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Protein

MCA_01339_1

Length

448 amino acids

Gene name: TUB4

Description: Tubulin gamma chain

Browser: contigA:4189729-4191143-

RNA-seq: read pairs 723, FPKM 19.9, percentile rank 41.3% (100% = highest expression)

Protein function

Annotation:		TUB4	Tubulin gamma chain
KEGG:	K10389	TUBG	tubulin gamma
EGGNOG:	0PFDU	TUB4	Tubulin is the major constituent of microtubules. Gamma tubulin is found at microtubule organizing centers (MTOC) such as the spindle poles or the centrosome (By similarity)
SGD closest match:	S000001857	TUB2	Tubulin beta chain
CGD closest match:	CAL0000176403	TUB2	Tubulin beta chain

Protein alignments

	%id	Aln length	E-value
MIA_00456_1	80.18%	454	0.0	MIA_00456_1
A0A0J9X9R4_GEOCN	80.22%	445	0.0	Tubulin gamma chain OS=Geotrichum candidum GN=BN980_GECA06s01748g PE=3 SV=1
A0A060T8B6_BLAAD	75.33%	454	0.0	Tubulin gamma chain OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C28072g PE=3 SV=1
UniRef50_Q9Y882	67.04%	443	0.0	Tubulin gamma chain n=15 Tax=Eukaryota TaxID=2759 RepID=TBG_SCHJP
Q6CFF9_YARLI	66.08%	451	0.0	Tubulin gamma chain OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_B07381g PE=3 SV=1
A0A1E4TLU8_9ASCO	62.25%	453	0.0	Tubulin gamma chain OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_1330 PE=3 SV=1
A0A167FNJ2_9ASCO	78.51%	363	0.0	Tubulin gamma chain OS=Sugiyamaella lignohabitans GN=TUB4 PE=3 SV=1
A0A1E3PLW6_9ASCO	61.65%	412	1e-165	Tubulin gamma chain OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_45893 PE=3 SV=1
A0A1D8PC97_CANAL	36.43%	442	6e-96	Tubulin beta chain OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=TUB2 PE=3 SV=1
TBB_YEAST	36.65%	442	7e-95	Tubulin beta chain OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=TUB2 PE=1 SV=2

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0796

Protein family membership

Tubulin (IPR000217)
- Gamma tubulin (IPR002454)

Domains and repeats

Domain
1 - 271
Tubulin/FtsZ, GTPase domain (IPR003008)
Domain
249 - 443
Tubulin/FtsZ, C-terminal (IPR008280)
Domain
251 - 397
Tubulin/FtsZ, 2-layer sandwich domain (IPR018316)

1 50 100 150 200 250 300 350 400 448

Detailed signature matches

IPR000217

Tubulin

PR01161 (TUBULIN)

384 - 412

PRINTS
PR01161 (TUBULIN)

54 - 73

PRINTS
PR01161 (TUBULIN)

110 - 134

PRINTS
PR01161 (TUBULIN)

136 - 154

PRINTS
PR01161 (TUBULIN)

195 - 215

PRINTS
PR01161 (TUBULIN)

181 - 194

PRINTS
PR01161 (TUBULIN)

98 - 109

PRINTS
PR01161 (TUBULIN)

11 - 31

PRINTS
PR01161 (TUBULIN)

155 - 176

PRINTS
PR01161 (TUBULIN)

IPR002454

Gamma tubulin

cd02188 (gamma_tubulin)

3 - 439

CDD
cd02188 (gamma_tubulin)
PR01164 (GAMMATUBULIN)

33 - 53

PRINTS
PR01164 (GAMMATUBULIN)

424 - 447

PRINTS
PR01164 (GAMMATUBULIN)

341 - 364

PRINTS
PR01164 (GAMMATUBULIN)

195 - 208

PRINTS
PR01164 (GAMMATUBULIN)

118 - 133

PRINTS
PR01164 (GAMMATUBULIN)

290 - 305

PRINTS
PR01164 (GAMMATUBULIN)

253 - 264

PRINTS
PR01164 (GAMMATUBULIN)

IPR003008

Tubulin/FtsZ, GTPase domain

PF00091 (Tubulin)

4 - 216

Pfam
PF00091 (Tubulin)
SM00864 (Tubulin_4)

50 - 249

SMART
SM00864 (Tubulin_4)
G3DSA:3.40.50...

1 - 271

GENE3D
G3DSA:3.40.50.1440 (G3DSA:3.40.50.1440)
SSF52490 (Tubulin n...)

2 - 248

SUPERFAMILY
SSF52490 (Tubulin nucleotide-binding domain-like)

IPR008280

Tubulin/FtsZ, C-terminal

SSF55307 (Tubulin C...)

