Protein

MCA_03577_1

Length
1,730 amino acids

Gene name: TOP2

Description: DNA topoisomerase 2

Browser: contigC:389463-394721-

RNA-seq: read pairs 1937, FPKM 13.8, percentile rank 32.2% (100% = highest expression)

Protein function

Annotation:TOP2DNA topoisomerase 2
KEGG:K03164TOP2 DNA topoisomerase II [EC:5.99.1.3]
EGGNOG:0PG8VTOP2Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks (By similarity)
SGD closest match:S000005032TOP2DNA topoisomerase 2
CGD closest match:CAL0000175071TOP2DNA topoisomerase 2

Protein alignments

%idAln lengthE-value
MIA_03718_180.05%11780.0MIA_03718_1
A0A0J9X495_GEOCN74.40%11720.0DNA topoisomerase 2 OS=Geotrichum candidum GN=BN980_GECA02s07776g PE=3 SV=1
A0A060TAB6_BLAAD66.27%11860.0DNA topoisomerase 2 OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D22572g PE=3 SV=1
A0A1E3PNV1_9ASCO67.23%11810.0DNA topoisomerase 2 (Fragment) OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_21822 PE=3 SV=1
UniRef50_W1Q6Y464.02%11840.0DNA topoisomerase 2 n=8 Tax=Dikarya TaxID=451864 RepID=W1Q6Y4_OGAPD
Q6C7Z9_YARLI63.17%11810.0DNA topoisomerase 2 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_D23969g PE=3 SV=1
A0A1D8PMM1_CANAL63.03%11930.0DNA topoisomerase 2 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=TOP2 PE=3 SV=1
A0A1E4TJ76_9ASCO60.05%12040.0DNA topoisomerase 2 OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_30135 PE=3 SV=1
TOP2_YEAST62.05%11780.0DNA topoisomerase 2 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TOP2 PE=1 SV=2
A0A167CGU8_9ASCO65.66%10310.0DNA topoisomerase 2 OS=Sugiyamaella lignohabitans GN=TOP2 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0964

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
  3. Domain
  4. Domain
1 200 400 600 800 1000 1200 1400 1600 1730

Detailed signature matches

  1. Family IPR001241
    DNA topoisomerase, type IIA
    1. PR00418 (TPI2FAMILY)
    2. SM00433 (topII5)
  2. Family IPR001154
    DNA topoisomerase II, eukaryotic-type
    1. PR01158 (TOPISMRASEII)
  3. Domain IPR003594
    Histidine kinase-like ATPase, C-terminal domain
    1. G3DSA:3.30.56...
    2. PF02518 (HATPase_c)
    3. SSF55874 (ATPase do...)
    4. SM00387 (HKATPase_4)
  4. Domain IPR020568
    Ribosomal protein S5 domain 2-type fold
    1. SSF54211 (Ribosomal...)
  5. Domain IPR014721
    Ribosomal protein S5 domain 2-type fold, subgroup
    1. G3DSA:3.30.23...
  6. Domain IPR013506
    DNA topoisomerase, type IIA, subunit B, domain 2
    1. PF00204 (DNA_gyraseB)
  7. Domain IPR013760
    DNA topoisomerase, type IIA-like domain
    1. SSF56719 (Type II D...)
  8. Domain IPR013759
    DNA topoisomerase, type IIA, central domain
    1. G3DSA:3.40.50...
  9. Domain IPR006171
    TOPRIM domain
    1. PF01751 (Toprim)
    2. PS50880 (TOPRIM)
  10. Domain IPR034157
    DNA topoisomerase 2, TOPRIM domain
    1. cd03365 (TOPRIM_Top...)
  11. Domain IPR031660
    C-terminal associated domain of TOPRIM
    1. PF16898 (TOPRIM_C)
  12. Domain IPR002205
    DNA topoisomerase, type IIA, subunit A/C-terminal
    1. SM00434 (topIV4)
    2. PF00521 (DNA_topoisoIV)
  13. Domain IPR013758
    DNA topoisomerase, type IIA, subunit A/ C-terminal, alpha-beta
    1. G3DSA:3.90.19...
  14. Domain IPR024946
    Arginine repressor C-terminal-like domain
    1. G3DSA:3.30.13...
  15. Domain IPR013757
    Type IIA DNA topoisomerase subunit A, alpha-helical domain
    1. G3DSA:1.10.26...
  16. SITE IPR018522
    DNA topoisomerase, type IIA, conserved site
    1. PS00177 (TOPOISOMER...)
Unintegrated signatures no IPR
Unintegrated signatures
  1. Coil
  2. G3DSA:3.30.14...
  3. cd00075 (HATPase_c)
  4. cd00187 (TOP4c)
  5. cd03481 (TopoIIA_Tr...)
  6. mobidb-lite (disord...)

