MCA_03512_1

Protein

Length

1,603 amino acids

Description: Retrotransposon Ty3/Gypsy Gag-Pol polyprotein

RNA-seq: read pairs 7, FPKM 0.1, percentile rank 2.6% (100% = highest expression)

Protein function

Annotation:			Retrotransposon Ty3/Gypsy Gag-Pol polyprotein
EGGNOG:	0QDZF		Reverse transcriptase (RNA-dependent DNA polymerase)
CGD closest match:	CAL0000201372	orf19.2372	Uncharacterized protein

Protein alignments

	%id	Aln length	E-value
MIA_00003_1	39.11%	496	4e-99	MIA_00003_1
UniRef50_A5DXY5	38.84%	502	7e-93	Uncharacterized protein n=2 Tax=Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239) TaxID=379508 RepID=A5DXY5_LODEL
A0A161HJI4_9ASCO	29.09%	416	7e-47	Gag-pol fusion protein OS=Sugiyamaella lignohabitans GN=AWJ20_4398 PE=4 SV=1
YCH4_YEAST	35.96%	317	2e-44	Putative transposon Ty5-1 protein YCL074W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TY5A PE=5 SV=2
A0A1D8PR73_CANAL	26.44%	522	3e-40	Uncharacterized protein OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=orf19.2372 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0136

Protein family membership

None predicted.

Domains and repeats

Domain
273 - 464
Ribonuclease H-like domain (IPR012337)
Integrase, catalytic core (IPR001584)
1,107 - 1,350
Reverse transcriptase, RNA-dependent DNA polymerase (IPR013103)

1 200 400 600 800 1000 1200 1400 1603

Detailed signature matches

IPR012337

Ribonuclease H-like domain
1. SSF53098 (Ribonucle...)
  
  280 - 454
  
  SUPERFAMILY
  SSF53098 (Ribonuclease H-like)
2. G3DSA:3.30.42...
  
  273 - 464
  
  GENE3D
  G3DSA:3.30.420.10 (G3DSA:3.30.420.10)
IPR001584

Integrase, catalytic core
1. PF00665 (rve)
  
  280 - 400
  
  Pfam
  PF00665 (rve)
2. PS50994 (INTEGRASE)
  
  278 - 448
  
  PROSITE profiles
  PS50994 (INTEGRASE)
IPR013103

Reverse transcriptase, RNA-dependent DNA polymerase
1. PF07727 (RVT_2)
  
  1,107 - 1,350
  
  Pfam
  PF07727 (RVT_2)

no IPR

Unintegrated signatures

SSF56672 (DNA/RNA p...)

1,109 - 1,330

SUPERFAMILY
SSF56672 (DNA/RNA polymerases)

1,362 - 1,542

SUPERFAMILY
SSF56672 (DNA/RNA polymerases)
cd09272 (RNase_HI_R...)

1,441 - 1,580

CDD
cd09272 (RNase_HI_RT_Ty1)
mobidb-lite (disord...)

132 - 169

MobiDB Lite
mobidb-lite (disorder_prediction)

604 - 659

MobiDB Lite
mobidb-lite (disorder_prediction)

837 - 929

MobiDB Lite
mobidb-lite (disorder_prediction)

696 - 809

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

active site cd09272

1,445D

active site
1,445D, 1,487E, 1,522D, 1,571D
CDD
cd09272 (RNase_HI_RT_Ty1)

1,487E

active site
1,445D, 1,487E, 1,522D, 1,571D
CDD
cd09272 (RNase_HI_RT_Ty1)

1,522D

active site
1,445D, 1,487E, 1,522D, 1,571D
CDD
cd09272 (RNase_HI_RT_Ty1)

1,571D

active site
1,445D, 1,487E, 1,522D, 1,571D
CDD
cd09272 (RNase_HI_RT_Ty1)
RNA/DNA hybrid bin...

1,445D

RNA/DNA hybrid binding site
1,445D, 1,446A, 1,447N, 1,448L, 1,482S, 1,483S, 1,484A, 1,487E, 1,522D, 1,548M, 1,575K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,446A

RNA/DNA hybrid binding site
1,445D, 1,446A, 1,447N, 1,448L, 1,482S, 1,483S, 1,484A, 1,487E, 1,522D, 1,548M, 1,575K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,447N

RNA/DNA hybrid binding site
1,445D, 1,446A, 1,447N, 1,448L, 1,482S, 1,483S, 1,484A, 1,487E, 1,522D, 1,548M, 1,575K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,448L

RNA/DNA hybrid binding site
1,445D, 1,446A, 1,447N, 1,448L, 1,482S, 1,483S, 1,484A, 1,487E, 1,522D, 1,548M, 1,575K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,482S

RNA/DNA hybrid binding site
1,445D, 1,446A, 1,447N, 1,448L, 1,482S, 1,483S, 1,484A, 1,487E, 1,522D, 1,548M, 1,575K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,483S

RNA/DNA hybrid binding site
1,445D, 1,446A, 1,447N, 1,448L, 1,482S, 1,483S, 1,484A, 1,487E, 1,522D, 1,548M, 1,575K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,484A

