MCA_04524_1

Protein

Length

310 amino acids

RNA-seq: read pairs 563, FPKM 22.4, percentile rank 44.4% (100% = highest expression)

Protein function

KEGG:	K14407	CSTF2	cleavage stimulation factor subunit 2
EGGNOG:	0PMBW	FG00370.1	polyadenylation factor subunit CstF64
SGD closest match:	S000003012	RNA15	mRNA 3'-end-processing protein RNA15
CGD closest match:	CAL0000175047	orf19.4538	Uncharacterized protein

Protein alignments

	%id	Aln length	E-value
MIA_03404_1	73.94%	188	2e-84	MIA_03404_1
A0A0J9XHS0_GEOCN	62.43%	181	9e-67	Similar to Saccharomyces cerevisiae YGL044C RNA15 Component of the cleavage and polyadenylation factor I (CF I) OS=Geotrichum candidum GN=BN980_GECA17s01374g PE=4 SV=1
UniRef50_A0A0J9XHS0	62.43%	181	2e-63	Similar to Saccharomyces cerevisiae YGL044C RNA15 Component of the cleavage and polyadenylation factor I (CF I) n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9XHS0_GEOCN
A0A1E3PFZ8_9ASCO	51.81%	193	2e-59	Uncharacterized protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_71470 PE=4 SV=1
A0A167CJL6_9ASCO	58.89%	180	1e-57	Rna15p OS=Sugiyamaella lignohabitans GN=RNA15 PE=4 SV=1
A0A060TI19_BLAAD	50.00%	194	7e-55	ARAD1D42834p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D42834g PE=4 SV=1
Q6C6I9_YARLI	43.48%	184	5e-45	YALI0E09174p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E09174g PE=4 SV=1
RNA15_YEAST	59.49%	79	2e-29	mRNA 3'-end-processing protein RNA15 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RNA15 PE=1 SV=1
A0A1E4TFM7_9ASCO	28.89%	315	9e-29	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_2290 PE=4 SV=1
Q59T93_CANAL	40.24%	164	6e-22	Uncharacterized protein OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=orf19.4538 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0042

Protein family membership

None predicted.

Domains and repeats

Domain
3 - 92
RNA recognition motif domain (IPR000504)
105 - 184
Cleavage stimulation factor subunit 2, hinge domain (IPR025742)
269 - 305
Transcription termination and cleavage factor, C-terminal domain (IPR026896)

1 50 100 150 200 250 310

Detailed signature matches

IPR000504

RNA recognition motif domain
1. SSF54928 (RNA-bindi...)
  
  3 - 92
  
  SUPERFAMILY
  SSF54928 (RNA-binding domain, RBD)
2. PS50102 (RRM)
  
  7 - 85
  
  PROSITE profiles
  PS50102 (RRM)
3. SM00360 (rrm1_1)
  
  8 - 81
  
  SMART
  SM00360 (rrm1_1)
4. PF00076 (RRM_1)
  
  9 - 79
  
  Pfam
  PF00076 (RRM_1)
IPR025742

Cleavage stimulation factor subunit 2, hinge domain
1. PF14327 (CSTF2_hinge)
  
  105 - 184
  
  Pfam
  PF14327 (CSTF2_hinge)
IPR026896

Transcription termination and cleavage factor, C-terminal domain
1. PF14304 (CSTF_C)
  
  269 - 305
  
  Pfam
  PF14304 (CSTF_C)

no IPR

Unintegrated signatures

G3DSA:3.30.70...

2 - 223

GENE3D
G3DSA:3.30.70.330 (G3DSA:3.30.70.330)
cd12398 (RRM_CSTF2_...)

9 - 83

CDD
cd12398 (RRM_CSTF2_RNA15_like)
mobidb-lite (disord...)

191 - 267

MobiDB Lite
mobidb-lite (disorder_prediction)

86 - 119

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

RNA binding site c...

10Y

RNA binding site
10Y, 37R, 38L, 39V, 40F, 41D, 42K, 43D, 44S, 47S, 50F, 51G, 52F, 76R, 79R, 80V, 81D, 82F, 83S
CDD
cd12398 (RRM_CSTF2_RNA15_like)

37R

RNA binding site
10Y, 37R, 38L, 39V, 40F, 41D, 42K, 43D, 44S, 47S, 50F, 51G, 52F, 76R, 79R, 80V, 81D, 82F, 83S
CDD
cd12398 (RRM_CSTF2_RNA15_like)

38L

RNA binding site
10Y, 37R, 38L, 39V, 40F, 41D, 42K, 43D, 44S, 47S, 50F, 51G, 52F, 76R, 79R, 80V, 81D, 82F, 83S
CDD
cd12398 (RRM_CSTF2_RNA15_like)

39V

RNA binding site
10Y, 37R, 38L, 39V, 40F, 41D, 42K, 43D, 44S, 47S, 50F, 51G, 52F, 76R, 79R, 80V, 81D, 82F, 83S
CDD
cd12398 (RRM_CSTF2_RNA15_like)

