MCA_04330_1

Protein

Length

1,605 amino acids

Gene name: DNF1A

Description: Phospholipid-transporting ATPase DNF1; Aminophospholipid translocase (flippase)

Browser: contigC:2722431-2727249-

RNA-seq: read pairs 2445, FPKM 18.8, percentile rank 39.8% (100% = highest expression)

Protein function

Annotation:		DNF1A	Phospholipid-transporting ATPase DNF1; Aminophospholipid translocase (flippase)
KEGG:	K01530	E3.6.3.1	phospholipid-translocating ATPase [EC:3.6.3.1]
EGGNOG:	0PG3H	FG08595.1	Phospholipid-translocating P-type ATPase domain-containing protein
SGD closest match:	S000000968	DNF1	Phospholipid-transporting ATPase DNF1
CGD closest match:	CAL0000173967	orf19.932	Phospholipid-transporting ATPase

Protein alignments

	%id	Aln length
MIA_00461_1	68.75%	1453	MIA_00461_1
A0A0J9X7A2_GEOCN	62.53%	1465	Phospholipid-transporting ATPase OS=Geotrichum candidum GN=BN980_GECA04s03640g PE=3 SV=1
A0A060TDY5_BLAAD	57.06%	1430	Phospholipid-transporting ATPase OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D11220g PE=3 SV=1
A0A167DMY9_9ASCO	58.87%	1359	Phospholipid-transporting ATPase OS=Sugiyamaella lignohabitans GN=DNF1 PE=3 SV=1
Q6C5L2_YARLI	52.49%	1408	Phospholipid-transporting ATPase OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E17105g PE=3 SV=2
UniRef50_Q6C5L2	52.49%	1408	Phospholipid-transporting ATPase n=2 Tax=Yarrowia lipolytica TaxID=4952 RepID=Q6C5L2_YARLI
A0A1E3PGA2_9ASCO	52.58%	1358	Phospholipid-transporting ATPase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_83856 PE=3 SV=1
ATC5_YEAST	49.49%	1467	Phospholipid-transporting ATPase DNF1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=DNF1 PE=1 SV=2
A0A1E4TDZ8_9ASCO	52.22%	1352	Phospholipid-transporting ATPase OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_26222 PE=3 SV=1
A0A1D8PMY6_CANAL	48.30%	1410	Phospholipid-transporting ATPase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=orf19.932 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0068

Protein family membership

P-type ATPase (IPR001757)
- P-type ATPase, subfamily IV (IPR006539)

Domains and repeats

Domain
232 - 290
P-type ATPase, N-terminal (IPR032631)
469 - 529
P-type ATPase, A domain (IPR008250)
577 - 615
P-type ATPase, A domain (IPR008250)
743 - 1,090
HAD-like domain (IPR023214)
P-type ATPase, cytoplasmic domain N (IPR023299)
1,169 - 1,268
HAD-like domain (IPR023214)
Domain
238 - 330
P-type ATPase, transmembrane domain (IPR023298)
619 - 742
P-type ATPase, transmembrane domain (IPR023298)
1,262 - 1,490
P-type ATPase, transmembrane domain (IPR023298)
Domain
1,241 - 1,490
P-type ATPase, C-terminal (IPR032630)

1 200 400 600 800 1000 1200 1400 1605

Detailed signature matches

IPR001757

P-type ATPase
1. TIGR01494
  
  1,183 - 1,292
  
  TIGRFAMs
  TIGR01494 (TIGR01494)
IPR006539

P-type ATPase, subfamily IV
1. TIGR01652
  
  243 - 1,498
  
  TIGRFAMs
  TIGR01652 (TIGR01652)
IPR032631

P-type ATPase, N-terminal
1. PF16209 (PhoLip_ATP...)
  
  232 - 290
  
  Pfam
  PF16209 (PhoLip_ATPase_N)
IPR023298

P-type ATPase, transmembrane domain
1. SSF81665 (Calcium A...)
  
