MCA_01279_1

Protein

Length

978 amino acids

RNA-seq: read pairs 714, FPKM 9.0, percentile rank 24.3% (100% = highest expression)

Protein function

KEGG:	K10807	RRM1	ribonucleoside-diphosphate reductase subunit M1 [EC:1.17.4.1]
EGGNOG:	0PGH5	FG05174.1	Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides (By similarity)
SGD closest match:	S000000872	RNR1	Ribonucleoside-diphosphate reductase large chain 1
CGD closest match:	CAL0000199428	RNR1	Ribonucleoside-diphosphate reductase

Protein alignments

	%id	Aln length
MIA_03526_1	73.09%	758	MIA_03526_1
UniRef50_A0A1B7SMA0	69.00%	758	Ribonucleoside-diphosphate reductase n=61 Tax=cellular organisms TaxID=131567 RepID=A0A1B7SMA0_9ASCO
A0A0J9XFZ3_GEOCN	70.47%	745	Ribonucleoside-diphosphate reductase OS=Geotrichum candidum GN=BN980_GECA14s01319g PE=3 SV=1
Q5A0N3_CANAL	67.94%	758	Ribonucleoside-diphosphate reductase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=RNR1 PE=3 SV=1
A0A1E4TDZ6_9ASCO	68.21%	758	Ribonucleoside-diphosphate reductase OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_26775 PE=3 SV=1
A0A1E3PIT4_9ASCO	67.28%	758	Ribonucleoside-diphosphate reductase OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_51452 PE=3 SV=1
A0A060T162_BLAAD	67.15%	758	Ribonucleoside-diphosphate reductase OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C21780g PE=3 SV=1
Q6BZQ3_YARLI	66.75%	758	Ribonucleoside-diphosphate reductase OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_F31735g PE=3 SV=1
RIR1_YEAST	64.87%	760	Ribonucleoside-diphosphate reductase large chain 1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=RNR1 PE=1 SV=2
A0A167FQX0_9ASCO	65.90%	692	Ribonucleoside-diphosphate reductase OS=Sugiyamaella lignohabitans GN=RNR1 PE=3 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0965

Protein family membership

None predicted.

Domains and repeats

Domain
1 - 92
ATP-cone domain (IPR005144)
145 - 744
Ribonucleotide reductase large subunit, C-terminal (IPR000788)
Ribonucleotide reductase, class I , alpha subunit (IPR013346)
Domain
6 - 214
Ribonucleotide reductase R1 subunit, N-terminal (IPR008926)
Domain
141 - 212
Ribonucleotide reductase large subunit, N-terminal (IPR013509)

1 100 200 300 400 500 600 700 800 900 978

Detailed signature matches

IPR005144

ATP-cone domain
1. PF03477 (ATP-cone)
  
  1 - 89
  
  Pfam
  PF03477 (ATP-cone)
2. PS51161 (ATP_CONE)
  
  1 - 92
  
  PROSITE profiles
  PS51161 (ATP_CONE)
IPR008926

Ribonucleotide reductase R1 subunit, N-terminal
1. SSF48168 (R1 subuni...)
  
  6 - 214
  
  SUPERFAMILY
  SSF48168 (R1 subunit of ribonucleotide reductase, N-terminal domain)
IPR013509

Ribonucleotide reductase large subunit, N-terminal
1. PF00317 (Ribonuc_re...)
  
  141 - 212
  
  Pfam
  PF00317 (Ribonuc_red_lgN)
IPR000788

Ribonucleotide reductase large subunit, C-terminal
1. PF02867 (Ribonuc_re...)
  
