MCA_04503_1

Protein

Length

1,323 amino acids

Gene name: RAD50

Description: DNA repair protein RAD50

Browser: contigC:3198196-3202418-

RNA-seq: read pairs 1161, FPKM 10.8, percentile rank 27.3% (100% = highest expression)

Protein function

Annotation:		RAD50	DNA repair protein RAD50
KEGG:	K10866	RAD50	DNA repair protein RAD50 [EC:3.6.-.-]
EGGNOG:	0PG7Q	RAD50	DNA repair protein Rad50
SGD closest match:	S000005194	RAD50	DNA repair protein RAD50
CGD closest match:	CAL0000184671	RAD50	MRX complex DNA-binding subunit

Protein alignments

	%id	Aln length	E-value
MIA_01100_1	47.82%	1282	0.0	MIA_01100_1
A0A0J9X3K7_GEOCN	44.26%	1333	0.0	Similar to Saccharomyces cerevisiae YNL250W RAD50 Subunit of MRX complex, with Mre11p and Xrs2p OS=Geotrichum candidum GN=BN980_GECA02s00626g PE=4 SV=1
UniRef50_A0A0J9X3K7	44.26%	1333	0.0	Similar to Saccharomyces cerevisiae YNL250W RAD50 Subunit of MRX complex, with Mre11p and Xrs2p n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9X3K7_GEOCN
A0A060TDF0_BLAAD	40.52%	1335	0.0	ARAD1D47388p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D47388g PE=4 SV=1
Q6C910_YARLI	38.67%	1337	0.0	YALI0D15246p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_D15246g PE=4 SV=1
RAD50_YEAST	36.97%	1366	0.0	DNA repair protein RAD50 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RAD50 PE=1 SV=1
A0A1D8PJD1_CANAL	35.40%	1353	0.0	MRX complex DNA-binding subunit OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=RAD50 PE=4 SV=1
A0A167C6E1_9ASCO	35.49%	1206	0.0	MRX complex DNA-binding subunit OS=Sugiyamaella lignohabitans GN=RAD50 PE=4 SV=1
A0A1E3PR87_9ASCO	28.28%	343	8e-21	Uncharacterized protein (Fragment) OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_81044 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.1472
Predicted cleavage: 29

Protein family membership

DNA repair protein Rad50, eukaryotes (IPR004584)

Domains and repeats

Domain
28 - 107
P-loop containing nucleoside triphosphate hydrolase (IPR027417)
134 - 196
P-loop containing nucleoside triphosphate hydrolase (IPR027417)
860 - 898
P-loop containing nucleoside triphosphate hydrolase (IPR027417)
1,087 - 1,291
P-loop containing nucleoside triphosphate hydrolase (IPR027417)

1 200 400 600 800 1000 1200 1323

Detailed signature matches

IPR004584

DNA repair protein Rad50, eukaryotes
1. TIGR00606
  
  9 - 1,314
  
  TIGRFAMs
  TIGR00606 (TIGR00606)
IPR027417

P-loop containing nucleoside triphosphate hydrolase
1. SSF52540 (P-loop co...)
  
  1,087 - 1,291
  
  SUPERFAMILY
  SSF52540 (P-loop containing nucleoside triphosphate hydrolases)
  
  860 - 898
  
  SUPERFAMILY
  SSF52540 (P-loop containing nucleoside triphosphate hydrolases)
  
  28 - 107
  
  SUPERFAMILY
  SSF52540 (P-loop containing nucleoside triphosphate hydrolases)
  
  134 - 196
  
  SUPERFAMILY
  SSF52540 (P-loop containing nucleoside triphosphate hydrolases)
  
  1,210 - 1,290
  
  SUPERFAMILY
  SSF52540 (P-loop containing nucleoside triphosphate hydrolases)

no IPR

Unintegrated signatures

Coil

217 - 265

COILS
Coil (Coil)

516 - 543

COILS
Coil (Coil)

621 - 648

COILS
Coil (Coil)

304 - 324

COILS
Coil (Coil)

851 - 951

COILS
Coil (Coil)

772 - 840

COILS
Coil (Coil)

999 - 1,040

COILS
Coil (Coil)

357 - 394

COILS
Coil (Coil)

1,322 - 1,323

COILS
Coil (Coil)

720 - 750

COILS
Coil (Coil)

1,094 - 1,128

COILS
Coil (Coil)

276 - 296

COILS
Coil (Coil)

1,043 - 1,082

COILS
Coil (Coil)
G3DSA:1.10.28...

