Protein

MCA_05164_1

Length
1,122 amino acids


Gene name: MSH3

Description: DNA mismatch repair protein MSH3

Browser: contigD:505213-508582-

RNA-seq: read pairs 384, FPKM 4.2, percentile rank 16.2% (100% = highest expression)

Protein function

Annotation:MSH3DNA mismatch repair protein MSH3
KEGG:K08736MSH3 DNA mismatch repair protein MSH3
EGGNOG:0PGVCMSH3Component of the post-replicative DNA mismatch repair system (MMR). Heterodimerizes with msh-2 to form MutS beta, which binds to DNA mismatches thereby initiating DNA repair. Msh-3 provides substrate-binding and substrate-specificity to the complex. When bound, the MutS beta heterodimer bends the DNA helix and shields approximately 20 base pairs. Acts mainly to repair insertion-deletion loops (IDLs) from 2 to 13 nucleotides in size, but can also repair base-base and single insertion-deletion mismatches that occur during replication. After mismatch binding, forms a ternary complex with the MutL alpha heterodimer, which is thought to be responsible for directing the downstream MMR events, including strand discrimination, excision, and resynthesis. ATP binding and hydrolysis play a pivotal role in mismatch repair functions (By similarity)
SGD closest match:S000000688MSH3DNA mismatch repair protein MSH3
CGD closest match:CAL0000179035MSH3DNA mismatch repair protein MSH3

Protein alignments

%idAln lengthE-value
MIA_04715_142.09%11380.0MIA_04715_1
A0A1E3PGX6_9ASCO40.39%9210.0Uncharacterized protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_52294 PE=3 SV=1
UniRef50_A0A1E3PGX640.39%9210.0Uncharacterized protein n=1 Tax=Nadsonia fulvescens var. elongata DSM 6958 TaxID=857566 RepID=A0A1E3PGX6_9ASCO
A0A0J9X3S9_GEOCN38.33%10200.0Similar to Saccharomyces cerevisiae YCR092C MSH3 Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends OS=Geotrichum candidum GN=BN980_GECA02s01858g PE=3 SV=1
A0A167EK33_9ASCO40.24%9320.0Mismatch repair protein MSH3 OS=Sugiyamaella lignohabitans GN=MSH3 PE=3 SV=1
A0A060TCH1_BLAAD38.79%9720.0ARAD1D39248p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D39248g PE=3 SV=1
A0A1E4THF9_9ASCO33.11%9093e-162Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_31913 PE=3 SV=1
MSH3_YARLI33.48%9293e-144DNA mismatch repair protein MSH3 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=MSH3 PE=3 SV=1
MSH3_CANAL32.43%9624e-136DNA mismatch repair protein MSH3 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=MSH3 PE=3 SV=1
MSH3_YEAST32.38%9458e-135DNA mismatch repair protein MSH3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MSH3 PE=1 SV=2

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.6573

Protein family membership

Domains and repeats

Detailed signature matches

    1. PF01624 (MutS_I)
    1. SSF55271 (DNA repai...)
    1. SSF53150 (DNA repai...)
    2. PF05188 (MutS_II)
    1. SM00533 (DNAend)
    2. SSF48334 (DNA repai...)
    3. PF05192 (MutS_III)
    1. PF05190 (MutS_IV)
    1. SSF52540 (P-loop co...)
    1. PF00488 (MutS_V)
    2. SM00534 (mutATP5)
    3. PS00486 (DNA_MISMAT...)
Unintegrated signatures no IPR
Unintegrated signatures
  1. mobidb-lite (disord...)

Protein sequence

>MCA_05164_1
MSSSSSRRKPESSNKKFSKSSQHLFSKYFFSQKKDSEINEEQKKQISEVPVNSVGKRPLEETEIGKSNKKIKTLQNAAYP
KEKLKLPSLKQVEDVTKKRRLAFSFNPSSIKISDKPSSVIPSERHEDFVKKLEKVEKSKALQEKENSVEDDYSDGTNDVN
VKKGTKKLTPMNQQYVDLKKQHPDVLLLIEMGYKYQMFGSDAQTVSKELHTFIKPGKLTIDPNDPNDAKYDTYAYTTFLS
TALDKNVSKLVKLGYKVGIVEQMETSALKAQGSNKSAPFQRKLTRLYTKGTYIYNQTMESFQGKGLNSNRETVYSDGYLI
AICENKTFTDQFHIGFLAIQLSTGEVIIDQFQDTVMHTELETRLLHLSPLEILLVGKVNSNITKLIDQLTNSNTLNLEPR
IAKVDQVDESDVSMDLHNFYKNLLSEPSNEEFQKKTYQYLDYVDQLPNLVKICLVAVINYLKEFELETVFKHNQTFIPFS
SKSHMNLNGNTIVSLELFQNLFDHTSHGSLFSILDFTRTPFGQRLLKKWITKPLVDKNLIEERISVTQELAHNFTESMSF
LLNSMSKLPDLEKGLASIYYKRIKPRQLYYFLSNIKGICNIINNAPAETFQFTTKPLKNIVDCIKMAFDPCNKYLDELNT
DQVSEFDSDITCVFKNQLEKYDEISDFMIRITLTQEDIENLCFTTKSEHGFKTLSYVTINKSPYYFNVSTSETGKVPTDW
IKYSQTKTYGRYRSPDLEKKIRDLLFLQDSLKECCQKAYKSFLQEISDNHFETFKQTIQSLATLDCFLSLTAVSAQSGYV
KPQFVDAPCIKFEQGRHPVVERFKNSGGDTEKCENNDNNTNGAFRYVPNDTKMETDDNRVVILTGPNMGGKSSYARQVGL
ICIMAQIGSFVPCKSACLGIIDGIYTRMGAQDNFMRGESTFQVELKECRDILVSATDRSLVILDEIGRGTGTMDGVSIAY
AVLKSMIEDVKSFTLFITHYQNLTKVAAESTNNSLGKKMICNYHMGFKEHDLMEDNDTDKSNMGFQKTRIEFLYTLEPGI
AHRSYGLYVAQLANLSPEIIQRAYEISKHLETVINKRKLDKWTKIMCQFLLDYEKSNEKEKKEEFSIMEKNQLKYLFNSL
EN

GO term prediction

Biological Process

GO:0006298 mismatch repair

Molecular Function

GO:0005524 ATP binding
GO:0030983 mismatched DNA binding

Cellular Component

None predicted.