MCA_03184_1

Protein

Length

2,005 amino acids

Description: Reverse transcriptase

RNA-seq: read pairs 2, FPKM 0.0, percentile rank 1.6% (100% = highest expression)

Protein function

Annotation:			Reverse transcriptase
EGGNOG:	0QDZF		Reverse transcriptase (RNA-dependent DNA polymerase)
CGD closest match:	CAL0000201372	orf19.2372	Uncharacterized protein

Protein alignments

	%id	Aln length	E-value
MIA_00003_1	39.11%	496	6e-98	MIA_00003_1
UniRef50_A5DXY5	38.84%	502	2e-92	Uncharacterized protein n=2 Tax=Lodderomyces elongisporus (strain ATCC 11503 / CBS 2605 / JCM 1781 / NBRC 1676 / NRRL YB-4239) TaxID=379508 RepID=A5DXY5_LODEL
A0A161HJI4_9ASCO	29.09%	416	2e-46	Gag-pol fusion protein OS=Sugiyamaella lignohabitans GN=AWJ20_4398 PE=4 SV=1
YCH4_YEAST	35.96%	317	2e-44	Putative transposon Ty5-1 protein YCL074W OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TY5A PE=5 SV=2
A0A1D8PR73_CANAL	26.44%	522	7e-40	Uncharacterized protein OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=orf19.2372 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.1252

Protein family membership

None predicted.

Domains and repeats

Domain
675 - 865
Ribonuclease H-like domain (IPR012337)
Integrase, catalytic core (IPR001584)
1,509 - 1,752
Reverse transcriptase, RNA-dependent DNA polymerase (IPR013103)

1 200 400 600 800 1000 1200 1400 1600 1800 2005

Detailed signature matches

IPR012337

Ribonuclease H-like domain
1. SSF53098 (Ribonucle...)
  
  682 - 856
  
  SUPERFAMILY
  SSF53098 (Ribonuclease H-like)
2. G3DSA:3.30.42...
  
  675 - 865
  
  GENE3D
  G3DSA:3.30.420.10 (G3DSA:3.30.420.10)
IPR001584

Integrase, catalytic core
1. PF00665 (rve)
  
  682 - 802
  
  Pfam
  PF00665 (rve)
2. PS50994 (INTEGRASE)
  
  680 - 850
  
  PROSITE profiles
  PS50994 (INTEGRASE)
IPR013103

Reverse transcriptase, RNA-dependent DNA polymerase
1. PF07727 (RVT_2)
  
  1,509 - 1,752
  
  Pfam
  PF07727 (RVT_2)

no IPR

Unintegrated signatures

SSF56672 (DNA/RNA p...)

1,764 - 1,944

SUPERFAMILY
SSF56672 (DNA/RNA polymerases)

1,511 - 1,732

SUPERFAMILY
SSF56672 (DNA/RNA polymerases)
cd09272 (RNase_HI_R...)

1,843 - 1,982

CDD
cd09272 (RNase_HI_RT_Ty1)
mobidb-lite (disord...)

512 - 555

MobiDB Lite
mobidb-lite (disorder_prediction)

1,005 - 1,061

MobiDB Lite
mobidb-lite (disorder_prediction)

225 - 284

MobiDB Lite
mobidb-lite (disorder_prediction)

1,098 - 1,211

MobiDB Lite
mobidb-lite (disorder_prediction)

1,239 - 1,331

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

active site cd09272

1,847D

active site
1,847D, 1,889E, 1,924D, 1,973D
CDD
cd09272 (RNase_HI_RT_Ty1)

1,889E

active site
1,847D, 1,889E, 1,924D, 1,973D
CDD
cd09272 (RNase_HI_RT_Ty1)

1,924D

active site
1,847D, 1,889E, 1,924D, 1,973D
CDD
cd09272 (RNase_HI_RT_Ty1)

1,973D

active site
1,847D, 1,889E, 1,924D, 1,973D
CDD
cd09272 (RNase_HI_RT_Ty1)
RNA/DNA hybrid bin...

1,847D

RNA/DNA hybrid binding site
1,847D, 1,848A, 1,849N, 1,850L, 1,884S, 1,885S, 1,886A, 1,889E, 1,924D, 1,950M, 1,977K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,848A

RNA/DNA hybrid binding site
1,847D, 1,848A, 1,849N, 1,850L, 1,884S, 1,885S, 1,886A, 1,889E, 1,924D, 1,950M, 1,977K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,849N

RNA/DNA hybrid binding site
1,847D, 1,848A, 1,849N, 1,850L, 1,884S, 1,885S, 1,886A, 1,889E, 1,924D, 1,950M, 1,977K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,850L

RNA/DNA hybrid binding site
1,847D, 1,848A, 1,849N, 1,850L, 1,884S, 1,885S, 1,886A, 1,889E, 1,924D, 1,950M, 1,977K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,884S

RNA/DNA hybrid binding site
1,847D, 1,848A, 1,849N, 1,850L, 1,884S, 1,885S, 1,886A, 1,889E, 1,924D, 1,950M, 1,977K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,885S

RNA/DNA hybrid binding site
1,847D, 1,848A, 1,849N, 1,850L, 1,884S, 1,885S, 1,886A, 1,889E, 1,924D, 1,950M, 1,977K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,886A

RNA/DNA hybrid binding site
1,847D, 1,848A, 1,849N, 1,850L, 1,884S, 1,885S, 1,886A, 1,889E, 1,924D, 1,950M, 1,977K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,889E

RNA/DNA hybrid binding site
1,847D, 1,848A, 1,849N, 1,850L, 1,884S, 1,885S, 1,886A, 1,889E, 1,924D, 1,950M, 1,977K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,924D

