778

Carboxyltransferase domain, subdomain A and B
1. PF02626 (CT_A_B)
  
  1,081 - 1,369
  
  Pfam
  PF02626 (CT_A_B)
2. TIGR00724
  
  1,057 - 1,392
  
  TIGRFAMs
  TIGR00724 (TIGR00724)
3. SM00797 (ahs2)
  
  1,080 - 1,386
  
  SMART
  SM00797 (ahs2)
IPR029000

Cyclophilin-like domain
1. SSF50891 (Cyclophil...)
  
  1,550 - 1,679
  
  SUPERFAMILY
  SSF50891 (Cyclophilin-like)
  
  1,250 - 1,376
  
  SUPERFAMILY
  SSF50891 (Cyclophilin-like)
2. G3DSA:2.40.10...
  
  1,521 - 1,681
  
  GENE3D
  G3DSA:2.40.100.10 (G3DSA:2.40.100.10)
IPR024946

Arginine repressor C-terminal-like domain
1. G3DSA:3.30.13...
  
  1,417 - 1,515
  
  GENE3D
  G3DSA:3.30.1360.40 (G3DSA:3.30.1360.40)
IPR003833

Carboxyltransferase domain, subdomain C and D
1. SM00796 (ahs1)
  
  1,433 - 1,663
  
  SMART
  SM00796 (ahs1)
2. PF02682 (CT_C_D)
  
  1,434 - 1,651
  
  Pfam
  PF02682 (CT_C_D)
IPR011053

Single hybrid motif
1. SSF51230 (Single hy...)
  
  1,757 - 1,830
  
  SUPERFAMILY
  SSF51230 (Single hybrid motif)
IPR000089

Biotin/lipoyl attachment
1. PS50968 (BIOTINYL_L...)
  
  1,749 - 1,830
  
  PROSITE profiles
  PS50968 (BIOTINYL_LIPOYL)
2. PF00364 (Biotin_lipoyl)
  
  1,770 - 1,828
  
  Pfam
  PF00364 (Biotin_lipoyl)
IPR001882

Biotin-binding site
1. PS00188 (BIOTIN)
  
  1,786 - 1,803
  
  PROSITE patterns
  PS00188 (BIOTIN)

no IPR

Unintegrated signatures

Coil

1,727 - 1,747

COILS
Coil (Coil)
G3DSA:2.40.50...

1,759 - 1,831

GENE3D
G3DSA:2.40.50.100 (G3DSA:2.40.50.100)
G3DSA:3.10.49...

470 - 612

GENE3D
G3DSA:3.10.490.10 (G3DSA:3.10.490.10)
G3DSA:3.30.47...

819 - 1,054

GENE3D
G3DSA:3.30.470.20 (G3DSA:3.30.470.20)
G3DSA:3.40.50.20

613 - 734

GENE3D
G3DSA:3.40.50.20 (G3DSA:3.40.50.20)
SSF160467 (PH0987 N...)

1,435 - 1,526

SUPERFAMILY
SSF160467 (PH0987 N-terminal domain-like)
SSF56059 (Glutathio...)

694 - 957

SUPERFAMILY
SSF56059 (Glutathione synthetase ATP-binding domain-like)
cd06850 (biotinyl_d...)

1,764 - 1,829

CDD
cd06850 (biotinyl_domain)

Residue annotation

carboxyltransferas...

1,786G

carboxyltransferase (CT) interaction site
1,786G, 1,795M, 1,796K, 1,797T, 1,804S
CDD
cd06850 (biotinyl_domain)

1,795M

carboxyltransferase (CT) interaction site
1,786G, 1,795M, 1,796K, 1,797T, 1,804S
CDD
cd06850 (biotinyl_domain)

1,796K

carboxyltransferase (CT) interaction site
1,786G, 1,795M, 1,796K, 1,797T, 1,804S
CDD
cd06850 (biotinyl_domain)

1,797T

carboxyltransferase (CT) interaction site
1,786G, 1,795M, 1,796K, 1,797T, 1,804S
CDD
cd06850 (biotinyl_domain)

1,804S

carboxyltransferase (CT) interaction site
1,786G, 1,795M, 1,796K, 1,797T, 1,804S
CDD
cd06850 (biotinyl_domain)
biotinylation site...