  238 - 330
  
  SUPERFAMILY
  SSF81665 (Calcium ATPase, transmembrane domain M)
  
  619 - 742
  
  SUPERFAMILY
  SSF81665 (Calcium ATPase, transmembrane domain M)
  
  1,262 - 1,490
  
  SUPERFAMILY
  SSF81665 (Calcium ATPase, transmembrane domain M)
IPR008250

P-type ATPase, A domain
1. SSF81653 (Calcium A...)
  
  577 - 615
  
  SUPERFAMILY
  SSF81653 (Calcium ATPase, transduction domain A)
  
  469 - 529
  
  SUPERFAMILY
  SSF81653 (Calcium ATPase, transduction domain A)
IPR023214

HAD-like domain
1. SSF56784 (HAD-like)
  
  981 - 1,090
  
  SUPERFAMILY
  SSF56784 (HAD-like)
  
  743 - 759
  
  SUPERFAMILY
  SSF56784 (HAD-like)
  
  1,169 - 1,268
  
  SUPERFAMILY
  SSF56784 (HAD-like)
IPR023299

P-type ATPase, cytoplasmic domain N
1. SSF81660 (Metal cat...)
  
  754 - 770
  
  SUPERFAMILY
  SSF81660 (Metal cation-transporting ATPase, ATP-binding domain N)
  
  858 - 1,056
  
  SUPERFAMILY
  SSF81660 (Metal cation-transporting ATPase, ATP-binding domain N)
2. G3DSA:3.40.11...
  
  743 - 1,050
  
  GENE3D
  G3DSA:3.40.1110.10 (G3DSA:3.40.1110.10)
IPR032630

P-type ATPase, C-terminal
1. PF16212 (PhoLip_ATP...)
  
  1,241 - 1,490
  
  Pfam
  PF16212 (PhoLip_ATPase_C)
IPR018303

P-type ATPase, phosphorylation site
1. PS00154 (ATPASE_E1_E2)
  
  750 - 756
  
  PROSITE patterns
  PS00154 (ATPASE_E1_E2)

no IPR

Unintegrated signatures

CYTOPLASMIC_D... (C...)

703 - 1,275

PHOBIUS
CYTOPLASMIC_DOMAIN (Cytoplasmic domain)

1,326 - 1,354

PHOBIUS
CYTOPLASMIC_DOMAIN (Cytoplasmic domain)

308 - 631

PHOBIUS
CYTOPLASMIC_DOMAIN (Cytoplasmic domain)

1,482 - 1,605

PHOBIUS
CYTOPLASMIC_DOMAIN (Cytoplasmic domain)

1,415 - 1,420

PHOBIUS
CYTOPLASMIC_DOMAIN (Cytoplasmic domain)
Coil

1,523 - 1,543

COILS
Coil (Coil)

1,019 - 1,039

COILS
Coil (Coil)
G3DSA:1.20.11...

1,110 - 1,496

GENE3D
G3DSA:1.20.1110.10 (G3DSA:1.20.1110.10)
G3DSA:2.70.15...

286 - 619

GENE3D
G3DSA:2.70.150.10 (G3DSA:2.70.150.10)
NON_CYTOPLASM... (N...)

1,444 - 1,462

PHOBIUS
NON_CYTOPLASMIC_DOMAIN (Non cytoplasmic domain)

1,384 - 1,394

PHOBIUS
NON_CYTOPLASMIC_DOMAIN (Non cytoplasmic domain)

659 - 677

PHOBIUS
NON_CYTOPLASMIC_DOMAIN (Non cytoplasmic domain)

1 - 273

PHOBIUS
NON_CYTOPLASMIC_DOMAIN (Non cytoplasmic domain)

1,293 - 1,303

PHOBIUS
NON_CYTOPLASMIC_DOMAIN (Non cytoplasmic domain)
PF00122 (E1-E2_ATPase)

474 - 707

Pfam
PF00122 (E1-E2_ATPase)
PF13246 (Cation_ATPase)

887 - 968

Pfam
PF13246 (Cation_ATPase)
PR00119 (CATATPASE)

748 - 762

PRINTS
PR00119 (CATATPASE)

1,213 - 1,232

PRINTS
PR00119 (CATATPASE)
SFLDF00027 (p-type_...)

