Protein

MCA_04447_1

Length
212 amino acids

Gene name: RPB5

Description: DNA-directed RNA polymerases I, II, and III subunit RPABC1

Browser: contigC:3076176-3076815+

RNA-seq: read pairs 1121, FPKM 65.0, percentile rank 70.9% (100% = highest expression)

Protein function

Annotation:RPB5DNA-directed RNA polymerases I, II, and III subunit RPABC1
KEGG:K03013RPB5 DNA-directed RNA polymerases I, II, and III subunit RPABC1
EGGNOG:0PN0DRPB5DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I, II and III which synthesize ribosomal RNA precursors, mRNA precursors and many functional non-coding RNAs, and small RNAs, such as 5S rRNA and tRNAs, respectively. Pol II is the central component of the basal RNA polymerase II transcription machinery. Pols are composed of mobile elements that move relative to each other. In Pol II, RPB5 is part of the lower jaw surrounding the central large cleft and thought to grab the incoming DNA template. Seems to be the major component in this process (By similarity)
SGD closest match:S000000358RPB5DNA-directed RNA polymerases I, II, and III subunit RPABC1
CGD closest match:CAL0000198535orf19.6340DNA-directed RNA polymerase core subunit

Protein alignments

%idAln lengthE-value
MIA_03636_181.90%2103e-131MIA_03636_1
A0A0J9XBM8_GEOCN78.57%2101e-127Similar to Saccharomyces cerevisiae YBR154C RPB5 RNA polymerase subunit ABC27 common to RNA polymerases I,II, and III OS=Geotrichum candidum GN=BN980_GECA08s02639g PE=3 SV=1
A0A1E3PUJ8_9ASCO70.48%2104e-114DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_55351 PE=3 SV=1
A0A1D8PFJ8_CANAL69.05%2103e-113DNA-directed RNA polymerase core subunit OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=orf19.6340 PE=3 SV=1
UniRef50_B9W9S069.05%2103e-109DNA-directed RNA polymerases I, II and III subunit, putative n=7 Tax=Dikarya TaxID=451864 RepID=B9W9S0_CANDC
A0A060T955_BLAAD67.77%2114e-108ARAD1D08954p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D08954g PE=3 SV=1
RPAB1_YEAST65.40%2118e-108DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RPB5 PE=1 SV=1
A0A1E4TJC0_9ASCO64.76%2102e-105Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_76759 PE=3 SV=1
RPAB1_YARLI59.91%2122e-98DNA-directed RNA polymerases I, II, and III subunit RPABC1 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=RPB5 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.6840

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
1 20 40 60 80 100 120 140 160 180 200 212

Detailed signature matches

  1. Family IPR014381
    DNA-directed RNA polymerase RPB5 subunit, eukaryote/virus
    1. PIRSF000747 (RPB5)
  2. Family IPR020609
    Archaeal RpoH /eukaryotic RPB5 RNA polymerase subunit
    1. MF_00025 (RNApol_Rp...)
  3. Domain IPR005571
    RNA polymerase, Rpb5, N-terminal
    1. SSF53036 (Eukaryoti...)
    2. G3DSA:3.40.13...
    3. PF03871 (RNA_pol_Rp...)
  4. Domain IPR000783
    RNA polymerase, subunit H/Rpb5 C-terminal
    1. PD005155
    2. SSF55287 (RPB5-like...)
    3. G3DSA:3.90.94...
    4. PF01191 (RNA_pol_Rp...)
  5. SITE IPR020608
    RNA polymerase, subunit H/Rpb5, conserved site
    1. PS01110 (RNA_POL_H_...)

Protein sequence

>MCA_04447_1
MDERSASRLWRIYRTTKEMVRDRGYNISQQEISISLEEFRSQFCDSMGNVNRKMMCYQAFPTEEALAKFPELGSLWVEFC
DEPSVGVNTMKSLCQHVLEKNFSTAIFVYQVSLSPGAVKSIPAVAPATIDTFQENDLVVNITHHVLVPKHIVLSKEEKKE
LLSRYKLKESQLPRVQKEDPIARYFGLKRGQVVKIMRLSPTAGRYASYRICL

GO term prediction

Biological Process

GO:0006351 transcription, DNA-templated

Molecular Function

GO:0003677 DNA binding
GO:0003899 DNA-directed 5'-3' RNA polymerase activity

Cellular Component

GO:0005634 nucleus