Protein

MCA_05106_1

Length
352 amino acids

Description: Putative uncharacterized oxidoreductase YDR541C

Browser: contigD:376505-377564-

RNA-seq: read pairs 2177, FPKM 76.2, percentile rank 74.5% (100% = highest expression)

Protein function

Annotation:Putative uncharacterized oxidoreductase YDR541C
EGGNOG:0PJT2GRE2NADPH-dependent methylglyoxal reductase GRE2
SGD closest match:S000002949YDR541CPutative uncharacterized oxidoreductase YDR541C
CGD closest match:CAL0000179051orf19.5611Methylglyoxal reductase (NADPH-dependent)

Protein alignments

%idAln lengthE-value
MIA_04745_159.94%3422e-148MIA_04745_1
A0A0J9XCC0_GEOCN53.87%3361e-130Similar to Saccharomyces cerevisiae YGL157W ARI1 NADPH-dependent aldehyde reductase OS=Geotrichum candidum GN=BN980_GECA07s05433g PE=4 SV=1
UniRef50_A0A0J9XB2252.23%3379e-121Similar to Saccharomyces cerevisiae YGL157W ARI1 NADPH-dependent aldehyde reductase n=2 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9XB22_GEOCN
Q5ABT9_CANAL41.84%3371e-86Methylglyoxal reductase (NADPH-dependent) OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=orf19.5611 PE=4 SV=1
A0A060T4V0_BLAAD42.23%3413e-84ARAD1B02860p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1B02860g PE=4 SV=1
YD541_YEAST44.15%3425e-84Putative uncharacterized oxidoreductase YDR541C OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=YDR541C PE=3 SV=2
A0A167C177_9ASCO41.64%3412e-83Carbonyl reductase (NADPH-dependent) OS=Sugiyamaella lignohabitans GN=AWJ20_3889 PE=4 SV=1
A0A1E4T9D1_9ASCO43.49%3389e-83Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_4495 PE=4 SV=1
A0A1E3PSR4_9ASCO41.95%3489e-81Putative NADPH-dependent methylglyoxal reductase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_45022 PE=4 SV=1
Q6C9S0_YARLI42.55%3297e-81YALI0D08844p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_D08844g PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.9507
Predicted cleavage: 50

Protein family membership

None predicted.

Domains and repeats

  1. Domain
1 50 100 150 200 250 300 352

Detailed signature matches

  1. Domain IPR016040
    NAD(P)-binding domain
    1. SSF51735 (NAD(P)-bi...)
  2. Domain IPR002225
    3-beta hydroxysteroid dehydrogenase/isomerase
    1. PF01073 (3Beta_HSD)
Unintegrated signatures no IPR
Unintegrated signatures
  1. G3DSA:3.40.50...
  2. G3DSA:3.90.25.10
  3. NON_CYTOPLASM... (N...)
  4. SIGNAL_PEPTIDE (Sig...)
  5. SIGNAL_PEPTID... (S...)
  6. SIGNAL_PEPTID... (S...)
  7. SIGNAL_PEPTID... (S...)
  8. cd05227 (AR_SDR_e)

Residue annotation

  1. NADP binding site ...
  2. putative substrate...
  3. active site cd05227

Protein sequence

>MCA_05106_1
MSTKTTTHKKVLLTGASGFIAIHTLQTLLSRGYQVKATVRSIPKGDYLVSKFPNLPLEIAIVPDISEPNAFDTVLKEDSE
ITAVIHMASPFFHPQSDPETQILKPAIQGTRNIVQAVHKFAPQVTKVVITSSFASILDVDKMNDHSTVFSEESWSPITWQ
ESVEDLSKTYRGSKKLAEKEFWKFINENDDVNFSGTTVNPPFVFGPLIQQVSDIKSINTSNAVIYNTLFGENSKEDKDYT
ELTHLWVDVRDVALAHVLPLEDEESKFNGQRLFTTAGYFSPQLILDIANEKFPELKGKIPVGKPGTGLDRVDKIYQYDNK
KTNELLNFEYRKFEDTIVETIQSILEFKEILN

GO term prediction

Biological Process

GO:0006694 steroid biosynthetic process
GO:0055114 oxidation-reduction process

Molecular Function

GO:0003854 3-beta-hydroxy-delta5-steroid dehydrogenase activity
GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Cellular Component

None predicted.