RNA/DNA hybrid binding site
1,847D, 1,848A, 1,849N, 1,850L, 1,884S, 1,885S, 1,886A, 1,889E, 1,924D, 1,950M, 1,977K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,950M

RNA/DNA hybrid binding site
1,847D, 1,848A, 1,849N, 1,850L, 1,884S, 1,885S, 1,886A, 1,889E, 1,924D, 1,950M, 1,977K
CDD
cd09272 (RNase_HI_RT_Ty1)

1,977K

RNA/DNA hybrid binding site
1,847D, 1,848A, 1,849N, 1,850L, 1,884S, 1,885S, 1,886A, 1,889E, 1,924D, 1,950M, 1,977K
CDD
cd09272 (RNase_HI_RT_Ty1)

Protein sequence

>MCA_03184_1
MFQHTSFMKRKIENIDTPIKYLKGEQFKYFATDYDIYKISPNFKSRYRDYTSEINRFEEFYTKELKLIAKTTDNPITKFA
IDYANSITTTTETDSKQVRELKRLLVMTNEAHAIHHATTIEAYIHSQREIYLHYRGVLLSGVNNKSVLKKLTNSKPHLID
TRYREFNIGDIAEIESEPNYPRAQIVKPITNCSKTIFEYGSRDSDFNQIKAKLCRDYQEYNEAVKKNTKKPNTSGNNSNN
DGNKNTTNNNNSNTSNSNNDNNNNNNNNYNNNNNYNRNRGFNQNNRNQTRAANYVEQRSRQINWDNADSEIDLTNLSTPD
ISDVDGGVYNVGLATHKISNDRTGVKQTGRSINKDTNFTITPNMMFKVYDSDDLESETLIDSAASVAVINDKNLFYELEE
RQTVLDGPSGSGTVTSYGQGKVVYMLDNGEKPFLRSFNAYFVPDSHYNLIPEGFILKQLSIGGLGKSGEEESPRKCYNNK
DEYIYGFYSPRTLYTYFNGSFLVKLEKPNTNTTTEKNQKISHTSENVKDNHTPVNDYNTSDNTDNDGSDGEVEHQERGGE
ILFDTPDDDADFELDPDDANYSYGGNPLYNNDTSTLVFERRESPKTDSTNFEIKPTSQALVWHNTYNHAPTRQMKKLFEV
NNQNIPITKYDEELIANCPFCSVSKFTRNFKPEEHANRFENSAPLSTIHADITGYYPYPDLSGNRYALTIIDGYSNYVTT
ITIQTKDQATQKLIDWINKQENYFRGKTNYNVKRLQCDNGSEFTNNEFRQFCSEKGIEITFSPPDEPSRNGKVERFNRTI
EEKAQAACQKAGLPHEFYWSFAKIHASLVYNYVALDSNNKSPYELFCGKAPNTKGLLPFGCRIDVPRTGGDLHSPRGCLA
IYLGQGSNGRLRLYECPFNGRMATTGSGKPHPTEFPGRNMTFDKSILQTPLNERTYPRQSKTTNSYFSPTFVAGYMRNNA
EVDYFEESTFSGTDDEFYDNTFEGAEKAIEDEKIENLNRKYNNETYVNVSDDDTSENAEDESFSDSDQYGDCDDGGDETY
ETENDENGESSPNPDTTMTDAENFQQSNIGENQQNIINENINQPDTTRQTRNVHFEDPMDTDEDMQEESSVNSESEPSNE
SYEYTIPRNNSDAEESSTTSSTTYSSPTRQQRQRRDDGFMMSTNEPTNSNTTEEETHERHVQSSRITELPNDSTPMPVTP
SNNRNYNDNRFDTPSVVEQVATSTPFQRENQAIDQQLISRNSNQQLVNTNSERQISTSNNERQLSTTNSNRQLSTTNSER
QMSTTNSERQLSTTNSERQMSTTNSERQISMTNSERQVSTTNGERSIVPTNFQDRLAAIDEELNNRYFSNRPIEAPPERP
LLTNDDFQQQENNQMVSTNSNSNPYAEDTFMTETDGTIINYEDGVTITYETDTPEAPEVPVVEAPHRPVTRSMTRRLREQ
AGNDEDIRFINHVSSSTIYKNSKQETFQVVKSIEGNPDFIRVPCREVPKQDSEKWLEKMDGEMVALKVNKVYKLVKLPPG
FTPLRVRWVLVKKYDANGDLERYKARLVVRGYLQKEGINYGETFSPVARYDTVRLVFAIATKFNHEVVHMDFTNAFQFGE
IDYEIYVTQPPGYLAKGKEDHVLKLEKSLYGLKQAPLCWSRRLNKAISKLGYKATIKEPCLYVKEENGRISIICTYVDDL
LVSTPIKGEVDRIYQAFTDEGLVVKNLGFPSKFLGMRVTKKDDTIRLDLKTYIKDLVEFAGLQHANPEITPFKSSVKLEK
LKPEEAGKPIKDPKLFRSIIGKLIFASNTCRPDISTAVSILSQYLKEPREAHLTCAKTVVKYLHNTADFSLKFKNTEGAP
LVVGYADANLTNNADSKSRSGYIIKIYGNIVSWMSKKQTRAAQSSAESELYALNKAVKEMLYFRDLLGYELGLQLEGAST
IYEDNQPCIDMAKQPKINARTRYIRSDHMMIHECVERNEISVEHIGTKKMLADGFTKILPAPEFRKFYEDLDLVPDDYQR
RERNE

GO term prediction

Biological Process

GO:0015074 DNA integration

Molecular Function

GO:0003676 nucleic acid binding

Cellular Component