  216 - 741
  
  Pfam
  PF02867 (Ribonuc_red_lgC)
2. PR01183 (RIBORDTASEM1)
  
  502 - 524
  
  PRINTS
  PR01183 (RIBORDTASEM1)
  
  530 - 553
  
  PRINTS
  PR01183 (RIBORDTASEM1)
  
  595 - 622
  
  PRINTS
  PR01183 (RIBORDTASEM1)
  
  423 - 434
  
  PRINTS
  PR01183 (RIBORDTASEM1)
  
  464 - 487
  
  PRINTS
  PR01183 (RIBORDTASEM1)
  
  293 - 312
  
  PRINTS
  PR01183 (RIBORDTASEM1)
IPR013346

Ribonucleotide reductase, class I , alpha subunit
1. TIGR02506
  
  145 - 744
  
  TIGRFAMs
  TIGR02506 (TIGR02506)

no IPR

Unintegrated signatures

G3DSA:3.20.70.20

121 - 748

GENE3D
G3DSA:3.20.70.20 (G3DSA:3.20.70.20)
SSF51998 (PFL-like ...)

678 - 754

SUPERFAMILY
SSF51998 (PFL-like glycyl radical enzymes)

215 - 645

SUPERFAMILY
SSF51998 (PFL-like glycyl radical enzymes)
cd01679 (RNR_I)

167 - 744

CDD
cd01679 (RNR_I)
mobidb-lite (disord...)

833 - 978

MobiDB Lite
mobidb-lite (disorder_prediction)

Residue annotation

active site cd01679

202S

active site
202S, 203P, 217S, 218C, 247G, 427N, 429C, 431E, 446L, 605M, 606P, 607T, 608A, 609S, 610T
CDD
cd01679 (RNR_I)

203P

active site
202S, 203P, 217S, 218C, 247G, 427N, 429C, 431E, 446L, 605M, 606P, 607T, 608A, 609S, 610T
CDD
cd01679 (RNR_I)

217S

active site
202S, 203P, 217S, 218C, 247G, 427N, 429C, 431E, 446L, 605M, 606P, 607T, 608A, 609S, 610T
CDD
cd01679 (RNR_I)

218C

active site
202S, 203P, 217S, 218C, 247G, 427N, 429C, 431E, 446L, 605M, 606P, 607T, 608A, 609S, 610T
CDD
cd01679 (RNR_I)

247G

active site
202S, 203P, 217S, 218C, 247G, 427N, 429C, 431E, 446L, 605M, 606P, 607T, 608A, 609S, 610T
CDD
cd01679 (RNR_I)

427N

active site
202S, 203P, 217S, 218C, 247G, 427N, 429C, 431E, 446L, 605M, 606P, 607T, 608A, 609S, 610T
CDD
cd01679 (RNR_I)

429C

active site
202S, 203P, 217S, 218C, 247G, 427N, 429C, 431E, 446L, 605M, 606P, 607T, 608A, 609S, 610T
CDD
cd01679 (RNR_I)

431E

active site
202S, 203P, 217S, 218C, 247G, 427N, 429C, 431E, 446L, 605M, 606P, 607T, 608A, 609S, 610T
CDD
cd01679 (RNR_I)

446L

active site
202S, 203P, 217S, 218C, 247G, 427N, 429C, 431E, 446L, 605M, 606P, 607T, 608A, 609S, 610T
CDD
cd01679 (RNR_I)

605M

active site
202S, 203P, 217S, 218C, 247G, 427N, 429C, 431E, 446L, 605M, 606P, 607T, 608A, 609S, 610T
CDD
cd01679 (RNR_I)

606P

active site
202S, 203P, 217S, 218C, 247G, 427N, 429C, 431E, 446L, 605M, 606P, 607T, 608A, 609S, 610T
CDD
cd01679 (RNR_I)

607T

active site
202S, 203P, 217S, 218C, 247G, 427N, 429C, 431E, 446L, 605M, 606P, 607T, 608A, 609S, 610T
CDD
cd01679 (RNR_I)

608A

active site
202S, 203P, 217S, 218C, 247G, 427N, 429C, 431E, 446L, 605M, 606P, 607T, 608A, 609S, 610T
CDD
cd01679 (RNR_I)

609S

active site
202S, 203P, 217S, 218C, 247G, 427N, 429C, 431E, 446L, 605M, 606P, 607T, 608A, 609S, 610T
CDD
cd01679 (RNR_I)

610T

active site
202S, 203P, 217S, 218C, 247G, 427N, 429C, 431E, 446L, 605M, 606P, 607T, 608A, 609S, 610T
CDD
cd01679 (RNR_I)
dimer interface cd...

