MCA_02615_1

Protein

Length

1,446 amino acids

Gene name: RAD5

Description: DNA repair protein RAD5

Browser: contigB:1826227-1830568-

RNA-seq: read pairs 1327, FPKM 11.3, percentile rank 28.1% (100% = highest expression)

Protein function

Annotation:		RAD5	DNA repair protein RAD5
KEGG:	K15505	RAD5	DNA repair protein RAD5 [EC:3.6.4.-]
EGGNOG:	0QDFS	RAD5	helicase, member of the UBC2 RAD6 epistasis group. Functions with DNA repair protein RAD18 in error-free postreplication DNA repair. Involved in the maintenance of wild- type rates of instability of simple repetitive sequences such as poly(GT) repeats. Seems to be involved in maintaining a balance which acts in favor of error-prone non-homologous joining during DNA double-strand breaks repairs (By similarity)
SGD closest match:	S000004022	RAD5	DNA repair protein RAD5
CGD closest match:	CAL0000193075	RAD5	DNA repair protein RAD5

Protein alignments

	%id	Aln length	E-value
MIA_00121_1	41.50%	1465	0.0	MIA_00121_1
A0A0J9X9F4_GEOCN	38.15%	1355	0.0	Similar to Saccharomyces cerevisiae YLR032W RAD5 DNA helicase proposed to promote replication fork regression during postreplication repair by template switching OS=Geotrichum candidum GN=BN980_GECA06s01077g PE=4 SV=1
UniRef50_A0A0J9X9F4	38.15%	1355	0.0	Similar to Saccharomyces cerevisiae YLR032W RAD5 DNA helicase proposed to promote replication fork regression during postreplication repair by template switching n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9X9F4_GEOCN
A0A060T5S8_BLAAD	41.65%	934	0.0	ARAD1B09856p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1B09856g PE=4 SV=1
A0A167FAC0_9ASCO	34.85%	1389	0.0	DNA helicase RAD5 OS=Sugiyamaella lignohabitans GN=RAD5 PE=4 SV=1
A0A1E3PCQ5_9ASCO	36.08%	1322	0.0	Uncharacterized protein (Fragment) OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_39087 PE=4 SV=1
RAD5_YEAST	31.05%	1224	1e-142	DNA repair protein RAD5 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RAD5 PE=1 SV=1
RAD5_CANAL	39.10%	532	8e-98	DNA repair protein RAD5 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=RAD5 PE=3 SV=2
A0A1E4TLD5_9ASCO	29.00%	807	1e-94	Uncharacterized protein (Fragment) OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_15482 PE=4 SV=1
RAD5_YARLI	37.28%	523	5e-86	DNA repair protein RAD5 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=RAD5 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0280

Protein family membership

None predicted.

Domains and repeats

Domain
213 - 332
HIRAN domain (IPR014905)
516 - 859
Helicase superfamily 1/2, ATP-binding domain (IPR014001)
1,067 - 1,183
Zinc finger, RING/FYVE/PHD-type (IPR013083)
Zinc finger, RING-type (IPR001841)
1,237 - 1,425
Helicase, C-terminal (IPR001650)
Domain
500 - 537
P-loop containing nucleoside triphosphate hydrolase (IPR027417)
604 - 727
P-loop containing nucleoside triphosphate hydrolase (IPR027417)
780 - 886
P-loop containing nucleoside triphosphate hydrolase (IPR027417)
931 - 1,002
P-loop containing nucleoside triphosphate hydrolase (IPR027417)
1,191 - 1,215
P-loop containing nucleoside triphosphate hydrolase (IPR027417)
1,249 - 1,296
P-loop containing nucleoside triphosphate hydrolase (IPR027417)
1,324 - 1,412
P-loop containing nucleoside triphosphate hydrolase (IPR027417)
Domain
523 - 899
SNF2-related, N-terminal domain (IPR000330)

1 200 400 600 800 1000 1200 1446

Detailed signature matches

IPR014905

HIRAN domain
1. PF08797 (HIRAN)
  
  213 - 328
  
  Pfam
  PF08797 (HIRAN)
2. SM00910 (HIRAN_2)
  
  213 - 332
  
  SMART
  SM00910 (HIRAN_2)
IPR027417

P-loop containing nucleoside triphosphate hydrolase
1. SSF52540 (P-loop co...)
  
