Protein

MCA_04741_1

Length
366 amino acids

Gene name: LEU2

Description: 3-isopropylmalate dehydrogenase

Browser: contigC:3885546-3886647-

RNA-seq: read pairs 2738, FPKM 92.2, percentile rank 77.3% (100% = highest expression)

Protein function

Annotation:LEU23-isopropylmalate dehydrogenase
KEGG:K00052leuB 3-isopropylmalate dehydrogenase [EC:1.1.1.85]
EGGNOG:0PFHGLEU2Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate
SGD closest match:S000000523LEU23-isopropylmalate dehydrogenase
CGD closest match:CAL0000185901LEU23-isopropylmalate dehydrogenase

Protein alignments

%idAln lengthE-value
MIA_05247_191.39%3600.0MIA_05247_1
A0A0J9XAW1_GEOCN89.14%3590.03-isopropylmalate dehydrogenase OS=Geotrichum candidum GN=BN980_GECA07s02881g PE=3 SV=1
LEU3_BLAAD81.16%3610.03-isopropylmalate dehydrogenase OS=Blastobotrys adeninivorans GN=LEU2 PE=3 SV=1
UniRef50_Q96WT980.72%3630.03-isopropylmalate dehydrogenase n=31 Tax=saccharomyceta TaxID=716545 RepID=LEU3_SACEX
LEU3_YEAST79.45%3650.03-isopropylmalate dehydrogenase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=LEU2 PE=1 SV=4
A0A167FCG6_9ASCO80.06%3610.03-isopropylmalate dehydrogenase OS=Sugiyamaella lignohabitans GN=LEU2 PE=3 SV=1
A0A1D8PQK5_CANAL75.48%3670.03-isopropylmalate dehydrogenase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=LEU2 PE=3 SV=1
A0A1E3PPA0_9ASCO74.17%3600.03-isopropylmalate dehydrogenase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_81723 PE=3 SV=1
A0A1E4TM50_9ASCO68.32%3631e-1723-isopropylmalate dehydrogenase OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_93497 PE=3 SV=1
LEU3_YARLI68.08%3547e-1593-isopropylmalate dehydrogenase OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=LEU2 PE=3 SV=2

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0151

Protein family membership

Domains and repeats

  1. Domain
1 50 100 150 200 250 300 366

Detailed signature matches

  1. Family IPR004429
    Isopropylmalate dehydrogenase
    1. TIGR00169
  2. Domain IPR024084
    Isopropylmalate dehydrogenase-like domain
    1. SM01329 (Iso_dh_2)
    2. PF00180 (Iso_dh)
  3. SITE IPR019818
    Isocitrate/isopropylmalate dehydrogenase, conserved site
    1. PS00470 (IDH_IMDH)
Unintegrated signatures no IPR
Unintegrated signatures
  1. G3DSA:3.40.71...
  2. SSF53659 (Isocitrat...)

Protein sequence

>MCA_04741_1
MSAPKTKTVVVLGGDHVGPEVVAEAIKVMKAIEEVRPNIKFDFQQHLIGGAAIDATGNPLPDETLEAAKKADAVLLGAVG
GPKWGTGAVRPEQGLLKIRKELNLYANLRPCNFISEALYDLSPLKPEVVKGTDFTIVRELVGGIYFGEREEDNGTGKAWD
TEAYSVAEVQRLTRMAAFLALQSNPPLPVWSLDKANVLASSRLWRKTVTETIEKEFPQLTLNHQLIDSAAMIMVKNPSSL
NGVLITTNMFGDILSDEASVIPGSLGLLPSASLASLPDKNTAFGLYEPCHGTAPDLPKNKVNPVATILSTAMMLKLSLDM
KEEGEALEKAVRSVLDSGIRTGDLKGTNSTTEVGDAIAAEVKKLLQ

GO term prediction

Biological Process

GO:0009098 leucine biosynthetic process
GO:0055114 oxidation-reduction process

Molecular Function

GO:0000287 magnesium ion binding
GO:0003862 3-isopropylmalate dehydrogenase activity
GO:0016616 oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor
GO:0051287 NAD binding

Cellular Component

GO:0005737 cytoplasm