214Q

dimer interface
214Q, 228M, 229S, 232Y, 235L, 236K, 239A, 240L, 243K, 258K, 270S, 274P, 275M, 277K, 278V, 281S, 282T, 285Y, 286A, 287D, 288Q, 289G
CDD
cd01679 (RNR_I)

228M

dimer interface
214Q, 228M, 229S, 232Y, 235L, 236K, 239A, 240L, 243K, 258K, 270S, 274P, 275M, 277K, 278V, 281S, 282T, 285Y, 286A, 287D, 288Q, 289G
CDD
cd01679 (RNR_I)

229S

dimer interface
214Q, 228M, 229S, 232Y, 235L, 236K, 239A, 240L, 243K, 258K, 270S, 274P, 275M, 277K, 278V, 281S, 282T, 285Y, 286A, 287D, 288Q, 289G
CDD
cd01679 (RNR_I)

232Y

dimer interface
214Q, 228M, 229S, 232Y, 235L, 236K, 239A, 240L, 243K, 258K, 270S, 274P, 275M, 277K, 278V, 281S, 282T, 285Y, 286A, 287D, 288Q, 289G
CDD
cd01679 (RNR_I)

235L

dimer interface
214Q, 228M, 229S, 232Y, 235L, 236K, 239A, 240L, 243K, 258K, 270S, 274P, 275M, 277K, 278V, 281S, 282T, 285Y, 286A, 287D, 288Q, 289G
CDD
cd01679 (RNR_I)

236K

dimer interface
214Q, 228M, 229S, 232Y, 235L, 236K, 239A, 240L, 243K, 258K, 270S, 274P, 275M, 277K, 278V, 281S, 282T, 285Y, 286A, 287D, 288Q, 289G
CDD
cd01679 (RNR_I)

239A

dimer interface
214Q, 228M, 229S, 232Y, 235L, 236K, 239A, 240L, 243K, 258K, 270S, 274P, 275M, 277K, 278V, 281S, 282T, 285Y, 286A, 287D, 288Q, 289G
CDD
cd01679 (RNR_I)

240L

dimer interface
214Q, 228M, 229S, 232Y, 235L, 236K, 239A, 240L, 243K, 258K, 270S, 274P, 275M, 277K, 278V, 281S, 282T, 285Y, 286A, 287D, 288Q, 289G
CDD
cd01679 (RNR_I)

243K

dimer interface
214Q, 228M, 229S, 232Y, 235L, 236K, 239A, 240L, 243K, 258K, 270S, 274P, 275M, 277K, 278V, 281S, 282T, 285Y, 286A, 287D, 288Q, 289G
CDD
cd01679 (RNR_I)

258K

dimer interface
214Q, 228M, 229S, 232Y, 235L, 236K, 239A, 240L, 243K, 258K, 270S, 274P, 275M, 277K, 278V, 281S, 282T, 285Y, 286A, 287D, 288Q, 289G
CDD
cd01679 (RNR_I)

270S

dimer interface
214Q, 228M, 229S, 232Y, 235L, 236K, 239A, 240L, 243K, 258K, 270S, 274P, 275M, 277K, 278V, 281S, 282T, 285Y, 286A, 287D, 288Q, 289G
CDD
cd01679 (RNR_I)

274P

dimer interface
214Q, 228M, 229S, 232Y, 235L, 236K, 239A, 240L, 243K, 258K, 270S, 274P, 275M, 277K, 278V, 281S, 282T, 285Y, 286A, 287D, 288Q, 289G
CDD
cd01679 (RNR_I)

