MCA_02448_1

Protein

Length

382 amino acids

Description: Putative cysteine synthase

RNA-seq: read pairs 7369, FPKM 237.7, percentile rank 90.0% (100% = highest expression)

Protein function

Annotation:			Putative cysteine synthase
KEGG:	K01738	cysK	cysteine synthase A [EC:2.5.1.47]
EGGNOG:	0PJXH	FG07287.1	cysteine synthase
SGD closest match:	S000003244	YGR012W	Putative cysteine synthase
CGD closest match:	CAL0000173937	orf19.7152	Uncharacterized protein

Protein alignments

	%id	Aln length	E-value
MIA_04891_1	79.44%	360	0.0	MIA_04891_1
A0A0J9XHP3_GEOCN	79.04%	353	0.0	Similar to Saccharomyces cerevisiae YGR012W Putative cysteine synthase OS=Geotrichum candidum GN=BN980_GECA18s02320g PE=4 SV=1
A0A167E8S3_9ASCO	79.04%	353	0.0	Putative cysteine synthase OS=Sugiyamaella lignohabitans GN=AWJ20_4728 PE=4 SV=1
A0A060SY14_BLAAD	76.03%	363	0.0	ARAD1A17028p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1A17028g PE=4 SV=1
UniRef50_A0A060SY14	76.03%	363	0.0	ARAD1A17028p n=47 Tax=Eukaryota TaxID=2759 RepID=A0A060SY14_BLAAD
A0A1D8PRJ1_CANAL	69.70%	363	4e-176	Uncharacterized protein OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=orf19.7152 PE=4 SV=1
Q6C1R7_YARLI	70.80%	363	1e-175	YALI0F14047p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_F14047g PE=4 SV=1
A0A1E4TB08_9ASCO	67.45%	341	4e-159	Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_131213 PE=4 SV=1
A0A1E3PGD1_9ASCO	63.74%	342	4e-147	Cysteine synthase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_52605 PE=4 SV=1
CYSKL_YEAST	38.78%	361	8e-65	Putative cysteine synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=YGR012W PE=1 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.9752
Predicted cleavage: 32

Protein family membership

None predicted.

Domains and repeats

Domain
46 - 367
Tryptophan synthase beta subunit-like PLP-dependent enzyme (IPR001926)

1 50 100 150 200 250 300 350 382

Detailed signature matches

IPR001926

Tryptophan synthase beta subunit-like PLP-dependent enzyme
1. SSF53686 (Tryptopha...)
  
  46 - 367
  
  SUPERFAMILY
  SSF53686 (Tryptophan synthase beta subunit-like PLP-dependent enzymes)
2. PF00291 (PALP)
  
  47 - 347
  
  Pfam
  PF00291 (PALP)
IPR001216

Cysteine synthase/cystathionine beta-synthase, pyridoxal-phosphate attachment site
1. PS00901 (CYS_SYNTHASE)
  
  70 - 88
  
  PROSITE patterns
  PS00901 (CYS_SYNTHASE)

no IPR

Unintegrated signatures

G3DSA:3.40.50...

58 - 179

GENE3D
G3DSA:3.40.50.1100 (G3DSA:3.40.50.1100)

180 - 368

GENE3D
G3DSA:3.40.50.1100 (G3DSA:3.40.50.1100)
cd01561 (CBS_like)

49 - 354

CDD
cd01561 (CBS_like)

Residue annotation

dimer interface cd...

