Protein

MCA_01672_1

Length
603 amino acids

Gene name: LEU4

Description: 2-isopropylmalate synthase

Browser: contigA:5139212-5141024-

RNA-seq: read pairs 4149, FPKM 84.9, percentile rank 76.2% (100% = highest expression)

Protein function

Annotation:LEU42-isopropylmalate synthase
KEGG:K01649leuA 2-isopropylmalate synthase [EC:2.3.3.13]
EGGNOG:0PGJG2-isopropylmalate synthase
SGD closest match:S000005048LEU42-isopropylmalate synthase
CGD closest match:CAL0000201867LEU42Leu42p

Protein alignments

%idAln lengthE-value
MIA_04483_186.59%6040.0MIA_04483_1
A0A0J9YHA9_GEOCN75.04%5890.0Similar to Saccharomyces cerevisiae YNL104C LEU4 Alpha-isopropylmalate synthase (2-isopropylmalate synthase) OS=Geotrichum candidum GN=BN980_GECA01s01660g PE=3 SV=1
Q6CFF6_YARLI69.35%5840.0YALI0B07447p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_B07447g PE=3 SV=1
A0A167F8Y7_9ASCO71.67%5860.02-isopropylmalate synthase LEU4 OS=Sugiyamaella lignohabitans GN=LEU4 PE=3 SV=1
A0A060T5N2_BLAAD70.91%5810.0ARAD1B08338p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1B08338g PE=3 SV=1
A0A1E3PRT7_9ASCO69.74%5750.0Alpha-isopropylmalate synthase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_49972 PE=3 SV=1
A0A1D8PIF8_CANAL65.02%5860.0Leu42p OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=LEU42 PE=3 SV=1
A0A1E4TKN6_9ASCO63.65%5860.0Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_85004 PE=3 SV=1
UniRef50_Q5AB8663.70%5840.02-isopropylmalate synthase n=9 Tax=saccharomyceta TaxID=716545 RepID=Q5AB86_CANAL
LEU1_YEAST60.99%6050.02-isopropylmalate synthase OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=LEU4 PE=1 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0222
Predicted cleavage: 20

Protein family membership

Domains and repeats

  1. Domain
1 100 200 300 400 500 603

Detailed signature matches

  1. Family IPR005668
    2-isopropylmalate synthase
    1. TIGR00970
    2. MF_00572 (LeuA_type2)
  2. Domain IPR013785
    Aldolase-type TIM barrel
    1. G3DSA:3.20.20.70
  3. Domain IPR000891
    Pyruvate carboxyltransferase
    1. PF00682 (HMGL-like)
    2. PS50991 (PYR_CT)
  4. Domain IPR013709
    2-isopropylmalate synthase LeuA, allosteric (dimerisation) domain
    1. SSF110921 (2-isopro...)
    2. PF08502 (LeuA_dimer)
    3. SM00917 (LeuA_dimer_2)
  5. SITE IPR002034
    Alpha-isopropylmalate/homocitrate synthase, conserved site
    1. PS00815 (AIPM_HOMOC...)
    2. PS00816 (AIPM_HOMOC...)
Unintegrated signatures no IPR
Unintegrated signatures
  1. SSF51569 (Aldolase)
  2. SSF89000 (post-HMGL...)
  3. cd07942 (DRE_TIM_LeuA)

Residue annotation

  1. catalytic residues...
  2. active site cd07942
  3. metal binding site...

Protein sequence

>MCA_01672_1
MLQCPCSKYRPFPPVKLPERTWPDQVLTKPPRWLSTDLRDGNQSLPDPMSVEEKKEYFKKLVDIGFKEIEVAFPSASQID
FDFTRHAVETAPEDVYIQVLTPCREELIKRTVESLQGAHKAILHMYLATSELFRDVVFGMEEEDSIALAVKCTKLVRSLT
KDAPNPSIAPSGKPTEWSFEFSPETFSDTDLGFAVRICEAVKAAWGPSEENPIIFNLPATVEMSTPNVFADQVEYFSTCI
SEREKVCISLHPHNDRGCAVAAAELAQMGGGQRVEGCLFGNGERTGNVDIVTLALNLYTQGVHPNLDFSDMMSIIDVVER
CNKIPVAPRAPYGGSLVVCAFSGSHQDAIKKGFARRQAQTGGDPKAENETPWKIPYLPLDPKDIGRTYEAVIRVNSQSGK
GGAAWIILRNLELDLPRGLQVAFSKVVQDAAEVKGRELKSNELVDLFEKQYFIEESQEGHIYALDTEEDFNIVSTGKDKR
QFQGSVVVGGVKQKVVGNGNGPISSFTDAINSIIPFGPLEVQNYHEHSIGAGTNTKAATYIEMSIANPKNKAHKIHQWGV
GIHEDVSQASIQAILSILNSIVRDYPEFTPATITNGNGKANGH

GO term prediction

Biological Process

GO:0009098 leucine biosynthetic process
GO:0019752 carboxylic acid metabolic process

Molecular Function

GO:0003824 catalytic activity
GO:0003852 2-isopropylmalate synthase activity
GO:0046912 transferase activity, transferring acyl groups, acyl groups converted into alkyl on transfer

Cellular Component

None predicted.