  500 - 537
  
  SUPERFAMILY
  SSF52540 (P-loop containing nucleoside triphosphate hydrolases)
  
  604 - 727
  
  SUPERFAMILY
  SSF52540 (P-loop containing nucleoside triphosphate hydrolases)
  
  780 - 886
  
  SUPERFAMILY
  SSF52540 (P-loop containing nucleoside triphosphate hydrolases)
  
  931 - 1,002
  
  SUPERFAMILY
  SSF52540 (P-loop containing nucleoside triphosphate hydrolases)
  
  1,191 - 1,215
  
  SUPERFAMILY
  SSF52540 (P-loop containing nucleoside triphosphate hydrolases)
  
  1,249 - 1,296
  
  SUPERFAMILY
  SSF52540 (P-loop containing nucleoside triphosphate hydrolases)
  
  1,324 - 1,412
  
  SUPERFAMILY
  SSF52540 (P-loop containing nucleoside triphosphate hydrolases)
IPR014001

Helicase superfamily 1/2, ATP-binding domain
1. PS51192 (HELICASE_A...)
  
  599 - 847
  
  PROSITE profiles
  PS51192 (HELICASE_ATP_BIND_1)
2. SM00487 (ultradead3)
  
  516 - 859
  
  SMART
  SM00487 (ultradead3)
IPR000330

SNF2-related, N-terminal domain
1. PF00176 (SNF2_N)
  
  523 - 899
  
  Pfam
  PF00176 (SNF2_N)
IPR013083

Zinc finger, RING/FYVE/PHD-type
1. G3DSA:3.30.40.10
  
  1,067 - 1,183
  
  GENE3D
  G3DSA:3.30.40.10 (G3DSA:3.30.40.10)
IPR001841

Zinc finger, RING-type
1. SM00184 (ring_2)
  
  1,090 - 1,142
  
  SMART
  SM00184 (ring_2)
2. PS50089 (ZF_RING_2)
  
  1,090 - 1,143
  
  PROSITE profiles
  PS50089 (ZF_RING_2)
IPR001650

Helicase, C-terminal
1. PS51194 (HELICASE_CTER)
  
  1,237 - 1,425
  
  PROSITE profiles
  PS51194 (HELICASE_CTER)
2. SM00490 (helicmild6)
  
  1,262 - 1,373
  
  SMART
  SM00490 (helicmild6)
3. PF00271 (Helicase_C)
  
  1,245 - 1,373
  
  Pfam
  PF00271 (Helicase_C)
IPR017907

Zinc finger, RING-type, conserved site
1. PS00518 (ZF_RING_1)
  
  1,108 - 1,117
  
  PROSITE patterns
  PS00518 (ZF_RING_1)

no IPR

Unintegrated signatures

G3DSA:3.40.50...

521 - 887

GENE3D
G3DSA:3.40.50.10810 (G3DSA:3.40.50.10810)
G3DSA:3.40.50...

1,184 - 1,411

GENE3D
G3DSA:3.40.50.300 (G3DSA:3.40.50.300)
SSF57850 (RING/U-box)

1,079 - 1,149

SUPERFAMILY
SSF57850 (RING/U-box)
cd00046 (DEXDc)

606 - 829

CDD
cd00046 (DEXDc)
cd00079 (HELICc)

1,227 - 1,381

CDD
cd00079 (HELICc)
cd16449 (RING-HC)

1,090 - 1,142

CDD
cd16449 (RING-HC)
mobidb-lite (disord...)

1,162 - 1,182

MobiDB Lite
mobidb-lite (disorder_prediction)

26 - 69

MobiDB Lite
mobidb-lite (disorder_prediction)

1,302 - 1,322

MobiDB Lite
mobidb-lite (disorder_prediction)

1,413 - 1,438

MobiDB Lite
mobidb-lite (disorder_prediction)

895 - 925

MobiDB Lite
mobidb-lite (disorder_prediction)

165 - 206

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

ATP binding site c...

615G

ATP binding site
615G, 616L, 617G, 618K, 619T
CDD
cd00046 (DEXDc)

616L

ATP binding site
615G, 616L, 617G, 618K, 619T
CDD
cd00046 (DEXDc)

617G

ATP binding site
615G, 616L, 617G, 618K, 619T
CDD
cd00046 (DEXDc)

618K

ATP binding site
615G, 616L, 617G, 618K, 619T
CDD
cd00046 (DEXDc)

619T

ATP binding site
615G, 616L, 617G, 618K, 619T
CDD
cd00046 (DEXDc)
putative Mg++ bind...