193 - 339

GENE3D
G3DSA:1.10.287.620 (G3DSA:1.10.287.620)
G3DSA:1.20.11...

515 - 840

GENE3D
G3DSA:1.20.1170.10 (G3DSA:1.20.1170.10)
G3DSA:1.20.12...

968 - 1,153

GENE3D
G3DSA:1.20.120.330 (G3DSA:1.20.120.330)
G3DSA:3.40.50...

1,154 - 1,310

GENE3D
G3DSA:3.40.50.300 (G3DSA:3.40.50.300)

9 - 192

GENE3D
G3DSA:3.40.50.300 (G3DSA:3.40.50.300)
PF13476 (AAA_23)

12 - 276

Pfam
PF13476 (AAA_23)
cd03240 (ABC_Rad50)

1,209 - 1,311

CDD
cd03240 (ABC_Rad50)

10 - 173

CDD
cd03240 (ABC_Rad50)
mobidb-lite (disord...)

336 - 357

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

Walker A/P-loop cd...

40G

Walker A/P-loop
40G, 41Q, 42N, 43G, 44S, 45G, 46K, 47T
CDD
cd03240 (ABC_Rad50)

41Q

Walker A/P-loop
40G, 41Q, 42N, 43G, 44S, 45G, 46K, 47T
CDD
cd03240 (ABC_Rad50)

42N

Walker A/P-loop
40G, 41Q, 42N, 43G, 44S, 45G, 46K, 47T
CDD
cd03240 (ABC_Rad50)

43G

Walker A/P-loop
40G, 41Q, 42N, 43G, 44S, 45G, 46K, 47T
CDD
cd03240 (ABC_Rad50)

44S

Walker A/P-loop
40G, 41Q, 42N, 43G, 44S, 45G, 46K, 47T
CDD
cd03240 (ABC_Rad50)

45G

Walker A/P-loop
40G, 41Q, 42N, 43G, 44S, 45G, 46K, 47T
CDD
cd03240 (ABC_Rad50)

46K

Walker A/P-loop
40G, 41Q, 42N, 43G, 44S, 45G, 46K, 47T
CDD
cd03240 (ABC_Rad50)

47T

Walker A/P-loop
40G, 41Q, 42N, 43G, 44S, 45G, 46K, 47T
CDD
cd03240 (ABC_Rad50)
ATP binding site c...

43G

ATP binding site
43G, 44S, 46K, 47T, 48T, 162H
CDD
cd03240 (ABC_Rad50)

44S

ATP binding site
43G, 44S, 46K, 47T, 48T, 162H
CDD
cd03240 (ABC_Rad50)

46K

ATP binding site
43G, 44S, 46K, 47T, 48T, 162H
CDD
cd03240 (ABC_Rad50)

47T

ATP binding site
43G, 44S, 46K, 47T, 48T, 162H
CDD
cd03240 (ABC_Rad50)

48T

ATP binding site
43G, 44S, 46K, 47T, 48T, 162H
CDD
cd03240 (ABC_Rad50)

162H

ATP binding site
43G, 44S, 46K, 47T, 48T, 162H
CDD
cd03240 (ABC_Rad50)
Q-loop/lid cd03240

159I

Q-loop/lid
159I, 160F, 161C, 162H
CDD
cd03240 (ABC_Rad50)

160F

Q-loop/lid
159I, 160F, 161C, 162H
CDD
cd03240 (ABC_Rad50)

161C

Q-loop/lid
159I, 160F, 161C, 162H
CDD
cd03240 (ABC_Rad50)

162H

Q-loop/lid
159I, 160F, 161C, 162H
CDD
cd03240 (ABC_Rad50)
ABC transporter si...