249 - 443

SUPERFAMILY
SSF55307 (Tubulin C-terminal domain-like)

IPR018316

Tubulin/FtsZ, 2-layer sandwich domain

PF03953 (Tubulin_C)

268 - 395

Pfam
PF03953 (Tubulin_C)
G3DSA:3.30.13...

272 - 386

GENE3D
G3DSA:3.30.1330.20 (G3DSA:3.30.1330.20)
SM00865 (Tubulin_C_4)

251 - 397

SMART
SM00865 (Tubulin_C_4)

IPR017975

Tubulin, conserved site

PS00227 (TUBULIN)

144 - 150

PROSITE patterns
PS00227 (TUBULIN)

no IPR

Unintegrated signatures

G3DSA:1.10.28...

387 - 445

GENE3D
G3DSA:1.10.287.600 (G3DSA:1.10.287.600)

Residue annotation

oligomer interface...

3R

oligomer interface
3R, 49R, 135E, 254D, 256A, 260A, 263I, 264P, 266H, 267S, 332S, 333R, 335I, 336H, 339L, 340L, 343R, 355P, 356A, 359Q, 360I, 434H, 437N, 438S
CDD
cd02188 (gamma_tubulin)

49R

oligomer interface
3R, 49R, 135E, 254D, 256A, 260A, 263I, 264P, 266H, 267S, 332S, 333R, 335I, 336H, 339L, 340L, 343R, 355P, 356A, 359Q, 360I, 434H, 437N, 438S
CDD
cd02188 (gamma_tubulin)

135E

oligomer interface
3R, 49R, 135E, 254D, 256A, 260A, 263I, 264P, 266H, 267S, 332S, 333R, 335I, 336H, 339L, 340L, 343R, 355P, 356A, 359Q, 360I, 434H, 437N, 438S
CDD
cd02188 (gamma_tubulin)

254D

oligomer interface
3R, 49R, 135E, 254D, 256A, 260A, 263I, 264P, 266H, 267S, 332S, 333R, 335I, 336H, 339L, 340L, 343R, 355P, 356A, 359Q, 360I, 434H, 437N, 438S
CDD
cd02188 (gamma_tubulin)

256A

oligomer interface
3R, 49R, 135E, 254D, 256A, 260A, 263I, 264P, 266H, 267S, 332S, 333R, 335I, 336H, 339L, 340L, 343R, 355P, 356A, 359Q, 360I, 434H, 437N, 438S
CDD
cd02188 (gamma_tubulin)

260A

oligomer interface
3R, 49R, 135E, 254D, 256A, 260A, 263I, 264P, 266H, 267S, 332S, 333R, 335I, 336H, 339L, 340L, 343R, 355P, 356A, 359Q, 360I, 434H, 437N, 438S
CDD
cd02188 (gamma_tubulin)

263I

oligomer interface
3R, 49R, 135E, 254D, 256A, 260A, 263I, 264P, 266H, 267S, 332S, 333R, 335I, 336H, 339L, 340L, 343R, 355P, 356A, 359Q, 360I, 434H, 437N, 438S
CDD
cd02188 (gamma_tubulin)

264P

oligomer interface
3R, 49R, 135E, 254D, 256A, 260A, 263I, 264P, 266H, 267S, 332S, 333R, 335I, 336H, 339L, 340L, 343R, 355P, 356A, 359Q, 360I, 434H, 437N, 438S
CDD
cd02188 (gamma_tubulin)

266H

oligomer interface
3R, 49R, 135E, 254D, 256A, 260A, 263I, 264P, 266H, 267S, 332S, 333R, 335I, 336H, 339L, 340L, 343R, 355P, 356A, 359Q, 360I, 434H, 437N, 438S
CDD
cd02188 (gamma_tubulin)

267S

oligomer interface
3R, 49R, 135E, 254D, 256A, 260A, 263I, 264P, 266H, 267S, 332S, 333R, 335I, 336H, 339L, 340L, 343R, 355P, 356A, 359Q, 360I, 434H, 437N, 438S
CDD
cd02188 (gamma_tubulin)

332S

oligomer interface
3R, 49R, 135E, 254D, 256A, 260A, 263I, 264P, 266H, 267S, 332S, 333R, 335I, 336H, 339L, 340L, 343R, 355P, 356A, 359Q, 360I, 434H, 437N, 438S
CDD
cd02188 (gamma_tubulin)

333R

oligomer interface
3R, 49R, 135E, 254D, 256A, 260A, 263I, 264P, 266H, 267S, 332S, 333R, 335I, 336H, 339L, 340L, 343R, 355P, 356A, 359Q, 360I, 434H, 437N, 438S
CDD
cd02188 (gamma_tubulin)