53L

dimer interface
53L, 54I, 55R, 68Y, 74M, 76P, 123H, 126R, 142Q, 143G, 146D, 147H, 150F, 151L, 208T, 209Q, 310L, 311N, 312E, 314G, 315L, 316F, 340T, 350D, 353V
CDD
cd01561 (CBS_like)

54I

dimer interface
53L, 54I, 55R, 68Y, 74M, 76P, 123H, 126R, 142Q, 143G, 146D, 147H, 150F, 151L, 208T, 209Q, 310L, 311N, 312E, 314G, 315L, 316F, 340T, 350D, 353V
CDD
cd01561 (CBS_like)

55R

dimer interface
53L, 54I, 55R, 68Y, 74M, 76P, 123H, 126R, 142Q, 143G, 146D, 147H, 150F, 151L, 208T, 209Q, 310L, 311N, 312E, 314G, 315L, 316F, 340T, 350D, 353V
CDD
cd01561 (CBS_like)

68Y

dimer interface
53L, 54I, 55R, 68Y, 74M, 76P, 123H, 126R, 142Q, 143G, 146D, 147H, 150F, 151L, 208T, 209Q, 310L, 311N, 312E, 314G, 315L, 316F, 340T, 350D, 353V
CDD
cd01561 (CBS_like)

74M

dimer interface
53L, 54I, 55R, 68Y, 74M, 76P, 123H, 126R, 142Q, 143G, 146D, 147H, 150F, 151L, 208T, 209Q, 310L, 311N, 312E, 314G, 315L, 316F, 340T, 350D, 353V
CDD
cd01561 (CBS_like)

76P

dimer interface
53L, 54I, 55R, 68Y, 74M, 76P, 123H, 126R, 142Q, 143G, 146D, 147H, 150F, 151L, 208T, 209Q, 310L, 311N, 312E, 314G, 315L, 316F, 340T, 350D, 353V
CDD
cd01561 (CBS_like)

123H

dimer interface
53L, 54I, 55R, 68Y, 74M, 76P, 123H, 126R, 142Q, 143G, 146D, 147H, 150F, 151L, 208T, 209Q, 310L, 311N, 312E, 314G, 315L, 316F, 340T, 350D, 353V
CDD
cd01561 (CBS_like)

126R

dimer interface
53L, 54I, 55R, 68Y, 74M, 76P, 123H, 126R, 142Q, 143G, 146D, 147H, 150F, 151L, 208T, 209Q, 310L, 311N, 312E, 314G, 315L, 316F, 340T, 350D, 353V
CDD
cd01561 (CBS_like)

142Q

dimer interface
53L, 54I, 55R, 68Y, 74M, 76P, 123H, 126R, 142Q, 143G, 146D, 147H, 150F, 151L, 208T, 209Q, 310L, 311N, 312E, 314G, 315L, 316F, 340T, 350D, 353V
CDD
cd01561 (CBS_like)

143G

dimer interface
53L, 54I, 55R, 68Y, 74M, 76P, 123H, 126R, 142Q, 143G, 146D, 147H, 150F, 151L, 208T, 209Q, 310L, 311N, 312E, 314G, 315L, 316F, 340T, 350D, 353V
CDD
cd01561 (CBS_like)

146D

dimer interface
53L, 54I, 55R, 68Y, 74M, 76P, 123H, 126R, 142Q, 143G, 146D, 147H, 150F, 151L, 208T, 209Q, 310L, 311N, 312E, 314G, 315L, 316F, 340T, 350D, 353V
CDD
cd01561 (CBS_like)

147H

dimer interface
53L, 54I, 55R, 68Y, 74M, 76P, 123H, 126R, 142Q, 143G, 146D, 147H, 150F, 151L, 208T, 209Q, 310L, 311N, 312E, 314G, 315L, 316F, 340T, 350D, 353V
CDD
cd01561 (CBS_like)

150F

dimer interface
53L, 54I, 55R, 68Y, 74M, 76P, 123H, 126R, 142Q, 143G, 146D, 147H, 150F, 151L, 208T, 209Q, 310L, 311N, 312E, 314G, 315L, 316F, 340T, 350D, 353V
CDD
cd01561 (CBS_like)

151L

dimer interface
53L, 54I, 55R, 68Y, 74M, 76P, 123H, 126R, 142Q, 143G, 146D, 147H, 150F, 151L, 208T, 209Q, 310L, 311N, 312E, 314G, 315L, 316F, 340T, 350D, 353V
CDD
cd01561 (CBS_like)