1,215C

ABC transporter signature motif
1,215C, 1,216S, 1,217A, 1,218G, 1,219Q, 1,220K, 1,221V, 1,222L, 1,223A, 1,224S
CDD
cd03240 (ABC_Rad50)

1,216S

ABC transporter signature motif
1,215C, 1,216S, 1,217A, 1,218G, 1,219Q, 1,220K, 1,221V, 1,222L, 1,223A, 1,224S
CDD
cd03240 (ABC_Rad50)

1,217A

ABC transporter signature motif
1,215C, 1,216S, 1,217A, 1,218G, 1,219Q, 1,220K, 1,221V, 1,222L, 1,223A, 1,224S
CDD
cd03240 (ABC_Rad50)

1,218G

ABC transporter signature motif
1,215C, 1,216S, 1,217A, 1,218G, 1,219Q, 1,220K, 1,221V, 1,222L, 1,223A, 1,224S
CDD
cd03240 (ABC_Rad50)

1,219Q

ABC transporter signature motif
1,215C, 1,216S, 1,217A, 1,218G, 1,219Q, 1,220K, 1,221V, 1,222L, 1,223A, 1,224S
CDD
cd03240 (ABC_Rad50)

1,220K

ABC transporter signature motif
1,215C, 1,216S, 1,217A, 1,218G, 1,219Q, 1,220K, 1,221V, 1,222L, 1,223A, 1,224S
CDD
cd03240 (ABC_Rad50)

1,221V

ABC transporter signature motif
1,215C, 1,216S, 1,217A, 1,218G, 1,219Q, 1,220K, 1,221V, 1,222L, 1,223A, 1,224S
CDD
cd03240 (ABC_Rad50)

1,222L

ABC transporter signature motif
1,215C, 1,216S, 1,217A, 1,218G, 1,219Q, 1,220K, 1,221V, 1,222L, 1,223A, 1,224S
CDD
cd03240 (ABC_Rad50)

1,223A

ABC transporter signature motif
1,215C, 1,216S, 1,217A, 1,218G, 1,219Q, 1,220K, 1,221V, 1,222L, 1,223A, 1,224S
CDD
cd03240 (ABC_Rad50)

1,224S

ABC transporter signature motif
1,215C, 1,216S, 1,217A, 1,218G, 1,219Q, 1,220K, 1,221V, 1,222L, 1,223A, 1,224S
CDD
cd03240 (ABC_Rad50)
Walker B cd03240

1,241I

Walker B
1,241I, 1,242I, 1,243A, 1,244L, 1,245D, 1,246E
CDD
cd03240 (ABC_Rad50)

1,242I

Walker B
1,241I, 1,242I, 1,243A, 1,244L, 1,245D, 1,246E
CDD
cd03240 (ABC_Rad50)

1,243A

Walker B
1,241I, 1,242I, 1,243A, 1,244L, 1,245D, 1,246E
CDD
cd03240 (ABC_Rad50)

1,244L

Walker B
1,241I, 1,242I, 1,243A, 1,244L, 1,245D, 1,246E
CDD
cd03240 (ABC_Rad50)

1,245D

Walker B
1,241I, 1,242I, 1,243A, 1,244L, 1,245D, 1,246E
CDD
cd03240 (ABC_Rad50)

1,246E

Walker B
1,241I, 1,242I, 1,243A, 1,244L, 1,245D, 1,246E
CDD
cd03240 (ABC_Rad50)
ATP binding site c...

1,245D

ATP binding site
1,245D, 1,246E, 1,283H
CDD
cd03240 (ABC_Rad50)

1,246E

ATP binding site
1,245D, 1,246E, 1,283H
CDD
cd03240 (ABC_Rad50)

1,283H

ATP binding site
1,245D, 1,246E, 1,283H
CDD
cd03240 (ABC_Rad50)
D-loop cd03240

1,249T

D-loop
1,249T, 1,250N, 1,251L, 1,252D
CDD
cd03240 (ABC_Rad50)

1,250N

D-loop
1,249T, 1,250N, 1,251L, 1,252D
CDD
cd03240 (ABC_Rad50)

1,251L

D-loop
1,249T, 1,250N, 1,251L, 1,252D
CDD
cd03240 (ABC_Rad50)

1,252D

D-loop
1,249T, 1,250N, 1,251L, 1,252D
CDD
cd03240 (ABC_Rad50)
H-loop/switch regi...

