Protein

MCA_02323_1

Length
301 amino acids

Gene name: PUS5

Description: 21S rRNA pseudouridine(2819) synthase

Browser: contigB:943282-944188-

RNA-seq: read pairs 125, FPKM 5.1, percentile rank 17.6% (100% = highest expression)

Protein function

Annotation:PUS521S rRNA pseudouridine(2819) synthase
EGGNOG:0PN04PUS5responsible for the pseudouridine-2819 formation in mitochondrial 21S rRNA. May modulate the efficiency or the fidelity of the mitochondrial translation machinery
SGD closest match:S000004155PUS521S rRNA pseudouridine(2819) synthase

Protein alignments

%idAln lengthE-value
MIA_01132_142.00%3004e-72MIA_01132_1
A0A0J9X4J2_GEOCN32.80%3114e-37Similar to Saccharomyces cerevisiae YLR165C PUS5 Pseudouridine synthase OS=Geotrichum candidum GN=BN980_GECA02s05543g PE=4 SV=1
UniRef50_A0A0J9X4J232.80%3118e-34Similar to Saccharomyces cerevisiae YLR165C PUS5 Pseudouridine synthase n=1 Tax=Geotrichum candidum TaxID=1173061 RepID=A0A0J9X4J2_GEOCN
A0A1E3PHZ0_9ASCO34.28%2832e-27Pseudouridine synthase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_51577 PE=4 SV=1
PUS5_YEAST31.05%2482e-2121S rRNA pseudouridine(2819) synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PUS5 PE=1 SV=1
A0A167CCR8_9ASCO30.08%1334e-07Bifunctional DRAP deaminase/tRNA pseudouridine synthase RIB2 OS=Sugiyamaella lignohabitans GN=RIB2 PE=4 SV=1
A0A1E4TM99_9ASCO31.62%1363e-06Pseudouridine synthase OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_22508 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.7750

Protein family membership

None predicted.

Domains and repeats

  1. Domain
1 50 100 150 200 250 301

Detailed signature matches

  1. Domain IPR020103
    Pseudouridine synthase, catalytic domain
    1. SSF55120 (Pseudouri...)
  2. Domain IPR006145
    Pseudouridine synthase, RsuA/RluB/C/D/E/F
    1. PF00849 (PseudoU_sy...)
Unintegrated signatures no IPR
Unintegrated signatures
  1. G3DSA:3.30.23...
  2. cd02869 (PseudoU_sy...)

Residue annotation

  1. active site cd02869

Protein sequence

>MCA_02323_1
MTALFRRSIDILTQTKHFIIVNKPPLCYSQPPDTSHVTEEMLFNGFNPEDTVLNLLERSYPELYKQQTSEAKKQQDQQFF
KPKTVQRLDYNVSGAMTLATSNQAATMFSRNLKYGGSKGWSIHKYYAAILTKEPSLRNSHIHMAEDIYSESGKILLKSGI
INHNVDKKPAFTKFWTLPPSDKTPYHLALFSPLTGRKHQIRIHASQVLKAPILGDTVYTLKNKKPSQESLNIDGIYGSAI
ALHSYRIEVNVGKTIHQAKAPFWRNIPEWEHFMNLETRLLPDYLYTDVKVDDSETRMVRNG

GO term prediction

Biological Process

GO:0001522 pseudouridine synthesis
GO:0009451 RNA modification

Molecular Function

GO:0003723 RNA binding
GO:0009982 pseudouridine synthase activity

Cellular Component

None predicted.