275M

dimer interface
214Q, 228M, 229S, 232Y, 235L, 236K, 239A, 240L, 243K, 258K, 270S, 274P, 275M, 277K, 278V, 281S, 282T, 285Y, 286A, 287D, 288Q, 289G
CDD
cd01679 (RNR_I)

277K

dimer interface
214Q, 228M, 229S, 232Y, 235L, 236K, 239A, 240L, 243K, 258K, 270S, 274P, 275M, 277K, 278V, 281S, 282T, 285Y, 286A, 287D, 288Q, 289G
CDD
cd01679 (RNR_I)

278V

dimer interface
214Q, 228M, 229S, 232Y, 235L, 236K, 239A, 240L, 243K, 258K, 270S, 274P, 275M, 277K, 278V, 281S, 282T, 285Y, 286A, 287D, 288Q, 289G
CDD
cd01679 (RNR_I)

281S

dimer interface
214Q, 228M, 229S, 232Y, 235L, 236K, 239A, 240L, 243K, 258K, 270S, 274P, 275M, 277K, 278V, 281S, 282T, 285Y, 286A, 287D, 288Q, 289G
CDD
cd01679 (RNR_I)

282T

dimer interface
214Q, 228M, 229S, 232Y, 235L, 236K, 239A, 240L, 243K, 258K, 270S, 274P, 275M, 277K, 278V, 281S, 282T, 285Y, 286A, 287D, 288Q, 289G
CDD
cd01679 (RNR_I)

285Y

dimer interface
214Q, 228M, 229S, 232Y, 235L, 236K, 239A, 240L, 243K, 258K, 270S, 274P, 275M, 277K, 278V, 281S, 282T, 285Y, 286A, 287D, 288Q, 289G
CDD
cd01679 (RNR_I)

286A

dimer interface
214Q, 228M, 229S, 232Y, 235L, 236K, 239A, 240L, 243K, 258K, 270S, 274P, 275M, 277K, 278V, 281S, 282T, 285Y, 286A, 287D, 288Q, 289G
CDD
cd01679 (RNR_I)

287D

dimer interface
214Q, 228M, 229S, 232Y, 235L, 236K, 239A, 240L, 243K, 258K, 270S, 274P, 275M, 277K, 278V, 281S, 282T, 285Y, 286A, 287D, 288Q, 289G
CDD
cd01679 (RNR_I)

288Q

dimer interface
214Q, 228M, 229S, 232Y, 235L, 236K, 239A, 240L, 243K, 258K, 270S, 274P, 275M, 277K, 278V, 281S, 282T, 285Y, 286A, 287D, 288Q, 289G
CDD
cd01679 (RNR_I)

289G

dimer interface
214Q, 228M, 229S, 232Y, 235L, 236K, 239A, 240L, 243K, 258K, 270S, 274P, 275M, 277K, 278V, 281S, 282T, 285Y, 286A, 287D, 288Q, 289G
CDD
cd01679 (RNR_I)
catalytic residues...

218C

catalytic residues
218C, 427N, 429C, 431E, 444C, 740Y, 741Y
CDD
cd01679 (RNR_I)

427N

catalytic residues
218C, 427N, 429C, 431E, 444C, 740Y, 741Y
CDD
cd01679 (RNR_I)

429C

catalytic residues
218C, 427N, 429C, 431E, 444C, 740Y, 741Y
CDD
cd01679 (RNR_I)

431E

catalytic residues
218C, 427N, 429C, 431E, 444C, 740Y, 741Y
CDD
cd01679 (RNR_I)

444C

catalytic residues
218C, 427N, 429C, 431E, 444C, 740Y, 741Y
CDD
cd01679 (RNR_I)

740Y

catalytic residues
218C, 427N, 429C, 431E, 444C, 740Y, 741Y
CDD
cd01679 (RNR_I)

741Y

catalytic residues
218C, 427N, 429C, 431E, 444C, 740Y, 741Y
CDD
cd01679 (RNR_I)
effector binding s...