208T

dimer interface
53L, 54I, 55R, 68Y, 74M, 76P, 123H, 126R, 142Q, 143G, 146D, 147H, 150F, 151L, 208T, 209Q, 310L, 311N, 312E, 314G, 315L, 316F, 340T, 350D, 353V
CDD
cd01561 (CBS_like)

209Q

dimer interface
53L, 54I, 55R, 68Y, 74M, 76P, 123H, 126R, 142Q, 143G, 146D, 147H, 150F, 151L, 208T, 209Q, 310L, 311N, 312E, 314G, 315L, 316F, 340T, 350D, 353V
CDD
cd01561 (CBS_like)

310L

dimer interface
53L, 54I, 55R, 68Y, 74M, 76P, 123H, 126R, 142Q, 143G, 146D, 147H, 150F, 151L, 208T, 209Q, 310L, 311N, 312E, 314G, 315L, 316F, 340T, 350D, 353V
CDD
cd01561 (CBS_like)

311N

dimer interface
53L, 54I, 55R, 68Y, 74M, 76P, 123H, 126R, 142Q, 143G, 146D, 147H, 150F, 151L, 208T, 209Q, 310L, 311N, 312E, 314G, 315L, 316F, 340T, 350D, 353V
CDD
cd01561 (CBS_like)

312E

dimer interface
53L, 54I, 55R, 68Y, 74M, 76P, 123H, 126R, 142Q, 143G, 146D, 147H, 150F, 151L, 208T, 209Q, 310L, 311N, 312E, 314G, 315L, 316F, 340T, 350D, 353V
CDD
cd01561 (CBS_like)

314G

dimer interface
53L, 54I, 55R, 68Y, 74M, 76P, 123H, 126R, 142Q, 143G, 146D, 147H, 150F, 151L, 208T, 209Q, 310L, 311N, 312E, 314G, 315L, 316F, 340T, 350D, 353V
CDD
cd01561 (CBS_like)

315L

dimer interface
53L, 54I, 55R, 68Y, 74M, 76P, 123H, 126R, 142Q, 143G, 146D, 147H, 150F, 151L, 208T, 209Q, 310L, 311N, 312E, 314G, 315L, 316F, 340T, 350D, 353V
CDD
cd01561 (CBS_like)

316F

dimer interface
53L, 54I, 55R, 68Y, 74M, 76P, 123H, 126R, 142Q, 143G, 146D, 147H, 150F, 151L, 208T, 209Q, 310L, 311N, 312E, 314G, 315L, 316F, 340T, 350D, 353V
CDD
cd01561 (CBS_like)

340T

dimer interface
53L, 54I, 55R, 68Y, 74M, 76P, 123H, 126R, 142Q, 143G, 146D, 147H, 150F, 151L, 208T, 209Q, 310L, 311N, 312E, 314G, 315L, 316F, 340T, 350D, 353V
CDD
cd01561 (CBS_like)

350D

dimer interface
53L, 54I, 55R, 68Y, 74M, 76P, 123H, 126R, 142Q, 143G, 146D, 147H, 150F, 151L, 208T, 209Q, 310L, 311N, 312E, 314G, 315L, 316F, 340T, 350D, 353V
CDD
cd01561 (CBS_like)

353V

dimer interface
53L, 54I, 55R, 68Y, 74M, 76P, 123H, 126R, 142Q, 143G, 146D, 147H, 150F, 151L, 208T, 209Q, 310L, 311N, 312E, 314G, 315L, 316F, 340T, 350D, 353V
CDD
cd01561 (CBS_like)
catalytic residue ...

81K

catalytic residue
81K
CDD
cd01561 (CBS_like)
pyridoxal 5'-phosp...