730 - 1,266

SFLD
SFLDF00027 (p-type_atpase)
SFLDG00002 (C1.7:_P...)

730 - 1,266

SFLD
SFLDG00002 (C1.7:_P-type_atpase_like)
TMhelix

1,355 - 1,377

TMHMM
TMhelix (TMhelix)

634 - 656

TMHMM
TMhelix (TMhelix)

682 - 704

TMHMM
TMhelix (TMhelix)

1,421 - 1,443

TMHMM
TMhelix (TMhelix)

1,392 - 1,414

TMHMM
TMhelix (TMhelix)

1,303 - 1,325

TMHMM
TMhelix (TMhelix)

1,276 - 1,293

TMHMM
TMhelix (TMhelix)

273 - 307

TMHMM
TMhelix (TMhelix)

1,458 - 1,480

TMHMM
TMhelix (TMhelix)
TRANSMEMBRANE (Tran...)

632 - 658

PHOBIUS
TRANSMEMBRANE (Transmembrane region)

1,276 - 1,292

PHOBIUS
TRANSMEMBRANE (Transmembrane region)

1,463 - 1,481

PHOBIUS
TRANSMEMBRANE (Transmembrane region)

274 - 307

PHOBIUS
TRANSMEMBRANE (Transmembrane region)

1,304 - 1,325

PHOBIUS
TRANSMEMBRANE (Transmembrane region)

1,421 - 1,443

PHOBIUS
TRANSMEMBRANE (Transmembrane region)

1,355 - 1,383

PHOBIUS
TRANSMEMBRANE (Transmembrane region)

1,395 - 1,414

PHOBIUS
TRANSMEMBRANE (Transmembrane region)

678 - 702

PHOBIUS
TRANSMEMBRANE (Transmembrane region)
cd02073 (P-type_ATP...)

245 - 1,373

CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)
mobidb-lite (disord...)

142 - 178

MobiDB Lite
mobidb-lite (disorder_prediction)

1,586 - 1,605

MobiDB Lite
mobidb-lite (disorder_prediction)

84 - 128

MobiDB Lite
mobidb-lite (disorder_prediction)

1 - 61

MobiDB Lite
mobidb-lite (disorder_prediction)

1,534 - 1,580

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

phosphorylation si...

750D

phosphorylation site
750D
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)
P-type ATPase sign...

750D

P-type ATPase signature motif
750D, 751K, 752T, 753G, 754T, 755L, 756T
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

751K

P-type ATPase signature motif
750D, 751K, 752T, 753G, 754T, 755L, 756T
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

752T

P-type ATPase signature motif
750D, 751K, 752T, 753G, 754T, 755L, 756T
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

753G

P-type ATPase signature motif
750D, 751K, 752T, 753G, 754T, 755L, 756T
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

754T

P-type ATPase signature motif
750D, 751K, 752T, 753G, 754T, 755L, 756T
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

755L

P-type ATPase signature motif
750D, 751K, 752T, 753G, 754T, 755L, 756T
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

756T

P-type ATPase signature motif
750D, 751K, 752T, 753G, 754T, 755L, 756T
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)
putative ATP bindi...

