Protein

MCA_04601_1

Length
620 amino acids

Gene name: TRM2

Description: tRNA (uracil(54)-C(5))-methyltransferase

Browser: contigC:3480393-3482256-

RNA-seq: read pairs 1222, FPKM 24.3, percentile rank 46.9% (100% = highest expression)

Protein function

Annotation:TRM2tRNA (uracil(54)-C(5))-methyltransferase
KEGG:K15331TRMT2B tRNA (uracil-5-)-methyltransferase [EC:2.1.1.35]
EGGNOG:0PGD0TRM2trna (uracil-5-)-methyltransferase
SGD closest match:S000001764TRM2tRNA (uracil(54)-C(5))-methyltransferase
CGD closest match:CAL0000192336TRM2Trm2p

Protein alignments

%idAln lengthE-value
MIA_01763_168.14%4990.0MIA_01763_1
A0A0J9X8C1_GEOCN61.43%5160.0Similar to Saccharomyces cerevisiae YKR056W TRM2 tRNA methyltransferase OS=Geotrichum candidum GN=BN980_GECA05s01748g PE=3 SV=1
A0A060TCV7_BLAAD58.56%4850.0ARAD1D38940p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D38940g PE=3 SV=1
A0A161HLU2_9ASCO57.14%5110.0Trm2p OS=Sugiyamaella lignohabitans GN=TRM2 PE=3 SV=1
UniRef50_A0A161HLU257.14%5110.0Trm2p n=3 Tax=Saccharomycetales TaxID=4892 RepID=A0A161HLU2_9ASCO
Q6C110_YARLI47.14%5071e-150YALI0F20196p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_F20196g PE=3 SV=1
A0A1D8PCG3_CANAL44.97%5272e-143Trm2p OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=TRM2 PE=3 SV=1
TRM2_YEAST44.77%5451e-138tRNA (uracil(54)-C(5))-methyltransferase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TRM2 PE=1 SV=2
A0A1E3PND4_9ASCO52.25%4004e-138S-adenosyl-L-methionine-dependent methyltransferase (Fragment) OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_82207 PE=3 SV=1
A0A1E4TAD2_9ASCO44.65%4951e-121Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_32229 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.9839

Protein family membership

Domains and repeats

  1. Domain
1 100 200 300 400 500 620

Detailed signature matches

  1. Family IPR010280
    (Uracil-5)-methyltransferase family
    1. PF05958 (tRNA_U5-me...)
    2. PS51687 (SAM_MT_RNA...)
  2. Family IPR025795
    tRNA (uracil-5-)-methyltransferase
    1. PS51622 (SAM_MT_RNA...)
  3. Domain IPR002792
    TRAM domain
    1. PF01938 (TRAM)
    2. PS50926 (TRAM)
  4. Domain IPR029063
    S-adenosyl-L-methionine-dependent methyltransferase
    1. SSF53335 (S-adenosy...)
  5. SITE IPR030390
    RNA methyltransferase TrmA, active site
    1. PS01230 (TRMA_1)
  6. SITE IPR030391
    RNA methyltransferase TrmA, conserved site
    1. PS01231 (TRMA_2)
Unintegrated signatures no IPR
Unintegrated signatures
  1. G3DSA:2.40.50...
  2. G3DSA:3.40.50...
  3. cd02440 (AdoMet_MTases)
  4. mobidb-lite (disord...)

Residue annotation

  1. S-adenosylmethioni...

Protein sequence

>MCA_04601_1
MRFRSWKLIFNRVNFHNHITRPQYNILHHNLKLNKFYSSTMSDPTVAIEPMAQVPVESDKTSQVSQMIPIKRALSPSASG
EPTNTKIAKEDTENNDNNSNNNNNNNNGNNRNRQKKKKTGPKPRRFKEPPVADPVSPEGVLITDIKTFLASKEITEQQVI
NNLREFFTRPRDFVHPEHRVVTVTIEARSGSGEGIGLVKLENSNVKEGESTTEQKQEYQVVVIPFTVIGDVVEAKIYKTQ
RYYFNAELLKVITPSPQRDDSLIKCKYFGKCSGCQYQMISYDDQLEIKRNVIVNAYKHNSDLDSALIPDILPTFPSPKQF
YYRTKLTPHFDINKKTVSCPPVGFAQPGRKAIIDIEECPIGTPVINQGLTKQREWLKENYTSYKRAATFLLRENTIEKPK
EDDPSVVEKTTICATSTKDIITEYVGDKVFKFPASSFFQNNNSILVDVTNYVKENIVLPKTGLPPTYLVDAYCGCGLFSI
TCCGSAKSVIGVEISQDSVRYAQKNAELNNVDAQFVVGQAERIFENVDTDPQETSIIIDPPRKGCDELFLNQLLKFKPAK
IVYISCNVHSQARDIGYLLKNNKEEDSSHYVIESIRGFDFFPQTHHVESVAVLSLKDKNS

GO term prediction

Biological Process

GO:0006396 RNA processing
GO:0008033 tRNA processing

Molecular Function

GO:0008173 RNA methyltransferase activity
GO:0030697 S-adenosylmethionine-dependent tRNA (m5U54) methyltransferase activity

Cellular Component

None predicted.