Protein

MCA_05823_1

Length
280 amino acids

Gene name: MET8

Description: Siroheme biosynthesis protein MET8

Browser: contigD:2429963-2430806-

RNA-seq: read pairs 1501, FPKM 66.0, percentile rank 71.2% (100% = highest expression)

Protein function

Annotation:MET8Siroheme biosynthesis protein MET8
KEGG:K02304MET8 precorrin-2 dehydrogenase / sirohydrochlorin ferrochelatase [EC:1.3.1.76 4.99.1.4]
EGGNOG:0PFN2MET8Siroheme synthase
SGD closest match:S000000417MET8Siroheme biosynthesis protein MET8
CGD closest match:CAL0000198876MET8Bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase

Protein alignments

%idAln lengthE-value
MIA_02287_153.21%2651e-89MIA_02287_1
A0A0J9XH90_GEOCN43.43%2745e-72Similar to Saccharomyces cerevisiae YBR213W MET8 Bifunctional dehydrogenase and ferrochelatase OS=Geotrichum candidum GN=BN980_GECA16s02518g PE=4 SV=1
UniRef50_A0A0J9XH9043.43%2741e-68Similar to Saccharomyces cerevisiae YBR213W MET8 Bifunctional dehydrogenase and ferrochelatase n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9XH90_GEOCN
A0A167CD68_9ASCO37.27%2714e-50Bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase MET8 OS=Sugiyamaella lignohabitans GN=MET8 PE=4 SV=1
Q6C695_YARLI32.51%2834e-44YALI0E11385p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E11385g PE=4 SV=1
A0A1E3PKF5_9ASCO33.80%2841e-39Siroheme synthase middle domains-like protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_51189 PE=4 SV=1
Q5ADR1_CANAL30.74%2833e-33Bifunctional precorrin-2 dehydrogenase/sirohydrochlorin ferrochelatase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=MET8 PE=4 SV=1
A0A060T896_BLAAD30.47%2565e-32ARAD1D01892p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D01892g PE=4 SV=1
A0A1E4TGN5_9ASCO33.66%2057e-25Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_44538 PE=4 SV=1
MET8_YEAST24.18%2442e-10Siroheme biosynthesis protein MET8 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MET8 PE=1 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.1045

Protein family membership

None predicted.

Domains and repeats

  1. Domain
  2. Domain
1 50 100 150 200 250 280

Detailed signature matches

  1. Domain IPR016040
    NAD(P)-binding domain
    1. SSF51735 (NAD(P)-bi...)
  2. Domain IPR006367
    Sirohaem synthase, N-terminal
    1. PF13241 (NAD_binding_7)
    2. TIGR01470
  3. Domain IPR028281
    Siroheme synthase, central domain
    1. PF14824 (Sirohm_syn...)
  4. Domain IPR028162
    Siroheme biosynthesis protein Met8, C-terminal
    1. PF14823 (Sirohm_syn...)
Unintegrated signatures no IPR
Unintegrated signatures
  1. G3DSA:1.10.32...
  2. G3DSA:3.30.16...
  3. G3DSA:3.40.50...
  4. SSF75615 (Siroheme ...)
  5. mobidb-lite (disord...)

Protein sequence

>MCA_05823_1
MNIQPGGASILAWNIKNKPALVVGAGDVASGRVGHLLNSDAIVTVVAPSITNQDLKDFINSSSPLNTITYIAKSVSLDDH
DDDDKNDNNGSTDTNSNDKSISAHQLLIDPKTNEPKFEMVLTAIDTPGVSQAIHKVCKKYRIPVNVADVPPLCDFYFTST
IREGPLQVAVSTGGSAPRLARRVRQDIEKFVTSDLGIKAATFERTQKLRMKLREFTENHPELHETEKLKISSRMKWMSVV
CDTLPYKELAELTDEQIDEMVNRYPKRFRDEKDEEKTQEN

GO term prediction

Biological Process

GO:0006779 porphyrin-containing compound biosynthetic process
GO:0019354 siroheme biosynthetic process
GO:0055114 oxidation-reduction process

Molecular Function

GO:0043115 precorrin-2 dehydrogenase activity

Cellular Component

None predicted.