Protein
MCA_06109_2
Length
540 amino acids
Gene name: THI6
Description: Thiamine biosynthetic bifunctional enzyme
Browser: contigD:3267620-3269312-
RNA-seq: read pairs 4914, FPKM 112.2, percentile rank 80.6% (100% = highest expression)
Protein function
| Annotation: | THI6 | Thiamine biosynthetic bifunctional enzyme | |
|---|---|---|---|
| KEGG: | K14154 | THI6 | thiamine-phosphate diphosphorylase / hydroxyethylthiazole kinase [EC:2.5.1.3 2.7.1.50] |
| EGGNOG: | 0PFZZ | FG07264.1 | thiamine biosynthetic bifunctional enzyme |
| SGD closest match: | S000006135 | THI6 | Thiamine biosynthetic bifunctional enzyme |
| CGD closest match: | CAL0000179143 | THI6 | Bifunctional hydroxyethylthiazole kinase/thiamine-phosphate diphosphorylase |
Protein alignments
| %id | Aln length | E-value | ||
|---|---|---|---|---|
| MIA_06371_1 | 56.80% | 537 | 0.0 | MIA_06371_1 |
| A0A1E3PP80_9ASCO | 43.45% | 550 | 6e-140 | Thiamine biosynthetic bifunctional enzyme OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_81786 PE=3 SV=1 |
| UniRef50_A0A1E3PP80 | 43.45% | 550 | 2e-136 | Thiamine biosynthetic bifunctional enzyme n=1 Tax=Nadsonia fulvescens var. elongata DSM 6958 TaxID=857566 RepID=A0A1E3PP80_9ASCO |
| A0A167D4I6_9ASCO | 44.85% | 553 | 1e-139 | Bifunctional hydroxyethylthiazole kinase/thiamine-phosphate diphosphorylase OS=Sugiyamaella lignohabitans GN=THI6 PE=3 SV=1 |
| A0A0J9X5W5_GEOCN | 42.91% | 564 | 1e-130 | Similar to Saccharomyces cerevisiae YPL214C THI6 Bifunctional enzyme with thiamine-phosphate pyrophosphorylase and 4-methyl-5-beta-hydroxyethylthiazole kinase activities OS=Geotrichum candidum GN=BN980_GECA03s03882g PE=3 SV=1 |
| A0A060T700_BLAAD | 42.78% | 540 | 5e-129 | ARAD1B18722p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1B18722g PE=3 SV=1 |
| Q6CBT9_YARLI | 41.48% | 528 | 3e-111 | YALI0C15554p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_C15554g PE=3 SV=1 |
| Q5AEJ1_CANAL | 40.07% | 534 | 3e-109 | Bifunctional hydroxyethylthiazole kinase/thiamine-phosphate diphosphorylase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=THI6 PE=3 SV=1 |
| A0A1E4TCF9_9ASCO | 40.43% | 512 | 1e-102 | Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_40887 PE=3 SV=1 |
| THI6_YEAST | 36.59% | 563 | 9e-96 | Thiamine biosynthetic bifunctional enzyme OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=THI6 PE=3 SV=1 |
Mitochondrial localization by mitoprotII
Probability of mitochondrial location: 0.1360
Protein family membership
- Thiamine phosphate synthase (IPR034291)
- Hydroxyethylthiazole kinase (IPR000417)
Domains and repeats
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Domain
1
50
100
150
200
250
300
350
400
450
500
540
Detailed signature matches
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-
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SSF53613 (Ribokinas...)
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Residue annotation
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thiamine phosphate...
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active site cd00564
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pyrophosphate bind...
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substrate binding ...
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multimerization in...
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ATP binding site c...
Protein sequence
>MCA_06109_2 MFSKAEIDYSLYLVTDSLLIPEGSDLFTQVEKALSGGSVTIVQHREKNIETNDFIQRATKLHEITKRYSVPLIINDRADI AVAIDAEGVHVGQNDLDVARVRKIIGNDKIVGVSVNNKEELAKAIQDQADYVGIGAVYGTKTKALEQSPIVPTGVKELLF EAASLSKTHLPAVAIGGINSSNIFKTIYLSSSPEDNIKLDGVAVVSAIMASKTPDIAANELKHLLKQSFESSFTSKNTSL SQMTLDDIKKATGILTAKISQIKPLVHHITNDVVKNFSANVTIAIGASPVMSECPEDFCDLSAVPTCGCLINMGTPTVEG IKMYKKSIDEYNKRGKTLVFDPVGAGATKLRTDSCQELLEHGIFDAVKANDGEIFSAAQYEGGSKSVGVDSVGKSSNEER YKAAFKIAKKHRTVVLATGEEDVLVDEQGNCLIFSNGSEYLGKITGSGCSLGSLITTYIAADKGLAREDRDYKPVGPFIA TAAALSLYTLASEKAAEKPQCLGPGTFIPNFIDELYLLQKAAANGNTEWLSNLKVTHYET
GO term prediction
Biological Process
GO:0009228 thiamine biosynthetic process
Molecular Function
GO:0003824 catalytic activity
GO:0004417 hydroxyethylthiazole kinase activity
GO:0004789 thiamine-phosphate diphosphorylase activity
Cellular Component
None predicted.