1,279I

H-loop/switch region
1,279I, 1,280V, 1,281I, 1,282T, 1,283H, 1,284D, 1,285E
CDD
cd03240 (ABC_Rad50)

1,280V

H-loop/switch region
1,279I, 1,280V, 1,281I, 1,282T, 1,283H, 1,284D, 1,285E
CDD
cd03240 (ABC_Rad50)

1,281I

H-loop/switch region
1,279I, 1,280V, 1,281I, 1,282T, 1,283H, 1,284D, 1,285E
CDD
cd03240 (ABC_Rad50)

1,282T

H-loop/switch region
1,279I, 1,280V, 1,281I, 1,282T, 1,283H, 1,284D, 1,285E
CDD
cd03240 (ABC_Rad50)

1,283H

H-loop/switch region
1,279I, 1,280V, 1,281I, 1,282T, 1,283H, 1,284D, 1,285E
CDD
cd03240 (ABC_Rad50)

1,284D

H-loop/switch region
1,279I, 1,280V, 1,281I, 1,282T, 1,283H, 1,284D, 1,285E
CDD
cd03240 (ABC_Rad50)

1,285E

H-loop/switch region
1,279I, 1,280V, 1,281I, 1,282T, 1,283H, 1,284D, 1,285E
CDD
cd03240 (ABC_Rad50)

Protein sequence

>MCA_04503_1
MYELSLKSSIFKLSIQGVRSFDTSERETIQFGTPLTLIVGQNGSGKTTIIECLKYVTTGDLPPNSKGGAFIYDAKLAGDK
EVLAQVKLAFKNTNGVDMIATRSMQVTIKRTTRTFKTLEGQLLAIKDGTRRTISTRCAELDAQLPIYLGVSKAVLENVIF
CHQEDSLWPLSEPSVLKKKFDEIFESQKFAKALDNIKSLRKGYAVDVKLEEQHSNFLKANKEKAHKIGEQLKSLNKQIEE
YATEADKYERDIEYITDELDSLQTTKQKFMDVLEELDHLLHEIKVNNENISRLESNSEILSDSEESLQNSLDNFSNQANQ
WKKDLIDIDTKIKKEKSAQSDTIAHHEESRSKEGQLKAEYENHLNDIKRRYQQAKALTAELKGLSQNIKDKFAKHSQSDL
TEDDYSDLMHSLDELASRYRLELEGEKAKNAVEENKYTSKIQETTTEKLRHEQAVISLTKSVRDCTEKIASLNTTIDSIS
VTDGDLAYKESQLQEMSGFLENVTKTLETIKSEGKITKLEALLKAIGQEMDDLNQELSIINQESDNRAKLAYLQEELAKK
KNAHKSLISAKKEEFKRIDIDLTDDENSSVDAEKNLRDTIQRAQKVYDDSVHEDDSINREHSLIQSKLNMKNAQIEKLEK
EKKDLFDYINSVIEIDYKDYDSQLEEYSAKQKTLSSSISNISIFKDLNSQAVDFAKNQNKCVMCYREFHNHEKDTFLQLI
QEKDNRLTNVEQMQKELDEVTEIVEALTSIRQSVDRLNTIESVTLPYDLEEKKKLEKELDVAASKLESSKEAKEKAKMEL
ERVEQLRRAASDLTRSQNDINNIKSQMSSLQLQLKNDSDNFRSSSDLLLELSKKNEQSKKYRKELDQLIEARDNARSSMA
KYEKSISEQRSAVEKIKSQLKTQKDAEEEKRSLEKKRIELNDTIVAEKEKMKSIQTLILEQEGERNELRRKGAERERDLA
LKYSDLLDSVKDIKRISESISEYEKQGGKENLIQCQSVVKTLADQLKASSSKIEQLNKKLNDTEKALVDLRNRERVLKDN
LEVKRLKRENSNKSARVKELNKQNAELERDKFRQRVGILQKEKDRLSTLHAGKMGELIRMRNQAQELKNELETEYKNVEK
EYLESLIKFRTTNVANEDLAKYSKALDNAIMKYHSLKMEEINRIIDELWKHTYSGTDVDTILIRSDNETTRGNRAYNYRV
CMVKKDVELDMRGRCSAGQKVLASIIIRLALAECFGTSCGIIALDEPTTNLDSDNIESLAQALNNLIATRRVQKNFQLIV
ITHDEKFLTHMNAASYTDHFFRVSRNERQTSQIEWVPISKIME

GO term prediction

Biological Process

GO:0000723 telomere maintenance
GO:0006281 DNA repair

Molecular Function

GO:0016887 ATPase activity

Cellular Component

GO:0005634 nucleus
GO:0030870 Mre11 complex

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Entry type

Status

Per-residue features