335I

oligomer interface
3R, 49R, 135E, 254D, 256A, 260A, 263I, 264P, 266H, 267S, 332S, 333R, 335I, 336H, 339L, 340L, 343R, 355P, 356A, 359Q, 360I, 434H, 437N, 438S
CDD
cd02188 (gamma_tubulin)

336H

oligomer interface
3R, 49R, 135E, 254D, 256A, 260A, 263I, 264P, 266H, 267S, 332S, 333R, 335I, 336H, 339L, 340L, 343R, 355P, 356A, 359Q, 360I, 434H, 437N, 438S
CDD
cd02188 (gamma_tubulin)

339L

oligomer interface
3R, 49R, 135E, 254D, 256A, 260A, 263I, 264P, 266H, 267S, 332S, 333R, 335I, 336H, 339L, 340L, 343R, 355P, 356A, 359Q, 360I, 434H, 437N, 438S
CDD
cd02188 (gamma_tubulin)

340L

oligomer interface
3R, 49R, 135E, 254D, 256A, 260A, 263I, 264P, 266H, 267S, 332S, 333R, 335I, 336H, 339L, 340L, 343R, 355P, 356A, 359Q, 360I, 434H, 437N, 438S
CDD
cd02188 (gamma_tubulin)

343R

oligomer interface
3R, 49R, 135E, 254D, 256A, 260A, 263I, 264P, 266H, 267S, 332S, 333R, 335I, 336H, 339L, 340L, 343R, 355P, 356A, 359Q, 360I, 434H, 437N, 438S
CDD
cd02188 (gamma_tubulin)

355P

oligomer interface
3R, 49R, 135E, 254D, 256A, 260A, 263I, 264P, 266H, 267S, 332S, 333R, 335I, 336H, 339L, 340L, 343R, 355P, 356A, 359Q, 360I, 434H, 437N, 438S
CDD
cd02188 (gamma_tubulin)

356A

oligomer interface
3R, 49R, 135E, 254D, 256A, 260A, 263I, 264P, 266H, 267S, 332S, 333R, 335I, 336H, 339L, 340L, 343R, 355P, 356A, 359Q, 360I, 434H, 437N, 438S
CDD
cd02188 (gamma_tubulin)

359Q

oligomer interface
3R, 49R, 135E, 254D, 256A, 260A, 263I, 264P, 266H, 267S, 332S, 333R, 335I, 336H, 339L, 340L, 343R, 355P, 356A, 359Q, 360I, 434H, 437N, 438S
CDD
cd02188 (gamma_tubulin)

360I

oligomer interface
3R, 49R, 135E, 254D, 256A, 260A, 263I, 264P, 266H, 267S, 332S, 333R, 335I, 336H, 339L, 340L, 343R, 355P, 356A, 359Q, 360I, 434H, 437N, 438S
CDD
cd02188 (gamma_tubulin)

434H

oligomer interface
3R, 49R, 135E, 254D, 256A, 260A, 263I, 264P, 266H, 267S, 332S, 333R, 335I, 336H, 339L, 340L, 343R, 355P, 356A, 359Q, 360I, 434H, 437N, 438S
CDD
cd02188 (gamma_tubulin)

437N

oligomer interface
3R, 49R, 135E, 254D, 256A, 260A, 263I, 264P, 266H, 267S, 332S, 333R, 335I, 336H, 339L, 340L, 343R, 355P, 356A, 359Q, 360I, 434H, 437N, 438S
CDD
cd02188 (gamma_tubulin)

438S

oligomer interface
3R, 49R, 135E, 254D, 256A, 260A, 263I, 264P, 266H, 267S, 332S, 333R, 335I, 336H, 339L, 340L, 343R, 355P, 356A, 359Q, 360I, 434H, 437N, 438S
CDD
cd02188 (gamma_tubulin)
nucleotide binding...

11G

nucleotide binding site
11G, 12Q, 13C, 16Q, 104N, 142S, 144A, 145G, 146G, 147T, 148G, 173V, 175P, 209N, 227L, 230T, 231N
CDD
cd02188 (gamma_tubulin)

12Q

nucleotide binding site
11G, 12Q, 13C, 16Q, 104N, 142S, 144A, 145G, 146G, 147T, 148G, 173V, 175P, 209N, 227L, 230T, 231N
CDD
cd02188 (gamma_tubulin)

13C

nucleotide binding site
11G, 12Q, 13C, 16Q, 104N, 142S, 144A, 145G, 146G, 147T, 148G, 173V, 175P, 209N, 227L, 230T, 231N
CDD
cd02188 (gamma_tubulin)

16Q

nucleotide binding site
11G, 12Q, 13C, 16Q, 104N, 142S, 144A, 145G, 146G, 147T, 148G, 173V, 175P, 209N, 227L, 230T, 231N
CDD
cd02188 (gamma_tubulin)

