Protein

MCA_02165_2

Length
2,399 amino acids

Gene name: PRP8

Description: Pre-mRNA-splicing factor 8

Browser: contigB:436336-443536+

RNA-seq: read pairs 1429, FPKM 7.4, percentile rank 21.2% (100% = highest expression)

Protein function

Annotation:PRP8Pre-mRNA-splicing factor 8
KEGG:K12856PRPF8 pre-mRNA-processing factor 8
EGGNOG:0PHSRPRP8factor 8
SGD closest match:S000001208PRP8Pre-mRNA-splicing factor 8
CGD closest match:CAL0000198077PRP8U4/U6-U5 snRNP complex subunit

Protein alignments

%idAln lengthE-value
MIA_03971_188.83%23370.0MIA_03971_1
A0A0J9XBM7_GEOCN84.92%23080.0Similar to Saccharomyces cerevisiae YHR165C PRP8 Component of the U4/U6-U5 snRNP complex OS=Geotrichum candidum GN=BN980_GECA07s01220g PE=4 SV=1
A0A060T7Z1_BLAAD79.26%23390.0ARAD1D03102p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1D03102g PE=4 SV=1
A0A1E3PKA9_9ASCO76.83%23220.0PROCN-domain-containing protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_51141 PE=4 SV=1
Q6CCC5_YARLI71.66%23220.0YALI0C10648p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_C10648g PE=4 SV=2
UniRef50_Q6CCC571.66%23220.0YALI0C10648p n=25 Tax=cellular organisms TaxID=131567 RepID=Q6CCC5_YARLI
Q5A322_CANAL67.60%23550.0U4/U6-U5 snRNP complex subunit OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=PRP8 PE=4 SV=1
A0A1E4TB19_9ASCO68.04%23250.0Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_65636 PE=4 SV=1
PRP8_YEAST66.94%23140.0Pre-mRNA-splicing factor 8 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=PRP8 PE=1 SV=1
A0A167EEA5_9ASCO83.10%18050.0U4/U6-U5 snRNP complex component PRP8 OS=Sugiyamaella lignohabitans GN=PRP8 PE=4 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.8086
Predicted cleavage: 56

Protein family membership

None predicted.

Domains and repeats

  1. Domain
  2. Domain
1 500 1000 1500 2000 2399

Detailed signature matches

  1. Domain IPR012591
    PRO8NT domain
    1. PF08082 (PRO8NT)
    2. PD149576
  2. Domain IPR012592
    PROCN domain
    1. PF08083 (PROCN)
  3. Domain IPR019582
    RNA recognition motif, spliceosomal PrP8
    1. PF10598 (RRM_4)
  4. Domain IPR012337
    Ribonuclease H-like domain
    1. SSF53098 (Ribonucle...)
  5. Domain IPR019581
    Pre-mRNA-processing-splicing factor 8, U5-snRNA-binding
    1. PF10597 (U5_2-snRNA...)
  6. Domain IPR019580
    Pre-mRNA-processing-splicing factor 8, U6-snRNA-binding
    1. PF10596 (U6-snRNA_bdg)
  7. Domain IPR021983
    PRP8 domain IV core
    1. cd13838 (RNase_H_li...)
    2. PF12134 (PRP8_domainIV)
  8. Domain IPR000555
    JAB1/MPN/MOV34 metalloenzyme domain
    1. SM00232 (pad1_6)
    2. PF01398 (JAB)
  9. Domain IPR012984
    PROCT domain
    1. PF08084 (PROCT)
Unintegrated signatures no IPR
Unintegrated signatures
  1. G3DSA:3.40.14...
  2. cd08056 (MPN_PRP8)
  3. mobidb-lite (disord...)