RNA/DNA hybrid binding site
1,445D, 1,446A, 1,447N, 1,448L, 1,482S, 1,483S, 1,484A, 1,487E, 1,522D, 1,548M, 1,575K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,487E

RNA/DNA hybrid binding site
1,445D, 1,446A, 1,447N, 1,448L, 1,482S, 1,483S, 1,484A, 1,487E, 1,522D, 1,548M, 1,575K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,522D

RNA/DNA hybrid binding site
1,445D, 1,446A, 1,447N, 1,448L, 1,482S, 1,483S, 1,484A, 1,487E, 1,522D, 1,548M, 1,575K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,548M

RNA/DNA hybrid binding site
1,445D, 1,446A, 1,447N, 1,448L, 1,482S, 1,483S, 1,484A, 1,487E, 1,522D, 1,548M, 1,575K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,575K

RNA/DNA hybrid binding site
1,445D, 1,446A, 1,447N, 1,448L, 1,482S, 1,483S, 1,484A, 1,487E, 1,522D, 1,548M, 1,575K
CDD
cd09272 (RNase_HI_RT_Ty1)

Protein sequence

>MCA_03512_1
MMFKVYDSDELDSETLIDSGASVAVINDKNLFYELEERKTVLDGPSGSGTVTSYGQGKVVYMLDNGEKPFLRSFNAYFVP
CSRYNLIPEGFLLEHLSIGGLGKSGVEESPRKCYNNKDEYIYGPNTNTITKKNQKISRTSENVKDNHTPVNDYTTSDNTE
NNESDGEVEHQEVGGEILFDTPDDDADFELDPDDANYSYGGNPLYNNDTSTSVFERRESPKTDSTNLEIRSTSQALSINN
QNIPITKYDEELIANCPFCSVSKFTRNFKPEEHANKFENSAPLSTIHADITGYYPYPDLSGNRYALTIVDGYSNYVTTIT
IQTKDQATQKLIDWINKQENYFRGKTNYNVKRLQCDNGSEFTNNEFRQFCSEKGIEITFSPPDEPSRNGKVERFNRTIEE
KAQAACQKAGLPHEFYWSFAKIHASLVYNYVALDSNNKSPYELFCGKAPNTKGLLPFGCRIDVPRTGGDLHSPRGCLAIY
LGQGSNGRLRLYECPFNGKMAITGSGKPHPTEFPGRNMTFDKSVLQTPLNERTYPRQSKTTNSYFSPTFVAGYMRNNAEV
DYFEESTFSGTDDEFYDNTFEGAEQAIEDEKIENLNRKYNNETYVNVSDDDTSENAEDESFSDSDQYGDCDDGGDETYET
ENDENGESSPNPDTTMTDAENFQQSNIGENQQNIINENINQPDTTRQTRNVHFEDPMDTDEDMQEESSVNSESEPSNESY
EYTIPRNNSDAEESSTTSSTTYSSPTRQQRQRRDDGFMMSTNEPTNSNTTEEETHERHVQSSRITELPNDSTPMPVTPSN
NRNYNDNRFDTPSVVEQVATSTPFQRENQAIDQQLISRNSNQQLVNTNSERQISTSNNERQLSTTNSNRQLSTTNSERQM
STTNSERQLSTTNSERQMSTTNSERQISMTNSERQVSTTNGERSIVPTNFQDRLAAIDEELNNRYFSNRPIEAPPERPLL
TNDDFQQQENNQMVSTNSNSNPYAEDTFMTETDGTIINYEDGVTITYETDTPEAPEVPVVEAPHRPVTRSMTRRLREQAG
NDEDIRFINHVSSSTIYKNSKQETFQVVKSIEGNPDFIRVPCREVPKQDSEKWLEKMDGEMVALKVNKVYKLVKLPPGFT
PLRVRWVLVKKYDANGDLERYKARLVVRGYLQKEGINYGETFSPVARYDTVRLVFAIATKFNHEVVHMDFTNAFQFGEID
YEIYVTQPPGYLAKGKEDHVLKLEKSLYGLKQAPLCWSRRLNKAISKLGYKATIKEPCLYVKEENGRISIICTYVDDLLV
STPIKGEVDRIYQAFTDEGLVVKNLGFPSKFLGMRVTKKDDTIRLDLKTYIKDLVEFAGLQHANPEITPFKSSVKLEKLK
PEEAGKPIKDPKLFRSIIGKLIFASNTCRPDISTAVSILSQYLKEPREAHLTCAKTVVKYLHNTADFSLKFKNTEGAPLV
VGYADANLTNNADSKSRSGYIIKIYGNIVSWMSKKQTRAAQSSAESELYALNKAVKEMLYFRDLLGYELGLQLEGASTIY
EDNQPCIDMAKQPKINARTRYIRSDHMMIHECVERNEISVEHIGTKKMLADGFTKILPAPEFRKFYEDLDLVPDDYQKRE
RNE

GO term prediction

Biological Process

GO:0015074 DNA integration

Molecular Function

GO:0003676 nucleic acid binding

Cellular Component