104N

nucleotide binding site
11G, 12Q, 13C, 16Q, 104N, 142S, 144A, 145G, 146G, 147T, 148G, 173V, 175P, 209N, 227L, 230T, 231N
CDD
cd02188 (gamma_tubulin)

142S

nucleotide binding site
11G, 12Q, 13C, 16Q, 104N, 142S, 144A, 145G, 146G, 147T, 148G, 173V, 175P, 209N, 227L, 230T, 231N
CDD
cd02188 (gamma_tubulin)

144A

nucleotide binding site
11G, 12Q, 13C, 16Q, 104N, 142S, 144A, 145G, 146G, 147T, 148G, 173V, 175P, 209N, 227L, 230T, 231N
CDD
cd02188 (gamma_tubulin)

145G

nucleotide binding site
11G, 12Q, 13C, 16Q, 104N, 142S, 144A, 145G, 146G, 147T, 148G, 173V, 175P, 209N, 227L, 230T, 231N
CDD
cd02188 (gamma_tubulin)

146G

nucleotide binding site
11G, 12Q, 13C, 16Q, 104N, 142S, 144A, 145G, 146G, 147T, 148G, 173V, 175P, 209N, 227L, 230T, 231N
CDD
cd02188 (gamma_tubulin)

147T

nucleotide binding site
11G, 12Q, 13C, 16Q, 104N, 142S, 144A, 145G, 146G, 147T, 148G, 173V, 175P, 209N, 227L, 230T, 231N
CDD
cd02188 (gamma_tubulin)

148G

nucleotide binding site
11G, 12Q, 13C, 16Q, 104N, 142S, 144A, 145G, 146G, 147T, 148G, 173V, 175P, 209N, 227L, 230T, 231N
CDD
cd02188 (gamma_tubulin)

173V

nucleotide binding site
11G, 12Q, 13C, 16Q, 104N, 142S, 144A, 145G, 146G, 147T, 148G, 173V, 175P, 209N, 227L, 230T, 231N
CDD
cd02188 (gamma_tubulin)

175P

nucleotide binding site
11G, 12Q, 13C, 16Q, 104N, 142S, 144A, 145G, 146G, 147T, 148G, 173V, 175P, 209N, 227L, 230T, 231N
CDD
cd02188 (gamma_tubulin)

209N

nucleotide binding site
11G, 12Q, 13C, 16Q, 104N, 142S, 144A, 145G, 146G, 147T, 148G, 173V, 175P, 209N, 227L, 230T, 231N
CDD
cd02188 (gamma_tubulin)

227L

nucleotide binding site
11G, 12Q, 13C, 16Q, 104N, 142S, 144A, 145G, 146G, 147T, 148G, 173V, 175P, 209N, 227L, 230T, 231N
CDD
cd02188 (gamma_tubulin)

230T

nucleotide binding site
11G, 12Q, 13C, 16Q, 104N, 142S, 144A, 145G, 146G, 147T, 148G, 173V, 175P, 209N, 227L, 230T, 231N
CDD
cd02188 (gamma_tubulin)

231N

nucleotide binding site
11G, 12Q, 13C, 16Q, 104N, 142S, 144A, 145G, 146G, 147T, 148G, 173V, 175P, 209N, 227L, 230T, 231N
CDD
cd02188 (gamma_tubulin)

Protein sequence

>MCA_01339_1
MPRELITIQTGQCGNQIGSQFWQQLCLEHGISPDGTLQTSDNSSTLRDRKDVFFYQSDDTRYIPRSILVDLEPRVVNSIM
TGSHSKMFNPDNVYISPDGGGAGNIWAAGYHAGEGISDILMDMLDREADGSDSLEGFMLLHSIAGGTGSGLGSFLLEHIN
DRFPKKLIQTYSVFPDSHQISDVVVQPYNSILTLKRLALYADSVIVLDNAALTRIAADTLKVQSPTLSQTNQLVSTVMSA
STSTLRYPGYLHNDLASIMASLIPLHSCHFLMTAYTPFSNDTVDQAKLVRKTTILDVMSRLLQPKTRMISLNPSKTGRYL
SILNIIQGEVDSREIHKSLLRIRERNLVSFIDSVPASIQISLSKRSPYLKSGAASRISGLMLANHTSMASLFQRTLDQFE
RLLKRQAFLEQYKKQPMFENGLEEFEDAREHVKHLINSYKQLEYQHTE

GO term prediction

Biological Process

GO:0007017 microtubule-based process
GO:0007020 microtubule nucleation
GO:0031122 cytoplasmic microtubule organization

Molecular Function

GO:0003924 GTPase activity
GO:0005525 GTP binding

Cellular Component

GO:0000930 gamma-tubulin complex
GO:0005874 microtubule