Protein

MCA_01263_1

Length
541 amino acids

Gene name: THI20

Description: Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Browser: contigA:3967577-3969282-

RNA-seq: read pairs 3456, FPKM 78.8, percentile rank 75.0% (100% = highest expression)

Protein function

Annotation:THI20Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase
KEGG:K00877THI20 hydroxymethylpyrimidine/phosphomethylpyrimidine kinase / thiaminase [EC:2.7.1.49 2.7.4.7 3.5.99.2]
EGGNOG:0PHBKFG04128.1thiamin biosynthesis protein
SGD closest match:S000005416THI20Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI20
CGD closest match:CAL0000198499THI20Trifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase/thiaminase

Protein alignments

%idAln lengthE-value
MIA_04905_157.30%5480.0MIA_04905_1
A0A0J9XIZ5_GEOCN56.87%5310.0Similar to Saccharomyces cerevisiae YPL258C THI21 Hydroxymethylpyrimidine phosphate kinase OS=Geotrichum candidum GN=BN980_GECA23s00494g PE=4 SV=1
A0A060T133_BLAAD53.18%5340.0ARAD1C20988p OS=Blastobotrys adeninivorans GN=GNLVRS02_ARAD1C20988g PE=4 SV=1
UniRef50_A0A060T13353.18%5349e-180ARAD1C20988p n=5 Tax=Saccharomycetales TaxID=4892 RepID=A0A060T133_BLAAD
A0A1E3PJG6_9ASCO46.50%5149e-161Uncharacterized protein OS=Nadsonia fulvescens var. elongata DSM 6958 GN=NADFUDRAFT_52032 PE=4 SV=1
A0A167DC95_9ASCO47.18%4796e-157Putative phosphomethylpyrimidine kinase OS=Sugiyamaella lignohabitans GN=THI22 PE=4 SV=1
A0A1E4TLI3_9ASCO44.75%5141e-155Uncharacterized protein OS=Tortispora caseinolytica NRRL Y-17796 GN=CANCADRAFT_1212 PE=4 SV=1
Q6C731_YARLI49.44%5328e-155YALI0E04224p OS=Yarrowia lipolytica (strain CLIB 122 / E 150) GN=YALI0_E04224g PE=4 SV=1
A0A1D8PH02_CANAL34.66%5512e-89Trifunctional hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase/thiaminase OS=Candida albicans (strain SC5314 / ATCC MYA-2876) GN=THI20 PE=4 SV=1
THI20_YEAST34.87%5652e-84Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase THI20 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) GN=THI20 PE=1 SV=1

Mitochondrial localization by mitoprotII

Probability of mitochondrial location: 0.0252

Protein family membership

Domains and repeats

  1. Domain
  2. Domain
1 50 100 150 200 250 300 350 400 450 500 541

Detailed signature matches

  1. Family IPR027574
    Thiaminase II
    1. TIGR04306
  2. Domain IPR029056
    Ribokinase-like
    1. G3DSA:3.40.11...
    2. SSF53613 (Ribokinas...)
  3. Domain IPR013749
    Pyridoxamine kinase/Phosphomethylpyrimidine kinase
    1. PF08543 (Phos_pyr_kin)
  4. Domain IPR004399
    Hydroxymethylpyrimidine kinase/phosphomethylpyrimidine kinase
    1. cd01169 (HMPP_kinase)
    2. TIGR00097
  5. Domain IPR016084
    Haem oxygenase-like, multi-helical
    1. SSF48613 (Heme oxyg...)
    2. G3DSA:1.20.91...
  6. Domain IPR004305
    Thiaminase-2/PQQC
    1. PF03070 (TENA_THI-4)

Residue annotation

  1. dimer interface cd...
  2. substrate binding ...
  3. ATP binding site c...

Protein sequence

>MCA_01263_1
MTQDLQIPKILTIAGSDSSGGAGIEADIKTFTAHNCYGMTCITGLTAQNTLGVKDVYPVKDQTFINEALEAVNSDVGIDA
IKTGMLTSKETIETVVNFIENIKNGSSYSEEKPLFLVVDPVMVSTSGHNLISSDAIDNYVNKLIPLATVFTPNIIETESV
LKQLGKSEKVKVESLEDMKNAAKTLHSALKCQNVLVKGGHLPLYKKKSSGVLELSTSPITETGNEGDRIIIDVLYDGKEV
IEFQSQYIDSKSTHGTGCTLSSAIAANLAKGKPIKKAIQDAIHYVQTAIQTSYSIGKGNGPVNHMHNIQHRPFAAGKFLD
YLLSHPKVQPHWQRYINHEFTKKVGNNTLPLESFKYFLRQDYLYLKHYARCYGLSIYKSDDMETIKNASSVIIHIAEENK
LHIDFCAKFGISVEELESGIESLTTYAYSRYLVDVGAKEDWLSLHVAMSPCLFGYGQAANILVNEENNIKDINANPYWSW
VENYTNEGYQDAIRAGRELLETNVLKHSADRIEELVDIFATTTQMECRFWDAALAYTQEEN

GO term prediction

Biological Process

GO:0006772 thiamine metabolic process
GO:0009228 thiamine biosynthetic process

Molecular Function

GO:0008972 phosphomethylpyrimidine kinase activity
GO:0050334 thiaminase activity

Cellular Component

None predicted.