226D

effector binding site
226D, 227S, 228M, 231I, 243K, 256R, 270S, 275M
CDD
cd01679 (RNR_I)

227S

effector binding site
226D, 227S, 228M, 231I, 243K, 256R, 270S, 275M
CDD
cd01679 (RNR_I)

228M

effector binding site
226D, 227S, 228M, 231I, 243K, 256R, 270S, 275M
CDD
cd01679 (RNR_I)

231I

effector binding site
226D, 227S, 228M, 231I, 243K, 256R, 270S, 275M
CDD
cd01679 (RNR_I)

243K

effector binding site
226D, 227S, 228M, 231I, 243K, 256R, 270S, 275M
CDD
cd01679 (RNR_I)

256R

effector binding site
226D, 227S, 228M, 231I, 243K, 256R, 270S, 275M
CDD
cd01679 (RNR_I)

270S

effector binding site
226D, 227S, 228M, 231I, 243K, 256R, 270S, 275M
CDD
cd01679 (RNR_I)

275M

effector binding site
226D, 227S, 228M, 231I, 243K, 256R, 270S, 275M
CDD
cd01679 (RNR_I)
R2 peptide binding...

336D

R2 peptide binding site
336D, 339M, 340E, 342V, 378E, 379R, 391Y, 398I, 719T, 720L, 721S, 723L, 724T, 728F, 729Y, 731W, 732K
CDD
cd01679 (RNR_I)

339M

R2 peptide binding site
336D, 339M, 340E, 342V, 378E, 379R, 391Y, 398I, 719T, 720L, 721S, 723L, 724T, 728F, 729Y, 731W, 732K
CDD
cd01679 (RNR_I)

340E

R2 peptide binding site
336D, 339M, 340E, 342V, 378E, 379R, 391Y, 398I, 719T, 720L, 721S, 723L, 724T, 728F, 729Y, 731W, 732K
CDD
cd01679 (RNR_I)

342V

R2 peptide binding site
336D, 339M, 340E, 342V, 378E, 379R, 391Y, 398I, 719T, 720L, 721S, 723L, 724T, 728F, 729Y, 731W, 732K
CDD
cd01679 (RNR_I)

378E

R2 peptide binding site
336D, 339M, 340E, 342V, 378E, 379R, 391Y, 398I, 719T, 720L, 721S, 723L, 724T, 728F, 729Y, 731W, 732K
CDD
cd01679 (RNR_I)

379R

R2 peptide binding site
336D, 339M, 340E, 342V, 378E, 379R, 391Y, 398I, 719T, 720L, 721S, 723L, 724T, 728F, 729Y, 731W, 732K
CDD
cd01679 (RNR_I)

391Y

R2 peptide binding site
336D, 339M, 340E, 342V, 378E, 379R, 391Y, 398I, 719T, 720L, 721S, 723L, 724T, 728F, 729Y, 731W, 732K
CDD
cd01679 (RNR_I)

398I

R2 peptide binding site
336D, 339M, 340E, 342V, 378E, 379R, 391Y, 398I, 719T, 720L, 721S, 723L, 724T, 728F, 729Y, 731W, 732K
CDD
cd01679 (RNR_I)

719T

R2 peptide binding site
336D, 339M, 340E, 342V, 378E, 379R, 391Y, 398I, 719T, 720L, 721S, 723L, 724T, 728F, 729Y, 731W, 732K
CDD
cd01679 (RNR_I)

720L

R2 peptide binding site
336D, 339M, 340E, 342V, 378E, 379R, 391Y, 398I, 719T, 720L, 721S, 723L, 724T, 728F, 729Y, 731W, 732K
CDD
cd01679 (RNR_I)

721S

R2 peptide binding site
336D, 339M, 340E, 342V, 378E, 379R, 391Y, 398I, 719T, 720L, 721S, 723L, 724T, 728F, 729Y, 731W, 732K
CDD
cd01679 (RNR_I)

723L

R2 peptide binding site
336D, 339M, 340E, 342V, 378E, 379R, 391Y, 398I, 719T, 720L, 721S, 723L, 724T, 728F, 729Y, 731W, 732K
CDD
cd01679 (RNR_I)