Residue annotation

  1. ATP binding site c...
  2. Mg2+ binding site ...
  3. G-X-G motif cd00075
  4. dimer interface cd...
  5. ATP binding cd03481
  6. putative metal-bin...
  7. active site cd03365
  8. CAP-like domain cd...
  9. Active site cd00187
  10. primary dimer inte...

Protein sequence

>MCA_03577_1
MADIFDSDDDFAVTSSKTTNSKPRASKAKSKTQTFQFSDSDLSDGIVEDETVPKPKKAAAPKKKAASKKTPLSEVSANGG
SKPSSKSSKKDDEDDDFDMDDVDDKENNIAPNGTSKKDASSQYQKLTQLEHILKRPDTYIGSVEPTESKMWIFNAETKSM
EYKDVTIVPGLYKIFDEILVNAADNKIRDPKMDRLDVTIDAEANTISVKNNGKGIPIEIHKKEKIYIPELIFGNLLTSSN
YDDDEKKVTGGRNGYGAKLCNIFSTEFKVETAEKKTKTIYSQSWSNNMSKVTKPVIKKGATATEYTKITFKPDLKLFHME
KIDKDLLGVLYRRVYDIAGCLSGCKVFLNGERLHIKDFKSYTGMFVEALEKDQDPSDPAGTGPKQPVIIHEKINDRWEIA
FAVSDGNFNQVSFVNSIATTSGGTHVQYIADQIVTKITEYIKKAKVPIKGFQVKNNMFLFINCLIENPAFTSQTKEQLTT
RSSAFGSKCILPPEFLKKIMKTPILDRVRDIATRNADREIRKTDGDRSRRIKGLAKLEDANKAGTREGYKCTLVLTEGDS
AMALAVAGLAVVGRDYYGCFPLRGKLLNVRDASHDQIMKNAEIQAIKQIMGLQHKKHYTDTKSLRYGHIMIMTDQDHDGS
HIKGLIINFLETTFPGLLEIPGFLVEFITPIIKVSVMRNKKVAEVIPFYTMPEYEEWRETVGKTCTWKKKYYKGLGTSDS
AEGREYFSLLDKHLKKFHALQSDDKPLIELAFSKKKADERKDWLRAFRPGTHLDPSLEVIPISEFINKELILFSIADNMR
SIPSMLDGLKPGQRKVLFGCFKRNLNSEIKVAQLAGYISEHTGYHHGEQSLVQTIVNLAQDYVGSNNLYLLLPKGSFGSR
AAGGKDSSAPRYIHTELNPITRKIFHPADENVYNYLEDDEATVEPEWYVPILPMILVNGAEGIGTGWSTNIPPYNPVDII
DNIRRLMNNEPMVPMMPWYRGWTGEITPIDSTKYRASGRIEQTSETTVEITELPPHTWTINMRDFLLNQLRDKKDNKSGW
IEDFTEDHGLGIKFEIKLTKEEMEKSLKEGLLNRFKLTSNINLSNMVAFDPQNRIKKYSTPEEIISDFYFIRLEFYQKRK
NFLSNQLHNQLTKLSEQARFVKMIIEKTLIVTNRKKVDIIADLERLDFPAFTKDGEPVFKPKLEVDEEESEEEDLPEDVE
DPRKYKPNYDYLLGMSIWSLTRERYEKLLRDRDVKEEELNILLRKSPKDLWNEDLDVFLEKWQEFLEDDREQREALLPKK
KGKGKGKGRTTGGKKKRKGVDSDDDDDDDFVISTKKKEPARAPSNRKKKKIEPIEDLEVDKDTKKEVKQKSVSPVDDSSK
SSSSSTKSKVQTTLDSFAVKKDDESDNKKSSGGGSSSVKQESPKPKPRTKAFIIDDDDDEIDDVFNSFVEGYEQFKAATS
GTKSDVKDEAISTSTTTVATKAPSIFGNSKFKSIFDSDDDGDDDLFNKSTKPKATYSKTTSSSTANTKKKTSSFFSDDEE
DDDLVSDKKKTTVDDDKKATTKNKNNKRATSKSKATIISDSDDDDELSEITPSPTPPPPVEATTVAPATKKAATTKRASK
KAAPKSKATVISDDEEDEDIVAPVKKTTKKKVAAPKSKATVISDEDDDDDAITEVVKKKKPAPKSKQNSKELLSLSDDDD
NNGDESIQITTSSRRPRRTTRKAAPKKVLISDDEMFSNDDDDDEYDEYEE

GO term prediction

Biological Process

GO:0006259 DNA metabolic process
GO:0006265 DNA topological change

Molecular Function

GO:0003677 DNA binding
GO:0003918 DNA topoisomerase type II (ATP-hydrolyzing) activity
GO:0005524 ATP binding

Cellular Component

None predicted.