798D

putative Mg++ binding site
798D, 799E, 800G, 801H
CDD
cd00046 (DEXDc)

799E

putative Mg++ binding site
798D, 799E, 800G, 801H
CDD
cd00046 (DEXDc)

800G

putative Mg++ binding site
798D, 799E, 800G, 801H
CDD
cd00046 (DEXDc)

801H

putative Mg++ binding site
798D, 799E, 800G, 801H
CDD
cd00046 (DEXDc)
Zn binding site cd...

1,090C

Zn binding site
1,090C, 1,093C, 1,108C, 1,110H, 1,113C, 1,116C, 1,139C, 1,142C
CDD
cd16449 (RING-HC)

1,093C

Zn binding site
1,090C, 1,093C, 1,108C, 1,110H, 1,113C, 1,116C, 1,139C, 1,142C
CDD
cd16449 (RING-HC)

1,108C

Zn binding site
1,090C, 1,093C, 1,108C, 1,110H, 1,113C, 1,116C, 1,139C, 1,142C
CDD
cd16449 (RING-HC)

1,110H

Zn binding site
1,090C, 1,093C, 1,108C, 1,110H, 1,113C, 1,116C, 1,139C, 1,142C
CDD
cd16449 (RING-HC)

1,113C

Zn binding site
1,090C, 1,093C, 1,108C, 1,110H, 1,113C, 1,116C, 1,139C, 1,142C
CDD
cd16449 (RING-HC)

1,116C

Zn binding site
1,090C, 1,093C, 1,108C, 1,110H, 1,113C, 1,116C, 1,139C, 1,142C
CDD
cd16449 (RING-HC)

1,139C

Zn binding site
1,090C, 1,093C, 1,108C, 1,110H, 1,113C, 1,116C, 1,139C, 1,142C
CDD
cd16449 (RING-HC)

1,142C

Zn binding site
1,090C, 1,093C, 1,108C, 1,110H, 1,113C, 1,116C, 1,139C, 1,142C
CDD
cd16449 (RING-HC)
nucleotide binding...

1,256Q

nucleotide binding region
1,256Q, 1,257F, 1,258T, 1,259Q, 1,279D, 1,280G, 1,335L, 1,336K, 1,337S
CDD
cd00079 (HELICc)

1,257F

nucleotide binding region
1,256Q, 1,257F, 1,258T, 1,259Q, 1,279D, 1,280G, 1,335L, 1,336K, 1,337S
CDD
cd00079 (HELICc)

1,258T

nucleotide binding region
1,256Q, 1,257F, 1,258T, 1,259Q, 1,279D, 1,280G, 1,335L, 1,336K, 1,337S
CDD
cd00079 (HELICc)

1,259Q

nucleotide binding region
1,256Q, 1,257F, 1,258T, 1,259Q, 1,279D, 1,280G, 1,335L, 1,336K, 1,337S
CDD
cd00079 (HELICc)

1,279D

nucleotide binding region
1,256Q, 1,257F, 1,258T, 1,259Q, 1,279D, 1,280G, 1,335L, 1,336K, 1,337S
CDD
cd00079 (HELICc)

1,280G

nucleotide binding region
1,256Q, 1,257F, 1,258T, 1,259Q, 1,279D, 1,280G, 1,335L, 1,336K, 1,337S
CDD
cd00079 (HELICc)

1,335L

nucleotide binding region
1,256Q, 1,257F, 1,258T, 1,259Q, 1,279D, 1,280G, 1,335L, 1,336K, 1,337S
CDD
cd00079 (HELICc)

1,336K

nucleotide binding region
1,256Q, 1,257F, 1,258T, 1,259Q, 1,279D, 1,280G, 1,335L, 1,336K, 1,337S
CDD
cd00079 (HELICc)

1,337S

nucleotide binding region
1,256Q, 1,257F, 1,258T, 1,259Q, 1,279D, 1,280G, 1,335L, 1,336K, 1,337S
CDD
cd00079 (HELICc)
ATP-binding site c...

