Protein

MCA_02638_1

Length
335 amino acids

Gene name: GET3

Description: ATPase GET3

Browser: contigB:1918193-1919284-

RNA-seq: read pairs 4086, FPKM 150.3, percentile rank 84.9% (100% = highest expression)

Protein function

Annotation:GET3ATPase GET3
KEGG:K01551arsA arsenite-transporting ATPase [EC:3.6.3.16]
EGGNOG:0PG12GET3ATPase required for the post-translational delivery of tail-anchored (TA) proteins to the endoplasmic reticulum. Recognizes and selectively binds the transmembrane domain of TA proteins in the cytosol. This complex then targets to the endoplasmic reticulum by membrane-bound receptors, where the tail- anchored protein is released for insertion. This process is regulated by ATP binding and hydrolysis. ATP binding drives the homodimer towards the closed dimer state, facilitating recognition of newly synthesized TA membrane proteins. ATP hydrolysis is required for insertion. Subsequently, the homodimer reverts towards the open dimer state, lowering its affinity for the membrane-bound receptor, and returning it to the cytosol to initiate a new round of targeting (By similarity)
SGD closest match:S000002258GET3ATPase GET3
CGD closest match:CAL0000195169GET3ATPase GET3

Protein alignments

%idAln lengthE-value
MIA_02868_181.60%3260.0MIA_02868_1
A0A0J9X687_GEOCN77.09%3236e-169ATPase GET3 OS=Geotrichum candidum GN=GET3 PE=3 SV=1
A0A167EBW2_9ASCO73.09%3278e-169ATPase GET3 OS=Sugiyamaella lignohabitans GN=GET3 PE=3 SV=1
UniRef50_A0A167EBW273.09%3272e-165ATPase GET3 n=8 Tax=Eukaryota TaxID=2759 RepID=A0A167EBW2_9ASCO
GET3_YARLI74.53%3224e-165ATPase GET3 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=GET3 PE=3 SV=1
A0A1E3PSQ6_9ASCO74.77%3335e-164ATPase GET3 OS=Nadsonia fulvescens var. elongata DSM 6958 GN=GET3 PE=3 SV=1
A0A060SYF0_BLAAD71.82%3302e-162ATPase GET3 OS=Blastobotrys adeninivorans GN=GET3 PE=3 SV=1
A0A1E4TH41_9ASCO64.24%3165e-133ATPase GET3 OS=Tortispora caseinolytica NRRL Y-17796 GN=GET3 PE=3 SV=1
GET3_CANAL57.78%3343e-119ATPase GET3 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=GET3 PE=3 SV=1
GET3_YEAST52.34%3424e-112ATPase GET3 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=GET3 PE=1 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.1762

Protein family membership

Domains and repeats

  1. Domain
1 50 100 150 200 250 300 335

Detailed signature matches

  1. Family IPR016300
    Arsenical pump ATPase, ArsA/GET3
    1. cd02035 (ArsA)
    2. TIGR00345
  2. Family IPR027542
    Arsenical pump ATPase, ArsA/GET3, eukaryotic
    1. MF_03112 (Asna1_Get3)
  3. Domain IPR027417
    P-loop containing nucleoside triphosphate hydrolase
    1. SSF52540 (P-loop co...)
  4. Domain IPR025723
    Anion-transporting ATPase-like domain
    1. PF02374 (ArsA_ATPase)
Unintegrated signatures no IPR
Unintegrated signatures
  1. G3DSA:3.40.50...

Residue annotation

  1. Switch I cd02035
  2. Nucleotide binding...
  3. DTAP/Switch II cd0...

Protein sequence

>MCA_02638_1
MADEVYEPSLQNIIDQKSLRWIFVGGKGGVGKTTTSCSLAVQLAQNRESVLLISTDPAHNLSDAFDQKFGKDPKPVEGIP
NLSCMEIDPSGSLQDMIGPNSNSGTGDAKDAVNSLMSDIAYSIPGIDEAMSFAEVMKQVKSTSFTTIVFDTAPTGHTLRF
LSFPSVLEKALAKIADLSNMYGPMLSGLMGSNGPNTSEMMAKLTETRETISEVNSQFKNPDLTTFVCVCIPEFLSLYETE
RMIQELTSFEIDTHCIVVNQLLFPKKGCECEQCLARFKIQQKYLEQILELYEDFHIVQLPQLTSEVRGVKALKEFSKMLI
EPYHPDNVMNETIQN

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0005524 ATP binding
GO:0016887 ATPase activity

Cellular Component

None predicted.