Protein

MCA_02462_1

Length
1,027 amino acids

Gene name: LIG4

Description: DNA ligase 4; DNA ligase required for nonhomologous end-joining (NHEJ)

Browser: contigB:1377008-1380092+

RNA-seq: read pairs 603, FPKM 7.2, percentile rank 21.0% (100% = highest expression)

Protein function

Annotation:LIG4DNA ligase 4; DNA ligase required for nonhomologous end-joining (NHEJ)
KEGG:K10777LIG4 DNA ligase 4 [EC:6.5.1.1]
EGGNOG:0PFMHLIG4dna ligase
SGD closest match:S000005531DNL4DNA ligase 4
CGD closest match:CAL0000187710LIG4DNA ligase 4

Protein alignments

%idAln lengthE-value
MIA_05818_147.08%10450.0MIA_05818_1
A0A0J9XHB9_GEOCN33.37%10342e-153DNA ligase OS=Geotrichum candidum GN=BN980_GECA18s00098g PE=3 SV=1
UniRef50_A0A0J9XHB933.37%10345e-150DNA ligase n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9XHB9_GEOCN
DNLI4_YARLI30.97%10433e-140DNA ligase 4 OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=LIG4 PE=3 SV=1
A0A1E3PIA4_9ASCO32.91%8722e-138DNA ligase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_66750 PE=3 SV=1
A0A060SZN6_BLAAD31.17%9886e-135ARAD1C02090p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C02090g PE=3 SV=1
A0A1E4TJ77_9ASCO31.61%8132e-111DNA ligase (Fragment) OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_15943 PE=3 SV=1
DNLI4_CANAL24.63%8893e-77DNA ligase 4 OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=LIG4 PE=2 SV=4
DNLI4_YEAST27.61%8731e-71DNA ligase 4 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=DNL4 PE=1 SV=1
A0A167CYF3_9ASCO31.67%4801e-66DNA ligase (ATP) DNL4 OS=Sugiyamaella lignohabitans GN=DNL4 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0250

Protein family membership

None predicted.

Domains and repeats

  1. Domain
  2. Domain
1 100 200 300 400 500 600 700 800 900 1027

Detailed signature matches

  1. Domain IPR012308
    DNA ligase, ATP-dependent, N-terminal
    1. PF04675 (DNA_ligase...)
    2. G3DSA:1.10.32...
  2. Domain IPR012310
    DNA ligase, ATP-dependent, central
    1. PS50160 (DNA_LIGASE_A3)
    2. PF01068 (DNA_ligase...)
  3. Domain IPR012340
    Nucleic acid-binding, OB-fold
    1. SSF50249 (Nucleic a...)
  4. Domain IPR012309
    DNA ligase, ATP-dependent, C-terminal
    1. PF04679 (DNA_ligase...)
  5. Domain IPR001357
    BRCT domain
    1. SSF52113 (BRCT domain)
    2. G3DSA:3.40.50...
    3. PS50172 (BRCT)
Unintegrated signatures no IPR
Unintegrated signatures
  1. Coil
  2. G3DSA:2.40.50...
  3. G3DSA:3.30.14...
  4. SSF56091 (DNA ligas...)
  5. cd07903 (Adenylatio...)
  6. cd07968 (OBF_DNA_li...)

Residue annotation

  1. active site cd07903
  2. DNA binding site c...
  3. DNA binding site c...

Protein sequence

>MCA_02462_1
MSTICSDDYPPEPSNFGSMPFRLFVKFLDRLESPDTTPTHRKEIVKEIVSLWRQKCGKNIFPCFRLLLPESDSARIFRLK
EKKLALEILQILHIDRKSEDGQAMLNWKGGYMAGTGDFPRRCRENILKRHGKKEYSNLNLDDINELLDDLVRLHTQKGGQ
KKVLEMMIKKLNAHEIYWMIKIIIKSMKIRMTEKSILDCWHPDAIKSFNVNSDLRRVCWELLDENSVVNTVDIQPFLCYK
PQMANFAMKDYSELLSKINSPEFYIEEKIDGERLQLHMVNDEFRYFSRKGNDNTEKYGKNYSSGSFTPLIKNFLHPNVRS
IVLDGEIVSWNEVECIVEGFSRVKGAINMIRNLESAVGIKKSFHDDEYNDTDKPDPAVKAPSKWRSAIEATDNNFHRHPF
YIVYDILYMNGVSFLSYPLKERRRVLETVLKPVNHVIEIIKYTKGSTKDDIDKRFMDVMEKDGEGLVIKNPDSAYRLNSR
NNDWVKVKPEYISNLGENLDLLIIGGYYGNGSRAGGLSSFLCGVRYSLLEQEDEHIDDDNTSFIPPFYSFCKVGGGFANE
EYEIIRSIFKDKTVPFTSNDRLPPCLDFGNQAPDVWIHPKDSIVIQIKAGQITVSDSYKAGMTLRFPRFTGFRYDKNWKN
ATSLNQLKQIRADIEAKRAKQQHELQKRKSGNAGKRSIKRVKLNIVSSSREYTSNEDLNHTASSAASSSLFKGKRFYVLT
SQRNPAYKSVKQLEILINTHSGSTIAFYPSISDTTEVPIYVIADIYTVGVSSFIKSHPEAVIVRPKWLWDSVQDKKIRPL
NPEYILTASEEEIYKAKCNVDRYGDNYYEASSVESLKPILSRMTPYTTRLNLLVPSKDGSTCNQLNFHKLLGAFSEMFQD
HFHNDMIPLNMLFASCGTIYFDFEQQSQAYILDIMKENSTFRQKESNYDPIAPTVTSLKAEAELDLVKARFYTKFGGAVI
ETDPSNPNIDIMVFSPFDTTRVRKLKEINSKRRTDERFAYCVDVSWIFASWRERTLLSEDLYTISDI

GO term prediction

Biological Process

GO:0006281 DNA repair
GO:0006310 DNA recombination

Molecular Function

GO:0003677 DNA binding
GO:0003910 DNA ligase (ATP) activity
GO:0005524 ATP binding

Cellular Component

None predicted.