724T

R2 peptide binding site
336D, 339M, 340E, 342V, 378E, 379R, 391Y, 398I, 719T, 720L, 721S, 723L, 724T, 728F, 729Y, 731W, 732K
CDD
cd01679 (RNR_I)

728F

R2 peptide binding site
336D, 339M, 340E, 342V, 378E, 379R, 391Y, 398I, 719T, 720L, 721S, 723L, 724T, 728F, 729Y, 731W, 732K
CDD
cd01679 (RNR_I)

729Y

R2 peptide binding site
336D, 339M, 340E, 342V, 378E, 379R, 391Y, 398I, 719T, 720L, 721S, 723L, 724T, 728F, 729Y, 731W, 732K
CDD
cd01679 (RNR_I)

731W

R2 peptide binding site
336D, 339M, 340E, 342V, 378E, 379R, 391Y, 398I, 719T, 720L, 721S, 723L, 724T, 728F, 729Y, 731W, 732K
CDD
cd01679 (RNR_I)

732K

R2 peptide binding site
336D, 339M, 340E, 342V, 378E, 379R, 391Y, 398I, 719T, 720L, 721S, 723L, 724T, 728F, 729Y, 731W, 732K
CDD
cd01679 (RNR_I)

Protein sequence

>MCA_01279_1
MFVVKRDGRQERVQFDKITARISRLSDGLDPRYVDCALITQKVISGIYPGVSTIDLDNLAAETAATMTTIHPDYETLAAR
IVVSNLHKQTKSKFSHVVEDLHSCYNPKTKTKSSLVSEELYDVVMKHKDLIDAAIQDNNDYLFTYFGIRTLEKNYLIRIG
EHIVERPQHMFMRTAIGIHGDNIPLVLETYRLMSQKFFIHASPTLFNAGTTRPQMSSCFLVAMKDDSMSGIYDTLKTCAL
ISKHAGGIGLHVHNIRSKGSHIAGTNGTSSGLIPMLKVFNSTARYADQGGSKRPGVFAIYLEPWHGDIFDFLDIRKNHGN
EEMRARDIFIALWVPDLFMEKVSNNAEWCLFSPSDAPGLSDVYGKEFEDLYNKYEKEERYQKKVNAMDLWYAILNSQIET
GSPFIVYKDACNLKSNHKNLGTIKSSNLCTEIVQYSSPEEVAVCNLASLALPMFVDKDEKSAWFDFEALHAVTKVIVRNL
DTIIDKNHYPVPEAETSNKKHRPMAIGVQGFADLLLSLRIPFESDESRKLNTQVFETIYHGAIEASIELAKKYGPYETFK
GSPASEGQLQFDLWDHTPTDLWDWDALKSDLKKYGLRNSLLTGLMPTASTSQILGFNECFEPYTYNIYSRRVLAGEFQIT
NPWLLRDLSDLGIWNSDMKMRLLKERGSVQNIDTIPDELKTLYKTVWEIPQRAVINLALDRAPFIDQSQSMNIYMKNPTL
SKLTSMHFYSWKKGLKTGIYYLRTEAASNPIQFTVDNQAATAATAATKTKSNGDLSLSPLREKPLLDKLKLKEYIPVITN
KDKRKSRLIAIDESGERIKITKKVPKFYRRLSRSHHHHSNNNNNNNNNNTDNNHHHHHQHRHHSRNSSQSEPNGTQKKKK
REISELEPDDLKDSIDCYSENNSENQKSTKRRLGTIIKTAETETSSNSGSSGGSSMLSQPVEKTSNASSPSINNVVEEDN
STNSQCSTREASCDMCSG

GO term prediction

Biological Process

GO:0006260 DNA replication
GO:0055114 oxidation-reduction process

Molecular Function

GO:0004748 ribonucleoside-diphosphate reductase activity, thioredoxin disulfide as acceptor
GO:0005524 ATP binding

Cellular Component