Protein

MCA_05572_1

Length
1,331 amino acids

Gene name: TOF1

Description: Topoisomerase 1-associated factor 1

Browser: contigD:1674975-1679038+

RNA-seq: read pairs 1139, FPKM 10.6, percentile rank 26.9% (100% = highest expression)

Protein function

Annotation:TOF1Topoisomerase 1-associated factor 1
KEGG:K10997TOF1 replication fork protection complex subunit Tof1/Swi1
EGGNOG:0PGK4TOF1Forms a fork protection complex (FPC) with csm-3 and which is required for chromosome segregation during meiosis and DNA damage repair. FPC coordinates leading and lagging strand synthesis and moves with the replication fork. FPC stabilizes replication forks in a configuration that is recognized by replication checkpoint sensors
SGD closest match:S000005217TOF1Topoisomerase 1-associated factor 1
CGD closest match:CAL0000201417YBL053Topoisomerase 1-associated factor 1

Protein alignments

%idAln lengthE-value
MIA_00887_131.26%10306e-118MIA_00887_1
A0A0J9X9A7_GEOCN29.12%10373e-115Similar to Saccharomyces cerevisiae YNL273W TOF1 Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion OS=Geotrichum candidum GN=BN980_GECA05s00879g PE=4 SV=1
UniRef50_A0A0J9X9A729.12%10376e-112Similar to Saccharomyces cerevisiae YNL273W TOF1 Subunit of a replication-pausing checkpoint complex (Tof1p-Mrc1p-Csm3p) that acts at the stalled replication fork to promote sister chromatid cohesion n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9X9A7_GEOCN
A0A1E3PNY7_9ASCO28.04%10201e-92Timeless-domain-containing protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_49586 PE=4 SV=1
A0A060TAB9_BLAAD28.51%8913e-91ARAD1C44572p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C44572g PE=4 SV=1
TOF1_YARLI26.82%8953e-72Topoisomerase 1-associated factor 1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=TOF1 PE=3 SV=1
TOF1_YEAST24.97%9212e-58Topoisomerase 1-associated factor 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TOF1 PE=1 SV=1
A0A167CHG9_9ASCO36.39%3492e-54Tof1p OS=Sugiyamaella lignohabitans GN=TOF1 PE=4 SV=1
TOF1_CANAL29.09%3852e-25Topoisomerase 1-associated factor 1 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=YBL053 PE=3 SV=2

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0478

Protein family membership

None predicted.

Domains and repeats

  1. Domain
1 200 400 600 800 1000 1200 1331

Detailed signature matches

  1. Domain IPR006906
    Timeless protein
    1. PF04821 (TIMELESS)
Unintegrated signatures no IPR
Unintegrated signatures
  1. Coil
  2. mobidb-lite (disord...)

Protein sequence

>MCA_05572_1
MSKPLHEESSTINVDTLSNSISNIVSALGGREITTTGSRYVLGDDALGCLQDLARWFKVINDDEKLMVSKAISTTTLGTI
DLVEILTQWQLEEEKLDKAQSLKEKKNKDKVNINNQEHNEEDVAKTKGQEALRAKKARIALACLELLVPMTWPLSFNTIS
SPTELLLMPEIRYSQAKYKAAILGHPKKRVLKAIIRIAMPLLETPKPNRTPRQESIINLVFLFFRNLLQINYRDNAATEF
GLDESSVLTDSISRNTEIRALYQQNVLDFLLVAASKCGDEFSSQTHIIMESLYYLLIGIRPIDIYPLDSAQKSHSLYKSL
SQFSQPESSLLSALKRDKAINSRIMAKQSSRHSNFGTLVAVKMPNGMSRPIASNLLNSKNVVELLYGQNPNSGTGITPQK
TTDGAFKINFDQLQDEKHLNSGFENGKSSGNRLKKQLEMLSSDTYEISVQLLSINQKVILRFLKKFFDISFNPMFKALRK
MFEQEFDSISLEYQSQYLRLVSFFLESQHLKLLTESQKNENSENNNEDEGDKWGFYAVAEALTENNFIIMRKIMDNAFEG
NHFAVLQSSMLCFQSLIRVVKEMASQGSSEVKELAENISLRLFREGRWLDVIAHIPRTANKQSWNYISTTVELSQLILTT
LQKYAFNNEGLYVRGKASKRPKKKAASKDSEKVSEDNKDQENESADEYETNQYVLKEQQFDFLKFEKKFYHEDTISTYIA
YLSRFQSMTESQLKLGFKLIMRFFSDNSMRVKFFRLDFFYLLYLMVDPQTYMDVSAAFRDFLSNFISYFMSQFALTCRST
PSILAEVPFEKQESYLPYLELGFEEYQLSQLTNEKEGKRKRSPPYAGKVASLLGFVEEQEFLLPYRNRLAVVVSAILDDG
QKPLLEWALKNLENAYTQREKWEESLADLSDSEQTSIAPQIAFTVSDKEEYEEYSPEEKGILVALRKNNKLRLLLEMTGC
QLLDSESKTLATLSSTAKLSELKESIMYIKQFLEDEVSLVMMFPEGKSAIDFLEVKRRKSQNKRKHPVTRKRANESGSED
DEEEEEEDRQFIDDDENSAYETDENGVQRKLQKSTHGRHKKRSKQKKRRRLQGSSTKSAPEKEQLVIKSSEFVSPSDDES
DEERDKMIYEREALQIQKNAELFKFPAQKSGDSVATNKKQIDLSIFDDDDENVDDNNEDVLEYSNADSDEDSSNNQDENS
NLDKDGNDDENVDANESENTNEDSNSKENGNAKANEKNNTSANSNKENSNKDASSKEQDKQNNNSKIASEEEKIKQAKIR
ELIEGLNRFAEITRRDMEEYDNYSDDDPGVAAELEMESILGIDSDYSDDYY

GO term prediction

Biological Process

None predicted.

Molecular Function

None predicted.

Cellular Component

None predicted.