Residue annotation

  1. active site cd13838

Protein sequence

>MCA_02165_2
MKRNPPPPPPRSRRPPPPPGRSSKPSLPPPPPSSSRALPKKASTAPPPPPSGPRAPPPPPPSNSTGLDPKQIEDKVNKLQ
ERKKKWIQLQKRRYGEQKTKSSYVHATKIEMPPEHLRKIINDHGDMSTRKLAADKRSYLGALKYMPHAVIKLLENMPQPW
EQVREVKVLYHITGAITFVKESPRVIEPIYTAQWASMWIAMRREKRNRRHFKRMRFPPFDDEEPPINWSENIEITEPPEA
IQLELNEDEDGAVYEWFYDHQPLIDDKDHVNGPSYRRWRLSLSEMSVLHRLARPIISDRTDRNYYYLFDKESFFTAKALN
SAIPGGPKFEPLYTDINPDDEDFSEFNAIDRLIFRRTIRTEYKVAYPHLYNSLPRSVHISWYHDPQTCFVYAEDPDIPPF
HFNTTYEPISTRHVSGYKSSIPSHEDEIFGEGNNENDSFTLPDDFEAFLEDETLYTENTAGALNLWWAPYPFNRRSGPTV
RAEDVALVKHWYLEHPDTSYPVKVRVSYQKLLKTYVLNALKTKPPKNQKKRNLLTSLGNTKFFQETTIDWVEAGLQLCRQ
GFTMLNLLIHRKGLTYLHLDYNFNLKPIKTLTTKERKRSRFGNAFHLMREILRIVKLIVDSHVQYRLGNVDAFQLADGIY
YILNHIGQLTGIYRYKYRVMHQIRSCKDLKHVIYYKFNVAIGKGPGAGFWQPAWRVWLFFLRGIIPLLERWLGNLLARQF
EGRHSKDIAKTITKQRVESYYDLELRAQVLHDILDMIPEGIKQSKSKTILQHLSEAWRCWKANIPWKVPGLPKPIEDIIL
RYIKAKADGWISVAHYNRERIRRGATVDKTVAKKNLGRLTRLWLKSEQERQFNFSKDGPYVSPEEAVAIYSTMVHWLESR
KFSPIPFPPLSYKHDTKLLVLALEGLKDSYSVKGRLNQSQREELALIEQAYDNPHECLSRIKRLLLTQRVFKEVGFDMMD
HYSHLNPVYEVDPLEKITDAYLDHYLWYEGDRRHIFPNWIKPSDSEVPPLLVYKWCQGINNLTDIWDVSEGQCNVLLQTT
LSSVAEKIDFTLLNRLLRLIVDSNIADYITAKNNVNLSYKDMNHTNQYGLIRGLQFSSFVFQYYGLVIDILLLGLQRASE
LAGPPNQPNDFLQFESEEVETRHPIRMYTRYIDKIHILFRFTSEEAKDLNQRFLTENPDPNFENIIGYNNKKCWPRDSRM
RLMRHDVNLGRAVFWEMRNRLPRSITTINWEDTFASVYSKDNPNLLFSMVGFEVRILPKCRIESDIQICDSVWNLTNEST
KERTAYAFLRVSEEEIARFNNRIRQILMSSGSTTFTKIVNKWNTALISLFTYYREAAVATEELLDTLVRCETKIQTRVKI
GLNSKMPSRFPPAVFYTPKELGGLGMISASHILIPASDLRWSKQTDTGITHFRAGMSHEEDQLIPNIFRYLTSWESEFID
SQRVWAEYAMKRQEALQQNRRLTLEDLEDSWDRGLPRINTLFQKDRHTLAYDKGHRIRTEFRQYFITRVNPFWWTSQRHD
GKLWNLNAYRTDVIQALGGIETILEHTLFKGTGFDNWEGLFWEKASGFEDSLKFKKLTNAQRSGLNQIPNRRFTLWWSPT
INRANVYVGFLVQLDLTGIFLHGKIPTLKISLIQIFRAHLWQKIHESVIFDLAAVLDQEMDTLQIESVQKESIHPRKSYK
MNSSTADLVLFSTYRWSVSKPSLLHDSKDIMDFATTNKFWIDVQLRYGDYDSHDISRYTRAKFLDYTSDGISVYPSSTGL
MIGIDLAYNMYDAYGNWFLGLKPLIGQAMNKIMKANPSLFVLRERIRKGLQLYQSQPQEAFLNSQNYSELFSRDIQFFVD
DSNVYRVVTHKTFEGNMATKPINGALFIFNPKTGQLYLKIIHTSVWAGQKRMIQLSKWKTAEEVAALVRALPREEQPKQI
IVTRKGMLDPLEVHMLDFPNISIRPSELQLPFSAAIKIEKLADQILKATEPQMLMYNLYDDWLIDNKTEPGTAFSRLVLI
LRALHVNNEKAKIILRPDRTVVTQEYHLWPSYTVEQWVNVENQLSDLILNDYGKKNNVDPASLTQQEIRDIILGQEIKAP
SLRRQEIARIEQQEQEQSQLTAVKTKTQNVHGDEMVVVSTSNYEQQSFQSKTEWRSRAISTTNLHLRAKHIYVASDDIQD
DSDHFTYIMPKNVLKKFIQISDLRAQVGGILYGTSPDDNTQVKEIKAIAMIPQLGGVQSVQLANNLPDPNDPLLEGLEPL
GWIHTQAQESPFLSAIDVTAQAKLMKQNANWRTNSITMTVSFTPGSVSLTAYAITPEGVEWGFKNKDIFSSNPEGFSTKY
GEKCQLLLSDRIRGFFLVPETKLWNFAFIGAGWSPNAKYDLTIDNPIPFYHELHRPIHFTTFTELEGDDIGVDREDVYA

GO term prediction

Biological Process

GO:0000398 mRNA splicing, via spliceosome

Molecular Function

GO:0003676 nucleic acid binding
GO:0003723 RNA binding
GO:0005515 protein binding
GO:0017070 U6 snRNA binding
GO:0030623 U5 snRNA binding

Cellular Component

None predicted.