Protein

MCA_05937_1

Length
464 amino acids

Gene name: RAD18

Description: Postreplication repair E3 ubiquitin-protein ligase RAD18

Browser: contigD:2805859-2807254+

RNA-seq: read pairs 232, FPKM 6.2, percentile rank 19.3% (100% = highest expression)

Protein function

Annotation:RAD18Postreplication repair E3 ubiquitin-protein ligase RAD18
KEGG:K10627RAD18 E3 ubiquitin-protein ligase RAD18 [EC:2.3.2.27]
EGGNOG:0PJ1FRAD18E3 RING-finger protein, member of the UBC2 RAD6 epistasis group. Associates to the E2 ubiquitin conjugating enzyme UBC2 RAD6 to form the UBC2-RAD18 ubiquitin ligase complex involved in postreplicative repair (PRR) of damaged DNA
SGD closest match:S000000662RAD18Postreplication repair E3 ubiquitin-protein ligase RAD18
CGD closest match:CAL0000182971RAD18Postreplication repair E3 ubiquitin-protein ligase RAD18

Protein alignments

%idAln lengthE-value
MIA_00721_137.35%3409e-68MIA_00721_1
UniRef50_A0A0S6XB4834.32%3036e-42Uncharacterized protein n=1 Tax=fungal sp. No.11243 TaxID=1603295 RepID=A0A0S6XB48_9FUNG
A0A0J9XIQ8_GEOCN34.30%3096e-45Similar to Saccharomyces cerevisiae YCR066W RAD18 E3 ubiqutin ligase OS=Geotrichum candidum GN=BN980_GECA17s01110g PE=4 SV=1
A0A060T995_BLAAD34.87%3047e-43ARAD1C42284p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C42284g PE=4 SV=1
A0A1E3PDZ3_9ASCO30.77%2995e-28Uncharacterized protein (Fragment) OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_8231 PE=4 SV=1
RAD18_YEAST27.46%3462e-26Postreplication repair E3 ubiquitin-protein ligase RAD18 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RAD18 PE=1 SV=1
RAD18_YARLI47.17%1062e-26Postreplication repair E3 ubiquitin-protein ligase RAD18 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=RAD18 PE=3 SV=1
RAD18_CANAL39.39%993e-21Postreplication repair E3 ubiquitin-protein ligase RAD18 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=RAD18 PE=3 SV=2
A0A1E4TC30_9ASCO24.27%3752e-15Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_3829 PE=4 SV=1
A0A167DZN3_9ASCO28.97%1455e-11E3 ubiquitin-protein ligase RAD18 OS=Sugiyamaella lignohabitans GN=RAD18 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0163

Protein family membership

Domains and repeats

  1. Domain
1 50 100 150 200 250 300 350 400 464

Detailed signature matches

  1. Family IPR004580
    DNA repair protein, Rad18
    1. TIGR00599
  2. Domain IPR013083
    Zinc finger, RING/FYVE/PHD-type
    1. G3DSA:3.30.40.10
  3. Domain IPR001841
    Zinc finger, RING-type
    1. SM00184 (ring_2)
    2. PS50089 (ZF_RING_2)
  4. SITE IPR017907
    Zinc finger, RING-type, conserved site
    1. PS00518 (ZF_RING_1)
Unintegrated signatures no IPR
Unintegrated signatures
  1. Coil
  2. PF13923 (zf-C3HC4_2)
  3. SSF57850 (RING/U-box)
  4. cd16529 (RING-HC_RAD18)
  5. mobidb-lite (disord...)

Residue annotation

  1. Zn binding site cd...
  2. homodimer interfac...

Protein sequence

>MCA_05937_1
MTLSTANITDSSDWAGTKIPELSQLDEALRCDICKEFMTAPMLTSCFHSFCSLCIRRVLVKEGRCPTCRTRCSEAQLRKN
TTVEVCVNIFERLRGNLIKIVSKNEESETPVLEPSSESSQSPAQATDHSQNDNIRRTRSSTRKTSGQSNPETIPPGHAKC
PVCDKVLKITDIEGKHLPLCLARSQPSLGNSSGESSEYEETSSRKSSTKILELGKSRSSKEAEQLKQRIPRSNELRISLP
KLRTRLAKYGIPTNGNTKQELLDRETEWINLWNANADSSHPHSKKELLEELDRWEKAQKSTRESAKSSRANMFMAASADT
QKIASTASNESSNQDNTLGEPSENTNNQEPNVNESKGIKRSLEEDSPNTEENQKEEDKKKKKKLKKNKKAKKTKIDIDTI
KPITDLKKVDRGVYSYVYNSAFKELEMEAKERLAKAKATQLEEAKKAQAVTENVKAEKTEDVQE

GO term prediction

Biological Process

GO:0006281 DNA repair

Molecular Function

GO:0003684 damaged DNA binding

Cellular Component

GO:0005634 nucleus