750D

putative ATP binding site
750D, 751K, 752T, 935F, 959K, 960G, 961A, 1,027E, 1,074T, 1,075G, 1,076D, 1,189R, 1,192P, 1,195K, 1,215D, 1,218N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

751K

putative ATP binding site
750D, 751K, 752T, 935F, 959K, 960G, 961A, 1,027E, 1,074T, 1,075G, 1,076D, 1,189R, 1,192P, 1,195K, 1,215D, 1,218N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

752T

putative ATP binding site
750D, 751K, 752T, 935F, 959K, 960G, 961A, 1,027E, 1,074T, 1,075G, 1,076D, 1,189R, 1,192P, 1,195K, 1,215D, 1,218N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

935F

putative ATP binding site
750D, 751K, 752T, 935F, 959K, 960G, 961A, 1,027E, 1,074T, 1,075G, 1,076D, 1,189R, 1,192P, 1,195K, 1,215D, 1,218N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

959K

putative ATP binding site
750D, 751K, 752T, 935F, 959K, 960G, 961A, 1,027E, 1,074T, 1,075G, 1,076D, 1,189R, 1,192P, 1,195K, 1,215D, 1,218N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

960G

putative ATP binding site
750D, 751K, 752T, 935F, 959K, 960G, 961A, 1,027E, 1,074T, 1,075G, 1,076D, 1,189R, 1,192P, 1,195K, 1,215D, 1,218N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

961A

putative ATP binding site
750D, 751K, 752T, 935F, 959K, 960G, 961A, 1,027E, 1,074T, 1,075G, 1,076D, 1,189R, 1,192P, 1,195K, 1,215D, 1,218N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

1,027E

putative ATP binding site
750D, 751K, 752T, 935F, 959K, 960G, 961A, 1,027E, 1,074T, 1,075G, 1,076D, 1,189R, 1,192P, 1,195K, 1,215D, 1,218N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

1,074T

putative ATP binding site
750D, 751K, 752T, 935F, 959K, 960G, 961A, 1,027E, 1,074T, 1,075G, 1,076D, 1,189R, 1,192P, 1,195K, 1,215D, 1,218N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

1,075G

putative ATP binding site
750D, 751K, 752T, 935F, 959K, 960G, 961A, 1,027E, 1,074T, 1,075G, 1,076D, 1,189R, 1,192P, 1,195K, 1,215D, 1,218N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

1,076D

putative ATP binding site
750D, 751K, 752T, 935F, 959K, 960G, 961A, 1,027E, 1,074T, 1,075G, 1,076D, 1,189R, 1,192P, 1,195K, 1,215D, 1,218N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

1,189R

putative ATP binding site
750D, 751K, 752T, 935F, 959K, 960G, 961A, 1,027E, 1,074T, 1,075G, 1,076D, 1,189R, 1,192P, 1,195K, 1,215D, 1,218N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

1,192P

putative ATP binding site
750D, 751K, 752T, 935F, 959K, 960G, 961A, 1,027E, 1,074T, 1,075G, 1,076D, 1,189R, 1,192P, 1,195K, 1,215D, 1,218N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

1,195K

putative ATP binding site
750D, 751K, 752T, 935F, 959K, 960G, 961A, 1,027E, 1,074T, 1,075G, 1,076D, 1,189R, 1,192P, 1,195K, 1,215D, 1,218N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

1,215D

putative ATP binding site
750D, 751K, 752T, 935F, 959K, 960G, 961A, 1,027E, 1,074T, 1,075G, 1,076D, 1,189R, 1,192P, 1,195K, 1,215D, 1,218N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)

1,218N

putative ATP binding site
750D, 751K, 752T, 935F, 959K, 960G, 961A, 1,027E, 1,074T, 1,075G, 1,076D, 1,189R, 1,192P, 1,195K, 1,215D, 1,218N
CDD
cd02073 (P-type_ATPase_APLT_Dnf-like)
SFLDF00027

750D

SFLD_Res01
750D, 751K, 752T, 753G, 754T, 1,074T, 1,195K, 1,215D, 1,219D
SFLD
SFLDF00027 (p-type_atpase)