1,343N

ATP-binding site
1,343N, 1,364Q, 1,368R, 1,371R
CDD
cd00079 (HELICc)

1,364Q

ATP-binding site
1,343N, 1,364Q, 1,368R, 1,371R
CDD
cd00079 (HELICc)

1,368R

ATP-binding site
1,343N, 1,364Q, 1,368R, 1,371R
CDD
cd00079 (HELICc)

1,371R

ATP-binding site
1,343N, 1,364Q, 1,368R, 1,371R
CDD
cd00079 (HELICc)

Protein sequence

>MCA_02615_1
MTDQNQDISVAEPVTKRVKFFHKYPSDWQGSQDVSKEATPSPEEQEAQSVQETSEHETEALQDPEPLSNQHAETVIAVNT
TQETISQINRNLNQQYSEFRKQIESVVGPITDERFHEARDRSNGDLNIAINIIFDEPAATKKTSQKQKALDTVSKSQSII
VESNSTQSRLAFSQPSSNSNSNNATPTETAAPETRSMSESQSGSENTWKRKYIGSLQAEIMVTRSGRNLISYQDPLYIIG
GMSYTNNPAKAPAFNTNKDPNSINHISAYKTDSFVRVCNKNNVEIGRFFGPMAKIVGVLLDTGTCQFEATCIFANETLKI
GDQFYIQLDCYLLKSGFDAYDKKVLGKDSGDSVVQKYHLSLLPRSKQKAYASESDSDDVSSRSSFFNNMKESKEELMLKL
KQKSLVGLFQKLGLKQVLHANSNNKSAAFPSALSETQGDNLLDQISKAAEISSTQAQDAKEDDEDENAIETETGFANGSN
SDFKEIELDQLDKVYRRCGEESQDFELSEIEPPKSFNLNLRAYQKRGLNWMLRRESEDISMTKSIDSNGNEETLDEPMHP
LWQEYEFPLDPNDKSQTEENGRQTFYANLYSGDLSLIFPRQKKAVKGGILADEMGLGKTISIMALVHSNKYNVPPPLSDP
NAHIPENSPQSTSDFAKYTTLIVAPMSLLSQWESEAYKASKPGTMRVLVYYGATAHSVNLRKLLCGPDAKSTAPHIVITS
YGTIASEHSTLVQFKNKQHLQRLATTRQQQSLFENGDQQANTQGRKLILGTEEMEWEDHLDLGLFGVFGINFYRIVLDEG
HMIKNRATRSAKSCYALRGERRWVLTGTPIVNRLEDLFSLIKFLKAEPWDNFSFWKTFITVPFVNKKFDRALGVIQKVVE
PILLRRTKDMKEKVLIPNEEDESNANQGDGLKDSADDSTNNTKNGTIIERPLVELPSKEVIISRINLSTEEREIYNLMFA
KAKGTYENSVSSGSVMKKYTTILAQILRLRQACCHPALVTRALMATRKLAEGTIINSSKKRSKDSSSTTLNEFVDDGFDN
DTVAALVAKFNNADNSKDWNGAEIDIDTSNQEKFGVEMLHKIEHEEAIECPICACESIPLDQRAVPTCWHVFCLPCLLEL
FEYNNNKTNSDDEVDFAKCPVCTASISKDKIYLIRRNEVDEESDYEDEDDNQERIKSSNNHSSSVRIKLKHYNPFTQSSK
IQFLVDKLKETRSYNRKLMKWKKQQGKLYNNDDNQEKINEDELFTKKPIKTVVFSQFTQFLDIIQQELKSEGFRYVRFDG
TLSQQERAKVLAQFTGEYYNNNNNNSNIQPYEQRGRNSRKNQRAKKSIDILLISLKSGGVGLNLVCAQQVFMMDPWWSFA
VEAQAIDRIHRMGQSEQVKVYRLIVSDSVEEKMLKIQEQKKLLALSSLENTSGYNNSNNNTGDGSNKNKLGGGESEKARE
FRMVFE

GO term prediction

Biological Process

None predicted.

Molecular Function

GO:0003676 nucleic acid binding
GO:0005524 ATP binding
GO:0008270 zinc ion binding
GO:0016818 hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides

Cellular Component

None predicted.

Filter view on

Entry type

Status

Per-residue features

MCA_02615_1

Protein function

Protein alignments

Mitochondrial localization by mitoprotII

Protein family membership

Domains and repeats

Detailed signature matches

Residue annotation

Protein sequence

GO term prediction

Biological Process

Molecular Function

Cellular Component