81K

pyridoxal 5'-phosphate binding site
81K, 116N, 221T, 222G, 223T, 224G, 225G, 226S, 274G, 320S, 346C, 347D
CDD
cd01561 (CBS_like)

116N

pyridoxal 5'-phosphate binding site
81K, 116N, 221T, 222G, 223T, 224G, 225G, 226S, 274G, 320S, 346C, 347D
CDD
cd01561 (CBS_like)

221T

pyridoxal 5'-phosphate binding site
81K, 116N, 221T, 222G, 223T, 224G, 225G, 226S, 274G, 320S, 346C, 347D
CDD
cd01561 (CBS_like)

222G

pyridoxal 5'-phosphate binding site
81K, 116N, 221T, 222G, 223T, 224G, 225G, 226S, 274G, 320S, 346C, 347D
CDD
cd01561 (CBS_like)

223T

pyridoxal 5'-phosphate binding site
81K, 116N, 221T, 222G, 223T, 224G, 225G, 226S, 274G, 320S, 346C, 347D
CDD
cd01561 (CBS_like)

224G

pyridoxal 5'-phosphate binding site
81K, 116N, 221T, 222G, 223T, 224G, 225G, 226S, 274G, 320S, 346C, 347D
CDD
cd01561 (CBS_like)

225G

pyridoxal 5'-phosphate binding site
81K, 116N, 221T, 222G, 223T, 224G, 225G, 226S, 274G, 320S, 346C, 347D
CDD
cd01561 (CBS_like)

226S

pyridoxal 5'-phosphate binding site
81K, 116N, 221T, 222G, 223T, 224G, 225G, 226S, 274G, 320S, 346C, 347D
CDD
cd01561 (CBS_like)

274G

pyridoxal 5'-phosphate binding site
81K, 116N, 221T, 222G, 223T, 224G, 225G, 226S, 274G, 320S, 346C, 347D
CDD
cd01561 (CBS_like)

320S

pyridoxal 5'-phosphate binding site
81K, 116N, 221T, 222G, 223T, 224G, 225G, 226S, 274G, 320S, 346C, 347D
CDD
cd01561 (CBS_like)

346C

pyridoxal 5'-phosphate binding site
81K, 116N, 221T, 222G, 223T, 224G, 225G, 226S, 274G, 320S, 346C, 347D
CDD
cd01561 (CBS_like)

347D

pyridoxal 5'-phosphate binding site
81K, 116N, 221T, 222G, 223T, 224G, 225G, 226S, 274G, 320S, 346C, 347D
CDD
cd01561 (CBS_like)

Protein sequence

>MCA_02448_1
MLRQIRPLISKSSHSASLFSKRLLSSANEGFKMPFIPLVSESGFPGAVGNTPLIRLNKLSEETGANIYGKAEFMNPGGSV
KDRAALFIVQDAEKKGLIDPTDKENRPTIVEGTAGNTGIGLAHVCRARGYNCVIYMPDTQSQGKIDHLRFLGAEVHPVPA
VAFTDPNNYNHQAKRHAESLKNAVWTNQFDNTANRQAHIETTGPEIWTQTDGKIDGFTCATGTGGSLAGTSRYLKDISNG
KVQTWLADPPGSVLYQYVQNGKLERTGNGSITEGIGQGRLTDNLKPDVELMDGALHIPDADTINMVYRLLNEEGLFLGAS
SCLNVVAAYELAKILGPKSTVVTLLCDGADRYVDRLFSKKWLESKNLYESIPQELRKFIALP

GO term prediction

Biological Process

GO:0006535 cysteine biosynthetic process from serine

Molecular Function

None predicted.

Cellular Component

None predicted.

Filter view on

Entry type

Status

Per-residue features

MCA_02448_1

Protein function

Protein alignments

Mitochondrial localization by mitoprotII

Protein family membership

Domains and repeats

Detailed signature matches

Residue annotation

Protein sequence

GO term prediction

Biological Process

Molecular Function

Cellular Component