40F

RNA binding site
10Y, 37R, 38L, 39V, 40F, 41D, 42K, 43D, 44S, 47S, 50F, 51G, 52F, 76R, 79R, 80V, 81D, 82F, 83S
CDD
cd12398 (RRM_CSTF2_RNA15_like)

41D

RNA binding site
10Y, 37R, 38L, 39V, 40F, 41D, 42K, 43D, 44S, 47S, 50F, 51G, 52F, 76R, 79R, 80V, 81D, 82F, 83S
CDD
cd12398 (RRM_CSTF2_RNA15_like)

42K

RNA binding site
10Y, 37R, 38L, 39V, 40F, 41D, 42K, 43D, 44S, 47S, 50F, 51G, 52F, 76R, 79R, 80V, 81D, 82F, 83S
CDD
cd12398 (RRM_CSTF2_RNA15_like)

43D

RNA binding site
10Y, 37R, 38L, 39V, 40F, 41D, 42K, 43D, 44S, 47S, 50F, 51G, 52F, 76R, 79R, 80V, 81D, 82F, 83S
CDD
cd12398 (RRM_CSTF2_RNA15_like)

44S

RNA binding site
10Y, 37R, 38L, 39V, 40F, 41D, 42K, 43D, 44S, 47S, 50F, 51G, 52F, 76R, 79R, 80V, 81D, 82F, 83S
CDD
cd12398 (RRM_CSTF2_RNA15_like)

47S

RNA binding site
10Y, 37R, 38L, 39V, 40F, 41D, 42K, 43D, 44S, 47S, 50F, 51G, 52F, 76R, 79R, 80V, 81D, 82F, 83S
CDD
cd12398 (RRM_CSTF2_RNA15_like)

50F

RNA binding site
10Y, 37R, 38L, 39V, 40F, 41D, 42K, 43D, 44S, 47S, 50F, 51G, 52F, 76R, 79R, 80V, 81D, 82F, 83S
CDD
cd12398 (RRM_CSTF2_RNA15_like)

51G

RNA binding site
10Y, 37R, 38L, 39V, 40F, 41D, 42K, 43D, 44S, 47S, 50F, 51G, 52F, 76R, 79R, 80V, 81D, 82F, 83S
CDD
cd12398 (RRM_CSTF2_RNA15_like)

52F

RNA binding site
10Y, 37R, 38L, 39V, 40F, 41D, 42K, 43D, 44S, 47S, 50F, 51G, 52F, 76R, 79R, 80V, 81D, 82F, 83S
CDD
cd12398 (RRM_CSTF2_RNA15_like)

76R

RNA binding site
10Y, 37R, 38L, 39V, 40F, 41D, 42K, 43D, 44S, 47S, 50F, 51G, 52F, 76R, 79R, 80V, 81D, 82F, 83S
CDD
cd12398 (RRM_CSTF2_RNA15_like)

79R

RNA binding site
10Y, 37R, 38L, 39V, 40F, 41D, 42K, 43D, 44S, 47S, 50F, 51G, 52F, 76R, 79R, 80V, 81D, 82F, 83S
CDD
cd12398 (RRM_CSTF2_RNA15_like)

80V

RNA binding site
10Y, 37R, 38L, 39V, 40F, 41D, 42K, 43D, 44S, 47S, 50F, 51G, 52F, 76R, 79R, 80V, 81D, 82F, 83S
CDD
cd12398 (RRM_CSTF2_RNA15_like)

81D

RNA binding site
10Y, 37R, 38L, 39V, 40F, 41D, 42K, 43D, 44S, 47S, 50F, 51G, 52F, 76R, 79R, 80V, 81D, 82F, 83S
CDD
cd12398 (RRM_CSTF2_RNA15_like)

82F

RNA binding site
10Y, 37R, 38L, 39V, 40F, 41D, 42K, 43D, 44S, 47S, 50F, 51G, 52F, 76R, 79R, 80V, 81D, 82F, 83S
CDD
cd12398 (RRM_CSTF2_RNA15_like)

83S

RNA binding site
10Y, 37R, 38L, 39V, 40F, 41D, 42K, 43D, 44S, 47S, 50F, 51G, 52F, 76R, 79R, 80V, 81D, 82F, 83S
CDD
cd12398 (RRM_CSTF2_RNA15_like)

Protein sequence

>MCA_04524_1
MEPIPSKVIYVGSIPYDQTEEQILDIFRSVGPVANFRLVFDKDSGKSKGFGFVEYHDPETAASAVRNLNNYSIGSRNLRV
DFSHETSITSPSSFPSSNDSTDLPPLPSGTMPPPGVPAHEAIGKTLMTLTRDQMLDIILDMKTMSQNNPSLAQSLLKSSP
QLSYMIVQSMIMMDIIDNNAVISLVGQDLQQPQPYQQQQPPRNTQQYNNNSGSEQQENKGNTTPVLNSSSQVNSPNNFQS
FSNSNTESNNAVSWPQSQQQASDGSDLNQQATIIRQIMQLTDEQVALLPDDQRQVLEVLRKRVQNGEISL

GO term prediction

Biological Process

GO:0031124 mRNA 3'-end processing

Molecular Function

GO:0003676 nucleic acid binding

Cellular Component