1,796K

biotinylation site
1,796K
CDD
cd06850 (biotinyl_domain)

Protein sequence

>MIA_00086_1
MVNTIGWTISQWTQYTTSTDPAEVLKTIQALVASFPKDDPAWISTASPELIKEQFDALTNKKLPLYAVPIAVKDNIDAKG
FATTAACPAYSYTPEKDATIVSLLRKAGAIVVGKTNLDQFATGLVGTRSPYGKTPCVFSDKHVSGGSSAGSGSVVGRGIV
PLALGTDTAGSGRVPAALNNIVGLKPTRGAFSTTGVVPACRSLDCPSLFTLNLQDAQLVFDIAAKFDPTDSYSRQLPNKL
LTSFGKAPVFAYPSEPKFYDDKYNPVLFQQALETLKSTGVKLVSTDFSILHKLANLLYEGPWVGERYAAIREFVTSRKDE
MDPVVYGIVKKAENFSAADCFDFEYKRMDILREIQEQFDQFDGLVVPTCPLNPTFADLEAEPVLVNSYQGTYTNFVNLAD
LSALAIPVGFRPDGLPNGITFISKKFNDYALLDLASKFEKANAANRTFVGALKDVPVAQGDLLHEGIPVTSPSVIPLVVV
GGHLSGMALNWQLQKVNATLVQKTKTSRNYRLYALPNTTPAKPGLRRILPSDSPLVGEEIIVEVWNVPAEEFGTFISMVP
HPLGIGSVELADGRWVKGFICEQAGFHDAEDITKFGGWRAYMASKLDPKTKPFETVLVANHGEIAVRIIKTLKKLGIKAV
SVFSEPDRYTQHVLDADDAVSLEGTTAAETYLNVDKIIKAAKDTGAQAIIPGYGFLSENADFAEACENNGIIFIGPSADA
IRALGLKHSAREIAERAGVPMVPGSGLIDTVEEAVAIAERIKYPVMVKSTAGGGGIGLQCVRNPEDLPKAFETVKHQGLS
YFNDSGVFIERNVENARHVEVQVFGDGRGGAIALGERDCSLQRRHQKIVEETPAPRLSDKTRSAMRDAAQSLVSTMKYKC
AGTVEFIYDEKNEEFYFLEVNARLQVEHTITEEVTGLDLVEWMLYIAADRPPAFTPVTPKGAAIEVRIYAENPVKDFLPS
PGQITDVTFPDWARVDTWVVKGSKISPEYDPTIAKMIVYGKDRDDAIQKMSKALDETVIYGPVTNIDYLRAIVNSEMFKE
AKVATKVLDSWTYTPSAIEITAPGSYTTVQDYPGRVKLWHIGVPPSGPMDSYAFRAANKIVGNDSKAPAIECTLVGPSLV
FHCDTVFALTGGLSPATLDDKPIEFWKPISVKAGSKLVIGKLSTGCRSYLAIRGGIDVPEYLGSRSTFALGTMGGFNGRV
IKFGDVLFLGNPEVSGCTLPAPIAEPMEIPSSLVPAVLATPPAAAGPTTWNVGVTVGPHGAPDFFAPEYIETFFSSEWKV
HYNSNRFGVRLVGPKPKWARKDGGEAGLHPSNAHDYVYSLGAVNFTGDEPVILTCDGPSLGGFVCAAVIVEAEMWKIGQV
KPGDLIKFVPITFEDAIALKNWQDKVLDSVSDSFVDEKPLEIVSEKLSSAVATASSAEPILFHQAATASTPKVVYRQAGD
RYVLVEYGENIMDLNLSYRVYKLTEAVFGHNTKGVVEMSAGVRSVLIEFSGKETTQEELLKTLISYESKLVATENWQVPS
RTIRLPLAFEDSKTLGAVERYKETIRSTAPWLPNNVDFIKEVNGLEDRNDVRDMLYSAKFLVLGLGDVFLGAPCAVPLDP
RHRLLGTKYNPSRTYTPNGTVGIGGMYMCIYTMESPGGYQLVGRTIPIWDKLTLGQQDRPWLLSQFDVVEYFAVSEEELD
VFCEQVENGQYHIEIEDSVFDHGKYVEWLDENKESIAAFAERQLEGNEEISRLIQVSNAELQAAQESGAGEDDSSKYSEN
AELVYAEVTGRFWKNMVVVGDKVEKGQGLVIIEAMKTEMVVPSSATGTVVKIVHKNGDIVEAGDLVAIVEP

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0003824 catalytic activity
GO:0004075 biotin carboxylase activity
GO:0005524 ATP binding
GO:0046872 metal ion binding

Cellular Component