751K

SFLD_Res01
750D, 751K, 752T, 753G, 754T, 1,074T, 1,195K, 1,215D, 1,219D
SFLD
SFLDF00027 (p-type_atpase)

752T

SFLD_Res01
750D, 751K, 752T, 753G, 754T, 1,074T, 1,195K, 1,215D, 1,219D
SFLD
SFLDF00027 (p-type_atpase)

753G

SFLD_Res01
750D, 751K, 752T, 753G, 754T, 1,074T, 1,195K, 1,215D, 1,219D
SFLD
SFLDF00027 (p-type_atpase)

754T

SFLD_Res01
750D, 751K, 752T, 753G, 754T, 1,074T, 1,195K, 1,215D, 1,219D
SFLD
SFLDF00027 (p-type_atpase)

1,074T

SFLD_Res01
750D, 751K, 752T, 753G, 754T, 1,074T, 1,195K, 1,215D, 1,219D
SFLD
SFLDF00027 (p-type_atpase)

1,195K

SFLD_Res01
750D, 751K, 752T, 753G, 754T, 1,074T, 1,195K, 1,215D, 1,219D
SFLD
SFLDF00027 (p-type_atpase)

1,215D

SFLD_Res01
750D, 751K, 752T, 753G, 754T, 1,074T, 1,195K, 1,215D, 1,219D
SFLD
SFLDF00027 (p-type_atpase)

1,219D

SFLD_Res01
750D, 751K, 752T, 753G, 754T, 1,074T, 1,195K, 1,215D, 1,219D
SFLD
SFLDF00027 (p-type_atpase)
SFLDG00002

750D

SFLD_Res01
750D, 751K, 752T, 753G, 754T, 1,074T, 1,195K, 1,215D, 1,219D
SFLD
SFLDG00002 (C1.7:_P-type_atpase_like)

751K

SFLD_Res01
750D, 751K, 752T, 753G, 754T, 1,074T, 1,195K, 1,215D, 1,219D
SFLD
SFLDG00002 (C1.7:_P-type_atpase_like)

752T

SFLD_Res01
750D, 751K, 752T, 753G, 754T, 1,074T, 1,195K, 1,215D, 1,219D
SFLD
SFLDG00002 (C1.7:_P-type_atpase_like)

753G

SFLD_Res01
750D, 751K, 752T, 753G, 754T, 1,074T, 1,195K, 1,215D, 1,219D
SFLD
SFLDG00002 (C1.7:_P-type_atpase_like)

754T

SFLD_Res01
750D, 751K, 752T, 753G, 754T, 1,074T, 1,195K, 1,215D, 1,219D
SFLD
SFLDG00002 (C1.7:_P-type_atpase_like)

1,074T

SFLD_Res01
750D, 751K, 752T, 753G, 754T, 1,074T, 1,195K, 1,215D, 1,219D
SFLD
SFLDG00002 (C1.7:_P-type_atpase_like)

1,195K

SFLD_Res01
750D, 751K, 752T, 753G, 754T, 1,074T, 1,195K, 1,215D, 1,219D
SFLD
SFLDG00002 (C1.7:_P-type_atpase_like)

1,215D

SFLD_Res01
750D, 751K, 752T, 753G, 754T, 1,074T, 1,195K, 1,215D, 1,219D
SFLD
SFLDG00002 (C1.7:_P-type_atpase_like)

1,219D

SFLD_Res01
750D, 751K, 752T, 753G, 754T, 1,074T, 1,195K, 1,215D, 1,219D
SFLD
SFLDG00002 (C1.7:_P-type_atpase_like)

