MCA_04721_1
Gene name: ISM1
Description: Isoleucine--tRNA ligase, mitochondrial
Browser: contigC:3812357-3815601+
RNA-seq: read pairs 1773, FPKM 21.8, percentile rank 43.6% (100% = highest expression)
Protein function
| Annotation: | ISM1 | Isoleucine--tRNA ligase, mitochondrial | |
|---|---|---|---|
| KEGG: | K01870 | IARS | isoleucyl-tRNA synthetase [EC:6.1.1.5] |
| EGGNOG: | 0PGFU | ISM1 | Isoleucyl-tRNA synthetase |
| SGD closest match: | S000005961 | ISM1 | Isoleucine--tRNA ligase, mitochondrial |
| CGD closest match: | CAL0000185259 | CAALFM_CR03400WA | Isoleucine--tRNA ligase |
Protein alignments
| %id | Aln length | E-value | ||
|---|---|---|---|---|
| MIA_05255_1 | 65.99% | 985 | 0.0 | MIA_05255_1 |
| A0A0J9X6B1_GEOCN | 62.54% | 969 | 0.0 | Similar to Saccharomyces cerevisiae YPL040C ISM1 Mitochondrial isoleucyl-tRNA synthetase OS=Geotrichum candidum GN=BN980_GECA03s06104g PE=3 SV=1 |
| UniRef50_A0A0J9X6B1 | 62.54% | 969 | 0.0 | Similar to Saccharomyces cerevisiae YPL040C ISM1 Mitochondrial isoleucyl-tRNA synthetase n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9X6B1_GEOCN |
| A0A1E3PK96_9ASCO | 49.59% | 980 | 0.0 | Isoleucyl-tRNA synthetase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_51139 PE=3 SV=1 |
| Q6CCB9_YARLI | 50.15% | 971 | 0.0 | YALI0C10780p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_C10780g PE=3 SV=1 |
| A0A060TE49_BLAAD | 45.53% | 973 | 0.0 | ARAD1D49060p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D49060g PE=3 SV=1 |
| A0A1E4TL64_9ASCO | 45.53% | 951 | 0.0 | Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_56159 PE=3 SV=1 |
| A0A1D8PSG5_CANAL | 43.09% | 998 | 0.0 | Isoleucine--tRNA ligase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=CAALFM_CR03400WA PE=3 SV=1 |
| SYIM_YEAST | 42.38% | 1010 | 0.0 | Isoleucine--tRNA ligase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=ISM1 PE=1 SV=2 |
| A0A167EHT4_9ASCO | 48.24% | 568 | 2e-164 | Isoleucine--tRNA ligase ISM1 OS=Sugiyamaella lignohabitans GN=ISM1 PE=3 SV=1 |
Mitochondrial localization by mitoprotII
Probability of mitochondrial location: 0.9290
Predicted cleavage: 30
Protein family membership
- Isoleucine-tRNA ligase (IPR002301)
- Isoleucine-tRNA ligase, type 1 (IPR023585)
Domains and repeats
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Domain
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Domain
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Domain
Detailed signature matches
no IPR
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SSF52374 (Nucleotid...)
Residue annotation
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tRNA binding surfa...
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anticodon binding ...
Protein sequence
>MCA_04721_1 MIRASVGKRTLVLKSQLNSCFNSGVIRLYATAQKPDDSKKPSSHGFSDTVILPKTDFPNRSGSQETIQKLIKRSSDDLYQ WQYKSDKFQEKSVFHDGPPYANAKLHLGHAMNKILKDIINRFELMKGKKVEYIPGWDCHGLPIELKALQTLSEKAKTDVS TVLSPTEIRTIARQHALQAIDDQKKSFKSFGVMGDWDNSYRTLDPDYEVRQLNVFKEMLHRGYISRKIKPVYWGTETRTA LAEAELEYNDSHKSTAVYVKFPLYYIDDRLKKILNGQIPSNKTLHALIWTTTPWTLVANRAIAVNPDLHYVVVKHKKHGY LIVAEARLEELGEEYEIVESIKSFRGKVLTSSTYKNPLLNFMTAKDQPILAAEYVTSTAGTGLVHTAPGHGFDDYQLCSK HKIDSYSPVDDEGKYTKDVPEELEQISGLFVLNKGQKEVIKILENNNALVSINRKYIHKYPYDWRSKLPIIIRSTPQWFA DVDAVKKNAIKSLQKVHFHPSNGRNRLEAFTESRSEWCISRQRVWGVPIPAIYNKETGEPLTDVATMDHIISKIAEFGTN AWFEEEDNVQRWLPKTKEFQGKGAKYFKGKDTMDVWFDSGTSWTMLENREKDGKYLADYYLEGSDQHRGWFQSSLLTKMS VSKKAKAPFRNIITHGFTLDEKGQKMSKSLGNTISPETVMKGKKGQWLPLGVDGLRLWVASSDYTKDVSVGPTILKNTAE NLKKLRFTLRFLLGNLSSYNPKEFGDFDPKKFHPVDLYILHLLKKMADTAKEHYESFAFNRVMQTVNYHTSTELSAFYFD IIKDRLYAAPEKSVDRQAALFVLNEILKVYMSVLSPITPLLTQEAWDHSSPHIQNGLDSPFKAGWYELPEVYENEEIAKE FSKIEDVIKIVNQAMESARVAKSMTSSLSCDVNLHFKEGNEIADLLKKYEPYLAELLICSQVNINPTESSSNGAWVYESS STIDGVEVRAETIPTQHHKCPRCWQYTAEEEDKLCHRCEDAVSQL
GO term prediction
Biological Process
GO:0006418 tRNA aminoacylation for protein translation
GO:0006428 isoleucyl-tRNA aminoacylation
Molecular Function
GO:0000049 tRNA binding
GO:0000166 nucleotide binding
GO:0002161 aminoacyl-tRNA editing activity
GO:0004812 aminoacyl-tRNA ligase activity
GO:0004822 isoleucine-tRNA ligase activity
GO:0005524 ATP binding
Cellular Component
GO:0005737 cytoplasm