Protein

MCA_03201_1

Length
985 amino acids

Gene name: MIS1A

Description: C-1-tetrahydrofolate synthase, mitochondrial

Browser: contigB:3635669-3638711-

RNA-seq: read pairs 651, FPKM 8.2, percentile rank 22.7% (100% = highest expression)

Protein function

Annotation:MIS1AC-1-tetrahydrofolate synthase, mitochondrial
KEGG:K00288MTHFD methylenetetrahydrofolate dehydrogenase (NADP+) / methenyltetrahydrofolate cyclohydrolase / formyltetrahydrofolate synthetase [EC:1.5.1.5 3.5.4.9 6.3.4.3]
EGGNOG:0PFGEFG10267.1C-1-tetrahydrofolate synthase
SGD closest match:S000000288MIS1C-1-tetrahydrofolate synthase, mitochondrial
CGD closest match:CAL0000181460MIS11Trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase

Protein alignments

%idAln lengthE-value
MIA_03554_179.05%9930.0MIA_03554_1
A0A0J9YH90_GEOCN76.76%9810.0Similar to Saccharomyces cerevisiae YBR084W MIS1 Mitochondrial C1-tetrahydrofolate synthase OS=Geotrichum candidum GN=BN980_GECA01s00912g PE=3 SV=1
A0A060T747_BLAAD71.97%9560.0ARAD1C25828p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C25828g PE=3 SV=1
A0A1E3PST5_9ASCO73.57%9460.0C1-THFS protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_30651 PE=3 SV=1
A0A167DXR6_9ASCO71.77%9670.0Trifunctional formate-tetrahydrofolate ligase OS=Sugiyamaella lignohabitans GN=MIS1 PE=3 SV=1
F2Z650_YARLI73.41%9440.0YALI0E01056p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E01056g PE=3 SV=1
A0A1E4TJX2_9ASCO71.94%9410.0Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_603 PE=3 SV=1
Q59SM8_CANAL68.84%9500.0Trifunctional formate-tetrahydrofolate ligase/methenyltetrahydrofolate cyclohydrolase/methylenetetrahydrofolate dehydrogenase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=MIS11 PE=3 SV=1
UniRef50_A0A1V8U15568.75%9440.0C-1-tetrahydrofolate synthase, cytoplasmic n=1 Tax=Rachicladosporium sp. CCFEE 5018 TaxID=1974281 RepID=A0A1V8U155_9PEZI
C1TM_YEAST66.31%9470.0C-1-tetrahydrofolate synthase, mitochondrial OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=MIS1 PE=1 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.9980
Predicted cleavage: 42

Protein family membership

Domains and repeats

  1. Domain
1 100 200 300 400 500 600 700 800 900 985

Detailed signature matches

  1. Family IPR000672
    Tetrahydrofolate dehydrogenase/cyclohydrolase
    1. MF_01576 (THF_DHG_CYH)
    2. PR00085 (THFDHDRGNASE)
  2. Family IPR000559
    Formate-tetrahydrofolate ligase, FTHFS
    1. cd00477 (FTHFS)
    2. PF01268 (FTHFS)
    3. MF_01543 (FTHFS)
  3. Domain IPR020630
    Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain
    1. PF00763 (THF_DHG_CYH)
  4. Domain IPR016040
    NAD(P)-binding domain
    1. SSF51735 (NAD(P)-bi...)
  5. Domain IPR020631
    Tetrahydrofolate dehydrogenase/cyclohydrolase, NAD(P)-binding domain
    1. PF02882 (THF_DHG_CYH_C)
  6. Domain IPR027417
    P-loop containing nucleoside triphosphate hydrolase
    1. SSF52540 (P-loop co...)
  7. SITE IPR020867
    Tetrahydrofolate dehydrogenase/cyclohydrolase, conserved site
    1. PS00766 (THF_DHG_CYH_1)
    2. PS00767 (THF_DHG_CYH_2)
  8. SITE IPR020628
    Formate-tetrahydrofolate ligase, FTHFS, conserved site
    1. PS00722 (FTHFS_2)
    2. PS00721 (FTHFS_1)
Unintegrated signatures no IPR
Unintegrated signatures
  1. G3DSA:3.10.41...
  2. G3DSA:3.40.50...
  3. G3DSA:3.40.50...
  4. G3DSA:3.40.50...
  5. SSF53223 (Aminoacid...)
  6. cd01080 (NAD_bind_m...)

Residue annotation

  1. homodimer interfac...
  2. NADP binding site ...
  3. substrate binding ...
  4. Cesium cation bind...
  5. Potassium binding ...

Protein sequence

>MCA_03201_1
MLSSVIRSSVLKAPLPRASLLRSTFRSVAAFRSFSQTSRIFNAALIDGKLIAKSIRENANKEIKQIQASNPTFNPSLVII
QIGSRPDSSAYVRMKLKAAKEADIDCEVRKLPDTITQAELLKQIHELNVNPKVHGILVQLPIPNPNIDETVITDAVAAEK
DVDGFGTLNIGELAKRGGNPGFVPCTPKGIMVLLEQSGVELRGKNAVVIGRSDIVGNPISYLLRDADATVTICHSKTKNL
PEVVKQADVVVSATGMPEFVKGDWLKPGAVVIDVGINYIPDSTKKSGKKLVGDVEFSSASKVASKITPVPGGVGPMTVAM
LIQNVLDSAKRSLDKESHRKINPLPLELKSPVPSDIDISRSQTPKFITEVSKEIGITEDEFEPYGAYKGKVSLSVLNRLN
ERKNGKYVLVAGITPTPLGEGKSTTTVGLVQALGAHLDKMAFATVRQPSMGPTFGIKGGAAGGGYSQVIPMDEFNMHLTG
DIHAISAANNLLAAAIDTRMFHETFQKDAALYKRLVPKKNGKRSFTKGMLKRLEKLGINKTDPDTLTPEEITKFARLDID
PETITWKRVVDCNDRMLRTITVGEGAAERGQTRQTGFDISVASECMAILALSTSIKDMRERLGRIVIGSSKSGEPITCDD
IGCAGALTALLKDAIKPNLMQTLEGTPVFVHAGPFANISIGASSVLADKIALKLAGTDAGEDYHEKAGYVVTEAGFDFTM
GGERFFNIKCRASGLRPDVVVIVATVRALKLHGGGPDVKPGSPLPEEYKTENLELVRKGAASNLRKQIENAKSYGSPVVV
AINRFVTDTDAELEAVRQAALEAGAFDSVATNHWEEGGAGAVALAQSVIEASKAEKTPEMNLLYTTDASSSSPEDKLRTI
AQKMYGAADIELSPIAKKKIETYTQQGFGHLPICIAKTQYSLSHDAALKGAPTGFTFPIRDLRASVGAGYLYALAAEIMT
IPGLPTHPGFMDVEVNDEGEIEGLF

GO term prediction

Biological Process

GO:0055114 oxidation-reduction process

Molecular Function

GO:0003824 catalytic activity
GO:0004329 formate-tetrahydrofolate ligase activity
GO:0004488 methylenetetrahydrofolate dehydrogenase (NADP+) activity
GO:0005524 ATP binding

Cellular Component

None predicted.