Protein sequence

>MCA_04330_1
MGIHNNFHSDEQPSSSNQPNNVADGDYINNPNDVEINRSTSNKRSVISETSSYDNLQNTSTKDNIEDSVFLSNHQQHQPP
EIFADDESISNPYSPNLQDKFHNSYEPKGSPNGAVDLSSGSSPYSGKIQQEFVPTDDLQHSNADKHINFTDPEQNGTQQH
NGEKRSHRFPSQKIKKRHSLTNQLGRSTSRAFRRISKHIPVVQKYVHDDDDELNIDTERTIYFNQELPEYEKDPETGGLL
HEYPRNKIRTSKYTPLNFIPLNLYHQFHNIANIYFLFIVILGAFPIFGVLDAALAAVPIIAIVVITAIKDAIEDYRRTVL
DLEVNNTMTHKLMGISNPNIEEDIINPWRRFKKASTKTSVRMLRACTRAFNKTFRKEKVRQQELMEKQLRKQRAQNQRHL
ETIGEGVELHAVQTRATVFSHDSASIYDATRLHGNSLDLDRYGVKSKVTLQKDGTVLDRLKPSAGHAKFHRAYWKQVNVG
DIVKLYNDDEVPADVIILSTSDDDGACYIETRNLDGETNLKVRQSLKATQAIRHARDLEQCSFQVESEGPHLDLYSYSGV
VKWKQRQDPLRFESPVVDRAEAVSISNMLLRGCTLRNTKWVIGIVIYSGRDSKIMMNSGITPSKRSRLTRELNIYVVINF
FILFIFALITGILNGISFRSERTSKRWFEFGSIGSTPAVNGIITFWAAVILLQNLIPISLYITIEIVKSFQAFFIYQDCH
MYYGPIDYRCTPKSWSISDDLGQIEYIFSDKTGTLTQNVMEFKKCTINGRSYGRAFTEAMVGIMKREGTYTDEIAQAKRQ
EIIEDKQLMLNKLRRTLSQVGSRNSIDLMDNLDDQEVTFVSSEMIDHLQGGMGPEQAQSIYHFLLVLSLCHAVLPERAKD
GSNKLIYKAQSPDESALVSFARDMGFALTERTRNGVIINEQGTTHEYEVLQSLEFNSARKRMSVIVRMPGTGKIYLFCKG
ADNVIYSRLKPNCQHELRQATSDQLEVFAKEGLRTLCLAQKELSVEEYNDWAARHELASQALNNREEQMEMVADEIERDL
YLIGGTAIEDRLQDGVPETIELLSRAGIKLWVLTGDKVETAINIGFSCNLLDPGMELIFLQFPTKTLEYAIKDIDDKLQK
YFGLEYNEETMKKAREDHSVPSREYAIILDGDSLELIGSAEPDEDATRKGLRERFVLLCKQCRSVLCCRVSPSQKALVVS
SVKSTLDVTTLAIGDGANDVAMIQEADVGVGIAGLEGRQAVMSSDYGFGQFRFLSRLVLVHGRWAYRRVAEMTANMFYKN
VVFTLTLFWYNVHNDFDCSYLFDYTYITLFNLAFTSLPVIFMGFLDQDVNDKISLAVPELYRRGILRKEWTQWKFWIYIT
DGIYQSAVCYFLAYCMIYNGGFITENGRQNAYREGYGTLVATSAILACDFYVLINEYMWDWVFGLIVAISILLVFFWTGV
YTAFTASSWFYKAAPEVYGALGFWAYLLLATLACLLPRWSAKCYQKMFMPRDIDIIREQWKNHEFDYLKEEPIDEDEEDQ
RMFELSEMQKQRLEEDRQYLQEEQSAGSSNNNNINQQNPYDIQHNNNGSAMYNTNNNNNIKVHEDEDTATSSSYNGAATT
IQQQV

GO term prediction

Biological Process

GO:0015914 phospholipid transport

Molecular Function

GO:0000166 nucleotide binding
GO:0000287 magnesium ion binding
GO:0004012 phospholipid-translocating ATPase activity
GO:0005524 ATP binding

Cellular Component

GO:0016021 integral component of membrane

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Entry type

Status

Per-residue features